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Biological agent for improving immune function of animal bodies and preparation method thereof

A biological preparation and immune technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as reducing animal immune function, destroying microecological balance, and digestive dysfunction, and enhancing immunity of the body. , The effect of promoting animal weight gain and reducing consumption

Pending Publication Date: 2021-05-04
湖北绿天地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, a variety of antibiotics and hormones have been widely used as feed additives. Although they have played an indispensable role in livestock and poultry production, disease prevention and promotion of animal growth, due to long-term use and abuse, the use of antibiotics There are more and more problems in the use, mainly manifested in: drug residues and toxicity; large-scale use of broad-spectrum antibiotics in feeding livestock leads to rapid drug resistance of bacteria; long-term use of antibiotics disrupts the mutual restriction of microbial communities in the microbial system of livestock and poultry , and then destroy the micro-ecological balance in the body, leading to digestive disorders, causing various digestive tract diseases; reducing the immune function of animals

Method used

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  • Biological agent for improving immune function of animal bodies and preparation method thereof
  • Biological agent for improving immune function of animal bodies and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method for a biological agent for improving the immune function of an animal body, comprising the following steps:

[0030] (1) Activation treatment of Clostridium butyricum strains: insert the Clostridium butyricum strains into the seed culture medium, and cultivate and grow them in a constant temperature incubator at 32-34°C for 18-20 hours to realize Clostridium butyricum Activation treatment of bacteria;

[0031] (2) Transfer the activated Clostridium butyricum strains to a fermenter equipped with a seed medium according to an 8% inoculum size and cultivate them at 32 to 34°C for 18 to 20 hours to reach the logarithmic growth phase, Thereby obtain the primary seed liquid; then transfer the primary seed liquid to the fermenter equipped with the seed medium according to the inoculation amount of 8%, and cultivate it at 32-34° C. for another 18-20 h to reach the logarithmic growth phase, Thereby obtaining the secondary seed liquid, the culture endpoints ...

Embodiment 2

[0038] The preparation method of the biological agent for improving the immune function of the animal body in this example is the same as that of Example 1, except that the seed culture medium includes 15g / L of glucose, 10g / L of peptone, 5g / L of beef Cream, the agar of 15g / L, the yeast powder of 3g / L, the potassium dihydrogen phosphate of 0.6g / L and the calcium carbonate of 0.3g / L, the pH value of described seed culture medium is 7.1 to 7.2; The fermentation culture The base includes 25g / L cornstarch, 6g / L yeast extract, 3g / L peptone, 2g / L beef extract, 0.5g / L potassium dihydrogen phosphate, 1g / L sodium bicarbonate, 0.2g / L The manganese sulfate of L and the calcium chloride of 0.1g / L, the pH value of described fermentation medium is 7.1 to 7.2.

Embodiment 3

[0040] The preparation method of the biological agent for improving the immune function of the animal body in this example is the same as that of Example 1, except that the seed culture medium includes 13g / L of glucose, 9g / L of peptone, and 4g / L of beef Cream, the agar of 14g / L, the yeast powder of 2g / L, the potassium dihydrogen phosphate of 0.5g / L and the calcium carbonate of 0.25g / L, the pH value of described seed medium is 7.1 to 7.2; The fermentation culture The base includes 23g / L cornstarch, 5g / L yeast extract, 2g / L peptone, 1.8g / L beef extract, 0.4g / L potassium dihydrogen phosphate, 0.7g / L sodium bicarbonate, 0.15 The manganese sulfate of g / L and the calcium chloride of 0.06g / L, the pH value of described fermentation medium is 7.1 to 7.2.

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Abstract

The invention relates to the technical field of feed additives, in particular to a preparation method of a biological agent for improving the immune function of animal bodies. The method comprises the following steps: activating a clostridium butyricum strain; transferring the activated clostridium butyricum strain into a fermentation tank filled with a seed culture medium, culturing at 32-34 DEG C for 18-20 hours to obtain a first-stage seed solution, then transferring the first-stage seed solution into a seed tank filled with a fermentation culture medium, and culturing at 32-34 DEG C for 18-20 hours to obtain a second-stage seed solution; transferring the second-stage seed solution into a fermentation tank filled with a fermentation culture medium according to the inoculum size of 8% to obtain a culture solution, and adding a pH regulator in the culture process to obtain a fermentation solution when the spore formation rate is 10% or above through microscopic examination; concentrating the fermentation solution; and freezing and drying. The clostridium butyricum biological agent can regulate intestinal microecological balance, inhibit growth of enteropathogenic bacteria such as escherichia coli, salmonella and shigella, enhance organism immunity and promote weight gain of animals.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a biological preparation and a preparation method for improving the immune function of an animal body. Background technique [0002] Since the 20th century, a variety of antibiotics and hormones have been widely used as feed additives. Although they have played an indispensable role in livestock and poultry production, disease prevention and promotion of animal growth, due to long-term use and abuse, the use of antibiotics There are more and more problems in the use, mainly manifested in: drug residues and toxicity; large-scale use of broad-spectrum antibiotics in feeding livestock leads to rapid drug resistance of bacteria; long-term use of antibiotics disrupts the mutual restriction of microbial communities in the microbial system of livestock and poultry , and then destroy the micro-ecological balance in the body, leading to digestive disorders, causing various digestiv...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/12C12N1/20C12N1/04C12R1/145
CPCA23K10/18A23K10/12C12N1/20C12N1/04
Inventor 林林易秋萍宋艳芬刘畅万顺孙玲
Owner 湖北绿天地生物科技有限公司
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