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Method for extracting hydroxytyrosol from olive leaves

A technology of hydroxytyrosol and olive leaf, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low product purity and complicated process, achieve high product purity, reduce production cost, The effect of saving manpower and material resources

Inactive Publication Date: 2012-10-03
GUILIN SANBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method described in this invention overcomes the shortcomings of using acid solution on the column and acid solution elution, which will cause corrosion to the resin equipment, but in this method, the extraction and hydrolysis steps are carried out separately, and the pH of the extraction solution needs to be adjusted after extraction. Carry out hydrolysis again after 3, and technology is comparatively numerous and complicated; In addition, the purity of this method gained product is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Pulverize the dried olive leaves to 10 mesh, weigh 50 kg and place it in an extraction tank, add 8 times the weight of the raw material with a pH=11 aqueous sodium hydroxide solution, extract at 80°C for 8 hours, filter, and add the raw material to the filter residue. 8 times the weight of sodium hydroxide aqueous solution with pH=11, heat preservation and extraction at 80°C for 8h, filter, and combine the filtrates to obtain an extract;

[0023] 2) Adjust the pH value of the extract to 6 with hydrochloric acid, concentrate to 2 Baume, centrifuge, pass the supernatant through the HPD-100 macroporous adsorption resin column, wash the resin column with water until the effluent is clear, and then use a volume concentration of 30. The column was washed with % ethanol, followed by thin-layer chromatography for detection, and the alcohol eluent containing the target components was collected.

[0024] 3) the obtained alcohol eluent is concentrated to the extract (22 Baume),...

Embodiment 2

[0026] 1) Pulverize the dried olive leaves to 20 mesh, weigh 50 kg and place it in an extraction tank, add sodium carbonate aqueous solution with pH=12 that is 6 times the weight of the raw material, extract at 100°C for 3 hours, filter, and add the weight of the raw material to the filter residue. 6 times of sodium carbonate aqueous solution with pH=12 was incubated at 80°C for extraction for 3h, filtered, and the filtrates were combined to obtain an extract;

[0027] 2) Adjust the pH of the extract to 7 with hydrochloric acid, concentrate to 1 Baume, filter with a 100-mesh stainless steel mesh, pass the filtrate through an XDA-7 macroporous adsorption resin column, wash the resin column with water until the effluent is clear, and then use The column was washed with methanol with a volume concentration of 35%, followed by thin-layer chromatography for tracking detection, and the alcohol eluent containing the target components was collected.

[0028] 3) the obtained alcohol el...

Embodiment 3

[0030] 1) Pulverize the dried olive leaves to 18 mesh, weigh 100 kg and place it in an extraction tank, add 10 times the weight of the raw material with a pH=14 calcium hydroxide aqueous solution, heat and extract at 90°C for 8h, filter, and the filtrate is the extraction liquid;

[0031] 2) Adjust the pH value of the extract with acetic acid to 6.5, concentrate to 1 Baume, filter with a 100-mesh stainless steel mesh, pass the filtrate through LSA-21 macroporous adsorption resin column, first wash the resin column with water until the effluent is clear, and then use The column was washed with ethanol with a volume concentration of 20%, followed by thin-layer chromatography for tracking detection, and the alcohol eluent containing the target components was collected.

[0032] 3) the obtained alcohol eluent is concentrated to the extract (20 Baume), the gained extract is extracted 3 times with ethyl acetate, the consumption of ethyl acetate is 5 times of the extract weight durin...

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Abstract

The invention discloses a method for extracting hydroxytyrosol from olive leaves and belongs to the technical field of plant active ingredient extraction. The method disclosed by the invention is characterized in that the olive leaves are used as raw materials, alkali liquor with the pH value being 11 to 14 is used for carrying out heat insulation extraction under the condition of 80 to 100 DEG C, obtained extraction liquid is concentrated and passes through a macroporous adsorption resin column, methyl alcohol or ethanol is used for washing the column, the thin layer chromatography tracking detection is carried out, and alcohol washing eluent is collected; and the alcohol washing eluent is concentrated, extracting agents are used for extraction, extraction phases are collected, concentration and drying are carried out, and the hydroxytyrosol is obtained. Compared with the prior art, the method has the advantages that the olive leaves are subjected to heat insulation extraction by the alkali liquor with the pH value being 11 to 14 under the condition of 80 to 100 DEG C, the extraction and hydrolysis steps are simultaneously carried out, the extraction efficiency is high, and in addition, the hydrolysis reaction is complete; and on the other hand, the extraction and hydrolysis steps can be synchronously carried out, and the transition step is not needed between the two steps, so the work procedure is simplified, the labor and the material are saved, the production cost is reduced, and the industrial production is easily realized.

Description

technical field [0001] The invention relates to a method for extracting active components from plants, in particular to a method for extracting hydroxytyrosol from olive leaves. Background technique [0002] Hydroxytyrosol (Hydroxytyrosol) chemical name is 3,4-dihydroxyphenylethanol, is a polyphenolic substance, molecular formula C 8 H 10 O 3 , the relative molecular weight of 154. [0003] Hydroxytyrosol has safe and excellent antioxidant capacity, as well as a variety of biological and pharmacological activities such as antibacterial, anticancer, and anti-inflammatory, which can promote bone development and prevent cardiovascular and cerebrovascular diseases. In terms of anti-oxidation: it can promote metabolism, promote wound healing, eliminate free radicals in the body, restore the health of human organs, prevent brain failure, delay aging, and maintain youthful vitality; it can also protect human retinal epithelial cells, prevent and treat retinal macular disease, p...

Claims

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Application Information

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IPC IPC(8): C07C39/11C07C37/055C07C37/70
Inventor 卢照凯卢昶年钟德品谢冬养陆美珍
Owner GUILIN SANBAO PHARMA
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