748 results about "Rapid assay" patented technology

The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

The present invention provides novel compositions comprising Epstein-Barr virus-specific oligonucleotides that are useful as primers to amplify particular regions of the genome during enzymatic nucleic acid amplification. The invention also provides a rapid, sensitive and specific method for the detection and quantitation of the virus which may be present in a clinical specimen, using the virus-specific primers and enzymatic nucleic acid amplification; hybridization of amplified target sequences, if present, with one or more Epstein-Barr virus-specific oligonucleotide probes which are labeled with a detectable moiety; and detection of the detectable moiety of labeled oligonucleotide probe hybridized to amplified target sequences of Epstein-Barr virus DNA.

The invention provides a flow velocity monitoring implementation method based on an adversarial generative network. The flow velocity monitoring implementation method comprises the following steps that (1) water flow image preprocessing is performed; (2) image classification is performed based on the adversarial generative network; (3) flow velocity determination: the image classification results and flow velocity intervals are corresponding in a one-to-one way; and (4) state analysis: a state abnormal signal is transmitted when the monitoring result indicates that the flow velocity exceeds the preset threshold. The beneficial effects mainly reside in that the advantages of discriminant and generative classification algorithms are effectively combined in adversarial training of a generator and a discriminator and unsupervised learning is realized, and the synthetic water flow image outputted by the generator of the adversarial generative network and the real image act as the input of the discriminator together so that the robustness of a classifier for the noised water flow image can be greatly enhanced, classification is performed according to the water flow image and rapid flow velocity determination can be realized in a way of being corresponding to the preset flow velocity intervals and classified management of mass water flow information is facilitated.

The present invention relates to a method for analyzing and rapidly determining the quality of a test RNA of unknown quality utilizing a novel quantitative reverse transcription-polymerase chain reaction (RT-PCR) based method. The present invention is based on normalizing the 3′ end of the house-keeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to the relative abundance of the 5′ end of GAPDH MRNA in the test RNA. The present invention is particularly useful in pre-screening postmortem tissue samples for microarray experiments and for evaluating large quantities of samples which would be time consuming and expensive to analyze by methods currently in use.

An aquatic animal morphological character damage-free rapid measuring method belongs to the technical field of aquatic animal measuring methods, which is characterized in that the method comprises the following steps: a standard graduated scale is laid beside the aquatic animal to be measured and a clear image is shot with a digital camera; the image is led into a computer image processing software to determine the ratio X between the unit length of the standard graduated scale and the correspondent image distance; correspondent frame measuring fixed points are selected on the image profile and the image distance L1 between the points measured is measured according to the measuring requirements of aquatic animal morphological characters of different types when the image size is kept unchangeable; the actual distance L0 between the points measured is calculated according to the computing formula that L0 is equal to X*L1. The measuring method realizes the rapid, accurate and damage-free measurement of the shapes of living aquatic animals, which has important meaning for improving the work efficiency of object aquatic animal morphological research and provides possibility for the measurement and tracing observation of the aquatic animal living body.

A method and apparatus are disclosed for predicting the service life of a metallic structure subjected to cyclic loading. Such structures experience fatigue, which can lead to failure after a number of loading cycles. The disclosed invention allows for an accurate prediction of the number of cycles to failure for a metallic structure by observing the slope of the rise in surface temperature of the structure after the cyclic loading has begun. The method of this invention provides early and accurate predictions of service life and does not require destructive testing. The method and apparatus of the present invention may be installed on working equipment, thus providing service life predictions for materials in real world use. The invention uses an empirically derived relationship that was confirmed using analytical relationships and material properties. The derived formula uses two constants that may be determined empirically using a disclosed process. The constants also may be estimated mathematically. The apparatus may include a wireless temperature sensor mounted on the metallic structure of interest and a data analysis unit to perform the needed calculations.

The invention relates to the technical field of quality detection and provides a rapid determination method for the evaluation of the storage quality of grain, which comprises: S1: grain samples having different storage years are acquired according to the grain samples to be evaluated, thus obtaining a modeling sample set; S2: a near-infrared spectrum of the modeling sample set is obtained by means of acquisition and is pretreated; S3: a fatty acid value of the modeling sample set is obtained by means of measurement; S4: a correction relation for the modeling sample set is established according to the near-infrared spectrum and the fatty acid value, thus obtaining a correction model; and S5: the storage quality of the grain samples to be evaluated is evaluated according to the correction model. On the basis of near-infrared spectrum technique, the rapid determination method is in no need of the sample pretreatment, fast in detection, great in convenience and simplicity and suitable forthe determination of the fatty acid value in kernel of grain crop such as paddy rice, wheat, corn, soybean and the like, thus technically supporting the real-time detection and surveillance of the storage quality of grain.

The invention discloses a method for determining the content of zinc in the zinc concentrate through energy-dispersive X-ray fluorescence spectrometry. The method comprises the following steps: 1, preparing a plurality of zinc-iron working liquids, converting the mass concentrations of Zn in the operating liquids to Zn mass percentage concentrations, detecting the Zn mass percentage concentrations with an energy-dispersive X-ray fluorescence spectrometer as a detector, recording the fluorescence intensity during each time injection of a sample, and making a working curve; and 2, processing the zinc concentrate as a sample to prepare a sample solution, injecting the sample solution under detection conditions for making the working curve, recording the fluorescence intensity displayed on the detector, reading out the Zn mass percentage concentration corresponded to the fluorescence intensity according to the determined working curve, and calculating according to a specific formula to obtain the mass percentage concentration of simple substance zinc in the sample. The method utilizes a solution process to eliminate or basically eliminate the matrix effect, so the zinc content can be accurately determined; and the method has the advantages of simple operation, short detection period, good detection result accuracy and high precision.

The invention relates to a rolling circle amplification-based colorimetric assay method for target nucleic acids or proteins. The method mainly utilizes the hybridization between nucleic acids and antigen-antibody binding to indirectly fix the target nucleic acids or proteins to be assayed onto magnetic beads, rolling circles are added, a rolling circle primer on hybrid is utilized to carry out constant-temperature rolling circle amplification, and finally, the chromogenic reaction of nanogold is utilized to assay constant-temperature amplified macromolecular product in order to achieve the purpose of relatively assaying the target nucleic acids and proteins. By integrating rolling circle amplification and the chromogenic reaction of nanogold, the method not only amplifies a large quantity of micro-sample signals, but also realizes the rapid, visual assay of results, avoids the usage of a PCR (Polymerase Chain Reaction) instrument and other expensive instruments, and decreases the requirement on the experimental hardware condition. The method is expected to be applied in bedside rapid diagnosis and the field rapid assay of microorganisms in food safety and the environment.

The invention is a method of determining all the components of an absolute permeability tensor of a porous medium sample from permeability measurements, obtained for example by placing the sample in a permeameter which is useful for fast determination of permeability anisotropies of rocks and detention of internal heterogeneities. A pressure difference ΔP is applied between the inlet and outlet faces of a laboratory rock sample, with zero flow conditions on the edges parallel to the mean flow obtained by confining it in a sheath under pressure. Starting from conventional permeability measurements in three directions and from the measurements of the two components of the viscous forces transverse to the sample, permeability tensor k can be “inverted” by numerical solution of the corresponding boundary-value problem. Since these quantities can be alternatively obtained from numerical solutions of the same flow in a heterogeneous medium, the method can also be used as an upscaling tool in a reservoir simulator.

The invention relates to methods of reliably and quantitatively determining the amount of an analyte of interest in a fluid sample using a flow-induced assay, such as an immunochromatographic assay, in which spatiotemporal measurements are recorded during the course of the assay reaction, generating a spatiotemporal dataset, and subsequently analyzed. The invention also relates to a system incorporating instruments for recording spatiotemporal datasets (spatiotemporal data recorders), devices comprised of flow-induced assays configured for analysis on a spatiotemporal recorder, and programs for analyzing the recorded spatiotemporal datasets.

Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and / or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.

The invention discloses a method that is used for quickly measuring the content of stibium, barium, arsenic, zinc, strontium and zirconium in a TFT substrate glass, realizes measurement on the basis of the inductively coupled plasma atomic emission spectrometry, comprises the steps of experimental condition preparation, sample processing, working curve plotting, interfering and eliminating, and building of the linear relation condition, the detection limit condition and the accuracy condition of the method, wherein, the experimental condition preparation comprises instrument selection, acidity selection and analyzing spectral line selection; the sample processing comprises test sample processing, blank test fluid processing and the measurement, the working curve plotting comprises configuration of a solution in the standard series and the plotting of the working curve; the interfering and eliminating comprises chemical, physical and spectral interfering, background removing and building of the related coefficient between each measured element and the linear relation between the working curves; the blank sample is continuously measured for 10 times, the detection limit of the method is obtained according to a formula, the sample of the same TFT substrate glass is repeatedly measured for 10 times, the precision experiment is implemented, and finally the labeled recovery experiment of the sample is implemented.

Linear arrays of chemosensors or chemical compounds are supported by an optical fiber that allows one to rapidly assay the entire array using changes in optical properties such as fluorescence. The location of the agent along the fiber determines the identity of the agent in these linear arrays. Combinatorial libraries may be constructed on the fiber as well as assayed on the optical fiber. A system and method of analyzing the entire array of agents on an optical fiber using a light source, an optical fiber, and a detector are also described. The time delay between the excitation and detection determines the location being assayed along the fiber and therefore the identity of the agent being assayed. The present invention may find uses in the medical, pharmaceutical, environmental, defense, and food industries.

The invention discloses a quick measuring method of BOD in hyperhaline industrial waste water. The method comprises the following steps: taking 10g sludge from a hyperhaline waste water sewage outfall; adding 90mL sterilized normal saline into the sludge; carrying out streaking on a beef extract peptone culture medium plate for 2d at a temperature of 37 DEG C till obtaining pure salt-tolerant bacterial strain; inoculating the bacterial strain in beef extract peptone culture medium to carry out shaking culture inside a constant temperature shaking box for 24h, centrifugation for 5min at a rotating speed of 4,500r / min and washing by means of 0.9 percent NaCl solution, thereby obtaining salt-tolerant bacteria; dispersing 5mg carbon nano tube in water to form 5mg.mL carbon nano tube suspension; preparing the salt-tolerant bacteria into 0.2 g.mL bacterial suspension by phosphate buffer solution; mixing the bacterial suspension with the carbon nano tube suspension according to the volume ratio of 1:2, and evenly coating 30 mu L mixed suspension on cellulose film with the aperture equal to 0.2 mu; cooling the cellulose film semidry and fixing the film on a dissolved oxygen electrode to form a BOD electrode; and setting a dissolved oxygen analyzer as automatic measuring and recording output current in time by magnetic data acquisition software. The quick measuring method has the advantages of simplicity and quickness, high accuracy and ideal stability.

The invention discloses a method for determining a radix notoginseng extract and contents of five types of ginsenosides in a preparation of the radix notoginseng extract by a Fourier transform near-infrared spectrograph. The method comprises the steps that a sample is detected by an ultra-high performance liquid chromatography, moving phase builds acetonitrile and water gradient elution parameters with separation degrees higher than 1.7 in chromatographic peaks of Rg1 and Re, and the spectrum preprocessing adopts the combination of 2 to 3 methods in Savitzky-Golay polynomial smoothing, second-order differential, Noriis derivative filtering and data normalization; and three preferred wave bands of the five types of ginsenosides are modeled, and a calibration model is built by any one of a partial least squares regression method, a principal component regression method and a multiple linear regression method. The method is used for determining the contents of the five types of ginsenosides in the sample to be detected, the determining result is consistent with a result determined by the ultra-high performance liquid chromatograph basically, the requirements of 'Chinese pharmacopoeia' are met, the accuracy is high, and the determination speed is increased substantially.

The invention discloses a new lubrication oil quality spectrum rapid determination method, which comprises the following steps: (1) collecting representative lubrication oil samples as a training set; (2) determining the infrared spectrum of the training set sample; (3) selecting an appropriate variable optimum technique to preferably select appropriate characteristic wavelengths and establishinglinear regression equation between each quality index and the characteristic wavelength by taking the absorbance of the characteristic wavelength as a variable; and (4) firstly determining the infrared spectrum of an unknown lubrication oil sample for quality test, selecting the absorbance of the same characteristic wavelength and determining each quality index of the lubrication oil by the established linear regression equation. The method can rapidly determine the chemical composition content, physical and chemical properties and 21 quality parameters of major elements and additive elements, the analysis speed is high, the operation is simple, and the quality monitoring capability of the lubrication oil is improved.

The invention discloses a testing piece for quickly testing microorganisms, as well as a preparation method and application thereof. The testing piece comprises a soleplate, a film with a hollow part in the middle and a plastic film. The bottom surface of the film with the hollow part in the middle is adhered to the soleplate; a groove culture area is formed at the hollow part in the middle of the film; a color development culture medium is arranged in the groove culture area; a cold water condensable gellant is adhered to the bottom surface of the plastic film; and the bottom surface of the plastic film and the upper surface of the film are adhered to the edge at one side. After the upper and the lower layers of the testing piece are adhered, irradiation and sterilization packages are used for standby. The testing piece can be applied to the detection of total colony count, coliform bacteria, coliform group, salmonella, staphylococcus aureus, subsidiary haemolytic vibrio, pseudomonas aeruginosa and other food-borne causative organisms and has the advantages of simple operation, convenient carrying, short testing time, accurate result and double-sided bacteria count.

The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.

The present invention proves instruments and methods for detecting and / or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4+T cells in a whole blood sample.

A disclosed rapid determination method for multiple component contents in mold flux comprises the following steps: 1) carbon-removing pretreatment on mold flux; 2) sample dissolving by microwave digestion; 3) preparation of standard solutions of to-be detected components; 4) drafting of standard curves; and 5) determination on contents of the various components in mole flux. By combining microwave digestion sample dissolving technology and inductively coupled plasma emission spectrometry, the multiple component contents in mold flux are determined by one time; the rapid determination method has the advantages of being complete and rapid in reaction and low in blank value; the use amount of an agent is reduced by 50% or more; because microwave digestion is performed under the enclosed condition, the loss of to-be determined elements is reduced and laboratory pollution is reduced; the temperature is strictly controlled in the process of digestion, so that the accuracy of determination results is guaranteed; and the determination result is accurate and reliable, the operation is simple, the analysis speed is improved by 50% or more, the determination period is substantially shortened, and the rapid determination method satisfies comprehensive utilization of mold flux and practical operational requirements in production field.