1201 results about "Immunocompetence" patented technology

The invention includes compositions and methods for enhancing immunopotency of an immune cell by way of inhibiting a negative immune regulator in the cell. The present invention provides vaccines and therapies in which antigen presentation is enhanced through inhibition of negative immune regulators. The present invention also provides a mechanism to break self tolerance in tumor vaccination methods that rely on presentation of self tumor antigens.

The present invention provides a clinically applicable method of stem cell transplantation that facilitates engraftment and reconstitutes immunocompetence of the recipient without requiring radiotherapy or chemotherapy, and without development of GVHD or graft rejection. Aspects of the present invention are based on the discovery that the depletion of the endogenous stem cell niche facilitates efficient engraftment of stem cells into that niche. In particular, the present invention combines the use of selective ablation of endogenous stem cells, in combination with the administration to the recipient of exogenous stem cells, resulting in efficient, long-term engraftment and tolerance.

The invention relates to a traditional Chinese medice healthy liquid. The preparation method comprises the following steps of: preparing 73 materials of ginseng, atractylodes macrocephaia, poria, and the like into a stock solution; then adding 8 materials of ginseng, cortex encomia, astragalus root, and the like to water for decoction, wherein the 8 materials and the water are in a weight ratio of 1:25; decocting until the weight ratio of the raw materials to the water is 1:20 to obtain an auxiliary solution; proportioning the stock solution and the auxiliary solution in a weight part ratio of 1:4 and adding a proper amount of auxiliary materials; and mixing and standing for 6 months to obtain the healthy liquid. The healthy liquid can improve the immunological competence of human bodies after being taken, release fatigue, smooth the kidneys and activate the blood circulation. Moreover, the healthy liquid has simple preparation process and is easy for industrialization production.

The invention discloses a preparation method of a biological feed additive with nutrition and immunocompetence. The biological feed additive with nutrition and immunocompetence comprises the following components: 0.05 to 0.1% of aerobic bacteria, 0.05 to 0.1% of anaerobic bacteria, 0.3 to 0.5% of fermenting promoter, 5 to 15% of soybean meal, 5 to 10% of molasses, 15 to 25% of DDGS (Distillers Dried Grains with Soluble), 10 to 30% of cassava residue, 20 to 50% of bran meal, 5 to 15% of bran, 5 to 15% of maize cob powder, and 20 to 40% of maize slurry protein. The preparation method comprises the processes of activating bacteria, crushing raw materials, aerobically fermenting for 1 to 2 days, and then anaerobically fermenting for 3 to 7 days. The prepared biological feed additive brings piglet, medium and large pigs and sheep with high nutrition value, is high in palatability, and has effects of accelerating growth, improving intestinal health, controlling diarrhea, controlling constipation of sow, increasing birth weight and weaning weight of the piglet, improving the quality of eggshell and egg yolk, controlling feed-containing faeces, raising the lactation level of dairy cattle, as well as increasing butterfat and protein content.

The invention discloses a health-care sausage capable of expelling toxin and moisturizing skin,which comprises the following needed raw materials in parts by weight: 15-30 parts of rhizome dioscoreae, 0.5-2 parts of astragalus mongholicus, 1-2 parts of poria cocos, 0.5-1.5 parts of angelica sinensis, 8-15 parts of kelp, 3-4 parts of hawthorn, 20-35 parts of pig blood, 1-3 parts of boxthorn fruit, 10-20 parts of black fungus, 5-10 parts of champgnon, 35-58 parts of bean curd, 13-22 parts of chicken, 5-10 parts of starch and 3-8 parts of seasoner. The health-care sausage of the invention has delicious taste, balance nutrition, reasonable formula and convenience in eating, and has the health-care effects of nourishing vitality and blood, replenishing the vital essence and improving the eyesight, eliminating the rubbish in the body, regulating the pH value of blood, lowering the blood pressure, the blood sugar level and the blood fat, tonifying the spleen and the stomach, and moistening the intestines and freeing the stool, and can moisten the skin, improve the looks, delay the aging and enhance the immunocompetence of human bodies after being eaten frequently. In the health-care sausage of the invention, the sausage species are increased, thus leading the traditional sausage to have nutrition.

The present invention provides an efficient gene delivery system using Adeno-Associated Viral (AAV) vector in gene therapy. Furthermore, the invention provides a combined AAV and Adenovirus (Adv) cocktail gene delivery system which is even more efficient in in vivo gene delivery and expression without eliciting any significant immune responses in an immunocompetent subject. In particular, the invention provides a therapeutic agent and methods for preventing, treating, managing, or ameliorating various diseases and disorders including, but not limited to, bone diseases, by delivering Bone Morphogenetic Protein 2 (BMP-2) for new bone formation via gene therapy using said system. The invention provides a nucleic acid molecule comprising an AVV vector and a promoter operably linked to a sequence encoding BMP-2; and a nucleic acid molecule comprising an Adv vector and a promoter operably linked to a sequence encoding BMP-2, as well as vectors and host cells comprising said nucleic acid molecules, respectively.

The present invention provides a soil conditioner containing marine organisim, composed of 10-40 (weight)% shell powder, 10-60 (weight)% oyster shell powder, 1-5 (weight) % chitosan powder, 10-50 (weight) % stem vegetable and fruit powder or leavening having strong fibre, and or marine organisim powder or leavening. The waste marine organisim is a main raw material, the raw material is easy to get, having low cost, and the waste is effectively utilized, beneficial to environmental protection. The soil conditioner improves soil hardening, increasing soil permeability and activity, eliminating residual in the soil of the decomposition chemical fertilizer. The soil conditioner enhances the fertilizer utilization ratio, also exciting cropper root growth and enhancing cropper immunocompetence.

The invention provides a Chinese sausage containing dietary fibers and fish meat and a production method thereof, which can make up for the shortage of the current sausage market. The technical scheme is as follows: the weight percentage of the dietary fibers is 2 percent to 20 percent, the weight percentage of the fish meat is 10 percent to 40 percent, the weight percentage of livestock meat is 30 percent to 70 percent, the weight percentage of seasoning is 5 percent to 15 percent, and the rest is water. The Chinese sausage has functions, such as preventing constipation and intestinal diseases, eliminating heavy metals and radicals in the body, preventing cardiovascular diseases, diabetes and breast cancer, preventing and treating gallstone, reducing blood pressure and enhancing the immunocompetence of the human body.

The invention discloses an enteromorpha polysaccharide having immunocompetence and a preparation method thereof. The enteromorpha polysaccharide having immunocompetence is a polysaccharide HT-II. The enteromorpha polysaccharide having immunocompetence is a white floccule, can be well dissolved in water and dimethyl sulfoxide, has relative molecular weight of 96kDa, and mainly comprises glucose and mannose, wherein a ratio of rhamnoside to xylose to mannoside to glucose is 36: 20: 6: 38. The preparation method comprises the following steps of breaking down, carrying out extraction at a temperature of 90 DEG C by water, filtrating, merging the extract, concentrating, carrying out precipitation by ethanol, removing proteins, carrying out separation and purification, and carrying out freeze drying to obtain the purified enteromorpha polysaccharide having immunocompetence. The preparation method is simple, has stable performances, a low cost and a large medical value, can reduce cholesterol content and can improve immunity. The preparation method adopts extraction, protein removal and purification processes, and scientific experimental means, is a novel method for controlling enteromorpha, and provides a novel approach for controlling enteromorpha. The preparation method of the enteromorpha polysaccharide having immunocompetence develops enteromorpha medical values and purposes, controls environmental pollution, reduces a clean-up cost, improves economic benefits and is an ideal special preparation technology for enteromorpha control and integrated utilization.

The invention relates to a medical testing kit for performing quantitative detection on human serum RBP4 using chemiluminescence magnetic-enzyme immunotherapy. The kit is composed of four reagent parts: specificity mouse anti-human RBP4 custodite immunomagnetic beads, enzyme labelling specificity mouse anti-human RBP4 antibody II, chemiluminescence substrate, corresponding titer and quality control liquid. The using method of the kit comprises: using bead particulates as solid phase carrier, combining specificity mouse anti-human RBP4 antibody I on the surface, forming RBP4 specificity immunocompetence beads, capturing antigen RBP4 to be detected in the enzyme labelling specificity mouse anti-human RBP4 antibody II, forming double antibody sandwich composite on the surface of the beads, wherein enzyme marked on the composite reacts with corresponding irradiance substrate in the reaction system to form stable luminous signals, thereby reaching quantitative detection and analysis on RBP4 through strength of the detection light signals. The invention has the advantages of high sensitivity, high specificity, simple and fast operation.

The invention relates to a healthy crawfish feed and a preparation method. The healthy crawfish feed is prepared by selecting various organic raw materials according to a formula, thus providing feed base for producing high-quality crawfish; especially, natural fish meal, earthworm meal, silkworm chrysalis meal, spiral shell meal and bone tankage are added to provide a high-quality animal protein source for the high-quality crawfish, thus enabling the nutrition ingredients of the feed to be more reasonable; due to addition of Chinese herbal medicines, the immunocompetence of the high-quality crawfish is favorably enhanced, the fish flavor of the crawfish is improved, the disease infection possibility can be reduced, and the shell powder can provide calcium for manufacturing crawfish shells; the feed prepared by adopting the formula is complete in nutrition, good in palatability, easy to digest, and fast to absorb, and the crawfish is hale and healthy to grow, strong in disease prevention capability, and high in culture survival rate; and the preparation method is wide in raw material sources, simple and convenient as well as easy to operate, reasonable in formula, and low in cost.

The present invention discloses a recombinant baculovirus strain rBac / PCV2Cap (microorganism preservation number: CGMCC NO.2083) efficiently expressing Porcine circovirus type 2 Cap protein and applications thereof. The recombinant baculovirus strain rBac / PCV2Cap constructed by the present invention can efficiently express recombinant PCV2-Cap protein in insect cells, and the expressed recombinant Cap protein, which has good immunocompetence and antigenicity, can serve as a subunit vaccine used to prevent the related plague caused by Porcine circovirus type 2 infection as well as a detecting and diagnostic antigen for Porcine circovirus type 2 serum antibody.

The invention discloses a combination used for preventing and treating newborn piglet diarrhea and a preparation method and application thereof. In percent by weight, the product comprises 88-98% of attapulgite ultra-fine powder, 0.1-3% of organic acid, 0.1-4% of probiotic, 0.05-2% of oligosaccharide, 0.05-1.5% of vitamin C and 0.15-3% of crane sugar. The combination disclosed by the invention isdiluted into solution with the mass fraction of 0.05-3% with drinking water, the solution is used for feeding a piglet in an oral or drinking water manner, and the newborn piglet diarrhea caused by multiple factors can be effectively prevented and treated, thus the combination has the effects of improving immunocompetence of the piglet, improving antioxidant function, regulating intestinal microecology and protecting intestinal shape and can improve survival rate of the piglet, improve production performance of the piglet and relieve weaned stress on the piglet. The combination disclosed by the invention also has the characteristics of simple and convenient application mode, high efficiency, available raw materials, simple preparation and the like.

The present invention relates to a bovine VDJ cassette (BF1H1) that provides the novel ability to develop chimeric immunoglobulin molecule capable of incorporating both linear T cell epitope(s) (CDR1H and CDR2H) as well as conformational B cell epitope(s) (exceptionally long CDR3H). Further, multiple epitopes can be incorporated for development of multivalent vaccine by replacing at least a portion of an immunoglobulin molecule with the desired epitope such that functional ability of both epitope(s) and parent VDJ rearrangement is retained. The antigenized immunoglobulin incorporating both T and B epitopes of interest is especially useful for development of oral vaccines for use in humans apart from other species including cattle. The long CDR3H in BF1H1 VDJ rearrangement originates from long germline D-genes. The novel bovine germline D-genes provide unique molecular genetic marker for sustaining the D-gene pool in cattle essential for immunocompetence via selective breeding. D-gene specific DNA probe permits typing and selection of breeding cattle stock for maximum gemline D gene pool for better health and disease prevention. The bovine D-genes are unique to cattle and, therefore, provide sensitive and specific forensic analytical tool using molecular biology techniques to determine tissues suspected of bovine origin.

The invention provides feed for improving the digestive and immunologic ability of chicken, which comprises a feed carrier, choline chloride, lysine hydrochloride, phenylalanine dihydrochloride, pantothenic acid, clostridium butyricum, lactobacillus plantarum, sodium butyrate, stone powder, folic acid, riboflavin, vitamin E, vitamin K, glucolase, amylase, sodium selenate, magnesium sulfate and a Chinese herbal medicine composition additive. The feed has the beneficial effect that a digestion system and an immune system of the chicken can be improved, so that the feeding management and epidemic prevention works during the brooding period are better improved.

The invention relates to a polysaccharide buccal tablet prepared from Camellia nitidissima, which mainly comprises the following raw materials: polysaccharide substances extracted from the Camellia nitidissima, a sweetening agent, trehalose, citric acid and the like. The preparation method comprises the following steps: adopting supersonic waves to leach the Camellia nitidissima first, adopting a nano-filtration method to perform concentration production of a concentrated Camellia nitidissima solution, performing spray drying, tabletting and package, and obtaining the product. The product has the functions of reducing blood sugar, blood pressure, blood fat and cholesterol in serum, inhibiting tumor growth, preventing atherosclerosis, activating various enzymes in human body, improving the immunological competence of the body, prolonging senescence, improving digestion of intestinal canals, and the like, has complete health-care functions, is suitable to be eaten by common people, and has obvious health-care effect.

The invention discloses a formula feed of broilers and a preparation method thereof. The formula is as follows: 50-65% of corn, 0-2% of fishmeal, 20-25% of soya bean meal, 0-3% of corn protein powder, 0-1% of whole hen egg, 0-5% of wheat middling, 0-5% of rice pericarps, 0-5% of rice bran, 0-3% of cottonseed meal, 0.5-1.5% of bean oil, 0.1-0.3% of acidulant, 0.05-0.1% of sodium butyrate, 0.03-0.05% of compound enzyme preparation and 1.5-2% of compound premix. The feed is prepared by the steps of removing impurities from raw materials, grinding raw materials, weighing raw materials, mixing raw materials, palletizing and packing finished products, etc. in sequence. The invention selects the additives which promote the broilers to grow and develop and the feed materials which improve the immunocompetence, etc. The feed product has moderate metabolic energy value, high nutrition level and good palatability, is easy to digest and absorb and is easy to satisfy the nutritional requirements of 1-7 days of growth, therefore, the broilers grow fast and have high disease resistance, low mortality and low feed coefficient, and the coefficient of the formula feed is 0.8-0.9.

The invention discloses a traditional Chinese medicine composition for treating gastric cancer, and relates to the technical field of traditional Chinese medicines, to solve the problem that no effective medicine is available for treating the gastric cancer. The traditional Chinese medicine composition is the traditional Chinese medicine preparation comprising burdock, fig, gallnut, gallnut, daemonorops draco, manis pentadactyla, astragalus mongholicus, angelica sinensis and liquorice. The traditional Chinese medicine composition disclosed by the invention has high anti-cancer activity for solid tumor such as gastric cancer, and has few side effects, is capable of improving the immunocompetence of a patient, and is suitable for the clinical patient with the gastric cancer.

The invention discloses a preparation method and application of a carbon nanomaterial-immunostimulatory sequence compound. The preparation method comprises the steps of (1) modifying a carbon nanomaterial through polylysine to obtain a polylysine-modified carbon nanomaterial; and (2) mixing the polylysine-modified carbon nanomaterial obtained from the step (1) with an immunostimulatory sequence in a water solution, oscillating for 0.5-3 hours at 20-37 DEG C, and centrifuging, collecting and depositing to obtain the carbon nanomaterial-immunostimulatory sequence compound. According to the carbon nanomaterial-immunostimulatory sequence compound, the carbon nanomaterial serves as a carrier for intracellular transport of the immunostimulatory sequence (CpG DNA), so as to remarkably improve the cellular uptake efficiency of the CpG DNA, protect the CpG DNA from being degraded by nuclease and enhance the immunocompetence of an organism for a long time, so the carbon nanomaterial-immunostimulatory sequence compound has a good medical application prospect.

The present invention relates to methods and immunonutritional compositions for preventing the impairment of the immune function during anti-cancer therapy, thereby attaining a better efficacy of the treatment. More particularly, the present invention relates to methods and immunonutritional compositions that can transiently preventing or moderating, bone marrow paralysis or neutropenia of a subject undergoing anti-cancer therapy-induced apoptosis or necrosis or other cell damage such that the innate and adaptive immune functions and normal physiology of the bone marrow are preserved, at least in part, which, in turn, lead to (i) a better tolerance and increased efficacy to anti-cancer therapy; (ii) transient augmentation or enhancement of immunocompetence of the immune cell; and (iii) optimization of the effects of and increase of immunocompetence of the immune cell weakened by anti-cancer therapy.

The invention relates to sterile bovine colostrum whey with natural immunocompetence, and a preparation method and an application thereof. The preparation method comprises the steps of centrifuging bovine colostrum, removing fat to obtain defatted bovine colostrum, settling casein in the defatted bovine colostrum by an isoelectric point method or a chymosin method, obtaining whey liquid, or dissolving bovine colostrum whey powder to obtain the whey liquid, pre-filtering with a hollow fiber membrane or a ceramic membrane, removing the residual fat, casein and most thalli in the whey, conducting filtration sterilization with a polytetrafluoroethylene membrane filter, filling under a sterile condition, and obtaining the sterile bovine colostrum whey. The sterile bovine colostrum whey is degermed by a polytetrafluoroethylene degerming membrane based on pre-filtering, the handling capacity of feed liquid is increased, the service life of the membrane is prolonged, the membrane cost is saved, and the sterile bovine colostrum whey is suitable for industrial production. The content of immunoglobulin in the obtained whey is high; sterile requirements can be met; and the whey can directly serve as an immunoglobulin-rich whey product, and can also be added to beverages such as milk and fruit juice to be made into functional beverages.

The invention relates to a method for preparing an immunocompetent soybean peptide by enzymolysis and membrane separation, belonging to the technical field of soybean protein deep processing. The method comprises the following steps of: by taking defatted soybean protein powder as a raw material, firstly, carrying out acid cleaning on the defatted soybean protein powder to obtain acid washed soybean protein; then carrying out enzyme hydrolysis by using an Alcalase protease under certain conditions to obtain coarse soybean peptide liquid; clarifying the prepared coarse soybean peptide liquid by adopting microfiltration; then carrying out enrichment and desalination treatment by adopting ultrafiltration and nanofiltration; and finally, drying to obtain the powdery immunocompetent soybean peptide. The activity of the soybean peptide is determined by adopting a neutral red phagocytosis test and a lymphopoiesis method, which proves that the prepared soybean peptide has high immunocompetence.

The invention discloses a composite chitosan and cellulose membrane. A preparation method of the composite chitosan and cellulose membrane includes the steps of 1), precooling a cellulose solvent, adding cellulose into the precooled cellulose solvent with stirring, dissolving to obtain a cellulose solution, subjecting the obtained cellulose solution to defoaming, edulcoration, tape casting and film scraping, coagulating in coagulating bath to shape and performing water scrubbing so as to obtain a regenerated cellulose membrane; 2), dissolving chitosan in a solvent to enable the chitosan to be distributed uniformly; 3), coating a solution obtained in the step 2 on the regenerated cellulose membrane by a needle head in an electrostatic spraying manner so as to obtain the composite chitosan and cellulose membrane. The preparation method is simple, low in cost and free from toxicity and pollution, and the prepared composite chitosan and cellulose membrane is high in biocompatibility and biodegradability, good in anti-infection performance and blood coagulation effect, capable of promoting wound healing and enhancing immunocompetence and easy in functionalized expanding of application fields and can be widely applied to the fields of biological medicine, food industry and the like.

The invention relates to a pure Chinese herbal medicine egg-laying chicken feed additive, which is characterized by being prepared by crushing and mixing the following substances in parts by weight: 2-5 parts of radix codonopsitis, 2-4 parts of Angelica, 2-4 parts of Astragalus root, 1-3 parts of radix salviae miltiorrhizae, 2-5 parts of Chinese wolfberry, 2-5 parts of radix adenophorae, 4-6 parts of atractylodes rhizome, 2-5 parts of teasel root, 5-8 parts of pokeweed, 4-7 parts of motherwort, 1-3 parts of hemlock parsley, 5-8 parts of tuckahoe and 2-3 parts of kudzu root. The Chinese herbal medicines have nutritious and medicinal double actions, has a function of enhancing immunocompetence, can promote the ova growth development and nutrient substance digestion and absorption of egg-laying chicken, is free from residue or drug resistance, improves the laying rate, improves the protein content of eggs to 13.06% and keeps the cholesterol content of the eggs at a lower level.

The invention provides a human anti-vascular endothelial cell growth factor (VEGF) antibody as well as a coding gene and an application thereof. According to the invention, by virtue of genetic engineering means and a phage surface display technology, an anti-VEGF genetic engineering single-chain antibody is screened from a complete synthesis single-chain human antibody library, a variable region gene sequence of the anti-VEGF genetic engineering single-chain antibody is obtained, amino acids in a CDR (complementary determining region) of the anti-VEGF genetic engineering single-chain antibody are transformed by virtue of bioinformatics means, and finally a series of high-affinity anti-VEGF antibodies are obtained by constructing and screening mutation libraries and utilizing a chain exchange method, wherein affinity of the anti-VEGF antibodies with human VEGF is 10<-9>-10<-11>M. According to the invention, identification on immunocompetence and biological activity of the antibody is completed, the anti-VEGF antibody is verified to have the characteristic of being antagonistic to combination of VEGF and VEGFR2 and can effectively inhibit proliferation of vascular endothelial cells induced by VEGF. The invention provides a new specific candidate antibody molecule for resisting tumour and senile macular degeneration targeted by VEGF in future.

The invention belongs to the technical field of agriculture animal husbandry and food antitoxin and detoxication, and relates to mycotoxin adsorbent and a preparation method thereof. The mycotoxin adsorbent is composed of, by weight, 85-90% of montmorillonite, 5-7% of yeast cell walls, 1-3% of chitin, 1-2% of saccharomyces boulardii and 3-5% of natural plant extract in a mixing mode. The natural plant extract comprises tea tree oil, hesperidin, eugenol, citral, cinnamaldehyde and baicalein which are proportionally mixed. The mycotoxin adsorbent can be applied to fodder, aflatoxin B1 in the mycotoxin can be absorbed, other mold toxins such as zearalenone, ochratoxin, deoxynivalenol and fumitremorgin can also be effectively absorbed, the nutrient absorption rate of the fodder is low, meanwhile, breeding and balancing of probiotics in intestinal canals can be promoted, and the immunocompetence of animals is enhanced.

The invention relates to the technical field of aquaculture, and specifically relates to grass carp feed containing Chinese herbal medicines. The feed is prepared by the following raw materials by weight: 45-50 parts of corn, 20-22 parts of rye bran, 8-10 parts of fried sunflower seeds, 20-25 parts of soybean meal, 15-18 parts of peanut straws, 12-15 parts of wheat middlings, 3-4 parts of calcium hydrophosphate, 6-8 parts of watermelon peels, 5-6 parts of pumpkin seeds, 8-10 parts of potamogeton distinctus, 8-10 parts of corn tender leaves, 1-2 parts of radix scutellariae, 1-2 parts of radix isatidis, 1-2 parts of radix bupleuri, 0.5-1 part of rheum officinale, a proper amount of salt and 4-5 parts of a phagostimulant. In the prepared grass carp feed, plants such as watermelon peels and potamogeton distinctus that the grass carp likes are added to basic raw materials, thereby increasing palatability of the feed. The Chinese herbal medicines can enhance disease-resistant and bacterium-resistant capacities of the grass carp, so that use of chemical medicines is prevented. The grass carp fed by the feed has enhanced immunocompetence, effectively reduced morbidity, obviously increased in weight and is active in appetite.

The invention discloses a security system for a computer network. The security system comprises a host, a virtual switch, a core physical switch and a bypass firewall, wherein encryption modules are respectively arranged in the host and the virtual switch; an identity verification module is also arranged in the virtual switch; a key of each encryption module is updated once at each access; the security system further comprises a virus characteristic matching module, a port audit module, a traffic statistics module, a network anomaly assessment module, a virus isolation module, an emergency channel module, a restoration module and a data isolation uploading module, According to the security system, the network traffic is monitored and audited, so that the network is maintained in good condition; through analysis and memory of an unknown invasion behavior, the network immunocompetence is improved; the key is updated once at each access, so that the security of the network is further improved, and meanwhile, the system has data protection capability, so that a condition that a user is away from a computer to cause information loss is also avoided.