The invention relates to a method for increasing the activity of
enterococcus faecium
Glutamate Decarboxylase by using D101 macroporous adsorption resin, and belongs to the technical field of
biology. According to the method disclosed by the invention, the D101 microporous adsorption resin is used as an
enzyme activity accelerator of
enterococcus faecium GAD, and a D101 microporous adsorption resin-GAD composite catalytic
system is constructed according to a proportion that the
mass of the D101 macroporous adsorption resin to the volume of a substrate solution to the volume of an
enterococcus faecium bacterial suspension solution or a GAD free
enzyme solution is 1 to 1 to 1; when the D101 microporous adsorption resin-GAD composite catalytic
system is subjected to reaction in a water bath oscillator of which the temperature is 37 to 43 DEG C at 80r / min or stirring reaction in a stirring tank at a
low speed for 24 to 36 hours, the yield of GABA (Gamma-
Aminobutyric Acid) can be increased by 18.54 to 149.11 percent; the D101 macroporous adsorption resin is capable of remarkably increasing the activity of the GAD, an exchange adsorption function of the D101 microporous adsorption resin on the GABA is also a purifying process of the GABA, a downstream extracting and purifying technology is simplified, and the production cost is reduced; the method is simple, green and environment-friendly.