The invention discloses a photo-cured medical catheter hydrophilic lubrication coating and a preparation method thereof. The hydrophilic lubrication coating comprises a bottom coating and a lubrication coating, wherein the bottom coating is attached to the surface of an instrument, and the lubrication coating is attached to the bottom coating. The bottom coating comprises 1-10 parts of polyester acrylate, 50-90 parts of solvents, 0.5-5 parts of photoinitiators, 0.5-2 parts of wetting agents and 0.5-5 parts of reactive diluents; the lubrication coating comprises 1-10 parts of water soluble macromolecules, 1-5 parts of crosslinking macromolecules, 0-1 part of photoinitiators, 0.1-1 part of surfactants and 50-98 parts of mixed solvents. The preparation method of the hydrophilic lubrication coating is simple and easy in operation, substance residues caused by complicated high-temperature chemical reaction are avoided, the cured coating forms a crosslinking structure, has good adhesion on the surface of a medical catheter and has excellent and lasting lubricity in an aqueous medium, the friction coefficient of the surface of the medical catheter is reduced, and harm to human tissues andadhesion of macromolecules in blood are decreased.