The invention relates to a preparation method of a fruit concentrate Maillard reaction product, and an application of the product in tobacco perfuming. The preparation method comprises the following steps: mixing a fruit concentrate or the fruit concentrate containing reducing monosaccharide with amino acids and a reaction solvent, using an aqueous solution of sodium hydroxide to adjust the pH value of a reaction system to 6-9, heating to 80-100DEG C within 20-90min, continuously reacting for 0.5-1.5h, and cooling to obtain the corresponding reaction product. The Maillard reaction product generated after reacting the fruit concentrate with the amino acids is rich in the natural perfume of fruits, can be naturally coordinates with the original perfume of tobacco, and is safe and reliable. The above reactive perfume prepared in the invention is used to process cut tobacco under certain conditions, and is added to tobacco according to different proportions, so the smoking quality of the the prepared cut tobacco is substantially improved. The fruit concentrate Maillard reaction product can improve the fullness and the softness of the smoke of cigarette, improves the smoking smell comfortableness, and highlights the delicate perfume style of the cigarette.