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The invention discloses flame-retardant, antibacterial and transparent super-amphiphobic paint and a preparation method and application thereof. The flame-retardant, antibacterial and transparent super-amphiphobic paint is prepared by the following steps: firstly preparing inorganic / organic nanoparticles modified by a nitrogen-containing positive ion silane coupling agent, and dispersing the inorganic / organic nanoparticles along with flame retardant particles and a fluorine-containing silane coupling agent in a solvent and reacting. By a spray-coating, brush-coating or dip-coating method, the paint is applied on the surface of a metallic base material, an inorganic base material or an organic polymer base material. The use amount of the coating obtained is less. It can be effectively ensured that characteristics such as color, texture, pores, air permeability, etc. of the surface of the base material are unchanged, and excellent and stable super-amphiphobic property, flame retardant property and antibacterial property can be obtained. Meanwhile, the preparation is simple, the raw materials are cheap and easily available, and no toxic organic solvent is used. The preparation condition is mild, and the preparation is carried out at normal temperature and in an ordinary state and is environmentally-friendly. The preparation cost is low. Thus, the method is suitable for large-scale production and is good for industrial application and promotion.