Preparation and application of boric acid functionalized resin

Abstract

The invention provides preparation of a boric acid functionalized resin and an application of the boric acid functionalized resin to separation and purification of high-purity oleuropein, so as to overcome the defects of high usage amount of a target solvent, complex process, low purity of the oleuropein and low yield in the prior art. A m-aminophenylboronic acid resin is simple in preparation process, simple and convenient to operate and free from corrosive chemical reagents; a process route for purifying the oleuropein is simple, the purity can reach 60% or above, the yield can reach 60% orabove, the cost is low, the efficiency is high, and the method is suitable for large-scale industrial production. The m-aminophenylboronic acid functionalized resin can be regenerated and repeatedly used, and the adsorption capacity is reduced by about 5% after the m-aminophenylboronic acid functionalized resin is recycled for five times.

Description

Technical field [0001] The invention belongs to the technical field of preparation and application of natural product separation materials, and specifically relates to the preparation of a boric acid functionalized resin and its application in the separation and purification of high-purity oleuropein. Background technique [0002] Olive (Oleaeuropaea L.), as an economic crop, was mainly planted in Greece, Italy and other countries along the Mediterranean coast at first, and then introduced to my country. At present, it is mainly cultivated in Yunnan, Sichuan, Gansu, Guizhou and other places. [0003] Olive leaves are the main by-product of the olive planting industry. They are discarded every year when pruning and harvesting fruits. However, olive leaves contain a variety of active substances such as polyphenols, flavonoids, seroid and diflavonoids, etc. The waste produced in the oil production process, such as olive pomace and olive wastewater, is also rich in oleuropein (OL). As...

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Problems solved by technology

[0004] Chinese patent 201010549802.X discloses a method for extracting oleuropein from olive leaves. In this method, the olive leaves are extracted with ethanol, decolorized with activated carbon, extracted with n-butanol, and finally separated by high-speed countercurrent chromatography to obtain a purity of 90%. The above oleuropein, but this method is cumbersome to operate, consumes a lot of solvent, and the yield of oleuropein in the whole process is low, only about 1%, and the production cost is high, which is not conducive to industrialized production; Extracting oleuropein from leaves can produce oleuropein with a content greater than 40%. This method needs to be adsorbed by a macroporous resin and eluted with an alcoholic water solvent, decolorized resin adsorption and organic solvent extraction. The disadvantage is that it needs to go through two resin and One-time extraction, cumbersome operation, will lose a lot of oleuropein, low yield, increase production cost; Chinese patent CN102451235B discloses a preparation method of olive leaf extract, olive leaf is extracted by polar solvent, diatomaceous earth absorbs oil , Macroporous resin adsorption / desorption, the desorption solution is concentrated and dried to obtain olive leaf extract, and the purity of oleuropein can reach more than 55%. This method needs steps such as olive leaf removal, crushing, and water precipitation, and the process is time-consuming

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