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Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method

A technology for the quantitative determination of Helicobacter pylori, which is applied in the field of rapid screening methods and kits for anti-Helicobacter pylori sensitive drugs, can solve the problems of high injury and pain for patients, can only be qualitatively determined, and is expensive, and achieve cheap reagents and methods Simple, cost-saving effect

Inactive Publication Date: 2014-04-30
SICHUAN VACCINE TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Based on the current detection situation, the present invention provides a quantitative detection method using live Helicobacter pylori, which uses live Helicobacter pylori as a detection sample, utilizes the characteristic that live Helicobacter pylori can produce urease, and combines the standard The curve can quickly and accurately read the number of Helicobacter pylori without destroying the Helicobacter pylori. Injury and pain, time-consuming and labor-intensive issues

Method used

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  • Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method
  • Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method
  • Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method

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Embodiment 1

[0027] Quantitative determination method of viable bacteria of Helicobacter pylori

[0028] 8-10 parts of wt2% urea, 0.1-0.5 parts of wt 0.04% phenol red, wt 0.04% NaH 2 PO 4 ﹒ h 2 O 3-5 parts, wt 0.1%Na 2 HPO 4 2-3 parts are configured as sterile urease reagent.

[0029] Draw the standard curve of the CFU / ml value corresponding to the OD value, that is, the number of individual bacteria in each ml of bacterial solution. The standard curve of the CFU corresponding to the OD value is determined by the gold standard of bacteriological detection (colony plate count), and the specific operation of the colony plate count is briefly described as follows:

[0030] Dilute a certain concentration of bacterial solution by a 10-fold gradient, take 100 microliters of the diluted bacterial solution and spread it on the medium, parallel 3 plates, count the number of colonies after 72 hours of cultivation, and dilute the gradient until there are only 1-5 colonies on the plate

[00...

Embodiment 2

[0044] Helicobacter pylori drug susceptibility testing method

[0045] 8-10 parts of wt2% urea, 0.1-0.5 parts of wt 0.04% phenol red, wt 0.04% NaH 2 PO 4 ﹒ h 2 O 3-5 parts, wt 0.1%Na 2 HPO 4 2-3 parts are configured as sterile urease reagent.

[0046] Draw the standard curve of the CFU / ml value corresponding to the OD value, that is, the number of individual bacteria in each ml of bacterial solution.

[0047] The standard curve of the CFU corresponding to the OD value is determined by the gold standard of bacteriological detection (colony plate count), and the specific operation of the colony plate count is briefly described as follows:

[0048] Dilute a certain concentration of bacterial solution by a 10-fold gradient, and take 100 microliters of the diluted bacterial solution and smear it on the culture plate.

[0049] On the base, parallel 3 plates, count the number of colonies after 72 hours of culture, and dilute the gradient until there are only 1-5 colonies on...

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Abstract

The invention discloses quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and a determination method. The method adopts viable helicobacter pylori as a sample to be detected; the property that the viable helicobacter pylori can generate urease is used for rapidly and accurately reading the quantity of the helicobacter pylori by a helicobacter pylori colony counting standard method (CFU / ml, namely the bacterium individual quantity in each ml of bacterium liquid and a colony quantity standard curve); the detection result is accurate and sensitive and has high reliability; the counting difficulty of existing helicobacter pylori clinic microbiological identification, caused by difficult culture and complicated operation, is solved; the drug allergy determination kit and a preparation method, which are expanded by the invention, can be used for carrying out various drug allergy tests at the same time; clinicians can rapidly and conveniently screen suitable anti-helicobacter pylori medicines so that the time and the labor are saved and the cost is saved, so as to provide a beneficial technical solution for clinical scientific treatment.

Description

technical field [0001] The present invention relates to a measurement method of Helicobacter pylori and a preparation method thereof, in particular to a simple, fast and sensitive quantitative measurement method of Helicobacter pylori, a kit thereof and an improved anti-Helicobacter pylori sensitive drug Rapid screening method and its kit. Background technique [0002] 1983 Helicobacter pylori ( Helicobacter pylori Hp for short) was discovered by Warren and Warshail in Australia and has been identified as one of the most common pathogens of chronic infectious diseases. At present, the clinical detection of Hp is mainly divided into two categories: invasive and non-invasive. The former includes gastroscopy and serum antibody detection; the latter includes "C-UBT" detection and Helicobacter pylori antigen detection, but both are inconvenient to detect , The detection has some shortcomings such as trauma and detection hysteresis. [0003] There are many inspection methods f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/18
Inventor 祝洁周永君张勇
Owner SICHUAN VACCINE TECH
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