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Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans

A technology of Bacillus coagulans and solid-state fermentation, applied in the field of microbial fermentation, to achieve the effects of simple fermentation management, low energy consumption, and simple operation

Active Publication Date: 2014-03-12
惠农达生物技术(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preparation of Clostridium butyricum complex probiotics by solid-state fermentation of mixed bacteria is rarely reported

Method used

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  • Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans
  • Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans
  • Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Clostridium butyricum activation and configuration of seed liquid medium (g / L): glucose 8, tryptone 10, yeast extract 4, K 2 HPO 4 5. MgSO 4 ·7H 2 O0.2, MnSO 4 ·H 2 O0.2, solvent is water, pH7.4. The activation medium is divided into test tubes, each with a capacity of 10 mL; the seed medium is divided into 50 mL flat-bottomed flasks, each with a capacity of 40 mL, and sterilized at 121°C for 20 minutes.

[0037] Put the strains frozen in the glycerol tube into the activated medium, and anaerobically culture at 37°C for 18 hours; the activated Clostridium butyricum TK2 was inoculated into the seed medium with an inoculum size of 5%, and cultured anaerobically at 37°C for 24 hours .

Embodiment 2

[0039] The configuration of Bacillus coagulans TQ33 seed medium (g / L): yeast extract 10, glucose 60, peptone 10, MgSO 4 ·7H 2 O0.5, the solvent is water, pH7.2, 50mL of seed culture medium is divided into each 250mL Erlenmeyer flask, and sterilized at 115°C for 20min.

[0040] Inoculate the Bacillus coagulans TQ33 preserved on the slant of the test tube into the seed medium, the inoculum amount is 1-3 rings, and culture at 37°C and 180r / min for 20h with shaking.

[0041] 2. Configuration of solid-state fermentation medium

Embodiment 3

[0043] Preparation of enzymatically hydrolyzed soybean meal: the weight ratio of soybean meal to water is 1:2, the amount of neutral protease is 1000u per gram of soybean meal, and the enzyme is hydrolyzed for 3 hours. The neutral protease ZDB-G-20 used was purchased from Tianjin Nuoao Technology Development Co., Ltd.

[0044] Configuration of solid-state fermentation medium: enzymolysis soybean meal 33.1, rice bran 6.6, MgSO 4 ·7H 2 O0.12, MnSO 4 ·7H 2 O0.12, add water to 100%, the volume of each 100mL triangular bottle is 70g, and the pH is natural. First dissolve the inorganic salt with water, then add enzymatically hydrolyzed soybean meal and rice bran in sequence while stirring, and finally make it evenly mixed, divide and sterilize, and sterilize at 121°C for 20 minutes.

[0045] 3. Effect of different inoculum amounts of Bacillus coagulans TQ33 on solid-state anaerobic fermentation of mixed bacteria

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Abstract

The invention relates to a solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans, wherein a production method is a solid state fermental cultivation method of the mixed bacteria. A solid state fermentation culture medium is formed with enzymolysis bean pulp and rice bran as major ingredients and added with proper amount of an inorganic salt; the solid state fermentation culture medium is subpackaged and then sterilized; next, the solid state fermentation culture medium is inoculated with an isometric clostridium butyricum TK2 seed solution accounting for 10% of the total weight of the solid state culture medium and an isometric bacillus coagulans TQ33 seed solution accounting for 5% of the total weight of the solid state culture medium, and stirred evenly for fermentation. After the fermentation is carried out at 37 DEG C for 48 hours, a compound probiotics preparation of the clostridium butyricum TK2 and the bacillus coagulans TQ33 is obtained by drying at 50 DEG C, crushing and sieving; in the fermentation of the mixed bacteria, antinutritional factors in the bean pulp are decomposed more effectively under the mutualistic synergistic effect of the clostridium butyricum and the bacillus coagulans; as a result, nutrient substances in the fermentation bean pulp are richer and more balanced; therefore, a high protein feed rich in compound probiotics is provided.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a solid-state fermentation method for mixed bacteria of Clostridium butyricum and Bacillus coagulans. Background technique [0002] Clostridium butyricum is an anaerobic, Gram-positive bacillus that is a normal flora of the intestinal tract of humans and animals and is also present in soil, cheese, and natural yoghurt; flagellated, motile; spores are eccentric or secondary Terminal (endophytic), round or oval, without spores and appendages. Clostridium butyricum has the physical and chemical properties of resisting relatively harsh external environments. It can withstand high temperature, storage resistance and good stability. In vivo, Clostridium butyricum can tolerate the effects of gastric juice, bile acid and digestive juice; It is sensitive to a few antibiotics, such as vancomycin, vancomycin and tetracycline, but is highly resistant to other antibiotics, and has a good ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/00C12R1/145C12R1/07
Inventor 王海宽路福平张善亭
Owner 惠农达生物技术(江苏)有限公司
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