86results about How to "Promote cell adhesion" patented technology

The present invention relates to a biocompatible, three-dimensional scaffold useful to grow cells and to regenerate or repair tissue in predetermined orientations. The scaffold is particularly useful for regeneration and repair of cardiac tissue. The scaffold contains layers of alternating A-strips and S-strips, wherein the A-strips within each layer are aligned parallel to each other and preferentially promote cellular attachment over attachment to the S-strips. Methods of producing and implanting the scaffold are also provided.

The present invention claims and discloses a novel apparatus and method for efficiently cultivating cells with minimal mortality in order to harvest a maximum amount of cellular products generated by the cultivated cells. More particularly, the present invention teaches a method and a device for plating cells and causing maximum adherence of cells of interest. Furthermore, the present invention also teaches a growth substrate means that is capable of providing the largest surface area for cell adhesion and functions as an oxygenator, a depth filter and a static mixer to maximize the production of cellular products by intermittently and periodically provide sufficient oxygen and nutrients to the cells without causing cell death. The device of the present invention is economical and can be disposable thus eliminating complications caused by sterlization and is capable of periodically and intermittently provide oxygen and nutrients to cells, through controlling the amount of culture medium that comes into contact with the growth substrate means.

The present invention relates to a polymerization-curable composition for the preparation of biodegradable, biocompatible, cross-linked polymers on the basis of polyvinyl alcohol comprising: 5 to 100% by weight of (a) vinyl ester monomer(s) of one of the general formulas (I) to (III):

wherein X is oxygen, sulfur, nitrogen, or phosphorus; n is 1 to 1000, at least 20% of the n being ≧2; the R¹ are selected from hydrogen; straight, branched or cyclic, saturated or unsaturated, n-valent hydrocarbon groups having 1 to 30 carbon atoms, which optionally have heteroatoms and are optionally substituted with one or more substituents selected from —OH, —COON, —CN, —CHO, and ═O, and n-valent radicals of biodegradable, biocompatible oligomers and polymers; m is an integer from 1 to 5; the R² are selected from hydrogen, —OH, ═O, and the options listed for R¹; and the R³ are selected from hydrogen, —OH, and the options listed for R¹; 0 to 50% by weight of ethylenically unsaturated co-monomers; 0 to 10% by weight of (a) polymerization initiator(s); and 0 to 95% by weight of solvent(s).

The present invention provides a novel substrate for use in growing cells and for the study of mechanobiology. The membrane of the present invention comprises appropriate microtopography and surface chemical modifications to facilitate the production of adherent and oriented cells that phenotypically resemble cells in vivo.

The present invention provides bionanocomposite materials comprising at least two coaxial layers of bionanocomposites, and methods for producing and using the same. The bionanocomposite materials of the present invention comprise a core structure and a shell structure encapsulating the core structure, where one of the core structure or the shell structure comprises a porous biocompatible natural-derived material and the other comprises a biocompatible biomimetic nanostructure.

A high strength surgical material with improved tie down characteristics and tissue compliance formed of ultrahigh molecular weight polyethylene (UHMWPE) yarns, the suture being coated with arginine-glycine-aspartate (RGD) peptide. The suture has exceptional strength, is ideally suited for most orthopedic procedures, and can be attached to a suture anchor or a curved needle.

An object of the present invention is to provide a cell culture support making the detachment of a cell sheet easy as well as enabling the formation of a uniform cell sheet. The present invention relates to a method for manufacturing a cell culture support having a temperature responsive polymer immobilized onto the surface thereof via covalent bonding, the method including a coating step in which a composition including a monomer that can form the polymer by polymerization by radiation irradiation, an organic solvent and, in some cases, a prepolymer formed by polymerization of the monomer is coated onto the substrate having a surface containing a material which can be covalently bonded to the temperature responsive polymer by radiation irradiation to form a film on the surface of the substrate, a radiation irradiation step in which a polymerization reaction and a binding reaction between the substrate surface and the temperature responsive polymer are allowed to proceed by irradiating radiation to the film, and a drying step to dry the film.

Substrates for influencing the organization, spreading or adhesion of a selected cell to induce or stimulate growth, differentiation or regeneration of the cell or of tissue constituting the cells are provided as well as methods of making such substrates and methods of using such substrates.

The present invention relates to a polypeptide, a production method and uses thereof, wherein the polypeptide comprises an N-terminal region and a C-terminal region, and has remarkable cell adhesion effect.

A method for preparing a sericin hydrogel, the method including: 1) weighing a cocoon of a fibroin-deficient mutant silkworm, Bombyx mori, extracting the cocoon by an aqueous solution of LiBr or LiCl, dialyzing an extracted solution to yield a sericin solution having a concentration of a non-degraded sericin of between 0.1 and 4 wt. %; and 2) concentrating the sericin solution to a concentration of between 1.5 and 10 wt. %, adding a crosslinking agent to the concentrated sericin solution at a ratio of between 2 and 500 μL of the crosslinking agent per each milliliter of the sericin solution, fully blending the crosslinking agent with the concentrated sericin solution, and keeping a resulting mixture at the temperature of between 4 and 45° C. for between 5 s and 36 hrs to yield a hydrogel.

The invention provides a preparation method of a DA-based gradient functional material. DA serves as a functional group, and the chemical reactivity, oxidation auto-polymerization and adhesion of DA are utilized to prepare the functional material with a multistage pore channel and a gradient structure. The method includes the steps that firstly, Alg is subjected to graft modification with DA to prepare biologically-modified macromolecular Alg-DA with excellent adhesion performance; then, DA is subjected to oxidation auto-polymerization assembly in a slightly alkaline buffer solution to form PDA particles of a uniform particle size; then, Alg-DA of different concentrations and the PDA particles act on each other to form a first-stage cross-linked structure, and then the gradient functional material is prepared with a lamination freeze-drying method; then, the gradient functional material is cross-linked again with calcium ions, and the cross-linking degree and porosity of the material are further adjusted. The DA-based gradient functional material provides a new way for preparing composites with an ideal texture. The gradient functional material prepared through the method has high mechanical performance and excellent biocompatibility and biodegradability, can effectively improve the absorption separation performance and the regeneration performance of soft/hard tissue and has broad application prospects in the fields of multifunctional separation and absorption films and biomedicine.

The invention discloses an agricultural microbial agent microcapsule based on cell membrane mimicking and a preparation method thereof. The effect of regulating the osmotic pressure of cells is realized by regulating the moisture content of microbial cells and the flux of oxygen; and the wall material of the microcapsule is selected from gamma-polyglutamic acid (gamma-PGA), a polysaccharide material and dopamine. The gamma-PGA can achieve a synergistic effect with a microbial agent so as to enhance the antioxidant ability of plants, alleviate damage to plants under abiotic stress and promote plant growth, and is a typical microorganism-derived biostimulant; and a compact film formed by auto-polymerization of the dopamine on the surfaces of bacteria can effectively relieve the influence ofexternal osmotic pressure on the bacteria in the microcapsule, and polymerization reaction conditions are mild and have small damage to the bacteria. The preparation method disclosed by the inventionis based on a cell surface polymerization technology, and can realize single-cell coating of the microbial agent and inhibit proliferation of the microbial agent.

Substrates for cell culture and tissue engineering bioreactors consisting of polymers that change their shape over time under stimulation by temperature change, hydration, degradation, or other means. A method of controlling cell culture using a biodegradable shape memory polymer, wherein shape changes can transfer stresses, strains, or both to adherent or otherwise connected cells such that the mechanical stimulus impacts cell development and the resulting properties of tissues.

The invention provides EPS (Expandable Polystyrene) and mussel extract compounded skin barrier repairing dressing and a preparation method thereof. The EPS and mussel extract compounded skin barrier repairing dressing comprises the following raw materials in percentage by weight: 0.05%-0.12% of hyaluronic acid, 0.5%-0.8% of sodium alginate, 0.06%-0.15% of tremella heteropolysaccharide, 6%-8% of glycerol, 0.3%-1% of dihydric alcohol, 1%-2% of trehalose, 1%-2% of mannose, 0.02%-0.05% of lactic acid bacteria exopolysaccharide, 0.03%-0.08% of a mussel extract, 1%-2% of betaine, 1.5%-2.3% of amino acid, 1.8%-2.5% of PCA (pyrrolidone carboxylate) sodium, 0.01%-0.03% of a preservative, and the balance of water. According to the preparation method of the EPS and mussel extract compounded skin barrier repairing dressing, the lactic acid bacteria exopolysaccharide and the mussel extract are compounded, the tremella heteropolysaccharide, the trehalose, the mannose and the dihydric alcohol are combined, the betaine, the PCA sodium and the like are matched, and optimized proportion is adopted, so that the prepared dressing has good anti-inflammatory activity and high skin wound healing rate; and thus, the dressing can better and quickly repair damaged skin, has higher skin barrier repairing capacity, and is ensured with good tolerance.