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82 results about "Ketoacid decarboxylase" patented technology

Acetyl-coa producing enzymes in yeast

The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
Owner:DSM IP ASSETS BV

Method of generating 1,2,4-butantriol by in vitro enzyme reaction and application thereof

ActiveCN104450798AAchieve synthesisPrecise control of dosageOxidoreductasesFermentationKetoacid decarboxylase1,2,4-Butanetriol
The invention discloses a method of generating 1,2,4-butantriol by the in vitro enzyme reaction and application thereof, and belongs to the technical field of bioengineering. The method provided by the invention comprises the following steps: respectively constructing genetically-engineered bacteria of over-expressed D-xylonic acid anhydrase genes, 2-keto acid decarboxylase genes and alcohol dehydrogenase genes; after carrying out fermentation cultivation on the obtained genetically-engineered bacteria, crushing thalli by ultraviolet waves; collecting crude enzyme fluid; and after carrying out mixed adjustment on concentration of D-xylonic acid anhydrase, 2-keto acid decarboxylase and alcohol dehydrogenase, adding a reaction substrate to synthesize 1,2,4-butantriol. The method provided by the invention realizes synthesis of 1,2,4-butantriol in vitro by controlling the enzyme reaction and using D-xylonic acid as the raw material and has the characteristic of good convenience for enlarging production; and the enlarged yield can reach 5.98g / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Recombinant escherichia coli, preparation method and method for synthesizing 3,4-dihydroxybutyric acid

The invention relates to a recombinant escherichia coli, a preparation method and a method for synthesizing 3,4-dihydroxybutyric acid, and belongs to the field of biosynthesis. The recombinant escherichia coli is obtained through knocking out xylose isomerase gene xylA, 2-keto acid aldolase gene yjhH, 2-keto acid aldolase gene yagE and alcohol dehydrogenase gene in escherichia coli, overexpressing xylose dehydrogenase gene and/or 2-keto acid decarboxylase gene, and overexpressing aldehyde dehydrogenases gene. By taking xylose as a basic substrate, a compound substrate can be formed by adding glucose and/or glycerinum on the basis of the xylose, and the 3,4-dihydroxybutyric acid is biologically synthesized through the recombinant escherichia coli; by-products are extremely less, and the formation of the by-products can be interdicted through passage metabolism optimization. The recombinant escherichia coli, the preparation method and the method for synthesizing the 3,4-dihydroxybutyric acid have the advantages that the preparation method is simple, the production cycle is short, the cost is low, later continuous optimizing and transformation are realized, and the good industrial development and application prospect is realized.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Genetically engineered bacteria and application thereof in production of BT (D-1,2,4-butanetriol)

The invention discloses genetically engineered bacteria and an application thereof in production of BT (D-1,2,4-butanetriol). The genetically engineered bacteria are novel genetically engineered bacteria which are obtained as follows: a 2-keto acid decarboxylase gene MalC, an xylose dehydrogenase gene XylB, an xylonic acid dehydratase gene YjhG and an alcohol dehydrogenase gene YqhD are constructed, cloned and expressed, the genes are transferred into cells of host bacteria BL21(DE3), genetically engineered bacteria BL21-02 are obtained, and new xylonic acid dehydratase is screened on the basis of the genetically engineered bacteria; the genetically engineered bacteria are subjected to fermenting culture for production of BT. The capability of synthesizing BT from D-xylose can be improvedby screening the provided xylonic acid dehydratase gene CcXylD. The optimal xylonic acid dehydratase gene CcXylD and alpha-keto acid decarboxylase gene KdcA from lactococcus lactis are applied to theproduction process of BT, the optimal strain BL21-15 is obtained, and finally, the BT yield can reach 10.66 g / L.
Owner:NANJING UNIV OF TECH

Method for the preparation of diols

The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite. The invention also concerns a modified microorganism for the production of an aliphatic diol.
Owner:METABOLIC EXPLORER
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