52 results about "Enteropathogenic bacteria" patented technology

Wastewater treatment systems and processes for: removal of solids, pathogens, nitrogen, and phosphorus from municipal and agricultural wastewater include nitrification of wastewater and increasing the pH of the nitrified wastewater by adding a metallic-containing salt and hydroxide to precipitate phosphorus to form a useable effluent having a specified nitrogen:phosphorus ratio that is useful as a fertilizer or spray for remediation of contaminated soils. The presence of infectious microorganism such as enteropathogenic bacteria and picarnoviruses will be reduced in the useable effluent. The precipitated phosphorus is recovered and used to form useable phosphorus products.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention discloses a safe compound feed of juvenile prawn of penaeus vannamei boone, which consists of bean pulp, pea albumen powder, fish powder, blood cell albumen powder, cuttlefish powder, shrimp shell powder, double-low rapeseed meal, cotton pulp, monocalcium phosphate, No. 1 flour, gluten powder, 50% choline chloride, complex vitamin for shrimp, microelement additive for shrimp, bacillus licheniformis, decorticating element, lower polyxylose, fermented peptide for aquatic product, gene recombinant protein growth promoter, nutrition immunology aquatic product food attractant, phospholipids oil and fish oil. With the gene recombinant protein growth promoter, the nutrition immunology aquatic product food attractant, and the high antibacterial peptide-containing fermented peptide, the safe compound feed can effectively restrain various enteropathogenic bacteria, improves the digestibility of the nutrition constituent of the feed, promotes the growth of the penaeus vannamei boone, can improve the organism immunity of the penaeus vannamei boone, and can obviously improve the survival rate of the penaeus vannamei boone.

Oligosaccharides produced by an alternansucrase enzyme catalyzed reaction of sucrose with an acceptor oligosaccharide are effective as prebiotics for controlling enteric bacterial pathogens. Populations of enteropathogenic bacteria may be substantially reduced or inhibited by treatment of an animal with a composition comprising one or more of these oligosaccharides in an amount effective to promote the growth of beneficial bacteria. The method is particularly effective for the control of Salmonella species, enteropathogenic Escherichia coli, and Clostridia perfringens.

The invention discloses a method for preparing mead with a fruit flavor. In the method, honey is used as a raw material, and the mead is prepared by two times of fermentation of flavor-producing yeasts, namely primary fermentation for producing wine and secondary fermentation for generating flavor. The alcohol volume of the mead prepared by the method is 6 to 6.5 percent and the reducing sugar concentration in the mead is 60 to 65g / L. The mead does not contain cane sugar, artificial essence, pigment and preservative. The mead tastes pure, slightly sweet and fresh and does not taste bitter. The mead has a pure banana flavor and wine fragrance and contains 17 amino acids and various vitamins. The mead is pure mead brewed wine with a fruit flavor. The result of a test performed on the product prepared by the method according to B2758-2005 brewed wine sanitation standards shows that the mead meets the requirements of national brewed wine sanitation standards in total sulfur dioxide (SO2) content, lead content, total number of bacterial colonies, number of coliform colonies, number of enteropathogenic bacteria and the like. The mead can be drunk and satisfies the demands of people for low-alcohol-volume nutritive wine.

The invention discloses a safe compound feed for crablets, comprising soya bean meal, cotton seed meal, rapeseed meal, peanut meal, fish meal, fermenting protein peptides for aquaculture, squid extracts, small shrimp meal, phospholipid oil, fish oil, calcium biphosphate, flour, unshelling growth-promoting powder, trace elements for crablets, compound vitamins for crablets, 50% choline chloride, gene recombinant proteins and nutrient immune type aquatic phagostimulant. The invention can effectively inhibit various enteropathogenic bacteria by utilizing a gene recombinant protein growth immune promoter, the nutrient immune type aquatic phagostimulant and the fermenting protein peptides with high antibacterial peptide content, improve the digestibility of the nutrient components of the safe compound feed, promotes the growth of the crablets, also enhance the organism immunity of the crablets and remarkably increase the survival rate of the crablets.

The invention discloses a Bacillus subtilis BSC16a and application thereof. The Bacillus subtilis is preserved in China General Microbiological Culture Collection Center on September 6, 2019, and thepreservation number is CGMCC NO: 18473. The strain has a good in-vitro probiotic property. The strain disclosed by the invention plays a good probiotic role in the aspects of improving the growth performance and body immunity of growing and fattening pigs and reducing enteropathogenic bacteria. In addition, the Bacillus subtilis BSC16a disclosed by the invention is used as a probiotic preparationfor feeding diarrhea young animals. The strain has a good prevention and treatment effect on diarrhea animals. The strain can also be used as a feed additive for preventing and treating diarrhea of piglets, lambs and calves.

The invention belongs to the technical field of microbes and particularly relates to lactobacillus plantarum GL-5 with oxidation resisting activity and application thereof. The lactobacillus plantarumGL-5 with oxidation resisting activity disclosed by the invention is screened out from a home-made fermented dairy product of a herdsman's house of a Qinghai Guoluo region with an altitude of 4,000mor more, is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on November, 21, 2019 and has an accession number of CGMCCNo. 18988. The lactobacillus plantarum GL-5 has a 16S rRNA sequence shown in SEQ ID NO: 1. The lactobacillus plantarum GL-5 provided by the invention has relatively good bacteriostatic activity, and activity of enteropathogenic bacteria, i.e., staphylococcus aureus and escherichia coli can be remarkably inhibited; the lactobacillus plantarum GL-5 provided by the invention has relatively high oxidation resisting activity and can remarkably tolerate catalase; and the lactobacillus plantarum GL-5 provided by the invention has relatively high gastrointestinal fluid tolerance and cholate tolerance,and the survival rate of the lactobacillus plantarum GL-5 after culture in simulated gastric fluid and intestinal fluid is remarkably higher than that of lactobacillus rhamnosus LGG.

The invention relates to an Enterococcus faecium strain producing bacteriocin. The strain was preserved as a name Enterococcus faecium R1 by China General Microbiological Culture Collection Center on January 20, 2016, with preservation number CGMCC No.12085. A source of the strain is farm traditional fermented soybean paste and is presented as a circular or elliptic white smooth nontransparent colony smaller than 1mm in an MRS agar culture medium; uniform turbid growth is presented in an MRS liquid culture medium; thalli are spherical under an electron microscope and are arranged in pairs or in a chain, and gram staining is positive. The strain provided by the invention is sensitive to antibiotics, does not contain virulence genes and is a safe strain. The bacteriocin produced by the strain is enterococcin P, is stable under acidic conditions, has a very good thermal stability and has a good bacteriostatic effect on common enteropathogenic bacteria.

The invention relates to compound orthophthalaldehyde disinfectant, and a preparation method and application thereof. The compound orthophthalaldehyde disinfectant is prepared from orthophthalaldehyde (1), tetrakis hydroxymethyl phosphonium sulfate (2), inositol phosphate (3), a penetrating agent (4), ampholyticsurfactant (5) and deionized water (6). The compound orthophthalaldehyde disinfectant is characterized by that each 1000ml compound orthophthalaldehyde disinfectant comprising 1.0 to 102.4g of the orthophthalaldehyde and 1.3 to 133.3g of inositol phosphate. The compound orthophthalaldehyde disinfectant can kill enteropathogenic bacteria, pyogenic cocci, hepatitis virus, avian influenza virus, various epidemic diseases, bacterial spore and the like, and is applicable to performing sterilization on articles in farms, environments, air, ground, water, epidemic focus, health and epidemic prevention and the like.

The invention discloses a no-wash hand sanitizer capable of killing viruses. The hand sanitizer is prepared from components in parts by weight as follows: 1-2 parts of hydrogen peroxide, 700-900 partsof absolute ethyl alcohol, 20-50 parts of propylene glycol, 10-20 parts of alkyl glycoside, 5-15 parts of cocamidopropyl betaine, 10-20 parts of glycerin, 1-10 parts of citric acid and 100-300 partsof purified water. The prepared no-wash hand sanitizer capable of killing viruses contains sanitizing ingredients, can kill enteropathogenic bacteria, pyogenic cocci, pathogenic saccharomyces cerevisiae and common hospital infection bacteria and is compounded from two or more main sanitizing ingredients, and a proper stabilizer and surfactant are added, so that the product is high in stability andsterilization effectiveness and good in sterilization effect.

The invention relates to creped prebiotic tissue products. The tissue products comprise prebiotics in sufficiently high amounts to have a prebiotic effect in use, without negatively affecting tissue product properties, such as tensile strength, stiffness or softness. Thus, in certain embodiments the present invention provides soft, durable creped prebiotic tissue products that are able to improve the growth of healthy bacteria such as Bifidobacterium spp. or Lactobacillus spp. without promoting growth of enteropathogenic bacteria. The tissue products provide these benefits yet have a GMT greater than about 500, a Stiffness Index less than about 20 and a TS7 value less than about 10.

The invention discloses a preparation method of a probiotics agent capable of lowering blood fat. Bacillus licheniformis BLc strains are activated and are subjected to scale-up culture by seed culture; the Bacillus licheniformis BLc strains subjected to scale-up culture are inoculated into a fermentation medium; NaCl, K2HPO4.3H2O and NaH2PO4 in the fermentation medium have actions on accelerating the secretion of various enzymes of the protease, the lipase, the amylase and the like of the BLc strains and on improving activity; the activity of the enzyme can be improved if the pH of the fermentation medium is controlled to 6-7, Enteromorpha is degraded, and a large quantity of Bacillus licheniformis BLc strain sporophyte is contained; after spray drying, the sporophyte temporarily looses activity; after being eaten, the sporophyte revives when entering the stomach and intestine and secretes various enzymes so as to accelerate gastrointestinal foods to decompose and decompose cholesterol, fat and the like; meanwhile, antagonism can be carried out on enteropathogenic bacteria; and the Enteromorpha degradation product has the action on lowering blood fat, resisting lipid peroxidation and preventing fatty liver. The invention provides the preparation method of probiotics agent capable of lowering blood fat, which has the advantages of low raw material cost and simple preparation method and has an action on accelerating the gastrointestinal function.

The invention discloses a method for detecting food-borne enteropathogenic bacteria O157: H7 based on nucleic acid aptamers, nanoparticles and quantum dot markers, which comprises the following steps: taking nucleic acid aptamer functionalized nano-enriched magnetic beads as a capture probe, capturing target bacteria existing in a reaction system, incubating, and recovering magnetic bead aptamer-target bacteria through a magnetic separator; then, adding nucleic acid aptamer functionalized quantum dots; and enriching magnetic beads under the action of a magnetic field, resuspending the precipitate in a buffer solution, determining the fluorescence intensity by using a fluorescence chemical analyzer, and determining the total bacterial count of the food-borne enteropathogenic bacteria O157: H7 according to the fluorescence intensity. According to the invention, the functionalized quantum dots are applied to detection of E.coli O157: H7 in food, and an efficient and rapid detection method of E.coli O157: H7 is finally established in combination with a nucleic acid aptamer functionalized magnetic bead enrichment method.

The invention relates to detecting microorganisms in specimens for the purposes of inter alia diagnoses, screenings, quarantine inspections, and clinical tests. Specifically, it relates to detecting and quantifying enteropathogenic bacteria in a fecal specimen, including Shigella species, Salmonella species, Campylobacter species, enterohemorrhagic E. coli or Verocytotoxin-producing E. coli, Vibrio cholerae, and Clostridium perfringens. Provided is a method for detecting a microorganism in a fecal specimen, wherein the method comprises preparing a 25-50% (wt / vol) fecal lysate, optionally followed by isolating a nucleic acid from the fecal lysate; subjecting the nucleic acid to a nucleic acid amplification assay using a set of at least two nucleic acid amplification primers specific for the microorganism and detecting amplified nucleic acid to determine the presence of the microorganism in the specimen.

The invention discloses a coarse cereal cookie which comprises, by weight, 30-50 parts of coarse rice powder, 30-40 parts of soybean meal, 30-40 parts of oat powder, 30-40 parts of corn flour, 15-25 parts of eggs, 10-14 parts of white granulated sugar, 2-4 parts of salt, 8-12 parts of tartary buckwheat, 8-10 parts of cassia seeds, 20-30 parts of konjak, 6-8 parts of hawthorn fruits, 40-60 parts of vegetable oil, 50-60 parts of honey and 50-60 parts of water. The coarse cereal cookie has rich nutrition, the added konjak has rich gulcomannan so as to smoothen defecation and soften excrement, pH in an enteric canal is lowered, and enteropathogenic bacteria are obviously restrained; the added tartary buckwheat and hawthorn fruits have rich nutrition and can prompt enteric digestion. Accordingly, the obtained coarse cereal cookie has rich nutrition, has no preservative, is good in taste and can clean the enteric canal and aid digestion.

The invention discloses a lactobacillus plantarum P101 with high antioxidant activity and application thereof, the lactobacillus plantarum P101 is separated from farmhouse homemade pickled Chinese cabbages in Jiyan, Jiangxi Province, the 16S rRNA sequence of the lactobacillus plantarum P101 is shown as SEQ ID NO: 1, and the preservation number is CCTCC M 2021108. The lactobacillus plantarum P101 disclosed by the invention has good antibacterial activity and can be used for remarkably inhibiting the activity of enteropathogenic bacteria such as staphylococcus aureus and pseudomonas aeruginosa; the lactobacillus plantarum P101 has high antioxidant activity, in-vitro experimental results show that the clearance rate of the lactobacillus plantarum P101 to 1, 1 diphenyl-2-trinitrophenylhydrazine (DPPH) and hydroxyl radicals is remarkably higher than that of lactobacillus rhamnosus GG (LGG), and in-vivo experimental results show that the lactobacillus plantarum P101 can effectively weaken oxidative stress injury induced by alcohol and inhibit inflammatory response of the organism. The survival rate of the lactobacillus plantarum P101 in an acid or high cholate environment is obviously higher than that of lactobacillus plantarum ZDY2013, and in addition, the lactobacillus plantarum P101 has outstanding benefits in relieving fatty liver hepatitis induced by alcohol.

The invention provides a medical instrument rapid sterilizing agent which comprises the following components in percentage by weight: 0.1-2% of sodium dichloroisocyanurate, 5-15% of sodium silicate, 2-10% of sodium phosphate, 0.5-5% of boron nitride, 1-5% of complexing agent, 0.5-2% of corrosion inhibitor, 0.2-1% of enhancer, 0.1-2.5% of stabilizer, 0.1-2.55% of penetrant and the balance of water.The invention also provides a preparation method of the medical instrument rapid sterilizing agent, which comprises the following steps: heating and mixing the sodium dichloroisocyanurate, sodium silicate, sodium phosphate and boron nitride at 60-70 DEG C for 1-3 hours, cooling, adding the stabilizer, the penetrant, EDTA-2Na, pentaerythritol ester and sodium metasilicate, adding water to 100%, and uniformly mixing to obtain the sterilizing agent. The medical instrument can be sterilized with a stock solution within 30 minutes, and can be disinfected within 5 minutes. The medical instrument rapid sterilizing agent disclosed by the invention can be used for killing enteropathogenic bacteria, pyogenic coccus, pathogenic saccharomycetes, bacterial spores, aspergillus niger and mycobacterium tortoise, can be used for inactivating viruses, and has the advantages of high efficiency, quick effect, safety, simplicity, convenience and no corrosion.

The invention relates to a solid-liquid combined peracetic acid disinfectant and a preparation method thereof, relates to the technical field of disinfectant and venom, and is mainly used for disinfection and sterilization of soft endoscopes. After A and B are mixed, dissolved and diluted, sterilization can be achieved within 10 min, and disinfection can be achieved within 5 min. Enteropathogenic bacteria, pyogenic cocci, pathogenic saccharomycetes, bacterial spores, aspergillus niger and tortoise mycobacteria can be killed, and viruses can be inactivated. The pesticide has the advantages of high efficiency, broad spectrum, quick effect, good safety, simple and fast use, and no corrosion. Particularly, the agent B in the composition is solid powder, and the agent A and the agent B are mixed and dissolved to form a concentrated solution which can be diluted by 6 times for use, so that the logistics cost is saved. The device is suitable for high-level disinfection and sterilization of various medical instruments, endoscopes and the like in medical and public institutions.

The invention discloses swine-borne lactobacillus plantarum and application thereof. The strain is named lactobacillus plantarum R-21(Lactobacillus plantarum R-21), is preserved in the China Center for Type Culture Collection, has the preservation number of CCTCC M2018009, and is preserved on January 8, 2018. The invention further discloses application of the strain in preparation of feed or silage. The lactobacillus plantarum grows quickly and has strong acid producing performance; and the fermentation broth of the strain has a remarkable inhibiting effect on aspergillus and common enteropathogenic bacteria and can be used in feed addition or fermented feed preparation.

The invention relates to the technical field of inhibiting and killing poultry enteropathogenic bacteria, and discloses a preparation method of probiotic soluble solid powder for inhibiting and killing poultry enteropathogenic bacteria. The preparation method comprises the following preparation steps: 1) taking the following substances in percentage by weight: 13%-17% of soluble bacterial powder, 9%-11% of short-chain fatty acid (salt) and glyceride thereof, 19%-22% of inactivated lactobacillus acidophilus soluble powder, 2%-4% of bifidobacterium, 48%-53% of soluble carrier, 1%-3% of oligosaccharide and 0.5%-1% of trace elements; (2) stirring the substances in a stirring drum to form mixed powder; (3) shaking the mixed powder in a sealer to form probiotic soluble solid powder; stirring and mixing according to a weight ratio in a specific range to form mixed powder, and uniformly shaking to form probiotic soluble solid powder; the probiotic soluble solid powder is soluble powder aiming at the intestinal health of poultry, and probiotics are stored in a solid form, so that the number of spores of the probiotics is increased, the probiotics are easily dissolved in water, and the effect is improved.

The invention discloses a quadruple PCR detection primer group for simultaneously detecting shigella, salmonella, clostridium welchii and escherichia coli, which comprises four pairs of primers, namely a shigella upstream primer pair and a shigella downstream primer pair which are respectively SEQIDNO: 1 and SEQIDNO: 2; an upstream primer pair and a downstream primer pair of salmonella are respectively SEQ ID NO: 3 and SEQ ID NO: 4; the clostridium welchii A type primer pair is shown as SEQ ID NO: 5 and SEQ ID NO: 6 respectively; and the primer pairs of the escherichia coli are respectively SEQ ID NO: 7 and SEQ ID NO: 8. A multiple detection method is established for common enteropathogenic bacteria in chicken farms, on the basis of high detection accuracy and good specificity, the detection time is shortened, the detection efficiency is improved, and the multiple detection method is more beneficial to application in large-scale breeding production.

The invention discloses an antibiotic-free feed additive for preventing and controlling calf diarrhea as well as a preparation method and a use method of the antibiotic-free feed additive, and relates to the antibiotic-free feed additive for preventing and controlling the calf diarrhea as well as the use method of the antibiotic-free feed additive. The invention aims to solve the technical problems that in the current prevention and control of calf diarrhea, the drug resistance of bacteria is gradually increased, beneficial microorganisms in digestive tracts can be inhibited or killed by using antibiotics, intestinal flora imbalance is caused, the resistance of calves is reduced, visceral organs such as livers and kidneys are damaged, and the illness state is aggravated. The sodium humate and the attapulgite powder have high adsorption capacity, and can effectively inhibit reproduction of enteropathogenic bacteria and adsorb toxins generated by the enteropathogenic bacteria; glutamine provides energy for proliferation and development of intestinal epithelial cells; and the xylooligosaccharide and the probiotics can regulate the intestinal microenvironment, improve the intestinal flora, increase the intestinal probiotics and reduce the number of intestinal pathogenic bacteria. Therefore, the product can effectively prevent and control calf diarrhea by improving the physical barrier and the biological barrier of the calf intestinal tract to protect the intestinal tract health.

The invention relates to the technical field of feed additives, in particular to a preparation method of a biological agent for improving the immune function of animal bodies. The method comprises the following steps: activating a clostridium butyricum strain; transferring the activated clostridium butyricum strain into a fermentation tank filled with a seed culture medium, culturing at 32-34 DEG C for 18-20 hours to obtain a first-stage seed solution, then transferring the first-stage seed solution into a seed tank filled with a fermentation culture medium, and culturing at 32-34 DEG C for 18-20 hours to obtain a second-stage seed solution; transferring the second-stage seed solution into a fermentation tank filled with a fermentation culture medium according to the inoculum size of 8% to obtain a culture solution, and adding a pH regulator in the culture process to obtain a fermentation solution when the spore formation rate is 10% or above through microscopic examination; concentrating the fermentation solution; and freezing and drying. The clostridium butyricum biological agent can regulate intestinal microecological balance, inhibit growth of enteropathogenic bacteria such as escherichia coli, salmonella and shigella, enhance organism immunity and promote weight gain of animals.

The invention belongs to the technical field of daily disinfection products, and particularly relates to a preparation method of a skin mucous membrane disinfectant. The invention provides a preparation method of a disinfection product which is better than existing formulas in the prior art. Through detection of authoritative departments, the disinfectant has an inactivation effect on enteropathogenic bacteria such as Escherichia coli, suppurative bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa, and pathogenic fungi such as Candida albicans. The disinfectant not only has a good disinfection effect, but also has no irritation to skin and mucosa, is safe, reliable, practical and convenient, and is suitable for disinfection of skin and mucosa, such as disinfection of pre-surgical hands, surgical part skin, injection part skin and sanitary hands and disinfection of oral mucosa, wounds, burns and vaginal skin mucosa. The skin mucous membrane disinfectant provided by the invention is reasonable in formula, reliable in preparation process, excellent in performance and convenient to use, and has a wide market prospect.

The invention discloses a bacillus licheniformis extracellular polymeric substance organic selenium product as well as a preparation method and application thereof. The bacillus licheniformis extracellular polymeric substance organic selenium product is prepared from a bacillus licheniformis extracellular polymeric substance and sodium selenite. In the bacillus licheniformis extracellular polymeric substance organic selenium product, the content of organic selenium is 20-150mg / g. The invention provides the bacillus licheniformis extracellular polymeric substance organic selenium which is simple in production process, mild in condition, safe and non-toxic, is in a nanosphere shape, not only can safely supplement the selenium content of animal bodies, but also has the functions of resisting oxidation and inhibiting enteropathogenic bacteria.

The invention belongs to the technical field of molecular biology, and particularly relates to a primer probe group, kit and detection method for multiple detection of enteropathogenic bacteria based on a fluorescent RMA method. The primer probe group comprises a primer and a probe for detecting salmonella, a primer and a probe for detecting shigella, and a primer and a probe for detecting vibrio parahaemolyticus.

The invention provides bacillus coagulans with high lactic acid yield, biological fermentation feed as well as a preparation method and application of the biological fermentation feed. The preservation number of the bacillus coagulans is CGMCC No.21669. The biological fermentation feed preferably comprises bacillus subtilis, saccharomyces cerevisiae and a fermentation product of the bacillus coagulans. The biological fermentation feed provided by the invention contains rich small peptides, acidic substances, dietary fibers and other active ingredients, can emphatically improve the intestinal health of animals, improve the palatability and production performance of livestock, poultry and aquatic animals, reduce animal diarrhea, improve the product quality, kill enteropathogenic bacteria and reduce environmental pollution, has the advantages of being safe, non-toxic and free of residues and can replace antibiotics to a certain extent, and the preparation method is simple and feasible.