The invention provides an extraction method for
plant condensed tannin high polymers, which comprises the following steps: after being freeze-dried, a
plant sample which is washed clean is ground andthen added with 70 percent of
aqueous acetone solution; after ultrasonic extraction, centrifuging and rotary
evaporation, extracted crude
tannin extract is then extracted and degreased, and after
organic solvent is removed, the remaining aqueous phase part is then freeze-dried; obtained crude
tannin extract is dissolved into water and then loaded onto a first
chromatographic column,
distilled water, 60 percent
methanol solution, 100 percent
methanol solution and 70 percent
acetone solution are sequentially adopted for washing, eluent with 70 percent
acetone is collected and loaded onto a second
chromatographic column, three types of mixtures of
acetone,
methanol and water which are composed according to different proportions are adopted for
elution for three times, the three eluents are respectively collected, and thereby the
condensed tannin high polymers with different
polymerization degree peaks are obtained. The extraction method can effectively purify high-
polymerization-degree
condensed tannin in a
plant, and provides a new approach for the field of the current research of structures and activities of macromolecular plant polyphenols.