The invention provides a Chinese herbal medicine antibiotic hand washing and a preparation method thereof and relates to detergent field. The Chinese herbal medicine antibiotic hand washing is made of the mixture of Chinese herb liquorice, peony bark, camellia fruit extract liquid and synergistic agent and eradicator and can decontaminate and disinfect under the condition that the actual washing of the customers are met. The Chinese herbal medicine antibiotic hand washing is prepared by APG, AEO-9, AEO-7, BS-12, AES, peony bark extractive matter, liquorice extractive matter and camellia fruit extractive matter, etc. The preparation method is that deionized water and the materials are added to a dissolution kettle for dissolution, then obtaining the product. The invention adopts Chinese herbal extracts, surfactant and other effective components for optimization and combination, enhances sterilization effect, functions to decontaminate, moisten and protect hands and has low production cost.