The invention discloses an Illicium henryi-contained anti-virus ointment for external use and a preparation method and application thereof and belongs to the technical field of Chinese herbal medicine processing. The Illicium henryi-contained anti-virus ointment for the external use comprises the following substances in weight percentage: 35-51 parts of Illicium henryi extractive, 2-7 parts of taxifolin, 6-15 parts of borneol, 3-9 parts of propylene glycol, 6-15 parts of beewax, 4-11 parts of glycerol and 8-17 parts of absolute ethyl alcohol. The Illicium henryi-contained anti-virus ointment for the external use has the effects of preventing and curing dermatophytosis, tinea of feet and hands and arthritis, diminishing inflammations and stopping pain, meanwhile, can be used for preventing and curing infections of viruses, bacteria and fungi on body surfaces of fowls, people and livestock and animals and has a specially good effect on preventing and curing swelling and pain caused by the viruses, the bacteria and the fungi. The Illicium henryi-contained anti-virus ointment for the external use is simple and convenient in production method, low in cost, sufficient in raw material, convenient in packaging, storage and transportation and good in using effect.