128 results about "Clostridium sp" patented technology

The invention discloses high levels of receptors for Clostridium perfringens enterotoxin (CPE) have been found in ovarian cancer and uterine cancer tissue samples. In addition, successful in vivo treatment of a mouse model of ovarian cancer with intraperitoneal injection of CPE is disclosed. High levels of Ep-CAM protein is also disclosed in ovarian cancer tissue samples. Thus, the invention provides a method of treating ovarian cancer and uterine cancer by administering CPE. The invention also provides a method of treating cancer in a mammal involving intraperitoneal administration of CPE, where at least some cancerous cells are located in or adjacent to the peritoneal cavity of the mammal. The invention also provides a method of treating ovarian cancer involving administering an anti-Ep-CAM antibody. The invention also provides a method of treating cancers expressing claudin-3 or claudin-4 by administering an antibody against claudin-3 and / or an antibody against claudin-4. The invention also provides a method of protecting a mammal from CPE toxicity involving administering a protective agent that binds to claudin-3 and / or claudin-4 and inhibits CPE binding to claudin-3 and / or claudin-4.

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.

The invention relates to a solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans, wherein a production method is a solid state fermental cultivation method of the mixed bacteria. A solid state fermentation culture medium is formed with enzymolysis bean pulp and rice bran as major ingredients and added with proper amount of an inorganic salt; the solid state fermentation culture medium is subpackaged and then sterilized; next, the solid state fermentation culture medium is inoculated with an isometric clostridium butyricum TK2 seed solution accounting for 10% of the total weight of the solid state culture medium and an isometric bacillus coagulans TQ33 seed solution accounting for 5% of the total weight of the solid state culture medium, and stirred evenly for fermentation. After the fermentation is carried out at 37 DEG C for 48 hours, a compound probiotics preparation of the clostridium butyricum TK2 and the bacillus coagulans TQ33 is obtained by drying at 50 DEG C, crushing and sieving; in the fermentation of the mixed bacteria, antinutritional factors in the bean pulp are decomposed more effectively under the mutualistic synergistic effect of the clostridium butyricum and the bacillus coagulans; as a result, nutrient substances in the fermentation bean pulp are richer and more balanced; therefore, a high protein feed rich in compound probiotics is provided.

The invention relates to preparation and application of a diarrhoea-resistant type special composite microecological preparation for piggies, belongs to the field of a feed additive, solves the technical problems of the special composite microecological preparation prepared from various beneficial microorganisms and having favorable biology diarrhoea-resistant effects, for the piggies, the product strain compatibility and the feeding effect stability, and relates to the diarrhoea-resistant type special composite microecological preparation for piggies. The diarrhoea-resistant type special composite microecological preparation is characterized by being formed by combining bacterium powder I, with bacterium powder II and a carrier, wherein the bacterium powder I is coated composite lactic acid bacteria consisting of coated enterococcus faecalis and pediococcus acidilactici; the bacterium powder II is composite bacillus formed by mixed fermentation of three probiotics of bacillus subtilis, bacillus coagulans and clostridium butyricum. Various beneficial microorganisms in the diarrhoea-resistant type special composite microecological preparation for piggies have the characteristics of being high in bacterium restraining capacity, rapid in propagation, capable of realizing synergistic symbiosis, rich in produced enzymes and high in enzyme activity. A technique of united fermentation of various bacterium strains and strain compatibility is adopted for preparing the diarrhoea-resistant type special composite microecological preparation, so that the diarrhoea-resistant type special composite microecological preparation is worth of wide popularization and application.

A novel subtype of type A botulinum neurotoxin (BoNT / A) is disclosed in the application. Methods to purify the neurotoxin as well as uses thereof are also disclosed.

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I):wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R<1>, R<2>, R<3>, and R<4 >are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

The invention relates to a microbial feed additive and belongs to the field of an agricultural microbial feed additive. The microbial feed additive is prepared by compounding three microbes, namely bacillus subtilis, clostridium butyricum and saccharomyces cerevisiae according to a certain ratio, wherein bacillus subtilis and clostridium butyricum adopt liquid fermentation and saccharomyces cerevisiae adopts liquid-solid fermentation. As for solid culture, solid fermentation of a culture medium prepared by using lucid ganoderma cultural residue, wheat bran and the like as raw materials is carried out. The microbial feed additive is used for livestock breeding, can effectively raise immunologic functions such as disease resistance, disease prevention and the like of piglets and chicks and reduce death rate, and can be used in laying hens to enhance egg yield and improve egg quality. The microbial feed additive is of great significance for promoting sustainable development of poultry breeding industry and guaranteeing product quality and safety.

The invention discloses a preparation method and application of a fermented feed additive and belongs to the field of animal feed. The additive is prepared by a liquid-solid two-step fermentation method. The liquid-solid two-step fermentation method comprises the steps: firstly, performing liquid fermentation on probiotics of bacillus subtilis, lactobacillus acidophilus, clostridium butyricum, saccharomyces cerevisiae and saccharomyces boulardii; performing solid mixed fermentation for 5 to 7 days with solid raw materials after fermentation is finished; drying and packaging; then compounding acompound premix and compound enzyme to obtain a finished product. According to the fermented feed additive, corn bran, corn steep liquor, corn starch candy pomace, corn protein powder and the like are utilized as main raw materials; thus, the fermented feed additive has comprehensive nutrition, and a lot of active matters can be further generated after microbial fermentation. When the fermented feed additive is applied to laying hen breeding, organism immunity of the laying hens is improved, and disease resistance of the laying hens is enhanced; furthermore, the laying rate and egg quality ofthe laying hens can be improved. The raw materials of the fermented feed additive has wide source and is low in cost, no three wastes is generated in a production process, and environmental friendliness and safety are achieved. Thus, the fermented feed additive disclosed by the invention has better market application value.

The invention discloses a compound probiotic preparation for preventing and treating piglet diarrhea and a preparation method and application. The compound probiotic preparation is prepared from clostridium butyricum, lactobacillus reuteri and bifidobacterium longum, wherein the total quantity of viable bacteria in the compound probiotic preparation is 10<1> to 10<20> per ml. The compound probiotic preparation disclosed by the invention has the advantages that the diarrhea rate and the death rate of weaned piglets are remarkably reduced. On the sixth to twentieth day of the age in days of the piglets, the prepared compound probiotic preparation is irrigated into stomachs of 66 large binary hybrid piglets, the irrigation dosage is 5 to 10ml / time, and the piglets are irrigated once every day. Compared with a control group irrigated by a Phosphate Buffer Solution (PBS), the compound probiotic preparation has the advantages that the diarrhea rate of the group irrigated by the compound probiotic preparation at the first to fifteenth day stage after weaning (21 days of age) is remarkably reduced by 87 percent, and the death rate is reduced by 70 percent. The compound probiotic preparation effectively prevents the piglet diarrhea.

The invention belongs to the technical field of microorganisms, and particularly relates to a new rumen clostridium CPC-11(Clostridium sp.CPC-11) and application thereof. The new rumen clostridium Clostridium sp.CPC-11 is capable of converting lactic acid into hexanoic acid, using lactic acid or yellow water for producing hexanoic acid through fermentation, and being used in intensified culture of artificial pit mud or the process of increasing hexanoic acid and reducing lactic acid of pit fermentation, the yield of hexanoic acid is increased, and the rumen clostridium has excellent application prospects.

The present invention relates to recombinant fragments of C. difficile TcdA and TcdB that may be used in the development of vaccines against C. difficile associated disease. More particularly it relates to combinations comprising a ToxB-GT antigen and a TcdA antigen or a ToxA-GT antigen and a TcdB antigen.

The invention discloses the novel application of clostridium butyricum to drugs, in particular to the application of the clostridium butyricum to the preparation of a drug combination used for preventing and treating intestinal cancer. The novel application comprises the prevention of chronic intestinal disease canceration such as intestinal polyp, Crohn disease, ulcerative colitis, etc., the adjuvant therapy on advanced inoperable intestinal cancer, the adjuvant therapy after the intestinal cancer operation, etc.; the clostridium butyricum is selected from (but is not limited to) clostridium butyricum, CGMCC, and storage serial number 0313.1. In the forms of live bacteria and pharmic receivable different carriers, the clostridium butyricum is made into tablets, capsules, powders, liquid preparations, suppositories, and enemas; the clostridium butyricum can be used for preventing and treating the intestinal cancer for a long time through the methods of oral administration, clysis, injection in the tumor tissue; therefore, the life of a patient suffering from the intestinal cancer is prolonged, and the life quality of the patient suffering from the intestinal cancer is increased.

The invention provides a composite probiotic product formula and a production technology thereof, and relates to the field of feed additives. The composite probiotic product formula consists of the following raw materials in percentage by weight: 5-10% of enterococcus faecalis, 1-2% of bacillus subtilis, 1-2% of bacillus licheniformis, 2-4% of clostridium butyricum, 5-10% of saccharomyces cerevisiae, 5-20% of fructo-oligosaccharide and the balance of glucose. The production technology disclosed by the invention is simple, the specific and non-specific immunity of animal organisms can be effectively improved, the disease resistance of the organisms can be strengthened, the dosage during a breeding period is reduced, and the drug residues are reduced; balance of gastrointestinal bacterial flora of animals is adjusted, comprehensive absorption of nutrients is facilitated, and the breeding cost such as the feed conversion ratio and the feed-to-egg ratio is reduced; the breeding environment is improved, harmful gas such as ammonia is reduced, mycotoxin is removed, and damage caused by mildew and rot of the feed is reduced; and the quality of animal products is improved, and the breeding benefits are increased.

To provide a series of techniques capable of producing isoprene from syngas or the like.Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.

The invention discloses a method for regulating and controlling synthesis of an extracellular polymeric substance of clostridium acetobutylicum. The method is characterized in that an intron sequence is interpolated into a gene Spo0A of the clostridium acetobutylicum by using a type II intron gene knockout technology. The invention provides a simple and efficient method for regulating and controlling EPS (Extracellular Polymeric Substance) synthesis; a recombinant strain obtained based on the method is relatively good in regulating and controlling effect on the EPS; in case of taking glucose as a carbon source, when cultivated in an anaerobic bottle with the volume of 100 ml by using a slide biomembrane cultivation method, a clostisdium acetobutylicum biomembrane is 5.81 mum in thickness, and the thickness of a strain biomembrane cultivated under the same condition is 24.6 mum.

Aiming at the problems of polyethylene terephthalate (PET) plastic degradation in the prior art, the invention provides a novel PET degradation biocatalyst and a construction method thereof. The PET degradation whole-bacterium catalyst is obtained by expressing PET enzyme in a heat-resistant strain; the PET enzyme has a sequence of SEQ NO.1 or a sequence with similarity of 99% with the sequence ofSEQ NO.1; and the heat-resistant strain is clostridium thermofibrillae. The construction method of the PET degradation whole-bacterium catalyst comprises the following steps: (1) expressing PET degradation enzyme by a plasmid; (2) expressing PET degradation enzyme by a genome; and (3) expressing PET degradation bodies. The PET degradation whole-bacterium catalyst overcomes the problem of feedbackinhibition, not only has degradation efficiency obviously higher than a known whole-bacterium biodegradation system, but also has a culture condition free of aeration and stirring as an anaerobic microorganism, so that process cost is obviously reduced. In addition, simultaneous degradation of fibers and PET in a blended fabric is realized through a one-pot method; the reaction temperature is low, early-stage separation of fibers is not needed, and an additional carbon source is not needed in a degradation process, so that the PET degradation whole-bacterium catalyst has remarkable economy and efficiency.

Disclosed are culture media, protocols and kits for diagnosis of toxigenic Clostridium difficile, where the culture medium comprises Cooked Meat Medium with glucose; yeast extract; taurocholate; cycloserine; and cefoxitin.

The present invention relates to novel polypeptides derived from endolysins from a bacteriophage of Clostridium perfringens and to nucleic acid molecules encoding the same, as well as compositions thereof. The invention also provides uses of such polypeptides and nucleic acid molecules in the diagnosis and treatment of conditions and diseases associated with microbial cells such as Clostridium perfringens. In particular, the invention provides a polypeptide having endolysin activity derived from bacteriophage ΦCP51 of Clostridium perfringens and uses thereof.

The invention discloses a building method of an ulcerative colitis transformation animal model. By using the method, an inflammatory transformation model combining with clostridium difficile in a DSSacute colitis model is built; and different bacterium feeding modes, including three measures of intraperitoneal injection, gastric perfusion and enema are used. The model has the advantages that theproportion of mouse mesenteric lymphocytes Th17 can be obviously increased by the model; the secretion of IL-17 is increased; the result is more similar to the pathological features of clinic patientswith ulcerative colitis; and the clostridium difficile feeding effect on the mice through gastric perfusion is best.

The invention relates to a bacillus and application thereof. The bacillus coagulans is screened from intestinal contents of healthy piglets, the preservation number of the bacillus coagulans is CGMCC 22951, and the bacillus coagulans can produce acid, amylase and protease, is high in gastric acid resistance and cholate resistance and good in thermal stability, and can grow rapidly at the intestinal temperature of -39 DEG C of livestock and poultry. The invention further provides an application of the bacillus in facultative co-fermentation with clostridium butyricum. Through a high-temperature (39 DEG C) co-fermentation system, the cooling cost is reduced on one hand, and the growth of impurities are effectively inhibited on the other hand, so that the fermentation process is simplified; the newly screened strains grow faster under the facultative oxygen condition, so that the viable bacterium concentration and the spore concentration of the clostridium butyricum in a co-fermentation system are higher, and a new energy-saving and environment-friendly mode is provided for production of the clostridium butyricum.

The invention relates to low-starch (starch level being less than 10 percent) expanded floating feed for carnivorous fishes. A part of protein raw materials of the feed uses novel microbial proteins,namely clostridium ethanol proteins, and the clostridium ethanol proteins are suitable for the carnivorous fishes which are intolerant to the starch. A contrast experiment proves that the feed disclosed by the invention can greatly reduce the consumption of fish meal, can meet requirements of nutrients required by growth and development of the carnivorous fishes, also can obviously reduce volume weight of the feed, and also can improve radial expansion rate, grain hardness, gelatinization degree and in-water stability of the low-starch feed; and moreover, through improving an inside hole diameter structure of granules, the oil absorption rate is improved, and the oil leakage rate is reduced. By adopting the low-starch expanded floating feed disclosed by the invention, the stable processingquality of the low-starch expanded floating feed is also obviously improved.

Clostridium perfringens can cause food poisoning and is a major agent in necrotic enteritis. As laws banning the use of antibiotics in animal feed become more common, the need for alternatives to antibiotics becomes greater. Peptidoglycan hydrolases that target the cell wall of specific bacteria are one such alternative. Genes for four endolysins, PlyCP10, PlyCP18, PlyCP33, and PlyCP41, were found within clusters of phage associated genes, likely prophages from strains Cp10, Cp18, Cp33, and Cp41. PlyCP18 and PlyCP33 harbor L-alanine amidase catalytic domains, and PlyCP10 and PlyCP41 have glycosyl hydrolase catalytic domains as predicted by BlastP and PFAM searches. All four genes were synthesized with E. coli codon optimization, expressed in E. coli expression vectors with a 6×His tag for nickel column purification, and the recombinant proteins purified. The four endolysins were capable of lysing the 66 C. perfringens strains tested but not the other bacteria tested.

The invention provides a microbe used for degrading deoxynivalenol, which belongs to microbe and feed additive fields. The microbe for degrading deoxynivalenol is obtained from the content in animal intestinal tract by pressure selection, gradient dilution and separation as well as screening, and is clostridium sp. by identification. The stability of the strain for degrading deoxynivalenol is good, in a L10 medium, the subcultring is carried out for 8-12 generations, the degradedation rate of DON is 100%. In the L10 medium, the initial concentration of DON is 25-100 ppm, the pH value is 5.0-8.0, anaerobic cultivation is carried out for 48-96 hours at the temperature of 30-40 DEG C, and the degradation rate of DON is more than 90%.

The invention discloses a tetanus toxin-resistant neutralizing antibody as well as preparation and application thereof. The antibody can be used for preventing, treating and diagnosing tetanus infection and / or treating one or more symptoms mediated by clostridium tetani infection. The invention further provides a method for producing an antibody capable of combining with tetanus toxin immunologically specifically.

The invention discloses a preparation method and application of a feed additive, and belongs to the field of feed additives. The preparation method of the feed additive comprises the following steps of S1, treating the main materials; S2, carrying out aerobic fermentation: (1) feeding the main material into a tank; (2) adding auxiliary materials; (3) inoculating bacteria A and bacteria B, whereinthe bacteria A are aspergillus oryzae spore powder, and the bacteria B are saccharomyces cerevisiae; (4) performing turning and throwing; (5) inoculating bacteria C and bacteria D, wherein the bacteria C are lactobacillus casei, and the bacteria D are clostridium butyricum; (6) inoculating bacteria D; S3, carrying out anaerobic fermentation; S4, taking out of a pool for modulation; and S5, performing packaging and delivery inspection. The feed additive prepared by the invention is obtained by asynchronous fermentation of multi-strain combination and control of certain fermentation conditions,not only has multiple functions of food calling, immunity improvement, feed conversion ratio reduction, egg laying rate improvement and the like, but also can be simultaneously applied to feeding of animals such as poultry, livestock, aquatic products and the like, and is a multifunctional, healthy and safe feed additive.

The invention relates to rumen bacteria and application thereof, and belongs to the technical field of microorganism. Aiming at the problem of low rate and low yield of hexanoic acid production of hexanoic acid production bacteria in the prior art, the invention provides a new strain of rumen bacteria (Clostridium sp.) CPB6 GDMCCNo:60133. The colonial morphology of the strain is a round and white bump, and thick in character. Scanning electron microscopy shows that the rumen bacteria is short-rod-shaped, the size of the rumen bacteria is 2 mu m * 0.5 mu m, the rumen bacteria is Gram staining positive bacteria, the rumen bacteria suitable growth temperature is 30-40 DEG C, pH is 5.0-6.5, and an acid and a gas can be produced by anaerobic growth for fermentation of glucose. The strain can use lactic acid for production of hexanoic acid, hexanoic acid production concentration is 16.6g / L, the highest yield can reach 5.29g / L / d; the strain also can be used for the production of the high added value of hexanoic acid from organic waste and wastewater containing the lactic acid, and waste resource recovery and recycling can be achieved.

The invention belongs to the technical field of wine brewing microorganisms, and particularly relates to a clostridium new strain and application thereof. Aiming at the problem that the variety of functional microorganisms capable of improving aroma in the white spirit fermentation process is less, the invention provides a clostridium sp. new strain with the preservation number of CGMCC NO.19455. The new strain disclosed by the invention can simultaneously produce butyric acid, ethyl valerate, ethyl acetate and 2-pentanone at high yield, provides a new strain for aroma production in white spirit brewing, enriches the resources of brewing microorganisms, and has a very good application value.

The invention discloses a culture method for improving the activity of clostridium thermoceuum cellulase, which comprises the following steps of (a) preparing a strain DSM1237T into a first-level strain liquid; (b) adding the first-level strain liquid into a base culture medium, wherein the volume ratio of the first-level strain liquid to the base culture medium is (1:10)-(1:20); performing fermentation culture for 48-72 hours to obtain a second-level strain liquid; (c) adding the cultured second-level strain liquid into the prepared fermentation culture medium, wherein the volume ratio of the second-level strain liquid to the fermentation culture medium is (1:30)-(1:10); (d) performing fermentation culture at a temperature of 50-70 DEG C under an anaerobic condition at a pressure of (-0.1)-0.1MPa, and standing, wherein the pH value is 6.5-8.0, and the culture time is 48-72 hours. According to the method disclosed by the invention, under the anaerobic condition, the energy consumption for introducing oxygen or compressed air in the fermentation process can be saved. Under the condition of same culture cost, the activity of the fermentation liquid cellulase is remarkably improved, and the economic benefits are remarkably increased.

The invention provides anaerobic cellulose decomposition bacteria (Clostridium sp.Lwy-2, wherein the preservation register number is CCTCC NO:M208161; the preservation date is on October 17th in 2008; the preservation unit is China Center for Type Culture Collection; and the address of China Center for Type Culture Collection is in the Wuhan University.). The bacteria have strong decomposition ability for the filter paper and also show stronger decomposition ability for the straw. And the invention also provides a culture medium for carrying out enrichment and separation culture on the anaerobic cellulose decomposition bacteria, and a method for carrying out co-culture on the cellulose decomposition bacteria and sulfate reducing bacteria (Desulfovibrio halophilus RETECH-SRB-I, wherein the preservation unit is China Center for Type Culture Collection; the address is in the Wuhan University; the preservation data is on May 11th in 2007; and the preservation register number is CCTCC NO:M207060.). The method can carry out co-culture on the cellulose decomposition bacteria and the sulfate reducing bacteria and utilizes degradation products of the cellulose decomposition bacteria system for the filter paper as a carbon source of the growth of the sulfate reducing bacteria.