The invention discloses a preparation method of a pea
peptide with a prebiotics effect. The preparation method comprises the following steps of S1, uniformly agitating
pea protein with water accordingto a
solid-
liquid ratio of (1 to 5) to (1 to 20), so as to obtain a
mixed solution, and shifting to S2; S2, in a natural pH (potential of
Hydrogen) condition, at a temperature of 45 to 55 DEG C, adding alkali
protease into the
mixed solution, and carrying out enzymolysis for 0.5h to 1.5h; next, adding
neutral proteinase, and carrying out the enzymolysis for 1h to 3h; next, adding
papain, carryingout the enzymolysis for 1h to 3h, and shifting to S3; S3, raising the temperature to 90 to 100 DEG C, carrying out
enzyme deactivation, and shifting to S4; S4, filtering and collecting filtrate, carrying out vacuum concentration on the filtrate, and then carrying out spray-
drying, so as to obtain the pea
peptide. The invention also discloses application of the pea
peptide with the prebiotics effect, which is prepared and obtained through the method provided by the invention, to food. The pea peptide prepared by the preparation method has the prebiotics effect; the preparation method of the pea peptide does not need to artificially regulate a pH value; the operation is simplified; the preparation time is effectively shortened, and the production cost is decreased.