379 results about "Ciprofloxacin" patented technology

A powder for inhalatory aerosol delivery, the powder having: spray freeze dried liposome particles with a biologically active agent, such as an antibiotic, encapsulated within a phospholipid, and a method of producing a powder for inhalatory aerosol delivery, the method including the steps of: mixing a biologically active agent with a phospholipid to form a liquid liposome suspension; and spray freeze drying the liposome suspension to form particles of powder.

The invention provides a preparation method of an oxidized graphene and sodium alginate composite absorbing material capable of removing ciprofloxacin in an aqueous solution. The preparation method comprises the following steps: dispersing a certain amount of oxidized graphene samples in deionized water, putting the oxidized graphene samples into an ultrasonic cleaner, and performing ultrasonic treatment for 6-10 hours; dissolving a certain amount of sodium alginate into an oxidized graphene solution subjected to ultrasonic treatment, and performing ultrasonic treatment for 20 minutes; dropewise adding the mixed solution into a 0.2mol / L calcium chloride solution by virtue of a peristaltic pump, soaking for 1-3 days, and cleaning with deionized water for 3-4 times, thereby obtaining a sodium alginate and oxidized graphene sphere hydrogel material; and freeze-drying, thereby obtaining oxidized graphene and sodium alginate sphere aerogel. The preparation method is performed under a relatively mild condition, and oxidized graphene is packaged in a sodium alginate network structure by an ion bridge method, so that the nano toxicity of graphene powder is avoided, and a new nano material capable of efficiently absorbing ciprofloxacin antibiotics in the aqueous solution is prepared. The preparation process is simple, easily controllable in condition, low in cost and suitable for large-scale batch production.

A pharmaceutical composition comprising a fluoroquinolone such as ciprofloxacin, cyclodextrin, and a hydroxy acid is described. The composition may be an aqueous composition, with such aqueous compositions preferably having a pH between 5 and 7. In some preferred embodiments, the composition further comprises a soluble polymer.

The present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. A suitable antibiotic is selected from the group consisting of doxycycline, gentamicin, tobramicin, ciprofloxacin, clindamycin, clarithromycin, azithromycin and metronidazole. Preferred antibiotics are doxycycline and ciprofloxacin. More preferably, the antibiotic is doxycycline. In its second aspect, the present invention is directed to a method for treating periodontal disease in a mammalian patient comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. Typically, the mammalian patient is a human.

The invention describes novel organic nitric oxide donor salts of a antimicrobial compounds, and novel compositions and kits comprising at least one organic nitric oxide donor salt of an antimicrobial compound, and, optionally, at least one nitric oxide enhancing compound and / or at least one therapeutic agent. The invention also provides methods for (a) treating bacterial infections; (b) treating viral infections; (c) treating fungal infections; and (d) treating lesions. In one embodiment the antimicrobial compounds of the invention are aztreonam, ciprofloxacin, doripenam, duramycin and tobramycin. The organic nitric oxide donors that form salts are preferably organic nitrates, organic nitrites, nitrosothiols, thionitrites and heterocyclic nitric oxide donors. The heterocyclic nitric oxide donors are preferably furoxans, sydnonimines, oxatriazole-5-ones and / or oxatriazole-5-imines. The methods of the invention are preferably for the treatment of bacterial infections associated with pulmonary diseases such as cystic fibrosis and for treating Bacillus anthracis infections.

The present invention relates to a method for determining 10 kinds of antibiotics in a water environment through combination of a sample pre-treatment technology and HPLC-MS, and belongs to the field of detection of safety of trace organic contaminant residue in the water environment. The method is characterized in that a water sample is separated and enriched through combination of solid phase extraction and dispersive liquid-liquid microextraction (SPE-DLLME), and then an ultra-high performance liquid chromatography-mass spectrometry instrument (UPLC-MS / MS) is adopted as a detection tool to directly determine the contents of 10 kinds of common antibiotics in the water environment (drinking water, tap water, river water, sewage treatment plant influent and effluent), wherein the 10 kinds of the common antibiotics respectively are sulfadiazine, sulfamethoxazole, oxytetracycline, tetracycline, doxycycline, ciprofloxacin, levofloxacin, chloramphenicol, cefuroxime axetil and tinidazole. According to the present invention, the water sample pre-treatment method and the instrument detection conditions are investigated and optimized, and the optimal SPE-DLLME-UPLC-MS / MS method is established and is successfully applied for the real sample determination; and compared with the traditional method, the method of the present invention has advantages of high sensitivity, high extraction recovery rate, wide application objects, environmental protection, and the like.

The invention relates to a method for measuring 12 types of remaining medicine in a water environment through separation and enrichment at the same time, and belongs to the field of safety detection of a trace of organic pollutant residue in the water environment. The content of 12 types of frequently-used medicine in the water environment (drinking water, faucet water, river water and water discharged into and out of sewage treatment plants) is directly measured with an ultra performance liquid-chromatography-mass spectrometer (UPLC-MS / MS) as a detection tool after a water sample is subjected to solid phase extraction combined with ultrasonic-assisted dispersion liquid-liquid micro-extraction (UA-DLLME) separation and enrichment. The 12 types of antibiotic include ketoprofen, ciprofloxacin, tinidazole, tolfenamic acid, sulfadiazine, sulindac, naproxen, sulfamethoxazole, chloramphenicol, cefuroxime axetil, piroxicam and mefenamic acid. Inspection and optimization are conducted on a sample pretreatment method and instrument detection conditions of the water sample, and the optimal UA-DLLME method is established and is successfully applied to practical sample detection. Compared with a traditional method, the method has the advantages of being high in sensitivity, high in extraction and recycle rate, wide in suitable object, friendly to the environment, and the like.

The invention provides a molecular imprinting catalytic membrane capable of selectively degrading ciprofloxacin and a preparation method. The preparation method comprises the following steps: step 1, preparing graphite-like nitrogen carbonate g-C3N4; step 2, preparing a noble metal loaded semiconductor composite material; and step 3, preparing the molecular imprinting catalytic membrane capable of selectively degrading the ciprofloxacin. The material prepared by the preparation method can effectively realize the aims of preferably selectively adsorbing and degrading target pollutants in a mixed sewage system; post-treatment is simple, a photocatalyst is conveniently recycled for a plurality of times and secondary pollution is effectively avoided; and the molecular imprinting catalytic membrane has the advantage of selectively treating antibiotic wastewater very well.

The invention provides Ciprofloxacin hapten, artificial antigen and antibody and a preparation method and application thereof. Ciprofloxacin is taken as a raw material, and is reacted with aminobutyric or aminocaproic to generate the hapten containing 4 to 6 chiral carbon atoms; the hapten is coupled with protein to prepare the artificial antigen by an active ester method; and the artificial antigen is used to immunize a mouse, and the monoclonal antibody with high specificity on the Ciprofloxacin is prepared by adopting cell fusion technology. The monoclonal antibody is adopted to establish immunological detection for quickly detecting the Ciprofloxacin on site, and has realistic significance for quickly detecting the Ciprofloxacin in livestock, poultry and aquatic products.

The invention discloses manganese-oxidized pseudomonas T34, a method for preparing biogenic manganese oxide and application of the pseudomonas or the biogenic manganese oxide in degrading ciprofloxacin, and discloses a pseudomonas strain pseudomonassp. T34 which is capable of generating a manganese oxide, wherein the strain is preserved with preservation number of CCTCC NO: M2014168. The strain, in an environment with presence of low-valence manganese ions, can be used for oxidizing the low-valence manganese ions in the environment, so that a manganese dioxide compound is formed and the formed manganese dioxide compound is coated on the surfaces of bacteria. The biogenic manganese oxide generated from the strain, which is quite strong in catalytic oxidation capacity, is capable of degrading organic macro-molecular substances into micro-substances which can be absorbed and utilized by the bacteria with efficiency much higher than that of chemically synthesized manganese oxide. The manganese oxide generated from the strain has the advantages of being strong in catalysis capacity, environment-friendly, easy for production and the like, so that a new way is created for solving the current antibiotic pollution status in the environment.

The invention relates to a method for preparing ciprofloxacin by piperazine reaction. The technical problems to be solved are as follows: (1) reducing amount of a solvent or using no solvent, (2) reducing consumption of the piperazine, and (3) shortening the reaction time, simplifying operation, and enhancing overall yield. The method is characterized by (a) mixing the piperazine as shown in formula (iii) with the cyclopropane carboxylic acid as shown formula (ii) at the mol ratio of 1-10:1, adding metal Lewis acid catalyst with the chemical equivalent of 1%-30% for thorough reaction in an aqueous system at the temperature of 80-150 DEG C to obtain reaction solution, wherein, the metal Lewis acid catalyst is selected from one or more of aluminum (III) Lewis acid and iron (III) Lewis acids; (b) adding inorganic base to the reaction solution at the temperature of 20 DEG C-100 DEG C to regulate pH value of the reaction system to 9-12, and filtering while the system is hot; and (c) regulating the pH value of the filtrate to 7-8 with the inorganic base for recrystallization to obtain the product.

The invention discloses a suspension eye drops with ciprofloxacin and anaflogistico to give drug locally, which comprises the following parts: anaflogistico, ciprofloxacin, sodium chloride, hydroxypropylmethylcellulose, polysorbate 80 and acetic acid / sodium acetate buffer, wherein the density of hydroxypropylmethylcellulose is 1. 0-5. 0%. The invention has fitful viscosity to lengthen the retaining time of the drug and improve the biological utility of the drug, which reduces the stimulation for eyes effectively and improves the survivability for the patient.

The invention provides a C / Fe-FeVO4 composite photocatalyst as well as a preparation method and application thereof, and belongs to the technical field of preparation of inorganic nano-materials. The preparation method comprises the following steps: mixing glucose and ferrous gluconate; adding de-ionized water and stirring until a solution is clarified; dissolving substances, putting the solution into a hydrothermal reaction kettle and reacting; after cooling, taking out a product from the reaction kettle; washing with the de-ionized water and absolute ethyl alcohol; drying washed sediment in vacuum to obtain a carbon colloidal ball; adding the carbon colloidal ball into the de-ionized water and carrying out ultrasonic treatment; taking Fe(NO3)3.9H2O, NH4VO3 and an SDBS (Sodium Dodecyl Benzene Sulphonate) surfactant; adding into the carbon colloidal ball and mixing; continually carrying out the ultrasonic treatment; adjusting the pH (Potential of Hydrogen) value of the solution to obtain a grey solution; putting the grey solution into a stainless steel reaction kettle and reacting under a hydrothermal condition; cooling to room temperature to obtain a muddy solution; centrifuging the muddy solution and washing with water and ethanol respectively, and drying to obtain a C / Fe-FeVO4 precursor; roasting the C / Fe-FeVO4 precursor to obtain C / Fe-FeVO4 composed of mesoporous nano-sheet-shaped structures. The C / Fe-FeVO4 composite photocatalyst provided by the invention is used for treating newly-developing micro-pollutants of ciprofloxacin and norfloxacin under visible light.

The invention discloses a method for preparing a selectively degraded ciprofloxacin photocatalyst, and belongs to the technical fields of material preparation and environmental pollution treatment. The method comprises the following steps of: (1) hydrothermal synthesis of a ZnS semiconductor material; (2) ZnS surface modification; (3) preparation of a molecular imprinting polymer photocatalyst; and (4) elution of template modules in the imprinting polymer from the obtained solid powdery substance. The photocatalytic degradation process of the molecular imprinting polymer photocatalyst can effectively fulfill the purposes of selective identification, adsorption and catalytic degradation of target pollutants, the effective degradation efficiency of the target substance is improved, and the photocatalyst has the advantage of strong selective treatment of antibiotic wastewater.

The present invention generally relates to transdermal delivery and, in particular, to transdermal delivery using nanoemulsions and other emulsions. In one aspect, the present invention is directed to emulsions comprising a first, continuous phase and a second, discontinuous phase. The first phase may be an aqueous liquid and the second phase may comprise a lipid, such as isopropyl myristate. In some cases, a surfactant, such as Pluronic® L61, is used to stabilize the emulsion. Surprisingly, it has been found that such emulsions are effective at delivering pharmaceutically active agents, such as ciprofloxacin, when the formulation has a very low water content, for example, less than 30 wt % or less than 10 wt %. This is surprising because high water contents—not low water contents—are typically correlated with greater transdermal drug delivery, and thus, a low water content would have been considered to be unfavorable for facilitating transdermal drug delivery.

The invention discloses a chemiluminescence enzyme-linked immunosorbent test kit of ciprofloxacin, which includes a test kit box body, an enzyme label plate and reagent arranged in the test kit. The test kit is characterized in that holes of the enzyme label plate are coated with envelope antigen which is namely the conjugate of ciprofloxacin and carrier protein; the reagent includes polyclonal antibodies of the ciprofloxacin, enzyme-labeled second antibody which is namely the goat anti rabbit antibody marked by horseradish peroxidase, standard liquid of the ciprofloxacin, concentrated washing liquid and luminescence liquid. With simplicity, celerity and accuracy, the chemiluminescence enzyme-linked immunosorbent test kit has the advantages that the sensitivity is high, and the sensitivity can be enhanced by one order of magnitude compared with traditional colour comparison ELISA; thus playing an important role in ciprofloxacin residue detection of animal food (such as milk, animal tissue and urine samples).

A fluorescence sensitized detection method for ciprofloxacin by utilizing nitrogen-doped carbon quantum dots is disclosed. Soymilk is adopted as a source of carbon and nitrogen, and the nitrogen-doped fluorescence carbon quantum dots are prepared through a one-step hydrothermal process in a high fluorescence quantum yield. The carbon quantum dots can enhance fluorescence of the ciprofloxacin, enhanced fluorescence signal intensity is in a certain linear relation with the ciprofloxacin concentration, and fluorospectrophotometry for detecting the ciprofloxacin is established according to the relation. The prepared carbon quantum dots have significant fluorescence sensibilization effects on the ciprofloxacin, are high in selectivity, and have characteristics of high sensitivity and specificity when the carbon quantum dots are used for fluorimetric determination of the ciprofloxacin.

The invention discloses a quantum dot-based method for detecting ciprofloxacin by immunofluorescence and a special kit. The immunofluorescence detection kit for detecting the ciprofloxacin comprises a ciprofloxacin peridium and a ciprofloxacin antibody marked by a quantum dot. By the method, the quantitative detection of ciprofloxacin residual medicines can be realized, the detection limit is low, the detection sensitivity is high, the specificity is high, and the method is suitable for detecting various samples. Therefore, the method has a bright application prospect in the field of the detection of the ciprofloxacin.

The invention belongs to the field of drugs and pharmaceutics and relates to a pulmonary delivery ciprofloxacin pharmaceutical composition. In particular, a liposome modified by PEG (Polyethylene Glycol) is used as a drug carrier in the composition; the liposome modified by the PEG is that PEG molecules are directly connected to the liposome; or the liposome is coated by the PEG molecules. The invention further relates to the preparation method of the composition. According to the composition disclosed by the invention, the stability is good; the action time is prolonged; the better antibacterial effect is obtained; furthermore, the pulmonary effective treatment concentration can be achieved rapidly; the bioavailability is high; the composition is convenient to carry; and the composition can be taken independently.

The invention discloses a method for preparing an antibacterial medical catheter and aims to provide a method for preparing the antibacterial medical catheter which has the advantages of safety and reliability, high efficiency, simple process, low product cost and so on. The medical catheter with antibacterial function is obtained through preparation of antibacterial powder which is then fully mixed with plastic master batches and subjected to extrusion molding. The method for preparing the antibacterial medical catheter comprises the following steps: firstly, preparation of the antibacterial powder, that is, ciprofloxacin powder and rifampin powder are mixed according to the weight ratio of between 1 to 1 and 1 to 3, and fully mixed with metal tungsten powder; and secondly, preparation of the antibacterial medical catheter, that is, the antibacterial powder and the plastic master batches are fully mixed, and the antibacterial medical catheter is prepared after mixing and extrusion molding.

The invention provides a kind of product that can prevent the contamination in cell cultivating. The characteristic of the product is that every liter product contains 1-50g ethyl ciprofloxacin. The invention adds drug of certain density which can prevent and clear the contamination to the cell culture fluid to prevent the common contamination of bacteria and mycoplasma. The invention has overcome many defects of common drugs such as short drug action period, short shelf life, high price and unable to preserve in ambient temperature. The invention can not only be used in cell cultivating, it can also be used to clarify other organic medium materials, wash the commonly used apparatus or clarify the separating and purifying materials in emergency.

The invention is a kind of immune colloidal gold reagent for measuring the ciprofloaxcin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of ciprofloaxcin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by ciprofloaxcin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

The invention provides a novel flavonoid glycoside and isoquinoline alkaloid complex structure, preparation, antibacterial application and three complex carrier-free nano-drugs with self-assembly performance. The structural general formula of the complex is shown in the specification, the complex has excellent selective antibacterial ability, and the activity of endophyte escherichia coli and probiotics bacillus subtilis and enterococcus faecium is not influenced while pathogenic bacteria staphylococcus aureus is killed; meanwhile, the compound has a good inhibition effect on multiple drug-resistant staphylococcus aureus, and is superior to norfloxacin, oxacillin, tetracycline and ciprofloxacin. The nano-drug formed by self-assembling the three complexes is uniform in form and good in dispersity and stability, gel or fiber is formed under the condition that auxiliary materials are not needed, and support is provided for development of a carrier-free pure drug delivery preparation.

The invention belongs to the technical field of environment material preparation and particularly relates to bamboo stem biochar quantum dot modified Bi4Ti3O12 composite photocatalyst preparation andapplication. A bamboo stem biochar quantum dot modified Bi4Ti3O12 composite photocatalyst preparation method includes: firstly, taking bamboo stem powder as a carbon source to synthesize bamboo stem biochar quantum dot according to a hydrothermal method; secondly, calcining bismuth oxide and P25 in a tube furnace / muffle furnace to obtain a Bi4Ti3O12 material; finally, adding bamboo stem biochar quantum dots and Bi4Ti3O12 into a certain quantity of ethyl alcohol, ultrasonically treating, evaporate to dry ethyl alcohol, and calcining in the tube furnace to obtain a bamboo stem biochar quantum dot modified Bi4Ti3O12 composite photocatalyst. By adoption of bamboo stems as a biochar source for preparing the bamboo stem biochar quantum dots according to a one-step hydrothermal method, compared with a traditional process, the method has advantages of greenness, economy and environmental friendliness; the composite photocatalyst prepared according to the method is capable of effectively makinguse of visible light to degrade ciprofloxacin in antibiotic wastewater and has a promising application prospect in ciprofloxacin wastewater treatment.

The invention relates to a preparation method of a novel adsorbent for selectively separating ciprofloxacin in water environment, and belongs to the technical field of environmental material preparation. The preparation method comprises the following steps: modifying a commercialized dry yeast as a matrix material to form an initiator grafted composite material, and synthesizing a yeast surface molecule imprinting adsorbent in an environmentally-friendly emulsion system by utilizing a surface-initiated atom transfer radical polymerization method with ciprofloxacin as a template, methacrylic acid and hydroxyethyl methacrylate as functional monomers, ethylene glycol dimethacrylate as a cross-linking agent and cuprous bromide as a catalyst. A static state adsorption experiment is used for researching the adsorption balance, the dynamics and the selective identification performance of the prepared blotting adsorbent. A result shows that the yeast surface molecule imprinting adsorbent obtained in the invention has a rapid adsorption kinetics property and a superior identification performance on ciprofloxacin in the water environment.

The invention discloses a detection kit for ciprofloxacin and a detection method thereof, which belong to the technical field of TRFIA and are used for detecting CIP in animal derived food, blood, urine and fodder. The prepared kit adopts the TRFIA to detect the CIP, and the measurement base is labeled immune response. A micro-pore plate is clad with CIP-carrier protein; and CIP standard or sample and CIP antibody are added into the micro-pore plate. The free CIP and the CIP-carrier protein on the micro-pore plate compete for the CIP antibody, the unconnected CIP antibody is removed by washing, then Eu3+-goat anti-rabbit antibody is added into the micro-pore plate, and the unconnected Eu3+-goat anti-rabbit antibody is removed by washing after the labeled immune response. After the enhancement liquid is added, the fluorescence intensity cps is measured by using a time-resolved fluorescence instrument, the fluorescence intensity is in inverse proportion to the concentration of the CIP in the sample, and the content of the CIP in the detected sample can be determined by referring to a stand curve. The kit provided by the invention for detecting the CIP has the advantages of simple structure, using convenience, low price and high sensitivity, and can reach over 0.01ng / mL.

The invention relates to a method for treating antibiotic and heavy metal combined pollution water by using modified graphene oxide. Specifically, nitrilotriacetic acid modified magnetic graphene oxide is taken as an adsorbent and then added to ciprofloxacin and heavy metal ion combined pollution water. An experiment result proves that each gram of the adsorbent contains almost hundreds of milligrams of exchanging ciprofloxacin by mass, the mass of exchanging copper ions reaches more than 60 milligrams, amination modified magnetic graphene oxide has a very good adsorption effect on ciprofloxacin and copper ions, and solids and liquids can be well separated after adsorption. Compared with the prior art, the treatment method is simple, the material preparation and operation cost is low, and antibiotics and heavy metals in the water can be effectively and simultaneously removed.

The invention provides a method for adsorbing and removing TC (tetracycline) and CIP (ciprofloxacin) single and compound polluted water by using a DTPA modified magnetic graphene oxide composite. Theexperiment result shows that the mass of the TC exchanged through each gram of adsorbent in the single antibiotic water is near to hundreds of milligrams, the mass of the exchanged CIP is up to more than 100 milligrams, the adsorbing effects of the DTPA modified magnetic graphene oxide on the TC and the CIP are both quite excellent, and meanwhile, the solid and the liquid can be well separated after the adsorption. The influence of the competitive adsorption on the ciprofloxacin is relatively weak in the compound polluted water, and a new thought is provided for the removal of the ciprofloxacin in the compound polluted water. Compared with the prior art, the treatment method is simple, the material preparation cost and the operation are low, a new way is provided for the treatment of the pollution of antibiotics in the wastewater, and the method can be popularized in the treatment of the antibiotic pollution as a novel adsorbent.