218 results about "Anti-Helicobacter pylori IgG" patented technology

The invention relates to lactobacillus CN2018, CGMCC No. 4286 and application thereof. The lactobacillus CN2018 has acid resistance, has an effect of suppressing growth of helicobacter pylori (H. pylori SS1 for instance) and urease activity, has a stronger effect of suppressing the adherence of helicobacter pylori to human gastric epithelial cells SGC7901, and has an effect of preventing or relieving infection degree of mice infection of the helicobacter pylori.

An anti-Helicobacter pylori agent comprising a compound represented by the formula:wherein A represents an aromatic ring group which may be substituted; R1 and R2, whether identical or not, each represent a hydrogen atom or a hydrocarbon group which may be substituted; R3 and R4, whether identical or not, each represent a hydrogen atom, a hydrocarbon group which may be substituted, an acyl group, a carbamoyl group which may be substituted, or a carboxyl group which may be esterified; or a salt thereof.

The invention provides lactobacillus plantarum capable of resisting helicobacter pylori infection and application of the lactobacillus plantarum. The lactobacillus plantarum capable of resisting helicobacter pylori infection is named as lactobacillus plantarum Lp05 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.23547, and the preservation date is October 9, 2021. The invention also provides a culture method of the lactobacillus plantarum for resisting helicobacter pylori infection and a product for inhibiting helicobacter pylori infection. The lactobacillus plantarum for resisting helicobacter pylori infection is good in gastric acid resistance, proliferation of helicobacter pylori is inhibited, the adhesion effect of helicobacter pylori on gastric epithelial cells is reduced, and conditions are created for eradicating helicobacter pylori. The product for inhibiting helicobacter pylori infection does not cause adverse reaction and drug resistance of pathogenic bacteria, and is high in application value.

The invention discloses an urea enzyme epitope fuse peptiolipid plastid vaccine to against pylorus bolt bacteria infection, which is characterized by the following: choosing fuse peptide of pylorus bolt bacteria urea enzyme B subunit and stick film adjuvant cholera morbus toxin B subunit as immunogen; coating the immunogen with liposome; producing the liposome vaccine. This liposome vaccine can evoke organism to generate special immune response and inhibit planting of pylorus bolt bacteria in stomach.

The present invention relates to flavanoid compounds of general formula (X1) wherein: R1 is selected from a group consisting of morpholinyl, N-methyl piperizinyl, piperidinyl and N,N′-dimethylamino groups, and n ranges from 3 to 6, and process for preparation thereof. The present invention relates to the demonstration of anti Helicobacter pylori activity and gastric antisecretory activity of semisynthetically designed flavonoid compounds, to be used for the prevention and treatment of gastroduodenal disorders in general and peptic ulcer diseases in particular. The present invention also relates to a hetero-dimeric bi-functional molecule that can be used as monotherapy substituting / replacing / overcoming currently used triple / quadruple therapy, thereby implicating / anticipating / envisaging its commercial applicability.

The invention relates to a helicobacter pylori urease antibody IgM-IgG combination rapid test device and a preparation method thereof, and belongs to the field of medical test equipment. The device is prepared by pasting solid-phase purified high-specificity anti-human IgM and IgG antibodies, a nitrocellulose film of a helicobacter pylori urease antibody, a glass fiber film adsorbing a colloidal gold labeled helicobacter pylori urease antigen, a sample pad, absorbent paper and other auxiliary materials together; a polylysine treatment solution is adopted for pretreatment of the nitrocellulose film; the anti-human IgM and IgG antibodies are combined with silicon dioxide nano-particles at first, and then the combination is adsorbed onto the pretreated nitrocellulose film; an appropriate gold sputtering buffer solution and sample pad treatment solution are prepared; therefore, on the basis that complete release of immune colloid gold is guaranteed, the reaction sensitivity is effectively improved, and the consumption of the immune colloid gold and the cost can be reduced under the same threshold condition; the device is high in test paper sensitivity, strong in specificity, simple and convenient to operate, lower in time consumption, and strong in practicability.

The invention provides a composition for resisting helicobacter pylori and an application of the composition. Each 100g of the composition comprises the following active components according to the content: 5-20g of inactivated lactobacillus reuteri, 0.1-1g of inactivated lactobacillus paracasei, 1.5-25g of bifidobacterium animalis viable bacteria, 1.2-20g of lactobacillus rhamnosus, 5-10g of hippophae rhamnoides fruit powder, 0.1-1g of a broccoli seed aqueous extract, 20-30g of fructo-oligosaccharide and 10-70g of lacitiol. According to the composition, the inactivated lactobacillus reuteri,the inactivated lactobacillus paracasei, the hippophae rhamnoides fruit powder, the broccoli seed aqueous extract, the fructo-oligosaccharide and the lacitiol are prepared into a specific compositionformula together, and the formula not only can well prevent and treat bacterial infection of gastrointestinal tracts and bacterial infection of oral cavities, particularly concurrently has better mouth feel flavor, and is green and healthy due to no addition of seasonings of essence and the like.

The invention discloses a helicobacter pylori urease B antigenic epitope polypeptide and application thereof. The amino acid sequence of the antigenic epitope polypeptide is the sequence 37 in a sequence table, and the encoding gene sequence of the antigenic epitope polypeptide is the sequence 28 in the sequence table. The antigenic epitope polypeptide can stimulate organisms to generate helicobacter pylori resistant protective immune response so as to enhance the suppression of the helicobacter pylori infection, improve the capacity of removing germs of the organisms, and play a promoting role in development of corresponding vaccines, research on pathogenic mechanism, and clinical diagnosis.

The invention provides a kit, application of the kit in detection of pepsinogen I, pepsinogen II, a helicobacter pylori antibody and gastrin 17 and a method for detecting the pepsinogen I, the pepsinogen II, the helicobacter pylori antibody and the gastrin 17 by using the kit. The kit comprises a first coating film and a second coating film, wherein at least one region in the first coating film is coated with a fluorescent microsphere-labeled pepsinogen I antibody, a fluorescent microsphere-labeled pepsinogen II antibody, a first fluorescent microsphere-labeled helicobacter pylori antigen and a fluorescent microsphere-labeled gastrin 17 antibody; the second coating film comprises a first region, a second region, a third region, a fourth region and a fifth region which are separated; a formed fluorescent microsphere material comprises a polystyrene-methyl methacrylate copolymer. The kit and the method which are provided by the invention have the advantages of high sensitivity, high specificity, quickness, simplicity, capability of realizing objective measurement and the like.

The invention discloses a helicobacter pylori urease B antigen epitope polypeptide and application thereof. The amino acid sequence of the antigen epitope polypeptide is the sequence 34 in a sequence table, and the coding gene sequence of the antigen epitope polypeptide is the sequence 25 in the sequence table. The antigen epitope polypeptide provided by the invention can stimulate an organism togenerate helicobacter pylori resistance protective immunity reaction, thereby enhancing the effect of inhibiting helicobacter pylori infection, improving germ eliminating capacity of the organism, and playing an improving role in corresponding researches on development of vaccine and pathogenic mechanism and clinical diagnosis.

The invention provides preparation of zinc linolenate and application thereof in preparation of drugs for resisting helicobacter pylori. The preparation of the zinc linolenate comprises the steps of dissolving zinc chloride with sterile water, heating and activating a zinc chloride solution by using a reactor, and then naturally cooling the zinc chloride solution to the room temperature; dissolving 1-ethyl-3-(3-dimethyl aminopropyl) carbodiie hydrochlide, N-hydroxysulfosuccinimide sodium salt and linolenic acid in an EMS solvent, and activating in nitrogen; carrying heating reaction on activated zinc chloride and activated linolenic acid, and standing for cooling after reaction is finished; carrying out dialysis in pure water through a dialysis bag until the pure water is clear and transparent; and drying with a vacuum freeze dryer to obtain the zinc linolenate. The zinc linolenate is prepared successively, the yield is high, and the helicobacter pylori inhibiting effect of the zinc linolenate is excellent.

The invention relates to long-acting anti-helicobacter pylori toothpaste. The long-acting anti-helicobacter pylori toothpaste is prepared by the following components in parts by weight: 0.77-1.53% ofplant extract lysozyme, 0.77-2.30% of milk extract lactoferrin, 0.77-2.30% of a radix isatidis extract, 0.77-2.30% of a fructus forsythiae extract, 0.77-2.30% of a liquorice root extract, 0.77-2.30% of a hyacinth bletilla tuber extract, 0.77-2.30% of amino caproic acid, and the balance of a toothpaste matrix. The long-acting anti-helicobacter pylori toothpaste is capable of effectively killing andenduringly inhibiting pylori toothpaste, as well as eliminating halitosis and removing dental plaque; and moreover, the toothpaste is capable of reinforcing dentin and maintaining oral health so as to build a firewall to prevent pylori toothpaste from entering the stomach. In addition, the long-acting anti-helicobacter pylori toothpaste is simple in production process, few in production steps andlow in production cost.

The invention discloses a helicobacter pylori urease B antigen epitope polypeptide and application thereof. The amino acid sequence of the antigen epitope polypeptide is the sequence 31 in a sequence table, and the coding gene sequence of the antigen epitope polypeptide is the sequence 25 in the sequence table. The antigen epitope polypeptide provided by the invention can stimulate an organism togenerate helicobacter pylori resistance protective immunity reaction, thereby enhancing the effect of inhibiting helicobacter pylori infection, improving germ eliminating capacity of the organism, and playing an improving role in corresponding researches on development of vaccine and pathogenic mechanism and clinical diagnosis.

The invention provides biological toothpaste for resisting helicobacter pylori, and relates to the field of medical application. The biological toothpaste comprises the following substances in parts by weight: 25 parts of sorbitol, 1.5 parts of sodium carboxymethyl cellulose, 2.5 parts of sodium dodecyl sulfate, 13 parts of polyethylene glycol, 4 parts of calcium hydrogen phosphate, 20 parts of silicon dioxide, 0.3 part of xylitol, 1 part of broccoli essence, 0.5 part of eugenol, 0.5 part of tea tree essential oil, 0.3 part of lactoferrin, 1 part of probiotics and the balance of deionized water. In the biological toothpaste provided by the invention, the broccoli essence, lactoferrin and eugenol play a role in killing helicobacter pylori and cariogenic bacteria, the tea tree essential oilcan remove dental plaques and freshen breath, the xylitol can prevent dental caries, and the probiotics can balance oral floras. The raw materials are organically combined to prepare the toothpaste, which can play a role in eliminating oral helicobacter pylori, preventing dental caries and balancing oral floras, thereby realizing the purpose of the invention. The biological toothpaste provided bythe invention is simple to manufacture, is used as a daily necessity, is convenient and quick to use, economical and applicable, and good for long-term use, prevents and treats helicobacter pylori andcariogenic bacteria in an oral cavity, and keeps the oral cavity healthy.

The invention relates to a probiotic complex against helicobacter pylori and a preparation method of the probiotic complex. The probiotic complex is prepared from the following raw materials: 2 to 10parts by weight of lactobacillus helveticus, 10 to 25 parts by weight of bifidobacterium animalis, 5 to 15 parts by weight of bifidobacterium bifidum, 10 to 25 parts by weight of lactobacillus acidophilus, 2 to 15 parts by weight of lactobacillus rhamnosus, 1 to 6 parts by weight of bifidobacterium bifidum, 5 to 15 parts by weight of soy protein powder, 50 to 100 parts by weight of inulin, 20 to 50 parts by weight of resistant dextrin, 20 to 50 parts by weight of fructose-oligosaccharide, 2 to 5 parts by weight of flos lonicerae water extract and 10 to 20 parts by weight of prunus armeniaca fruit. The complex can achieve the purpose of eradicating the helicobacter pylori; in addition, the complex has the advantages of being free of side effects, fast in effect taking, thorough in treatmentin the use process and free of recurrence in a year after treatment.

The present invention relates to a chicken vitellus antibody (anti-HPTxIgY) with antibody activity for resisting pyloric helicobacterium cytotoxin, its preparation method and application in the preparation of medicine and health-care product for curing related diseases resulted from pyloric helicobacterium infection and preparation for detecting these related diseases. The above-mentioned antibody is undergone the process of IgY purity checking treatment. PAGE electrophoressi can be used for detecting purified vitallus antibody purity. Only one electrophoretic band is produced in the electrophoresis, and an UV spectrophotometer is used for determination so as to calculate the albumen concentration (mg / ml) which is (1.45XOD 280nm-0.74XOD 260nm)X dilute multiple. The above-mentioned method is as follows: preparing pyloric helicobacterium cytotoxin antigen, immunizing health hen with said antigen, collecting egg and extracting anti-HPTxIgY from the egg.

The immunological microsphere method of detecting pyloric helicobacterium in stool sample includes labeling microsphere to form immunological microsphere; suspending the tested stool sample containing pyloric helicobacterium in buffering liquid; mixing the immunological microsphere and the sample buffering liquid or mixing pyloric helicobacterium resisting antibody with the buffering liquid before adding pyloric helicobacterium resisting antigen labeled microsphere; and detecting pyloric helicobacterium in the stool sample. The method has the advantages of low cost, fast and convenient operation and reliable result.

The invention relates to Chinese medicinal herbal toothpaste comprising an anti-helicobacter pylori Chinese medicinal herbal active composition; the Chinese medicinal herbal toothpaste comprises a paste base and the anti-helicobacter pylori Chinese medicinal herbal active composition extracted by a supercritical CO2; wherein, the percentage of the Chinese medicinal herbal active composition accounted for the total weight of the Chinese medicinal herbal toothpaste is 0.5%-10%; and the remaining weight is the paste base. By adding the Chinese medicinal herbal active matter obtained by the technology of the supercritical CO2 extraction and separation into the toothpaste, the invention well solves the disadvantages of traditional extractive technique of the anti-helicobacter pylori Chinese medicinal herbal active composition, and the anti-helicobacter pylori Chinese medicinal herbal active composition with the definite composition is obtained; the purity of the anti-helicobacter pylori Chinese medicinal herbal active composition is very high, thereby being suitable for using as the medicated additive of the toothpaste. By adopting the invention, the Chinese medicinal herbal health care toothpaste which has high content of the anti-helicobacter pylori Chinese medicinal herbal active composition and good effect of inhibiting and killing the helicobacter pylori can be reliably obtained.

The invention relates to a carrier capable of showing and expressing helicobacter pylori protein on the surface of lactococcus lactis, and a construction method and application of the carrier. The construction method of the carrier comprises the following steps of: amplifying helicobacter pylori adhesin A (hpaA) gene by taking helicobacter pylori genomic deoxyribose nucleic acid (DNA) as a template by a polymerase chain reaction (PCR) method; performing enzyme digestion on the obtained hpaA gene and an expression carrier pNZ8110-lysM and connecting, wherein a connection product is used for transforming escherichia coli; screening and identifying positive transformation bacteria, and extracting recombinant plasmid pNZ8110-hpaA-lysM from the positive transformation bacteria; transferring the recombinant plasmid into a lactococcus lactis NZ3900 strain by an electroporation method, and screening and identifying to obtain a recombinant strain L.1actis NZ3900 / pNZ8110-hpaA-lysM; and culturing the recombinant strain, and introducing the expression of the hpaA gene by using an inductive agent Nisin. Western blots analysis shows that the protein of the cell wall of the recombinant strain contains the expressed HpaA protein which has immunocompetence. The expression carrier and the recombinant strain containing the carrier can be applied to preparation of helicobacter pylori resistance vaccine and a diagnostic reagent or food processing.

The present invention proposes a kit, the use of the kit in detecting pepsinogen I, pepsinogen II and anti-Helicobacter pylori antibodies, and the method of using the kit to detect pepsinogen I, pepsinogen II and anti-Helicobacter pylori antibodies method. The kit comprises: a first coating membrane; and a second coating membrane, one end of the first coating membrane is connected to one end of the second coating membrane; at least one area of ​​the first coating membrane is coated with pepsinogen labeled with fluorescent microspheres Ⅰ antibody pepsinogen Ⅰ antibody, fluorescent microsphere-labeled pepsinogen Ⅱ antibody and fluorescent microsphere-labeled first Helicobacter pylori antigen, the second coating membrane contains the separated first area, second area, third area and In the fourth area, the materials for forming fluorescent microspheres include: polystyrene-methyl methacrylate copolymer. The kit and method of the invention have the advantages of high sensitivity, strong specificity, rapidity, simplicity, objective determination and the like.

A Chinese medicinal collutory contains (wt%) anti-Helicobacter pylori Chinese medicinal active ingredient composition. The Chinese medicinal collutory comprises the anti-Helicobacter pylori Chinese medicinal active ingredient composition extracted by supercritical CO2 extraction And accounting for 0.5%-10% of the gross weight of the colluory, with the rest being collutory matrix. The collutory contains Houttuynia cordata active ingredients extracted by supercritical CO2 extraction and separation technology, so as to overcome the shortcomings in the conventional extraction technology of active ingredients from Chinese medicinal materials. The obtained anti-Helicobacter pylori Chinese medicinal active ingredient composition has white color and denifite composition; and is suitable for being used as medicinal additive of collutory. The collutory has good anti-Helicobacter pylori effect, high content of anti-Helicobacter pylori active ingredients, and improved quality.

A compound of the formula (I):wherein R1 represents an amino group which may be substituted; R2 represents a carboxy group which may be esterified or amidated; R3, R4, R5 and R6 each represent a hydroxy group which may be protected; Q represents an aryl group which may be substituted; or a salt thereof is disclosed. The compound (I) possesses ant-Helicobacter pylori activity, and is useful in the prevention or treatment of various diseases associated with Helicobacter bacteria, such as duodenal ulcer, gastric ulcer, chronic gastritis and cancer of the stomach.

The invention discloses a rapid detection method based on magnetic separation and quantum dot labeling of Helicobacter pylori and a kit, and the kit comprises an anti-Helicobacter pylori immunomagnetic bead nano magnetic bead and an anti-Helicobacter pylori quantum dot-labeled nanoprobe. The invention utilizes the advantages that the immuno-nanomagnetic beads can enrich the sample, the separationspeed is fast, the efficiency is high and the like, and combines the characteristics of high photochemical stability and high fluorescence intensity of the quantum dots, so that the detection system has the effect of multi-signal synergistic amplification to have ultra-high detection sensitivity. The invention is simple in detection method, fast in detection, easy to determine and low in detectioncost, can perform early diagnosis and prevention with high coincidence rate of the clinical diagnosis, has very high practical value in clinical diagnosis, pathogen identification, epidemiological investigation, etc., and is easy to promote.

The invention discloses a functional drink with resistance to helicobacter pylori. The functional drink comprises enzyme extract with volume ratio which is 10-80% and the rest of water, wherein 0.1-10g of active probiotics, 0.1-10g of liquorice and 0.05-5g of lactoferrin are added in each 100ml of the functional drink with the resistance to helicobacter pylori. The functional drink with the resistance to helicobacter pylori is simple in preparation and low in cost, has functions of aiding digestion, resisting against inflammation and helicobacter pylori, promoting cytothesis and improving immunity, and meanwhile, has good fruits and vegetables taste.

An application of chitosand and its delivative in preparing the medicine for treating the gastritis, gastric ulcer and duodenal ulcer, which are caused by pylorospirobacillus, is disclosed. Its action mechanism is also disclosed.