31 results about "3-Hydroxypropionate" patented technology

Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.

Organisms are provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase β-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poly (3-hydroxyalkanoate) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3-hydroxypropionate and 3-hydroxyvalerate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3 propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, acrylics, malonic acid, esters and amines.

Several novel PHA polymer compositions produced using biological systems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

The invention relates to a catalytic agent for preparing 1,3-PDO by performing hydrogenization on 3-hydracrylic acid methyl ester. The catalytic agent consists of the following components in percentage by weight: 55 to 80 wt% of CuO, 2 to 5 wt% of MnO2, 0.1 to 2 wt% of MoO3, 0.1 to 2 wt% of P2O5 and 15 to 40 wt% of SiO2. The catalytic agent prepared by the invention is used for the technology of preparing the 1,3-PDO by performing hydrogenization on the 3-hydracrylic acid methyl ester, the reaction condition is mild, the requirement on the content of organic acid and moisture in the raw material of the 3-hydracrylic acid methyl ester is low (less than 0.5 wt%), the selectivity of the 1,3-PDO is improved, the selectivity of the 1,3-PDO and ester exchange products is reduced, and meanwhile the catalytic agent is suitable for preparing the 1,3-PDO by performing hydrogenization on the 3-hydracrylic acid methyl ester in high concentration; the conversion rate of the 3-hydracrylic acid methyl ester is larger than 99%, and the yield coefficient of the 1,3-PDO is larger than 85%.

Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.

There is described a method for producing 3-hydroxypropanal, the method comprising: culturing an Acetobacter lovaniensis bacterium in a growth medium containing phosphate at a level which is more than 1 g / liter and nitrate at a level which is more than 0.1 g / liter, wherein culturing of the bacterium produces the 3-hydroxypropanal. The 3-hydroxypropanal can be separated from the growth medium or, when the microorganism has converted some or all of the 3-hydroxypropanal to 3-hydroxypropionic acid and / or a 3-hydroxypropionate ester, it may be separated as 3-hydroxypropionic acid or a 3-hydroxypropionate ester. The separated product can be converted into other chemicals such as an ester of 3-hydroxypropionic acid, 3-hydroxypropionic acid, 3 -hydroxypropionate salts (including ammonium, sodium and calcium 3-hydroxypropionate), acrylic acid, acrylates, acrylamide, acrylonitrile, acrolein and 1,3 propanediol.

The invention relates to a synthesis method of 3-hydroxy propionate. The method mainly solves the problem that the selectivity of the 3-hydroxy propionate in the prior art is low. The synthesis method of the 3-hydroxy propionate comprises the following steps: i, enabling the ligand as shown in the formula (I) to perform the complexing reaction with cobalt carbonyl in a solvent to obtain a catalyst solution, wherein the solvent is the alcohol or the solvent containing the alcohol; R1 to R3 are independently selected from one of alkyl or alkenyl, aryl or substituted aryl, and heterocyclo, and R1 to R3 independently contain 1 to 6 carbon atoms; ii, adding ethylene oxide and carbon monoxide in the catalyst solution, reacting to obtain the 3-hydroxy propionate. The technical scheme well solves the technical problem, and can be used for the industrial production of the 3-hydroxy propionate. The the formula (I) is shown in the description.

The invention relates to a method for synthesizing 3-hydracrylic acid ester. The method mainly solves the problem that in the prior art, catalysts in a homogeneous system are difficult to separate. The method for synthesizing 3-hydracrylic acid ester includes the following steps that firstly, a ligand and cobalt carbonyl are subjected to a coordination reaction in an alcohol solvent to obtain turbid liquid of a catalyst; secondly, ethylene oxide and carbon monoxide are added, and a reaction is conducted to obtain 3-hydracrylic acid ester. According to the technical scheme, the ligand is selected from 4-vinylpyddine homopolymers or copolymers containing 4-vinylpyddine monomeric units, the technical problem is well solved, and the method can be used in industrial production of 3-hydracrylic acid ester.

The invention relates to a method for preparing 3-hydroxypropionate and mainly aims to solve the problem that in the prior art, a catalyst in a homogeneous system is hard to separate. The method for preparing 3-hydroxypropionate comprises the steps that first, a ligand and cobalt carbonyl are subjected to a coordination reaction in an alcoholic solvent to obtain turbid liquid of a catalyst; second, ethylene oxide and carbon monoxide are added, and a reaction is carried out to obtain 3-hydroxypropionate. According to the technical scheme that the ligand is an acrylonitrile homopolymer or a copolymer which contains acrylonitrile monomer units, the technical problem is well solved, and the method can be applied to industrial production of 3-hydroxypropionate.

The invention relates to a process for preparing 1,3-propylene glycol (1,3-PDO) by means of hydrogenating high-concentration 3-methyl lactate (3-MHP). The process comprises the following flow: gasifying 3-MHP and H2 in a mixed manner, feeding the mixed gas into a first hydrogenation reactor, feeding a hydrogenation product into a gas-liquid separator to separate mixed gas of a heavy component I, hydrogen and a light component I; feeding the mixed gas into a first separating tank to separate H2 out for recycling, and feeding a retaining liquid phase into a first rectification tower for separating into a light component II and a heavy component II; feeding the heavy component II and the heavy component I into a second hydrogenation reactor for further hydrogenating, feeding a hydrogenation product into a second separating tank for separating H2 out for recycling, feeding remaining components into a second rectification tower into a light component III and crude 1,3-PDO, and refining the crude 1,3-PDO to obtain a 1,3-PDO product; separating the light component II from the light component III to obtain methyl alcohol and n-propyl alcohol. In the entire process, the high-concentration 3-MHP is used for hydrogenating, so that the energy consumption of solvent separation is lowered. Meanwhile, by two-segment hydrogenation is performed, the yield of the 1,3-PDO is increased.