136 results about "Phloretin" patented technology

The invention discloses a whitening mask which is prepared by dipping a liquid prepared from the following raw materials in parts by weight: 0.1-3 parts of micromolecular hyaluronic acid, 0.1-2.5 parts of macromolecular hyaluronic acid, 0.7-30 parts of synthetic egg white powder, 1-7 parts of an aloe extract, 0.7-9 parts of propylene glycol, 5-50 parts of 1,2-butanediol, 5-30 parts of glycerinum, 40-80 parts of a ginseng extract liquid, 0.1-1 part of glabridin, 0.3-2 parts of alpha-arbutin, 0.1-1 part of phloretin, 0.1-1 part of apple stem cells, 1-5 parts of raspberry ketone glucoside, 0.1-1 part of tetrahydropiperine, 1-10 parts of vitamin c ethyl ether, 1-5 parts of tranexamic acid, 1-10 parts of hydrolyzed pearl and added to 1000 parts of water on mask paper. The whitening mask has the efficacies of promoting skin absorption of various effective components, repairing damaged cells, activating resting cells, promoting cell regeneration, moisturizing the skin, rapidly and efficiently whitening the skin and removing freckles, and is free of irritation, allergy or damage to the skin.

The present invention relates to the production of malic polyphenol as one polyphenol mixture with apple peel slag. Apple peel pomace is used as material in extracting malic polyphenol mixture through the technological processes of extraction, adsorption, separation, spray drying or decompression drying. The malic polyphenol mixture consists of catechin, epicatechin, phloretin and proanthocyanin,has several physiological activity and may be used in antioxidant, antimutagent and anomalism resisting medicine. Using apple peel pomace to replace apple in producing malic polyphenol can reduce production cost.

An anti-aging composition containing: (a) at least one first active ingredient chosen from phloretin and its derivatives; (b) at least one second active ingredient chosen from cinnamic acid, resveratrol, retinol, ascorbic acid, tocopherol, and their derivatives; and (c) at least one non-aqueous organic solvent, with the proviso that if the composition contains ascorbic acid, water is present as a co-solvent.

Based on the fact of the apple industry and the agricultural production in China, the integrated extracting and purifying method for active ingredients of apple pomace, according to the social demands and the self-research conditions, positively responses to the national policy to call for the spread of a recycling economy development model of resource-product-waste-resource for the apple residue resource current situation, constructs a chain circuit economic structure, performs integrated extraction and purification of biological wax, pectin, microcrystalline cellulose, apple polysaccharide, apple acid, apple polyphenol, phloridzin and phloretin from the apple pomace, namely the apple functional components, and overcomes the shortcoming of ''selecting the general and discarding the essence'' of the traditional extraction.

A method of inhibiting premature aging of human skin caused by exposure to infrared radiation comprising contacting the skin with a composition containing a therapeutically-effective amount of at least one protective compound chosen from baicalin, phloretin and neohesperidin dihydrochalcone, as well as combinations thereof.

The invention provides a method for preparing phloretin. The method comprises the following steps of: heating, hydrolyzing and purifying to obtain the phloretin under the action of dilute acid by using naringin dihydrochalcone. The naringin dihydrochalcone is prepared by the following method: performing catalytic hydrogenation on naringin under the medium pressure, normal temperature or heating action by using hydrogen in the presence of alkali liquor and rare metal catalyst, and collecting the naringin dihydrochalcone from the reaction system. The method is easy to operate, low in cost and high in product quality, and large-scale application of phloretin on the medicine, food and cosmetics can be well met.

This invention is to provide multiple specific inhibitors of cytochrome P450 isozyme CYP2C9. These inhibitors can be derived from any combinations with the following compounds including: Tamarixetin, Formononetin, isoliquritigenin, Phloretin, luteolin, Quercitrin, quercetin, myricetin, Wongonin, Puerarin, Genistein, Nordihydroguaiaretic acid, Narigenin, Capillarisin, Chrysin, Fisefin, eriodictyol, 6-Gingerol, Isorhamneti, isoquercitrin, Morin, (+)-Taxifolin, isovitexin, 3-Phenylpropyl Acetate, Oleanolic acid, ursolic acid, β-Myrcene, cinnamic acid, Luteolin-7-Glucoside, Liquiritin, (+)Limonene, Homoorientin, Swertiamarin, Embelin, Daidzein, Poncirin, (−)-Epicatechin, ergosterol. These natural products can be used to enhance the bioavailability of therapeutic agents (drugs).

The invention discloses bacillus velezensis and an application thereof to relieving continuous cropping obstacles of apples. The bacillus velezensis is preserved in China General Microbiological Culture Collection Center on June 10, 2020, and the biological preservation number of the bacillus velezensis is CGMCC NO. 20057. The bacillus velezensis XC1 provided by the invention is a novel biocontrolbacterium having great development potential and has a relatively strong inhibition effect on f. oxysporum, f. moniliforme, f. proliferatum and f. solani, and the inhibition rate of the bacillus velezensis XC1 on the f. oxysporum is the highest and reaches 79.83%. The strain can achieve high yield of antagonistic enzymes such as protease and cellulase; the content of phenolic acid substances suchas phlorizin, phloretin, ferulic acid and cinnamic acid in the soil is reduced; apple tree plant growth is promoted; the method has an excellent effect in relieving continuous cropping obstacles of apple trees, and provides a theoretical basis for biological prevention and treatment of the continuous cropping obstacles of the apple trees.

The invention relates to a purification method of phloretin powder, belonging to the technical field of the chemical industry. The purification method comprises the following steps of: mixing and purifying leechee shells and an alcohol solution to obtain a crude extract; carrying out ion exchange resin separation after concentration; and finally carrying out macroporous resin separation to obtainthe phloretin powder. The invention has the advantages that the process is simple, the extraction ratio achieves above 60%, the purity of the extracted phloretin can achieve above 95%, and the extracted phloretin is applied to the fields of cosmetics.

The invention provides a process for purifying phloretin. The process comprises the following steps of: extracting ripen apple leaves and Chinese flowering crabapple serving as raw materials for 2 to 3 times with ethanol; after extract is concentrated, extracting the extract for 3 to 5 times with water-saturated n-butyl alcohol; gathering and purifying through macroporous resin; eluting with 40 to 70 percent ethanol; concentrating, and preserving heat; performing biological enzymolysis for 15 to 24 hours; concentrating and precipitating; refluxing precipitates with 90 to 99 percent ethanol for crystallization; and drying to obtain a product. In the process, the raw materials are readily available; requirements on equipment are simple; a method is easy to operate; a non-toxic reagent is used; and the obtained product has high quality.

The invention relates to a water-soluble phloretin solid dispersion and a preparation method thereof, and belongs to the technical fields of chemical industry and medicine, in particular to a water-soluble phloretin solid dispersion, which has good water solubility and improved anti-inflammation effect, as well as a preparation method thereof. According to a prescription, the water-soluble phloretin solid dispersion with solubility greatly improved is prepared by dissolving phloretin and a carrier in an organic solvent in the ratio of (10-50) parts to (50-90) parts of the phloretin to a high polymer material. In addition, the invention also inspects the anti-inflammation effect of the water-soluble phloretin solid dispersion. The water-soluble phloretin solid dispersion can develop the anti-inflammation effect of the phloretin more effectively; and the water-soluble phloretin solid dispersion disclosed by the invention is wide in raw material source, good in water solubility, high in stability, good in dispersibility, simple in processing technology and excellent in quality. The solid dispersion is applicable to various fields such as foods, health care products, medicines, cosmetics and the like.

The invention relates to a method for producing phloretin by fermentation of saccharomyces cerevisiae. The method is completed by performing catalysis on p-hydroxyphenylpropionic acid serving as a rawmaterial and the saccharomyces cerevisiae serving as host bacteria through a plurality of enzymes in the host bacteria. Compared with the prior art, the method disclosed by the invention is characterized in that (1) by taking a low-cost compound as the raw material, the technological raw material is low in cost; (2) on the technical basis of modification of microorganisms, catalytic synthesis ofbiological enzymes is carried out in the microorganisms, so that large-scale extraction, separation and purification processes are avoided, and the production cost and the environmental protection areeasy to control; (3) the scale and the quantity of production equipment in the process are less than the scale and the quantity in other methods, and industrial transformation is facilitated; (4) separation and purification procedures are only carried out in the last step of the production in the process, so that a product purification process is simple, the product quality is higher than that ina general method, and the content is higher; (5) no waste liquid is discharged in a synthesis process, so that the method is environmentally friendly and pollution-free and can realize sustainable production.

The invention discloses an HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties. The method comprises the following steps: removing the core of an apple; adding ascorbic acid; preventing browning by virtue of an ultrasound, nitrogen protection or heating method after the core is crushed; adding a methanol-water solution or ethyl acetate for ultrasonic extraction; collecting the supernatant and extracting once again; combining the supernatants; and performing HPLC detection. Chlorogenic acid and rutin are used as target compounds for distinguishing seven varieties of apples according to content analysis of the measured five polyphenols of different varieties and different regions. The method disclosed by the invention has the advantages that the five polyphenols are separated and measured simultaneously and rapidly by virtue of PHLC, and the purpose of distinguishing different apple varieties is achieved; the pre-processing of the method is simple; the cost is low; the measurements can be performed simultaneously; and the distinguishing for different apple varieties is realized.

The invention discloses a method for preparing yellow pigment from phlorizin through two-enzyme process conversion. The method is applicable to preparation of water soluble yellow pigment while phlorizin is taken as a raw material. The method disclosed by the invention comprises the following steps: adding beta-glucosaccharase and polyphenol oxidase into a phlorizin enzymatic oxidation reaction system at the same time, so that part of phlorizin is decomposed into phloretin by the beta-glucosaccharase in advance, and reaction activity is improved; then carrying out enzymatic oxidation under the action of polyphenol oxidase, so that reaction rate and efficiency are greatly improved; and further extracting and purifying, so that the water soluble yellow pigment is obtained. The yellow pigment is bright yellow under the condition that pH is 2-5 and has biological activities such as oxidation resistance. By adopting the method disclosed by the invention for preparing apple yellow pigment, enzymatic conversion rate of phlorizin is effectively improved, and the problem that cost is too high as phloretin is directly adopted as an oxidization substrate raw material is avoided, so that the method disclosed by the invention has a relatively good application prospect.

The invention relates to a technology for semisynthesis of a whitening and anti-skin cancer active ingredient phloretin from natural phlorizin. The technology includes: placing a phlorizin crude product in a stainless steel reaction kettle, dissolving the phlorizin crude product in a 5% strong alkaline solution, conducting simple refining and filtration to obtain a phlorizin salt solution, shifting the phlorizin salt solution into a jacket-equipped enamel reaction kettle, and adding Lewis acid and a metal catalyst at low temperature to let them react for 10-15h at 35DEG C, then lowering the temperature to 0DEG C to perform crystallization, carrying out pumping filtration and deionized water washing to obtain a phloretin coarse grain, then dissolving the coarse grain in a 95% ethanol solution, conducting fine filtration, concentration and cooling crystallization, then performing pumping filtration and drying, thus obtaining a high quality phloretin fine product with content and purity both greater than 99.0%. The technology is suitable for large-scale industrial production.

The invention discloses penicillium purpurescens QL-9204 and application for preparing phloretin during phlorizin conversion. The penicillium purpurescens QL-9204 is a strain free of toxicity and harm and safe to use. The penicillium purpurescens QL-9204 grows rapidly, and is high in hybrid bacteria resistance, easy to culture and stable in batch; a fermentation medium is simple in components and low in market price, thereby being low in production cost; a substrate plorizin is directly put into a crude enzyme for conversion, the enzyme separation and purification steps are omitted, the technology is simple, and the penicillium purpurescens QL-9204 is easy to apply industrially; the phloretin conversion yield is high and can reach 92.5% to the maximum, and the penicillium purpurescens QL-9204 has the advantages that few by-products are produced, and the product is easy to extract.

A method of inhibiting premature aging of human skin caused by exposure to infrared radiation comprising contacting the skin with a composition containing a therapeutically-effective amount of at least one protective compound chosen from baicalin, phloretin and neohesperidin dihydrochalcone, as well as combinations thereof.

The invention provides a saccharomyces cerevisiae. The biological preservation number of the saccharomyces cerevisiae is CCTCC NO M2018718, and a production process of phloretin is obtained through research in combination with the saccharomyces cerevisiae and mainly comprises the processes of activation of a saccharomyces cerevisiae strain in a culture medium, expansion culture, fermentation culture in a fermentation tank, inoculation, material replenishing and fermentation production. The provided production process of the phloretin has the advantages that the cost is low, requirements for equipment are simple, produced reagents are free of toxic substances and harmful substances, and the production process is in line with an environment-friendly production concept; moreover, the yield ofthe phloretin is considerable.

The invention belongs to the technical field of plant extracting, particularly relates to a filamental flowering crab extract comprising filamental flowering crab total extract, 40% of methyl alcohol part extract, 60% of methyl alcohol part extract and methyl alcohol part extract, as well as preparing and application of an active compound baohuoside II (1), kaempferol-3-O-alpha-L-furanoarabinoside (2), phloretin-4'-O-heteroside (3) and kaempferol-3-O-alpha-L-rhamnoside (4) separated from the filamental flowering crab. An experiment proves that each extract and each active compound of the filamental flowering crab have an anticoagulation action, and can be used for preparing anticoagulant singly or in a combining manner.

The invention relates to a simple, convenient and easy preparation method of hydroxytyrosol pellets. The invention comprises a technology and preparation method which avoids the problem of difficult form and bad stability of hydroxytyrosol in prior technology and provides a novel hydroxytyrosol preparation, pellets. The invention adopts a micronization technology of crushing the embedded hydroxytyrosol powder into micro powder, the average grain diameter of the micro powder is in general less than 15um, and the micro powder is mainly distributed in the range of 5-20um, so as to improve the active principle absorptivity and the bioavailability; in addition, the invention can raise the internal dissolvability of the phloretin greatly by using water-soluble adhesive and excipient, so as to improve bioavailability of the preparation and accelerate the initial time to become effective. The invention brought in pellets technology which developed rapidly in medical field, can produce the pellet-type product with controllable release in vivo and high bioavailability so as to carry out the preparation and pave the road for hydroxytyrosol application study.

The invention relates to a chemical phloretin preparing raw material pulverizing device, and discloses a device through which raw materials are subjected to uniform air drying through a spoiler, the raw materials are subjected to water removal and embrittlement, and then grinding and pulverizing are conducted. The chemical phloretin preparing raw material pulverizing device is characterized in that an annular fixing frame is arranged at one end of a body barrel, a ceramic lining is arranged on the inner wall of the body barrel and provided with a spiral groove with the semicircular groove section, and the section radius is gradually increased from the two ends of the spiral groove to the middle portion; a fixing disc is arranged at the center position of the annular fixing frame through asupporting rod, and an annular window is formed by the fixing disc and the annular fixing frame; two cover plates are combined into a ring shape and correspondingly cover the annular window; a fixingsleeve is arranged at the middle portion of the fixing disc and communicates with the interior of the body barrel; an air inlet pipe is arranged on the body barrel and is close to one end of the annular fixing frame; an air outlet pipe is arranged on the body barrel and corresponds to an air inlet hole; and the other end of the body barrel communicates with one end of a grinding barrel, the otherend of the grinding barrel is provided with a discharging port, and the grinding head is in a horn shape.

The invention relates to the field of skin care products, in particular to an anti-inflammatory melatonin redness-calmed repair ice mask and a preparation method thereof. The anti-inflammatory melatonin redness-calmed repair ice mask includes an ice mask cloth and an ice mask essence, and the ice mask essence is prepared form water, glycerinum, 1,3-propylene glycol, dipropylene glycol, allantoin,phloretin, a modified herba lysimachiae extract, a modified asiatic pennywort herb extract, a modified borneolum syntheticum essential oil, a modified flos lonicerae extract, a modified herba portulacae extract, saccharides isomeride, trehalose, phenoxyethanol and an essence; and the modified herba lysimachiae extract, the modified asiatic pennywort herb extract, the modified borneolum syntheticumessential oil, the modified flos lonicerae extract and the modified herba portulacae extract are added to strengthen the skin care effect, sterilization and antiphlogosis are achieved, the allergic skin is improved, facial melanin residues are removed, and the white, rosy and glossy skin is restored.

The invention relates to application of phloretin in preparation of medicine for preventing and treating diabetes. A spontaneous diabetes animal model kkay mouse for transferring Ay genes is introduced, the influence of phloretin on related indexes such as blood glucose and blood fat of diabetes in the mouse is researched, an antidiabetic effect of the phloretin is proved, and a basis is provided for applying the phloretin to the medicine for preventing and treating diabetes.

The invention relates to a method for Escherichia coli whole cell catalyzed production of phloretin. The synthesis method is characterized in that the production of the phloretin is completed by adopting phenylalanine as a raw material and an Escherichia coli engineering bacterium as a host bacterium through catalysis of multiple enzymes in the host bacterium; and the host bacterium at least can express phenylalnine ammonialyase, cinnamic acid-4-hydroxylase, 4-coumaric acid coenzyme A ligase, double-bond reductase and chalcone synthetase. The method has the following advantages: 1, the phenylalanine is used as the raw material, so the raw material cost of the method is low; 2, catalytic synthesis of biological enzymes in microorganisms is carried out based on microorganism modification, solarge-scale extraction, separation and purification processes are avoided, and the production cost and the environmental protection are easy to control; 3, the size and the quantity of production devices used in the method are less than those of other methods, so the method can be easily industrially converted; and 4, only the final step in the production of the method contains separation and purification processes, so the product purifying process is simple, and the product has better quality and higher content than the product obtained through general methods.

The invention relates to a phloretin preparation method, and belongs to the technical field of plant extraction. The method concretely comprises the following steps of (1) poach plant materials with rich phlorizin content with water to obtain water boiling liquid; (2) inoculating green trichoderma spore suspension into the water boiling liquid obtained in the step (1); performing fermentation; after the fermentation is completed, filtering the fermentation liquid to remove thalli; so as to obtain filter liquid; firstly refrigerateing the filter liquid; then, obtaining precipitates from refrigerated filter liquid; next, purifying the precipitates to prepare phloretin solids. The method has the advantages that the operation is simple and convenient; a great number of organic solvents are notneeded; the cost is low; the green and environment-friendly effects are achieved; in addition, the finally prepared phloretin has high yield and high purity; the method is suitable for large-scale production of the phloretin.

The invention discloses an application of phloretin in preparation of a pig, sheep or bovine semen cryopreservation agent. Meanwhile, the invention discloses a cryopreservation agent containing phloretin and a freezing base solution. According to the invention, phloretin is used for cryopreservation of semen of pigs, sheep and cattle for the first time; the application is reliable in effect, easyto popularize and suitable for artificial insemination of pig, sheep and cattle tubule frozen semen, long-term storage of the semen after ultralow-temperature freezing and semen deposition after long-distance transportation, the sperm motility rate, acrosome integrity rate and plasma membrane integrity rate of the frozen-thawed pig, sheep and cattle can be remarkably increased, and the fertilization capacity of sperms is kept.

The invention relates to the field of health food, and discloses a phloretin orally disintegrating tablet and a preparation method thereof. The phloretin orally disintegrating tablet contains the following ingredients by weight percent: 2.5-85% of phloretin solid dispersion, 2-80% of a filler, 2-20% of a disintegrating agent, 0-25% of a bitter taste masking agent, 0.1-7.5% of a lubricating agent and 0-10% of an adhesive, and the total weight of the ingredients is 100%. The invention also discloses a preparation method of the phloretin orally disintegrating tablet. The preparation method comprises the following steps: sieving the phloretin solid dispersion, the filler, the disintegrating agent, the bitter taste masking agent and the adhesive, mixing the ingredients according to an equivalent progressive increase principle, and then adding the lubricating agent, and mixing uniformly; and carrying out a direct powder compression method, and carrying out tabletting by adopting a round die to obtain the phloretin orally disintegrating tablet. The phloretin orally disintegrating tablet disclosed by the invention can be rapidly disintegrated in the mouth so as to be absorbed quickly, is high in biological activity and utilization ratio, and convenient to carry and transport, and can be stored for a long time.

The invention provides a method for improving contents of effective ingredients and antioxidation activity of Docynia indica(Wall.)Dcne by utilizing eurotium cristatum. The method comprises the following steps: a, preparing a Docynia indica(Wall.)Dcne tea crude product; b, taking the Docynia indica(Wall.)Dcne tea crude product obtained in the step a, firstly carrying out steam treatment for 10 minutes, and carrying out moisten heaping treatment at a temperature of 45 to 55 DEG C for 3 to 5 hours; c, fermenting the eurotium cristatum: adding sterile water according to the proportion of a water-to-feed ratio of 20 to 40 percent (mL / g), and inoculating the eurotium cristatum; then performing culture and fermentation at 25 to 35 DEG C for 2 to 60d; d, drying a fermented material obtained in the step c to obtain Docynia indica(Wall.)Dcne fermented tea. The Docynia indica(Wall.)Dcne fermented tea prepared by the method disclosed by the invention has good sensory quality and good taste; moreover, content of amino acid is high, content of phloretin is high, nutrient contents are rich, and application prospect is good.

The invention discloses a skin care and nourishing ice mask. The ice mask is prepared from water, glycerin, 1,3-propanediol, allantoin, carbomer, yeast ferment extract, ferulic acid, Kalanchoe pinnataextract, propylene glycol, essence and other raw materials. The invention further discloses a preparation method of the skin care and nourishing ice mask. The skin care and nourishing ice mask is prepared from glycerin, propylene glycol, allantoin, phloretin, the Kalanchoe pinnata extract, the yeast ferment extract and other raw materials, the preparation method is simple, large equipment is notneeded, and production cost is low; the mask is rich in repair essence, can relieve skin, improve aged cutin and supplement skin collagen and enables the skin to be elastic, moist, tight, smooth and delicate; especially the Kalanchoe pinnata extract as the essence component is added, the skin can be conditioned and improved effectively and nourished and cared intensively, the skin is crystal clearday by day, elastic, moist and tight, silky in texture, smooth and delicate, wonderful skin care effect is shown, and a user is gorgeous.

The invention discloses a method for preparing high-purity phloretin by utilizing shaddock peels. The method comprises the following steps: after crushing dried shaddock peels, extracting through water refluxing to obtain an extracting solution; adding an acid solution into the extracting solution, and carrying out an acid hydrolysis reaction; carrying out a hydrogenation reaction on a hydrolysis reaction product; acidifying and recrystallizing a hydrolysis reaction product in sequence to obtain the product. The method takes the shaddock peels as the direct raw material, so that the cost is low; the method has the advantages of simple process steps, high phloretin yield and high purity, and meets industrial production requirements.