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48results about How to "Infection Prevention and Control" patented technology

Chitosan emergent hemostasis material

The invention provides a chitosan emergent hemostasis material, which is provided with at least two layers of structures: a chitosan hemostasis layer as an upper layer, and a polyacrylic acid grafting chitosan lining layer as a lower layer, wherein the chitosan hemostasis layer is of a structure of a porous microsphere, porous fiber, porous sponge, or a compound of the porous microsphere, the porous fiber and the porous sponge; and the polyacrylic acid grafting chitosan lining layer is of a structure of porous fiber, porous sponge, or a compound of the porous fiber and the porous sponge. According to the chitosan emergent hemostasis material, the polyacrylic acid grafting chitosan is used as the lining layer of the chitosan, so that powerful water suction force can be provided, seepage velocity of blood in the chitosan hemostasis material is improved, blood is further concentrated, the density of the hemostasis material is improved simultaneously, the hemostasis material is easier to sink to arrive at a bleeding point, gravity press of a bleeding part is formed, pressurization is convenient, and the hemostasis effect is improved. Meanwhile, the polyacrylic acid grafting chitosan layer also can be used as a medicine-carrying substrate, can be used for carrying antibacterial drug, acesodyne, factors for promoting tissue repair and the like, and is good for preventing and controlling infection while stopping bleeding, relieving pains, and accelerating wound tissue healing.
Owner:欣乐加生物科技温州有限公司

Helicobacter pylori urease B antigenic epitope polypeptide and application thereof

The invention discloses a helicobacter pylori urease B antigenic epitope polypeptide and application thereof. The amino acid sequence of the antigenic epitope polypeptide is the sequence 37 in a sequence table, and the encoding gene sequence of the antigenic epitope polypeptide is the sequence 28 in the sequence table. The antigenic epitope polypeptide can stimulate organisms to generate helicobacter pylori resistant protective immune response so as to enhance the suppression of the helicobacter pylori infection, improve the capacity of removing germs of the organisms, and play a promoting role in development of corresponding vaccines, research on pathogenic mechanism, and clinical diagnosis.
Owner:INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE

Helicobacter pylori antigen epitope polypeptide and application thereof

The invention discloses a helicobacter pylori urease B antigen epitope polypeptide and application thereof. The amino acid sequence of the antigen epitope polypeptide is the sequence 34 in a sequence table, and the coding gene sequence of the antigen epitope polypeptide is the sequence 25 in the sequence table. The antigen epitope polypeptide provided by the invention can stimulate an organism togenerate helicobacter pylori resistance protective immunity reaction, thereby enhancing the effect of inhibiting helicobacter pylori infection, improving germ eliminating capacity of the organism, and playing an improving role in corresponding researches on development of vaccine and pathogenic mechanism and clinical diagnosis.
Owner:INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE

Helicobacter pylori urease B antigen epitope polypeptide and application thereof

The invention discloses a helicobacter pylori urease B antigen epitope polypeptide and application thereof. The amino acid sequence of the antigen epitope polypeptide is the sequence 31 in a sequence table, and the coding gene sequence of the antigen epitope polypeptide is the sequence 25 in the sequence table. The antigen epitope polypeptide provided by the invention can stimulate an organism togenerate helicobacter pylori resistance protective immunity reaction, thereby enhancing the effect of inhibiting helicobacter pylori infection, improving germ eliminating capacity of the organism, and playing an improving role in corresponding researches on development of vaccine and pathogenic mechanism and clinical diagnosis.
Owner:INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE

Specific nucleic acid aptamer and application

The invention provides sepecific nucleic acid aptamer. The nucleotide sequence of the specific nucleic acid aptamer is a sequence shown in the sequence table <400>1, the nucleic acid aptamer is screened through a whole bacterium reduction SELEX technique, is low in molecular weight and easy to synthesize and modify, can recognize enterohemorrhagic escherichia coli O157:H7 in a high-specific mode and be combined with the enterohemorrhagic escherichia coli O157:H7 in a high-affinity mode, does not specifically combined with other bacteria, can be used for detecting the enterohemorrhagic escherichia coli O157:H7 and has the important significant for effectively preventing and controlling infection of the enterohemorrhagic escherichia coli O157:H7.
Owner:INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL

Single clone antibody aiming at H3N2 dog flu virus HA2 protein

The invention provides a single clone antibody aiming at H3N2 dog flu virus HA2 protein, belonging to the technical field of biologics. The single clone antibody is prepared by inoculating an H3N2 dog flu virus Jiangsu separated strain JS / 10 separated in 2010 with SPF chicken embryos of 10 days old, collecting allantoic fluid of which the blood clotting titer is greater than or equal to 6, purifying virus inoculation MDCK cells, performing intracutaneous multipoint injection to inoculate mice, after cell fusion, performing three times of ELISA to detect cell supernate that cell colonies are generated, selecting an anti-H3 type hybridization tumor cell strain mAbD7, and recognizing and identifying the antigen so as to verify that the antibody aims at conservative H2A protein, wherein the neutralizing antibody titer is up to 1:12800, and the antibody subclass is IgG2b. The single clone antibody aiming at H3N2 dog flu virus and HA2 protein, which is provided by the invention, provides an important immunological preparation for treating H3N2 dog flu virus infection and has a potential application prospect.
Owner:NANJING AGRICULTURAL UNIVERSITY

External applied preparations for treating intractable skin ulcer

The invention relates to an externally applied preparation for treating skin wound, in particular to an externally applied preparation for treating chronic skin ulcer, which is characterized in that it comprises the effective components in weight as follows: insulin 500-5000u / 1000g, epidermal growth factor 0.001-0.01g / 1000g, antibacterials 5-20g / 1000g, others are adjuvant. It can treat a variety of infectious and protracted skin ulcer safely and effectively.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Preparation method for goose-origin reovirus inactivated vaccine

The invention provides a preparation method for a goose-origin reovirus inactivated vaccine. The preparation method comprises the following steps that a goose embryo is inoculated against reoviruses through an allantoic cavity, dead embryo allantoic fluids or live embryo allantoic fluids are collected within 24-144 h after incubation, and reovirus fluids are obtained; the reovirus fluids are inactivated through formaldehyde, Tween-80 is added to be mixed to serve as a water phase, and white oil, Span-80 and aluminium stearate are mixed to serve as an oil phase; and the water phase is added into the oil phase to be mixed uniformly, and the inactivated vaccine is obtained. According to the preparation method, the prepared goose-origin reovirus inactivated vaccine has high protective effects on a gosling, and is suitable for virus immunity of the gosling.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Duck parvovirus strain and live vaccine thereof

The invention relates to the field of animal virology and provides a duck parvovirus strain, live vaccine thereof and a preparing method. The strain is a duck parvovirus and specifically named GT2015v, and the biology preservation serial number of the strain is CCTCC No:V201528; the vaccine prepared by utilizing the strain effectively solves the problem that the disease can not be prevented at present in China, by means of continuous passage of duck embryos, a medium virulence isolate strain is attenuated and made to lose pathogenicity to ducks, good immunogenicity is kept, duck parvovirus infection can be prevented and controlled safely and effectively, cultivation risks are reduced, and sound development of duck breeding industry is guaranteed.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Vaccine for preventing toxoplasma infection and application thereof

The invention provides a vaccine for preventing toxoplasma infection. The vaccine contains nucleotide sequences selected from the followings: 1) 736-2385th nucleotide sequences as shown in SEQ ID No.1, and / or 759-2411th nucleotide sequences as shown in SEQ ID No.3; 2) nucleotide sequences complementary with the nucleotide sequences as shown in 1); and 3) nucleotide sequences which perform substituting, deletion and addition modification on one or a plurality of basic groups of the nucleotide sequences as shown in 1) or 2). The vaccine has the capability of effectively preventing and controlling the infection of a toxoplasma animal model (Kunming mice), and the combination of recombinant plasmids can effectively improve the immune effect of a single-gene vaccine, therefore, plasmids pVAX-ROP5 and pVAX-GRA15 can be used as DNA (Deoxyribose Nucleic Acid) vaccine candidate antigens for preventing the toxoplasma infection; and a combined immune vaccine of a recombinant plasmid based on such two genes can be used as a cocktail composite vaccine for preventing the toxoplasma infection.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

Recombinant OmpK multi-epitope polypeptide, construction method and application thereof

The invention relates to the field of biomedicines and in particular relates to a recombinant polypeptide, a construction method and application thereof. More specifically, the invention provides a recombinant OmpK multi-epitope polypeptide and encoded nucleotide, carriers, cells and vaccines thereof, and application of the recombinant OmpK multi-epitope polypeptide to products of preventing vibrio infections. An experiment proves that the recombinant OmpK multi-epitope polypeptide or derivatives and vaccines thereof have the characteristics of safety and high efficiency and have very high immunogenicity on vibrio parahaemolyticus; animals immunized by the multi-epitope polypeptide contain a lot of specific antibodies and the effective protection rate on the immunized animals reach 90 percent or more; the recombinant OmpK multi-epitope polypeptide has the effect of improving the resistance capability of the animals immunized by the multi-epitope polypeptide on the vibrio parahaemolyticus; the recombinant OmpK multi-epitope polypeptide has a good immune protection effect and has great significance in the aspect of preventing and controlling infectious diseases of aquatic animals.
Owner:中华人民共和国汕头海关

Vaccine used for infection prevention of toxoplasma gondii, preparation method and application of vaccine

The invention firstly provides a nucleotide sequence used for infection prevention of toxoplasma gondii. The nucleotide sequence is selected from nucleotide sequences as follows: 1), a nucleotide sequence represented in a sequence table SEQIDNo.1; 2), a nucleotide sequence which is complementary with the nucleotide sequence represented in 1); and 3), a nucleotide sequence which performs substitution, loss and adding modification on one or more basic groups of the nucleotide sequence represented in 1) or 2) and has a toxoplasma gondii infection prevention function. The invention further provides a vaccine which comprises the nucleotide sequence and a medically acceptable carrier. The plasmid DNA vaccine pVAX-HSP60 can effectively prevent and control infection of a toxoplasma gondii animal model (a Kunming strain mouse), that is, the survival time of the mouse can be effectively prolonged, and worm count of brain cyst is reduced, so that the plasmid pVAX-HSP60 can be used as a DNA vaccine candidate antigen for resisting infection of toxoplasma gondii.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

Endoglin nucleic acid aptamer based on CRISPR/Cas9 and application

InactiveCN109576274AAvoids differences in native conformationAccurate targetBiological testingDNA/RNA fragmentationAptamerEndoglin
The invention discloses Endoglin nucleic acid aptamer based on CRISPR / Cas9 and application, and belongs to the technical field of genetic engineering, immunology and oncology. The Endoglin nucleic acid aptamer is any one or more sequences in nucleotide sequences shown in SEQ ID NO: 1-4. The endoglin nucleic acid aptamer is screened by a whole-cell subtraction Cell-SELEX technology, the molecular weight is small, the endoglin nucleic acid aptamer is easy to synthetize and modify, can recognize Endoglin in a high-specificity mode and can be bonded with Endoglin in a high-affinity mode, does notconduct feature bonding with other cells, is free of immunogenicity, is stable and easy to modify, and is convenient to synthetize and store. An Endoglin detection kit and reagent paper prepared by the Endoglin nucleic acid aptamer have high detection sensitivity to Endoglin, and are prone to optimization. The Endoglin nucleic acid aptamer is applied to assessment of tumor diagnosis and tumor prognosis and monitoring, and tumor diagnosis.
Owner:GUANGXI MEDICAL UNIVERSITY

Primer, probe and kit for detecting and typing rickettsia by virtue of real-time FRET-PCR (Fluorescent Resonance Energy Transfer-Polymerase Chain Reaction) and nested PCR

The invention relates to a primer, a probe and a kit for detecting and typing a rickettsia by virtue of real-time FRET-PCR (Fluorescent Resonance Energy Transfer-Polymerase Chain Reaction) and nested PCR. The primer and the probe are shown as sequences 1-8. By virtue of designing the primer and the probe of the FRET-PCR, nucleinic acids of all Rickettsiaes can be specifically amplified, thus fast judging positive samples; then two pairs of primers of the nested PCR can be designed by selecting other conservative intervals of the same gene, and the Rickettsiae of the corresponding positive samples can be determined by virtue of nested PCR, agarose gel electrophoresis and sequencing. Therefore, the system not only can fast and specifically detect the rickettsia organisms of all the Rickettsiaes, but also can confirm the Rickettsiae.
Owner:YANGZHOU UNIV

Chitosan emergent hemostasis material

The invention provides a chitosan emergent hemostasis material, which is provided with at least two layers of structures: a chitosan hemostasis layer as an upper layer, and a polyacrylic acid grafting chitosan lining layer as a lower layer, wherein the chitosan hemostasis layer is of a structure of a porous microsphere, porous fiber, porous sponge, or a compound of the porous microsphere, the porous fiber and the porous sponge; and the polyacrylic acid grafting chitosan lining layer is of a structure of porous fiber, porous sponge, or a compound of the porous fiber and the porous sponge. According to the chitosan emergent hemostasis material, the polyacrylic acid grafting chitosan is used as the lining layer of the chitosan, so that powerful water suction force can be provided, seepage velocity of blood in the chitosan hemostasis material is improved, blood is further concentrated, the density of the hemostasis material is improved simultaneously, the hemostasis material is easier to sink to arrive at a bleeding point, gravity press of a bleeding part is formed, pressurization is convenient, and the hemostasis effect is improved. Meanwhile, the polyacrylic acid grafting chitosan layer also can be used as a medicine-carrying substrate, can be used for carrying antibacterial drug, acesodyne, factors for promoting tissue repair and the like, and is good for preventing and controlling infection while stopping bleeding, relieving pains, and accelerating wound tissue healing.
Owner:欣乐加生物科技温州有限公司

External application wound repair liquid

The present invention provides an external application wound repair liquid, which is characterized in that the repair liquid is prepared from sanguisorba, common bletilla tuber, japanese honeysuckle stem, and 75% ethanol. A preparation method of the external application wound repair liquid comprises: crushing the three herbs, uniformly mixing and filling into a container, adding the 75% ethanol, standing for a certain time, and carrying out repeated filtration to remove residues to obtain the external application wound repair liquid of the present invention. The external application wound repair liquid of the present invention can be used for various types of wounds, and has advantages of significant effect, simple preparation, easy use, and the like.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Nucleic acid enzyme surface protein of streptococcus suis 2-type and preparation method and application thereof

The invention discloses a nucleic acid enzyme surface protein of streptococcus suis 2-type and also discloses a preparation method and application of the protein. The number of the nucleic acid enzyme surface protein of the streptococcus suis 2-type in a genebank is gi (146321396); the preparation method comprises: cloning a nucleic acid enzyme surface protein gene; connecting the gene to colibacillus expression plasmid, and transforming colibacillus; inducing expression; and separating and purifying an expression product and other steps. The nucleic acid enzyme surface protein can be used asa diagnostic target; and a specific antibody of the protein can be detected in animals and people infected with the streptococcus suis. Simultaneously, the protein is also an important protective antigen and can be used for preparing vaccine.
Owner:MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI

Klebsiella oxytoca for expressing luciferase and application of klebsiella oxytoca

The invention constructs Klebsiella oxytoca containing luciferase gene cluster (luciferase gene cassette, lux) by utilizing plasmid construction and an electric transformation technology. Through autofluorescence identification, a subculture stability test of strains and observation of culture characteristics, the Klebsiella oxytoca (KOX-LUX) capable of stably expressing luciferase is finally obtained. An important visual research tool is provided for researching the progress of Klebsiella oxytoca related diseases and screening proper sterilization methods, antibiotics and the like.
Owner:BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV

PCV2, PCV3 and PCV4 triple subunit vaccine as well as preparation method and application thereof

The invention relates to a PCV2, PCV3 and PCV4 triple subunit vaccine and a preparation method thereof, the PCV2, PCV3 and PCV4 triple subunit vaccine comprises three recombinant proteins, namely a PCV2 Cap protein, a PCV3 Cap protein and a PCV4 Cap protein, the PCV2 Cap protein is obtained by expressing a gene sequence as shown in SEQ ID NO.1 through escherichia coli, the PCV3 Cap protein is obtained by expressing a gene sequence as shown in SEQ ID NO.2 through escherichia coli, and the PCV4 Cap protein is obtained by expressing a gene sequence as shown in SEQ ID NO.2 through escherichia coli. The PCV4Cap protein is obtained by expressing a gene sequence as shown in SEQ ID NO.3 through escherichia coli. The invention also discloses a preparation method of the PCV4Cap protein. The vaccine can be used for preventing and treating PCV2, PCV3 and PCV4 infection at the same time, the immunogenicity aiming at PCV2, PCV3 and PCV4 is remarkably improved, and immunized animals can be induced to generate high-titer antibodies.
Owner:JILIN UNIV

High-wine-yield Klebsiella expressing luciferase and application thereof

A plasmid construction technology and an electro-transformation technology are used to establish high-wine-yield Klebsiella containing a luciferase gene cluster (lux). Through auto-fluorescence identification, a subculture stability test of a strain, and observation on culture characteristics, high-wine-yield Klebsiella (HiAlc Kpn-LUX) capable of stably expressing luciferase is finally obtained. An important visual research tool is provided for researching the progress of wine-producing Klebsiella related diseases and screening proper sterilization methods and antibiotics.
Owner:BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV

Muddy cranberry beverage and preparation method of muddy cranberry beverage

The invention discloses a muddy cranberry beverage. The muddy cranberry beverage is prepared from the following components in parts by weight: 21 to 26 parts of cranberry, 12 to 14 parts of semen nelumbinis, 8 to 10 parts of ginkgo fruit, 9 to 13 parts of fructus lycii, 8 to 10 parts of rosemary, 14 to 16 parts of rhoeo discolor, 4 to 6 parts of white granulated sugar, 0.01 to 0.03 part of a preservative and 0.2 to 0.4 part of an acidity regulator. The muddy cranberry beverage prepared by the invention effectively keeps nutrient substances in the raw materials and the prepared beverage has abundant nutrients and can be used for effectively preventing and treating various daily female urinary system symptoms including bacterial infection, urethritis, cystitis and chronic pyelonephritis; after being orally taken for a long period, the muddy cranberry beverage can be used for effectively alleviating symptoms including female dysmenorrhea and the like.
Owner:BENGBU KUNPENG FOOD & BEVERAGE

Sacral nerve electrical stimulation system

InactiveCN110327548AChange in urination patternImprove urinationElectrotherapyArtificial respirationVoltage pulseSacral nerve stimulation
The invention discloses a sacral nerve electrical stimulation system, relates to the field of medical apparatus and instruments, and solves the problem that an existing sacral nerve electrical stimulator is liable to harm patients. A control unit comprises a boosting amplitude modulation unit, a pulse signal amplification unit, a master control unit, a human-computer interaction unit and a communication unit, wherein the master control unit is independently connected with the boosting amplitude modulation unit, the pulse signal amplification unit, the human-computer interaction unit and the communication unit; an emitter is connected with a receiver through the communication unit; the mater control unit controls the boosting amplitude modulation unit to output an adjustable voltage pulse signal; the pulse amplitude value of the adjustable voltage pulse signal is amplified to a required numerical value through the pulse signal amplification unit, and then, the required numerical value is transmitted to the emitter; the pulse signal emitted through the emitter is received through the receiver in a body to realize pulse signal stimulus for a corresponding neure; and the human-computerinteraction unit is used for displaying each parameter value of the pulse signal. The system is favorable for improving the bladder urination function of a spinal cord injury patient, and preventingand controlling urinary system infection.
Owner:JILIN UNIV

Surface protein of streptococcus suis type-2, preparation method thereof and application

The invention discloses a surface protein of streptococcus suis type-2, preparation method thereof and application. The surface protein of streptococcus suis type-2 is numbered as gi|146321522 in genebank; the preparation method of the surface protein comprises the following steps: cloning gene of the surface protein; connecting the gene and expression plasmid of Escherichia coli to transform the Escherichia coli; inducing expressing; separating and purifying expression product; and the like. The surface lipoprotein can be used as a diagnostic target, and a specific antibody of the protein can be detected in animals and people infected with the streptococcus suis; moreover, the surface lipoprotein is also an important protective antigen, and can be used for preparing vaccines.
Owner:MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI

Multifunctional ozone water health care machine

The invention discloses a multifunctional ozone (blue oxygen) water health care machine. The machine comprises an ozone water generating device of which the water outlet end is connected with a heater, and the heater is connected with a flushing device and a water outlet handle used for cleaning and nursing of anorectum and / or skin through pipelines; a flushing head of the flushing device is provided with a second water outlet used for cleaning and nursing of buttocks and a third water outlet used for cleaning and nursing of private parts; the flushing device is also provided with a first water outlet used for cleaning the flushing head. The water outlet end of the ozone water generating device is connected to the flushing device through a pipeline and communicated with the first water outlet of the flushing device. The machine adopts several gas-liquid mixing technologies (blue oxygen tow mixing, jet mixing, air bubble mixing or blue oxygen electrolytic direct mixing), effectively dissolves ozone gas in water to make high-concentration ozone water, is used for washing and cleaning of anus, removal of rectum overnight stools, relieving and eliminating of hemorrhoid symptoms, cleaning and nursing of the buttocks and women's private parts and the like, and has dual functions of treatment and protection.
Owner:NINGBO HUISHIKANG HEALTH SCI & TECH

Helicobacter pylori urease B antigen epitope polypeptide and application thereof

The invention discloses a helicobacter pylori urease B antigen epitope polypeptide and application thereof. The amino acid sequence of the antigen epitope polypeptide is the sequence 31 in a sequence table, and the coding gene sequence of the antigen epitope polypeptide is the sequence 25 in the sequence table. The antigen epitope polypeptide provided by the invention can stimulate an organism togenerate helicobacter pylori resistance protective immunity reaction, thereby enhancing the effect of inhibiting helicobacter pylori infection, improving germ eliminating capacity of the organism, and playing an improving role in corresponding researches on development of vaccine and pathogenic mechanism and clinical diagnosis.
Owner:INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE

A middle-aged and elderly goat milk powder nutrition enhancer and preparation method thereof

The invention belongs to the technical field of food production, and particularly relates to a nutrient enhancer for middle-aged goat milk powder and a preparation method thereof. The formula of the nutrient enhancer for middle-aged goat milk powder includes the following raw materials in parts by weight: 10-15 parts of an oat powder, 1-2 parts of a cranberry fruit powder, 1-3 parts of a Se-enriched germ brown rice powder, 1-2 parts of a purple yam powder, 1-2 parts of an almond powder, 1-2 parts of a flaxseed powder, 1-2 parts of a red date powder, 2-3 parts of a pueraria powder, 1-2 parts of lupeose, 2-3 parts of an apple juice powder and 1-3 parts of isomaltose hypgather. The nutrient enhancer adopts a scientific and reasonable formula integrating grains, fruit and yam, and the plant nutrient is comprehensive and sufficiently complementary to the holozoic nutrition in goat milk; the enhancer can enhance the nutrient in the goat milk and promote the absorbtion of the nutritional ingredients in the goat milk; in addition, the enhancer can condition the intestinal tract, help human bodies to discharge toxics, scavenge free radical, defer senility, and improve human immunity. The tests, such as an LD50 acute toxicity test, a 30-day feeding test, an Ames test, a mice bone marrow polychromatic erythrocytes micronucleus test, a mice sperm shape abnormality test, and a crowd efficacy observation test, are carried out, and the test results show that the nutrient enhancer is non-toxic and effective.
Owner:南京贝杉国际贸易有限公司
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