Method for in vitro induction of specific cytotoxic T lymphocytes of hepatitis B virus (HBV)
A hepatitis B virus, lymphocyte technology, applied in the field of cell biology and immunology of biotechnology, can solve problems such as the inability to completely remove the virus
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[0028] 1) Preparation of artificial antigen-presenting cell aAPC that can load any polypeptide:
[0029] Use MHC I-Ig and anti-CD28 mAb to coat magnetic beads: MHC I-Ig and anti-CD28 mAb are coated on magnetic beads (Dynabeads M-450, purchased from invitrogen) by direct coating method, and constructed to load any polypeptide artificial antigen-presenting cell aAPC;
[0030] Specific method: sterilized 0.1M borate buffer washes magnetic beads twice, makes magnetic beads mixed with 1:1 HLA-A2-Ig and anti-CD28 mAb9.3 (monoclonal antibody against CD28, purchased) Incubate at 4°C for 24h on a rotator. Wash twice with bead washing buffer, and then incubate for 24h in buffer containing magnetic beads, then remove the washing solution and add fresh buffer. The prepared aAPC finally contains 0.9×10 per magnetic bead 5 Molecular HLA-A2-Ig and 1.9 x 10 5 anti-CD28 mAb 9.3 molecules. The prepared magnetic beads can be stored at 4°C for 3 months. The coated magnetic beads are aAPC. ...
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