303 results about "Hydroxytyrosol" patented technology

The invention relates to a yeast for producing tyrosine and hydroxytyrosine and a construction method, and belongs to the technical field of preparation of organic compounds. The construction method comprises the following steps of introducing the sequences PcAAS and ADH into the yeast BY4741, so as to obtain the recombinant yeast PcAAS-ADH for producing the tyrosine; introducing a pdc1 gene knockoff cassette and a tyrA expression cassette into the recombinant yeast PcAAS-ADH, so as to obtain the recombinant yeast PcAAS-ADH-deltapdc1-tyrA for producing the tyrosine; introducing the DNA (deoxyribonucleic acid) sequence of HpaBC into the recombinant yeast PcAAS-ADH-deltapdc1-tyrA, so as to obtain the recombinant yeast PcAAS-ADH-HpaBC-deltapdc1-tyrA for producing the hydroxytyrosine. The tyrosine or hydroxytyrosine biological synthesis route is constructed in the yeast BY4741, so that the output of the tyrosine or hydroxytyrosine is increased.

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

The invention discloses a composition with an anti-glycosylation effect and an application thereof. The anti-glycosylation composition consists of the following components in parts by mass: 0.1 to 5 parts of an osmanthus fragrans flower extract, 0.1 to 5 parts of a pomegranate extract and 0.01 to 0.1 part of a canarium album fruit extract, wherein the mass content of polyphenol in the osmanthus fragrans flower extract is greater than or equal to 10 percent, the mass content of verbascoside in the osmanthus fragrans flower extract is greater than or equal to 10 percent, the mass content of polyphenol in the pomegranate extract is greater than or equal to 30%, the mass content of punicalagin in the pomegranate extract is greater than or equal to 8%, wherein the mass content of polyphenol inthe canarium album fruit extract is greater than or equal to 10%, and the mass content of hydroxytyrosol in the canarium album fruit extract is greater than or equal to 3%. Through the synergistic effect of the osmanthus fragrans flower extract, the pomegranate extract and thecanarium album fruit extract which have a specific proportion and specific quality, anti-glycation capacity of skins is improved in three aspects that generation of a fibroblast glycosylation reaction end product CML is remarkably reduced, generation of AGEs is reduced, and SOD expression is increased.