The invention discloses a method for evaluating antimicrobial susceptibility of drugs. The method utilizes compounds represented by general formulas (I) and (II) to assist to give quantitative susceptibility data of antibiotics against pathogens. The method has the advantages of being sensitive (as low as 2*104 c f u), quick (4-5hours), accurate, visible and the like. Besides, the method is applicable to high throughput screening of antibiotics. Compared with various systems with advanced technologies, the method needs not complicated instruments and equipment, sample treatment is simple, cost is low, outside non-specific effects are few, and antibiotic concentration in inhibition percentage can be optionally provided. In clinic, the method can be used for quick evaluation of antimicrobial susceptibility to instruct doctors to perform drug administration on patients more pertinently, thereby not only treatment efficiency is improved, but also drug resistance caused by incorrect use of the antibiotics is lowered. Meanwhile, the method for evaluating antimicrobial susceptibility of drugs can also have profound effects in terms of food safety, environment detecting, and the like. Formula I and formula II are as followed.