945 results about "Artificial antigen" patented technology

The present invention provides methods of preventing and / or treating cancers (including tumors). In one preferred embodiment, the invention is practiced to induce regression of an existing cancer or tumor and / or to prevent metastasis and / or to prevent growth of metastatic nodules. In other preferred embodiments, the invention may be used as a prophylaxis to prevent the development of primary cancers through a childhood or adult vaccination program against specific tumor antigens for cancers with high incidences. In an alternate preferred embodiment, the present invention provides methods of establishing an immune response against a universal artificial tumor antigen through a childhood or adult vaccine program, thus providing a long-term immune response that can be utilized at any point to treat any cancer which develops later in life. The present invention also provides cancer and tumor cells stably expressing an artificial antigen, preferably an artificial cell-surface antigen.

An artificial antigen presenting cell includes a liposome having at least one recombinant soluble MHC-peptide complex incorporated therein. The artificial antigen presenting cell may also include at least one additional signal molecule incorporated therein for manipulating the intensity and quality of the immune response. The recombinant soluble MHC molecule is obtained by a method utilizing PCR amplification of gDNA or cDNA, and a tag is attached thereto for anchoring the recombinant soluble MHC molecule to the liposome.

The invention provides an in vitro amplification method for efficient and highly cytotoxic natural killer (NK) cells. Novel artificial antigen presenting cells 4-1BBL-mIL-21-aAPC, such as 4-1BBL-mIL-21-K562 cells and the like, are constructed through stably expressing 4-1BB ligands (4-1BBL) and membrane immobilized interleukin 21 (mIL-21) on the surfaces of cell membranes, and by using the novel artificial antigen presenting cells as feeder cells for amplification, the NK cells are directly amplified from peripheral blood lymphocytes. Flow cytometry, cytotoxicity test and the like suggest that the amplified cells NK have high purity and strong cytotoxicity and have obvious killing effect on tumor cells.

The invention relates to a colloidal gold test strip for semi-quantitative detection of progesterone and a preparation method thereof. The invention belongs to the technical field of dairy cow pregnancy diagnosis. Colloidal gold test strip for semi-quantitative detection of progesterone, the sample pad, the gold pad containing gold-labeled progesterone antibody complex, and the nitrocellulose membrane are pasted end to end on the bottom plate; there are detection lines and control lines on the nitrocellulose membrane . Preparation of test strips: preparation of colloidal gold: prepared from chloroauric acid and trisodium citrate; preparation of purified polyclonal antibody: preparation of artificial antigen of dairy cow progesterone by dicyclohexylcarbodiimide coupling method; artificial antigen of progesterone was then used Immune mice to prepare progesterone polyclonal antibody; prepare gold-labeled antibody: colloidal gold and antibody adsorption, centrifugal purification; colloidal gold test strip preparation: soak gold-labeled pad with Tween-containing PB solution, gold-labeled antibody perfusion glass fiber Membrane; nitrocellulose membrane Spray bovine progesterone artificial antigen and goat anti-mouse secondary antibody into 2 lines with a film dispenser. The invention is simple in operation, convenient and practical, efficient and fast, and easy to popularize.

A synthetic method of a general artificial antigen of phthalate plasticizers for immunodetection belongs to the field of bio-chemical engineering technology. The synthetic method of the present invention comprises the following steps of carrying out an esterification reaction between phthalic acid and 6-(fluorenyl methoxy carbonyl acyl-amino)-1-hexanol, removing fluorene methoxy carbonyl acyl protecting groups to form a hapten, and coupling with amino from carrier protein to obtain the general artificial antigen of phthalate plasticizers. Experimental results disclose that titer of antiserum obtained by immunizing animals by the antigen of the invention is up to 160000;the 50% inhibiting concentration IC50 for DBP, DEHP and DINP is less than 500 ng / ml; and the generated antibodies have good generality and high sensitivity. The antigen or antibodies of the invention can be used for the establishment of enzyme-linked immunosorbent assay and colloidal gold test paper rapid detection methods, thus can be used for rapid detection of residues of phthalic acid ester plasticizers in food, and have broad application prospects.

The invention provides an artificial antigen presenting cell as well as a preparation method and an application thereof. Wherein the artificial antigen presenting cell expresses membrane proteins MICA, IL-12 and CD80, the artificial antigen presenting cell is used for culturing NK cells or peripheral blood mononuclear cells containing the NK cells, the number of the NK cells can be remarkably increased, the service life of the NK cells is prolonged, the activity of the NK cells is improved, and compared with an existing artificial antigen presenting cell for expressing MICA, the artificial antigen presenting cell has a stronger effect of amplifying and activating the NK cells.

The invention provides Ciprofloxacin hapten, artificial antigen and antibody and a preparation method and application thereof. Ciprofloxacin is taken as a raw material, and is reacted with aminobutyric or aminocaproic to generate the hapten containing 4 to 6 chiral carbon atoms; the hapten is coupled with protein to prepare the artificial antigen by an active ester method; and the artificial antigen is used to immunize a mouse, and the monoclonal antibody with high specificity on the Ciprofloxacin is prepared by adopting cell fusion technology. The monoclonal antibody is adopted to establish immunological detection for quickly detecting the Ciprofloxacin on site, and has realistic significance for quickly detecting the Ciprofloxacin in livestock, poultry and aquatic products.

A process for preparing artificial antigen, artificial hapten and specific antibody of chlorpyrifos is disclosed. A hapten O,O-diethyl-O[3,5-dichloro-6(2-carboxyethyl) thio-2- pyridyl] thionophosphate (AR) is prepared through reaction between O,O-diethyl-O(3,5,6-trichloro-2-pyridyl) thionophosphate and 3-hydroxypropionic acid. A hapten O-ethyl-O-[3,5,6-trichloron-(2-pyridyl)]-O- (3-carboxypropyl)thionophosphate (PO) is prepared from trichlorothion through 4-step reaction. An artificial antigen is prepared from said hapenjs and protein by coupling. A specific antibody is generated by immunizing animal with said antigen. It can be used to detect the residue of chlorpyrifos.

The invention provides a preparation method and application of a product obtained by coupling penicillin with carrier protein, as well as a beta-lactam type penicillin antibody. Animals are immunized with penicillin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting beta-lactam type penicillin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; beta-lactam type antibiotics coupled with the carrier protein are used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-beta-lactam type antibiotic antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the beta-lactam type antibiotics, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling penicillin with carrier protein, as well as the beta-lactam type antibiotic antibodies can serve the rapid detection of beta-lactam type antibiotic residue in foods.

The invention discloses a synthesis method of a specific salbutamol artificial antigen, belonging to the technical field of biological chemical engineering. The synthesis method disclosed by the invention comprises the following steps of: activating salbutamol through a formaldehyde solution, and connecting 6-aminocaproic acid in the ortho-position of a phenolic hydroxyl group to obtain a salbutamol hapten; and coupling a carboxyl group on the salbutamol hapten with an amino group on a carrier protein to obtain the salbutamol artificial antigen. The synthesis method disclosed by the invention can make up for the insufficiencies and defects of the existing salbutamol antigen synthesis technologies, the salbutamol artificial antigen with high specificity is obtained, the specificity of a produced antibody is high, and the sensitivity is high; and experimental results show that the antiserum titre of an animal immunized by using the salbutamol artificial antigen disclosed by the invention can achieve 80000, the detection limit is 0.5ng / mL, and the half-inhibitory concentration IC50 is 5ng / mL. The antigen or the antibody disclosed by the invention can be used for establishing an enzyme-linked immunosorbent analytical method and a colloidal gold test strip rapid assay method so as to rapidly detect the residues of the salbutamol in a food and further realize broad application prospects.

The invention relates to a analyzing method for hostathion direct competition enzyme immunity absorption analysis2 and its kit. Compose 4 hapten (the chemical formula)(n=1-5) and it covalency coupling symphysis with protein to be artificial antigen, use the artificial antigen immunity animal to prepare for antibody with idiosyncratic affinity to hostathion and use horseradish peroxide to mark the hapten. Use antibody to surround and cover the plate with microholes, add it into the mixture of the sample (or the marked standard sample of hostathion) and hapten marked by enzyme, hostathion, hapten marked by enzyme and the antibody on the surface of the polystyrene micro holes have competitive bond, then clear away the educt, add substrate and color-developing agent of enzyme the intensity of enzymatic colour reaction is inversely proportional to the content of hostathion in the sample (or the standard sample), in accordance with it, the hosathion direct competition enzyme immunity absorption analysis technique is invented. The technology can be applied to choose relative reagent and material in the kit, for preparing kit of immunity test, and rapid test for the polystyrene remained in agricultural products and environment.

The invention relates to a preparation method of a benzothiostrobin antigen and antibody and an application thereof, belonging to the technical field of immunochemical analysis. The chemical name of benzothiostrobin is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]methyl phenylacetate. Under an alkaline condition, carboxylic ester in the benzothiostrobin structure is hydrolyzed to synthesize an artificial hapten of which the chemical name is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]phenylacetic acid; the artificial hapten is coupled with bovine serum albumin and ovalbumin respectively to prepared an artificial antigen. The BALB / c female mouse is immunized by the artificial antigen to obtain a specific monoclonal antibody of benzothiostrobin. The antibody does not experience a cross reaction with other compounds. An enzyme linked immunosorbent assay method established by use of the antibody can be applied to quick, sensitive, convenient and cheap detection of benzothiostrobin residues in environment and agricultural products.