Kit for quickly detecting drug resistance gene of pneumophila pathogenic bacteria

Abstract

The invention relates to a kit for quickly detecting a drug resistance gene of pneumophila pathogenic bacteria, which comprises a gene chip capable of detecting 24 main drug resistance genes of pneumophila pathogenic bacteria and a reaction system for multiple asymmetric PCR reactions. The 24 drug resistance genes are derived from aminoglycosides, quinolones, extended spectrum beta-lactamases (ESBLs), cephalosporins (AmpC), carbapenems and drug resistance genes with vancomycin resistance and methicillin resistance causing membrane permeability transition. The kit can be used for directly detecting drug resistance genes of pneumophila pathogenic bacteria in clinical samples, is simple and quick to operate, does not need culture or drug sensitive tests, gains valuable time for reasonable application of antibiotics to a great extent, and is worthy of clinical popularization and application.

Description

technical field [0001] The invention relates to gene chip technology, in particular to a kit for rapid detection of drug-resistant genes of pneumonia pathogenic bacteria. Background technique [0002] In recent years, due to the aggravation of air pollution and the occurrence of public health incidents, respiratory diseases have attracted more and more attention. Pneumonia refers to the inflammation of lung parenchyma including terminal airways, alveoli and interstitium, and is the leading cause of death from infectious diseases worldwide [1] . According to the World Health Organization (WHO), about 3.5 million people die from lower respiratory tract infection every year, ranking third among all causes of death. [2] . Pneumonia and influenza are the ninth leading cause of death in the United States, with more than 50,000 deaths from pneumonia in both 2010 and 2011 [3-5] , and the statistics did not include the cases of sepsis caused by pneumonia and cancer and Parkinson'...

AI Technical Summary

Problems solved by technology

The drug susceptibility test also has an automated operating system, which is a discontinuous dilution method, which reduces the burden on the operator, but the automated drug sensitivity analysis system cannot flexibly select the type of antibiotics, and still cannot completely replace the K-B method

Infectious Diseases Society of America (IDSA) guidelines once recommended giving antibiotics to patients with lung infections within 8 hours, which was shortened to 4 hours in 2003 [19,20] , it can be seen that the current drug sensitivity test cannot meet the needs of rapid clinical diagnosis and timely targeted treatment measures. The early treatment of pneumonia can only rely on empirical treatment, which further aggravates the emergence of drug-resistant strains. Therefore, there is an urgent need for a clinical Method for accurate and rapid detection of drug-resistant characteristics of pneumonia-causing bacteria

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