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Use of clostridium butyricum in preparing medicine composition for preventing and curing intestinal cancer

A technology of Clostridium butyricum and a composition is applied in the application field of Clostridium butyricum in the preparation of pharmaceutical compositions for preventing and treating intestinal cancer, can solve the problems of unsatisfactory effect, large side effects, patients dying from side effects and the like, and achieves elimination of production and production. Toxin source, prevention of infection, and the effect of improving immunity

Inactive Publication Date: 2008-11-19
QINGDAO EASTSEA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although radiotherapy, chemotherapy, and immunotherapy have obvious effects, the side effects are very large, and patients are difficult to tolerate, and some patients even die of side effects
Although the side effects of traditional Chinese medicine are small, the effect is not ideal

Method used

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  • Use of clostridium butyricum in preparing medicine composition for preventing and curing intestinal cancer

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0035] Medicine preparation embodiment 1, Clostridium butyricum viable bacteria powder preparation:

[0036]Take a live Clostridium butyricum strain tube, dissolve it in a sterilized 100ml Erlenmeyer flask filled with 10ml normal saline and glass beads, activate for 10 minutes, use a 1ml sterile pipette to draw 1ml of the bacterial suspension to inoculate a 50ml expansion flask Place in a 250ml Erlenmeyer flask of growth medium, place in a rocking bed at 37°C for 24 hours at a constant temperature and oscillate (190rpm), transfer to a 2500ml baffle Erlenmeyer flask with 450ml of expansion medium, and culture for 24 hours at 37°C for 24 hours with constant temperature oscillation, microscopic examination Transfer to a seed tank with 4.5L of expansion medium after no bacteria, anaerobic culture (aeration volume 3:1) for 24 hours, and transfer to a fermenter with 45L of fermentation medium after microscopic examination without bacteria In the medium, 37 ℃ anaerobic culture for 24...

preparation Embodiment 2

[0037] Medicine Preparation Example 2, Clostridium butyricum Live Bacteria Capsule Preparation

[0038] Composition % (weight)

[0039] Clostridium butyricum live bacteria powder 20.00 parts

[0040] Microcrystalline cellulose 40.00 parts

[0041] Glucose 40.00 parts

[0042] Fully mix the above-mentioned components in groups 1 to 3, and then use conventional filling capsule technology to make capsules according to unit doses.

experiment example 1

[0043] Curative Effect Observation Experimental Example 1, Experimental Study on the Prevention of Colon Cancer by Clostridium Butyricum

[0044] 1. Experimental method

[0045] Select 40 female Kunming mice with a body weight of 18-20 g, and randomly divide them into a treatment group and a control group (20 mice in each group), and the experiment time is 20 weeks.

[0046] The carcinogen is 1,2-dimethylhydrazine (symmetrical 1,1-dimethylhydrazine, DMH), which is a white powder crystal, and it is made into a 0.4% solution with sterile physiological saline before each injection, and mixed with NaHCO 3 Its pH was adjusted to 6.5. The mice were injected intraperitoneally with DMH 20 mg / kg (ie 0.05 ml / 10 g of 0.4% DMH solution) once a week for 20 consecutive weeks. One week after the intraperitoneal injection of DMH, dextran sodium (DSS) was added to the drinking water at a concentration of 20 g / L, and the water was drunk continuously for 7 days.

[0047] Administration group,...

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Abstract

The invention discloses the novel application of clostridium butyricum to drugs, in particular to the application of the clostridium butyricum to the preparation of a drug combination used for preventing and treating intestinal cancer. The novel application comprises the prevention of chronic intestinal disease canceration such as intestinal polyp, Crohn disease, ulcerative colitis, etc., the adjuvant therapy on advanced inoperable intestinal cancer, the adjuvant therapy after the intestinal cancer operation, etc.; the clostridium butyricum is selected from (but is not limited to) clostridium butyricum, CGMCC, and storage serial number 0313.1. In the forms of live bacteria and pharmic receivable different carriers, the clostridium butyricum is made into tablets, capsules, powders, liquid preparations, suppositories, and enemas; the clostridium butyricum can be used for preventing and treating the intestinal cancer for a long time through the methods of oral administration, clysis, injection in the tumor tissue; therefore, the life of a patient suffering from the intestinal cancer is prolonged, and the life quality of the patient suffering from the intestinal cancer is increased.

Description

technical field [0001] The present invention relates to a new drug application of Clostridium butyricum, in particular to the application of Clostridium butyricum in the preparation of a pharmaceutical composition for the prevention and treatment of intestinal cancer, wherein the Clostridium butyricum is used as the main active ingredient of the medicine to make live bacterial preparations, which belongs to the biological technology field. Background technique [0002] Recent studies have shown that, contrary to the decline in the incidence of most cancers year by year, the incidence of bowel cancer in the world is increasing at an average annual rate of 2%, and a survey in Shanghai shows that the growth rate of the incidence of bowel cancer in my country is the world average. doubled to an annual average of 4%. Colorectal cancer is already one of the leading cancer killers, with 140,000 people diagnosed each year. There are also surveys showing that the age of onset of col...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P35/00A23L1/30A23L33/135A61K35/742
CPCY02A50/30
Inventor 崔云龙李洪福
Owner QINGDAO EASTSEA PHARMA
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