The present invention discloses a preparation method of a biocontrol
bacteria fermentation broth for preventing and controlling
apple tree rot diseases. According to the present invention, two strains such as LF17 and LF18 having a good
apple tree rot
disease bacteria inhibition effect are separated and screened from the
fruit tree branches in the Shanxi Province apple
production area Linfen City apple
orchard, and the two strains are identified as B.subtilis and through strain colony characteristic analysis, strain microscopic characteristic analysis and 16SrDNA
sequence analysis; the biocontrol
bacteria fermentation broth preparation steps comprise: S1, culture medium preparing, S2, antagonistic strain activation, S3, antagonistic strain
liquid culture, S4,
fermentation forth
filtration and concentration, S5, antagonistic bacteria
fermentation broth field prevention and control agent preparation, and S6, antagonistic bacteria
fermentation broth paste field prevention and control agent preparation; and the obtained biocontrol bacteria fermentation forth has characteristics of effective apple rot
disease prevention and control, relative safety to humans and animals, good environmental compatibility,
drug resistance generation resistance, rapid
wound healing, and low scar recurrence rate.