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Method for extracting and purifying xanthophyl from chlorella algae powder

A technology of lutein and chlorella, applied in the biological field, can solve the problems of inability to exert medicinal value, poor water solubility of lutein, etc.

Inactive Publication Date: 2008-02-27
UNIV OF SCI & TECH BEIJING +1
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Problems solved by technology

Due to the very poor water solubility of lutein, lutein ester derivatives cannot be directly absorbed and metabolized by the human body, and cannot exert medicinal value. Under the action of enzymes in the human body, lutein esters are hydrolyzed into free lutein, and then be absorbed and participate in metabolism

Method used

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  • Method for extracting and purifying xanthophyl from chlorella algae powder
  • Method for extracting and purifying xanthophyl from chlorella algae powder

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Embodiment Construction

[0021] 1. Chlorella algae powder experimental material: the algal species used in the experiment of the present invention is a chlorella-USTB01 (Chlorella sp) that we screened ourselves and can grow heterotrophically without light, cgmcc N o 1448, date of deposit: August 25, 2005. After 2 days of heterotrophic fermentation, the cultured Chlorella was spray-dried to obtain algal powder.

[0022] 2. Extract lutein from chlorella algae powder: Add 0.2g of algae powder and 8ml of mixed extraction reagent into a centrifuge tube, and let it stand at room temperature for 1 hour.

[0023] 3. Transformation of lutein esters: add 20% sodium hydroxide to the extracted centrifuge tube, saponify at 50°C for 6 hours, and then take the upper organic phase for rotary evaporation at 43°C for 8 minutes. Use n-hexane to dissolve the lutein attached to the wall of the bottle, put it in a refrigerator at -8°C to crystallize overnight, and obtain lutein with high purity.

[0024] 4, the determina...

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Abstract

The invention discloses an extracting and purifying method of lutein from pellet algae in the biological technical domain, which comprises the following steps: extracting lutein and ester through composite agent; using 5-20% sodium hydroxide to saponify; rotating; evaporating; washing; crystallizing; obtaining high-purity lutein crystal; weighing 0. 2-2g algae powder to place in the 50-500ml centrifuge tube; adding 8. 0-80. 0ml composite liquid solvent to extract under 10-40 deg. c for 0. 5-2h; adding 10-100ml 5%-30% sodium hydroxide solution into the test tube with hybrid extractant; saponifying at 30-70 deg. c for 3-8h; centrifuging under low temperature for 5-10 min; extracting the product from organic phase. The invention ensures the optimum saponifying technique, which is extracted from pellet algae cell effectively.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for extracting and purifying lutein from chlorella algae powder, which has important application value and prospect in producing lutein, an important vital active substance, by using chlorella. Background technique [0002] Lutein belongs to an asymmetric dihydroxy carotenoid and is the main component of the macular pigment in the retina of the human eye. Current studies have found that lutein mainly has the following three aspects of medical value: 1) preventing macular degeneration in the elderly; 2) having anti-cancer effects; 3) preventing and treating cardiovascular and cerebrovascular diseases. In addition, as a high-value pure natural pigment, lutein can be widely used in industries such as food coloring, cosmetics and medicine. At present, the price of lutein is basically equal to the price of gold. It has the reputation of food gold. Its annual demand in ...

Claims

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Application Information

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IPC IPC(8): C07C403/24A61K36/05
Inventor 闫海刘硕张宾许倩倩陆钢张明忠
Owner UNIV OF SCI & TECH BEIJING
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