Preparation method of long-circulation lipidosome of olive polyphenol extract rich in hydroxytyrosol and verbascoside

Abstract

The invention discloses a preparation method of long-circulation lipidosome of an olive polyphenol extract rich in hydroxytyrosol and verbascoside. Olive leaf is taken as a raw material, and is prepared into an olive polyphenol extract by extracting with 30 to 50 percent of ethanol, separating with a ceramic membrane and an ultrafiltration membrane and concentrating with a nanofiltration membrane; 90 percent of the hydroxytyrosol, 10 to 40 percent of an oleuropein extract and 90 percent of the verbascoside are compounded to prepare the olive polyphenol extract rich in the hydroxytyrosol and the verbascoside; the hydroxytyrosol and verbascoside polyphenol as model materials, lecithin, cholesterol, a Tween-80 surfactant and the like are prepared into the corresponding lipidosome. The lipidosome has the advantages that the bioavailability of the olive polyphenol extract is remarkably increased, and high encapsulation efficiency, uniform particle size distribution, good stability, slow release property, oxidation resistance and an antibacterial effect are achieved. The preparation method is simple and feasible, equipment is simple, and the application of the lipidosome in the medicine field can be widened.

Description

[0001] 1. Technical field [0002] The invention relates to the fields of biology, chemistry and medicine, in particular to a preparation method of hydroxytyrosol liposome. [0003] 2. Background technology [0004] Liposomes are mainly bilayer hollow spheres composed of lipid materials, which have both hydrophilic and hydrophobic properties, so they can encapsulate both fat-soluble substances and water-soluble substances. When the lipid material is dispersed in water, the water-soluble substances will be spontaneously distributed inside the liposome, while the fat-soluble substances will be distributed at the lipophilic end of the liposome. Liposome preparation methods mainly include: film dispersion method, ultrasonic dispersion method, freeze-thaw method, ethanol injection method, reverse phase evaporation method, etc. The evaluation indexes of its physical and chemical properties mainly include: liposome average particle size and particle size distribution, encapsulation e...

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Problems solved by technology

[0007] Literature at home and abroad have reported the extraction and separation of polyphenolic compounds such as oleuropein, hydroxytyrosol, verbascoside and tyrosol from olive leaves or olive fruits, but most of the olive polyphenols extracted from olive leaves are 10-40% Oleuropein extract with less than 10% hydroxytyrosol and less than 5% verbascoside, limiting multifunctional development of oleaginous polyphenol activity

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