129 results about "Bioproduction" patented technology

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.

The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

Disclosed is a novel net member for supporting one or more cell constructs in the culture chamber of a bioreactor, which comprises an array of impermeable pyramidal elements protruding from the face thereof, wherein each of the corners of the base of each of said impermeable pyramidal elements comprises a circular opening. This net member provides for uniform and unobstructed flow of culture medium through 3D cell constructs in culture.Also disclosed are a bioreactor and bioreactor system incorporating said net member.The bioreactor and bioreactor system may be used in the bioproduction of therapeutic proteins and stem cell expansion.

The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and / or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

The present invention discloses a kind of P450BM-3Glu435Thr variant gene capable of catalyzing indole to generate indigo blue, and the gene is obtained by means of saturated mutation and directional evolution technology mediated random mutation and screening. The P450BM-3Glu435Thr variant gene has base sequence as shown in SEQ ID No. 1 of the sequence list, and the aspartic acid codon in the 435-th site of the original P450BM-3(F87V / A74G / L188Q) gene mutated into threonine codon. The enzymic kinetic curve measurement shows that the variant enzyme the obtained variant gene expresses has catalysis efficiency obviously higher than that in available technology. The present invention provides new practical enzyme for the biological synthesis of dye indigo blue and wide application foreground for the biological production of dye indigo blue.

This invention relates to the bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by subjecting a starting material comprising glucose, L-phenylalanine, substituted L-phenylalanine, styrene or substituted styrene to a plurality of enzyme catalyzed chemical transformations in a one-pot reaction system, using recombinant microbialcells overexpressing the enzymes. To produce phenylacetaldehyde from styrene, the cells are modified to overexpress styrene monooxygenase (SMO) and styrene oxide isomerase (SOI). To produce phenylacetic acid from styrene, SMO, SOI and aldehyde dehydrogenase are overexpressed. Alternatively, to produce 2-phenylethanol, SMO, SOI and aldehyde reductase or alcohol dehydrogenase are overexpressed, while to produce phenylethylamine, SMO, SOI and transaminase are overexpressed.

The invention discloses an alpha lipoic acid biosynthesis method, an engineered strain and a preparation method thereof. The biosynthesis method is as below: cloning an Escherichia coli lipD gene, an lplA gene, a metK gene and a lipA lipoic acid synthase gene; constructing a lipoic acid structural domain protein overexpression vector; cloning lplA into the vector highly expressing structural domain protein in series way structure to ensure full caprylyl; constructing a lipA expression vector or expression vector in series connection with metK; transforming the 4 vectors into appropriate Escherichia coli strains, regulating and comparing the medium composition and culture conditions, and promoting the conversion of caprylyl structural domain protein to lipoyl. The preparation method of alpha-lipoic acid provided by the invention employs combined low value chemical raw materials and pure biological production method, and has the advantages of little toxic substances in the production process, less pollution to the environment, high safety of the synthesized lipoic acid and high yield; and the alpha-lipoic acid has high activity.

A method of biologically producing p-hydroxybenzoic acid, and a method for producing p-hydroxybenzoic acid from lignin through chemical and biological conversion.

Provided is a method for producing L-threonine using a microorganism. In this method, the threonine dehydratase (tdc) gene present in the genomic DNA of the microorganism is partially inactivated by recombinant technology. TDC gene specificity involved in one of four threonine metabolic pathways for a microorganism with enhanced activity of a threonine operon-containing enzyme and phosphoenolpyruvate carboxylase (ppc) gene The ground is inactivated, thereby significantly increasing the production of L-threonine.

Provided are novel methods for making cellulose nanocomposites, comprising biosynthesis of cellulose fibrils in situ using a growth medium comprising a polymer matrix material, under conditions suitable to provide for dispersion of the fibril throughout the growth medium as the fibrils are being formed to provide a cellulose nanocomposite material or film wherein the cellulose fibrils are highly or uniformly dispersed in the cellulose nanocomposite material, and wherein fibril structure and / or nanocomposite composition is customizable. Certain method aspects further comprise removing or separating the cellulose nanocomposite material or film from the medium, and may further comprise washing the cellulose nanocomposite material or film to remove residual medium. Particular aspects further comprise freeze-drying the cellulose nanocomposite material or film, and / or further comprise forming a molded product using the cellulose nanocomposite material or film. Compositions made by the methods are provided.

Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.