126 results about "Clostridium sp" patented technology

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I):wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R<1>, R<2>, R<3>, and R<4 >are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

To provide a series of techniques capable of producing isoprene from syngas or the like.Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.

The present invention relates to recombinant fragments of C. difficile TcdA and TcdB that may be used in the development of vaccines against C. difficile associated disease. More particularly it relates to combinations comprising a ToxB-GT antigen and a TcdA antigen or a ToxA-GT antigen and a TcdB antigen.

Aiming at the problems of polyethylene terephthalate (PET) plastic degradation in the prior art, the invention provides a novel PET degradation biocatalyst and a construction method thereof. The PET degradation whole-bacterium catalyst is obtained by expressing PET enzyme in a heat-resistant strain; the PET enzyme has a sequence of SEQ NO.1 or a sequence with similarity of 99% with the sequence ofSEQ NO.1; and the heat-resistant strain is clostridium thermofibrillae. The construction method of the PET degradation whole-bacterium catalyst comprises the following steps: (1) expressing PET degradation enzyme by a plasmid; (2) expressing PET degradation enzyme by a genome; and (3) expressing PET degradation bodies. The PET degradation whole-bacterium catalyst overcomes the problem of feedbackinhibition, not only has degradation efficiency obviously higher than a known whole-bacterium biodegradation system, but also has a culture condition free of aeration and stirring as an anaerobic microorganism, so that process cost is obviously reduced. In addition, simultaneous degradation of fibers and PET in a blended fabric is realized through a one-pot method; the reaction temperature is low, early-stage separation of fibers is not needed, and an additional carbon source is not needed in a degradation process, so that the PET degradation whole-bacterium catalyst has remarkable economy and efficiency.

Disclosed are culture media, protocols and kits for diagnosis of toxigenic Clostridium difficile, where the culture medium comprises Cooked Meat Medium with glucose; yeast extract; taurocholate; cycloserine; and cefoxitin.

The invention relates to a bacillus and application thereof. The bacillus coagulans is screened from intestinal contents of healthy piglets, the preservation number of the bacillus coagulans is CGMCC 22951, and the bacillus coagulans can produce acid, amylase and protease, is high in gastric acid resistance and cholate resistance and good in thermal stability, and can grow rapidly at the intestinal temperature of -39 DEG C of livestock and poultry. The invention further provides an application of the bacillus in facultative co-fermentation with clostridium butyricum. Through a high-temperature (39 DEG C) co-fermentation system, the cooling cost is reduced on one hand, and the growth of impurities are effectively inhibited on the other hand, so that the fermentation process is simplified; the newly screened strains grow faster under the facultative oxygen condition, so that the viable bacterium concentration and the spore concentration of the clostridium butyricum in a co-fermentation system are higher, and a new energy-saving and environment-friendly mode is provided for production of the clostridium butyricum.

The invention provides a microbe used for degrading deoxynivalenol, which belongs to microbe and feed additive fields. The microbe for degrading deoxynivalenol is obtained from the content in animal intestinal tract by pressure selection, gradient dilution and separation as well as screening, and is clostridium sp. by identification. The stability of the strain for degrading deoxynivalenol is good, in a L10 medium, the subcultring is carried out for 8-12 generations, the degradedation rate of DON is 100%. In the L10 medium, the initial concentration of DON is 25-100 ppm, the pH value is 5.0-8.0, anaerobic cultivation is carried out for 48-96 hours at the temperature of 30-40 DEG C, and the degradation rate of DON is more than 90%.

The invention discloses a preparation method and application of a feed additive, and belongs to the field of feed additives. The preparation method of the feed additive comprises the following steps of S1, treating the main materials; S2, carrying out aerobic fermentation: (1) feeding the main material into a tank; (2) adding auxiliary materials; (3) inoculating bacteria A and bacteria B, whereinthe bacteria A are aspergillus oryzae spore powder, and the bacteria B are saccharomyces cerevisiae; (4) performing turning and throwing; (5) inoculating bacteria C and bacteria D, wherein the bacteria C are lactobacillus casei, and the bacteria D are clostridium butyricum; (6) inoculating bacteria D; S3, carrying out anaerobic fermentation; S4, taking out of a pool for modulation; and S5, performing packaging and delivery inspection. The feed additive prepared by the invention is obtained by asynchronous fermentation of multi-strain combination and control of certain fermentation conditions,not only has multiple functions of food calling, immunity improvement, feed conversion ratio reduction, egg laying rate improvement and the like, but also can be simultaneously applied to feeding of animals such as poultry, livestock, aquatic products and the like, and is a multifunctional, healthy and safe feed additive.