364 results about "Proteinogenic amino acid" patented technology

The invention provides an mVSV virus vector, i.e., attenuated mVSV obtained after multiple modification mutations occur to an M protein amino acid site of a wild Indiana strain VSV, and an optimized heterologous antigen gene is preferentially integrated to a double cloning site area of an mVSV packaging core plasmid pmVSV-Core at the same time. The mVSV virus vector vaccine comprises a heterologous antigen gene which fuses or embeds a target virus between G and L genes of an mVSV vector envelope, wherein the antigen gene comprises an enveloped and embedded antigen gene encoding the target virus, an embedded combination antigen gene or a fused antigen gene; the mVSV virus vector is embedded or fused with a dominant antigen of spike protein S of an SARS-CoV-2 pathogen; the dominant antigen is preferably selected from a receptor binding domain of spike protein S, namely RBD; and a COVID-19 vaccine based on mVSV mediation is formed. The vaccine has good prevention or treatment effect on COVID-19 infected people.

The invention discloses a bacterial laccase mutant protein, which is characterized in that the mutant protein amino acid sequence is obtained by deletion mutation of the 323rd glycine residue to the 332rd glycine residue in the bacterial laccase amino acid sequence shown as SEQ ID No.1. Through a genetic engineering reconstruction method, a stability improved bacterial laccase protein coding gene, its expression plasmid and engineered bacteria can be obtained, and after large-scale fermentation and induced expression of the engineered bacteria, the stability improved bacterial laccase protein can be obtained. According to the invention, the marine uncultured microorganism source bacterial laccase Lac15 is taken as the foundation, and by means of genetic engineering reconstruction, mutant gene can be obtained. At the same time, a recombinant escherichia coli is employed to conduct high-density culture for high-efficiency expression of the bacterial laccase mutant protein. According to the invention, the stability and yield of the bacterial laccase are greatly improved.

The invention relates to a hair-care composition containing an efficient anti-hair-loss compound agent. The hair-care composition comprises long-chain alcohol, a cationic surface active agent, a thickening agent, essence, a preservative, a chelating agent, water and an anti-hair-loss compound active matter, wherein the anti-hair-loss compound active matter accounts for 0.1 to 10 percent of weight of the composition and is a composition of hydrolyzed soy protein, amino acid, amino sugar, vitamin B complex, zinc salt, ginseng essence, May burdock root essence, organic silicon and polyquaternium. By virtue of the hair-care composition, the hair growth can be promoted, the ratio of a growing period to a resting period of hair is improved, forming of healthy scalp and healthy hair is promoted, and the hair loss speed of a hair loss patient is returned to be normal.

An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as the main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation of cells with a DNA coding for dihydrodipicolinate synthase that is desensitized to feedback inhibition by L-lysine and a DNA coding for aspartokinase that is desensitized to feedback inhibition by L-lysine, or a Methylophilus bacterium which is casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

The invention discloses a high-sugar and low-protein tilapia feed. Based on the total mass fraction of 100%, it consists of the following components: fish meal: 0-3%, soybean meal: 16-20%, rapeseed meal: 18-21%, cotton meal: 12-16%, secondary meal: 39- 42%, soybean oil: 2-3%, choline chloride: 0.2-0.5%, sodium chloride: 0.1-0.5%, calcium dihydrogen phosphate: 1-2%, vitamin B1: 0.004-0.006%, vitamin B6: 0.002-0.004%, zinc sulfate: 0.02-0.04%, chromium chloride: 0.0001-0.0003%, vitamin premix: 0.1-0.2% and mineral premix: 0.5-1%. The invention maximizes the amount of secondary powder in the feed, adds several plant protein sources to overcome the problem of amino acid imbalance of a single protein source, and at the same time significantly reduces the amount of fish meal. By optimizing and adjusting the feed formula, supplementing suitable vitamin and mineral premixes to adjust the sugar utilization ability of tilapia, so that the tilapia can make good use of the sugar compound of the present invention, avoiding the damage of fish liver caused by high sugar, Fatty liver is formed, and even the fish body becomes obese, and the symptoms of "big belly" appear.

The invention discloses a recombinant human collagen and an application thereof. An amino acid sequence of the protein is shown as SEQ ID NO. 3, the nucleotide sequence of the protein-coding gene is shown as SEQ ID NO. 1. The recombinant human collagen of the invention has very good hydrophilicity and stability, and the amino acid composition thereof is 100% identical to the corresponding amino acid sequence of the natural collagen, and the amount of expressed protein can account for about 25% of the total protein of the thalline, per milliliter of the bacteria liquid precipitate contains 0.25mg of the target protein, and the production cost is very low and the cycle is short. The prepared collagen is applied to the human body without immunological rejection and allergic reaction, and canbe widely applied to the biomedicine and cosmetics industries.

The invention discloses a rebuilt collagen and the compounding and expression purifying method. The main body of the collagen amino acid sequence is triad Gly-Xaa-Yaa, of which Xaa and Yaa at least have an electrified amino acid residue. The N end and C end contains the sequence of cysteine residue; the Arg-Gly-Asp residue combination is contained in the entire amino acid sequence. Using the compounded oligonucleotide to take annealing treatment to form double helical target DAN monomer that takes repeatedly polymerizing to form macromolecule DNA which would transfer into coliform bacteria, thus, the rebuilt collagen would be gained. The invention has good application value and could be used in biology medicine material, cosmetic and food fields.

The invention relates to 86 types of particular mutation type atlases of SLC26A4 gene related to hearing loss in Chinese crowd, 27 types of relative hot-spot mutation type atlases and frequency information thereof, 13 types of hotspot mutation type atlases and frequency information thereof, and 2 types of hottest-spot mutation type atlases and frequency information thereof. 59 SLC26A4 gene mutation types are newly discovered in the Chinese crowd, wherein, 47 mutation types lead to the change of encoded protein amino acid of the SLC26A4 gene or influence genetic transcription and translation, 6 mutation types lead to base change rather than the change of amino acid, and 6 types are intron mutation types of the SLC26A4 gene. The discovery has a vital practical significance in developing an SLC26A4 gene diagnosing chip and a kit, which conform to hereditary features of the Chinese crowd suffering hearing loss.

The present invention discloses a kind of new type of fabric containing protein fiber and essence microcapsule and with skin care function and its preparation process. The fabric consists of filament of protein fiber with skin care function and superfine fiber as base material and through blending and weaving, has adsorbed essence microcapsule, and treated through mosquito repelling treatment, grafting copolymerization treatment and coating treatment. The fabric can release 18 kinds of fibrion amino acids, and has the functions of strengthening skin metabolism, preventing blood vessel cirrhosis and delaying skin senility, as well as resisting oxidation, beautifying skin and maintaining water.

The invention discloses a recombinant I-type humanized collagen polypeptide as well as a preparation method and application thereof. The recombinant I-type humanized collagen polypeptide provided by the invention comprises n repeats of a sequence shown in SEQ ID No. 1, wherein n is an integer greater than or equal to 1, and when n is an integer greater than or equal to 2, the repeat sequences are directly connected; and optionally, the N tail end of the recombinant I-type humanized collagen polypeptide comprises an amino acid sequence which can be excised by TEV protease. The recombinant I-type humanized collagen polypeptide provided by the invention has the activity of promoting cell adhesion, the amino acid sequence of the recombinant protein is selected from a natural collagen amino acid sequence, and the recombinant protein does not generate an immune response when being applied to a human body; and moreover, the preparation method is simple, and high-yield collagen can be obtained at low cost.

The invention relates to mousse cream and a preparation method thereof. The mousse cream is prepared from the following components in parts by mass: polyglycerol-10 stearate, glycerol stearate citrate, polydimethylsiloxane, hydrogenated cocoglycerides, silicon wax, babassu seed oil, phytosterol oleate, tocopherol acetate, hydrogenated lecithin, acrylate alkyl acrylate crosspolymer sodium-2, PEG-240 / HDI copolymer bis-decyltetradeceth-20 ether, colloidal oat, decyl silicone oil, cyclomethicone / dimethiconol mixture, propylene glycol, resveratrol, corn gluten amino acids, centella asiatica polypeptide, bifida ferment lysate, ceramide liposome, vaccinium myrtillus leaf extract, antiallergic active peptides and preservatives. The mousse cream can effectively solve the problems of skin aging, particularly fine lines, wrinkles, loose skin, rough skin, dry skin and dark skin.

The invention discloses a method for predicting flu antigen through a model and an application thereof. The method comprises the following steps: extracting 12 features affecting the flu antigen, wherein the 12 features include mutation numbers of five antigenic determinant amino acid, five physical and chemical properties of HA protein amino acid, factor affecting receptor, and number of glycosylated site, the five physical and chemical properties of amino acid include hydrophobicity, volume change, electriferous property, polarity and integrable surface area; and making statistics on the 12 features of 3681 virus pairs similar to known antigen and 1720 virus pairs variant from antigen, thereby establishing a prediction model on antigen relation. On the basis of sequence, the method can present the antigen relation among viruses, and thus having the advantages of simpleness, convenience and high sensitivity. The process of antigen evolution can be visually displayed through the network. The invention is used for revealing flu spreading rules, screening vaccine candidate strains and the like.

A protein powder features its ideal protein-amino acid structure mode and contains soybean protein, whey protein, stabilizer, lecithin and 9 amino acids necessary to human body. Its preparing process features use of boiling bed to spray lecithin and granulating.

The invention discloses a low-protein daily ration of a three-way cross growing pig and belongs to the technical field of a pig industry of animal husbandry. The formula of the daily ration comprises 66-68 parts of corns, 4-4..6 parts of wheat bran, 15.4-24 parts of soybean meal, 0.88-0.92 part of stone powder, 0.89-1.07 parts of calcium hydrophosphate, 0.4 part of edible salt, 0.168-0.44 part of L-lysine hydrochloride. 0.040-0.081 part of DL-methionine, 0-0.12 part of L-threonine, 0-0.03 part of L-tryptophan, 0.208-0.582 part of zeolite powder and 1.00 part of premixed powder. According to the technology disclosed by the invention, according to an ideal protein amino acid model of the three-way cross growing pig, the rough protein level of the daily ration of the three-way cross growing pig are reduced by three percent, so that the aims of keeping the growing performance, reducing the cost of a feed formula and reducing nitrogen content in excrements are achieved.

The invention discloses a cDNA sequence represented by the SEQ ID No.1 of a paddy rice leaf color control gene OscpSRP54 coding area and an amino acid sequence represented by SEQ ID No.2 of a protein encoded by the gene. The gene OscpSRP54 which controls the paddy rice leaf color is cloned from a paddy rice light green leaf mutant HM14 through a map-based cloning technology, and the gene OscpSRP54 is proved to be the gene that control the paddy rice leaf color through a functional complementation experiment. Through observation of chloroplast by using a transmission electron microscope, the fact that the OscpSRP54 gene has the function of modulating chloroplast growth and then further controlling the leaf color is proved. The OscpSRP54 gene can be used to modulate paddy rice chloroplast growth so as to improve the photosynthesis efficiency and increase the paddy rice output. The leaf color control gene provided by the invention can also be used as a tracing marker in a genetic transferred plant progeny or an indicating marker for distinguishing pure species from hybrid species in a hybrid seed production process, and can be applied to the fields of good species breeding and hybrid breeding.

The invention discloses a paddy rice herbicide resistant protein and gene as well as application of the protein and the gene. The herbicide resistant protein is obtained by mutating Ala179 and / or Gly628 in a paddy rice acetolactate synthase protein amino acid sequence shown in SEQ ID NO.1, or is obtained by mutating Ser627 and Va1643 in the paddy rice acetolactate synthase protein amino acid sequence shown in SEQ ID NO.1; the gene for encoding the herbicide resistant protein and the application of the herbicide resistant protein and / or the gene for encoding the herbicide resistant protein in preparation of herbicide resistant plants are provided. According to the herbicide resistant protein, the gene and the application disclosed by the invention, acetolactate synthase inhibiting herbicides can be used in a process of planting gramineous plant crops, so that the requirements on direct seeding, mixed seeding, transplantation, mechanical production and the like of gramineous plants can be met, and a good application prospect is achieved.

An environment-friendly textile sizing agent is composed of the following raw materials in parts by weight: 3 to 4 parts of crease resistant agent, 8 to 15 parts of acrylate, 5 to 7 parts of maleic anhydride, 10 to 16 parts of butanediol diacrylate, 2 to 4 parts of hydroxyethyl cellulose, 8 to 14 parts of glycol, 7 to 15 parts of butyl acrylate, 6 to 10 parts of aqueous polymerized ether-ester, 2 to 4 parts of protein amino acid, 2 to 4 parts of polyacrylate, 12 to 26 parts of acetylated distarch phosphate, 2 to 4 parts of wax sheet, 10 to 15 parts of protein slurry, 3 to 5 parts of modified silicone oil antifoaming agent, 2 to 4 parts of sodium sulfamate, 7 to 12 parts of cassava starch, and 9 to 13 parts of modified starch. The provided environment-friendly textile sizing agent has a good film-forming property and permeability, can reduce the pollution, has a simple preparation technology, and is accord with the environment protection requirements.

The invention pertains to the field of the biotechnology. The invention relates to a recombinant protein. The recombinant protein includes two dominant antigenic epitopes of humanized heart type fatty acid binding protein, the amino acid sequence of the recombinant protein is converted into corresponding nucleotide sequence by adopting codon preferred by escherichia coli, chemical synthesis is performed on the nucleotide sequence and recombinant expression vector is constructed, so that protein expression level of the recombinant protein in the escherichia coli is increased. The invention also relates to the preparation of monoclonal antibody of the recombinant protein. Immunization, cell fusion and several rounds of screening are carried out to obtain hybridoma cell strain, the monoclonal antibody is purified and labeled with horse radish peroxidase (HRP), the best matching combination of the monoclonal antibody is determined by ELISA orthogonal experiment, and an early diagnosis application of myocardial infarction is achieved.

The invention relates to a textile size applicable to polyester fabrics. The textile size comprises the following components in mass ratio: 50 parts of nano silicon dioxide modified starch, 26 parts of protein size, 20 parts of water-based copolyether ester, 2 parts of glycerol, 2 parts of modified silicone oil defoaming agent, 3 parts of argine monohydrochloride, 1 part of sodium sulfamate and 1 part of polyacrylate. The size has favorable film forming property and permeability, is particularly applicable to the sizing of the polyester fabrics and is more environment-friendly as the formula does not contain polyvinyl alcohol.

The invention discloses a mild hair-fixing anti-alopecia hair-growing essence and a preparation method thereof. The mild hair-fixing anti-alopecia hair-growing essence comprises the following raw materials: water, ethanol, fleeceflower root extract, glycerol, angelica sinensis extract, aloe extract, ginseng extract, tea polyphenol, corn gluten amino acids, vitamin H, folic acid, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, vitamin B1, yeast polypeptides, cysteine, sodium benzoate, potassium sorbate, citric acid, linoleic acid, xylanase and hydroid extract. The synergistic effect of the linoleic acid, the xylanase and the hydroid extract can improve the alopecia prevention rate and hair growth promoting rate of the mild hair-fixing anti-alopecia hair-growing essence.

The invention relates to a preparation method of air dried venison, belonging to the food processing field. The invention cuts selected and finished venison into 1.5-2 cm of blank and carries out natural air drying on the blank; seasoner is added to be pickled for 35 hours; the pickled venison slices are put into a stainless steel jacketed kettle; 10-20L of water, seasoner and spice are added into the electric heating jacketed kettle to be evenly mixed and boiled, and the pickled venison slices are added; the pickled venison slices are kept in the slight boiling state to be boiled for 1.5-2 hours, and the temperature of 95-100 DEG C is kept; the obtained product is cooled, dried and precooled; and the dried venison is packaged and sterilized. The invention does not contain harmful additive and artificial color, has no pollution and contains animal and plant high-protein amino acid and necessary cellulose for human body. The product has abundant nutrition, pure taste, is pleasant in after-taste and provides a convenient purely natural green food with abundant and multiple nutrition components for people. The invention has simple operation step, easy master and low manufacturing cost.

The invention discloses a textile sizing agent. The textile sizing agent is prepared from the following raw materials in parts by weight: 12 to 26 parts of nano silicon dioxide modified starch, 5 to 7 parts of kaolin, 3 to 4 parts of organic silicon defoamer, 2 to 6 parts of dacron, 4 to 8 parts of soybean protein fiber, 9 to 14 parts of acetate fiber, 3 to 6 parts of polyvinyl alcohol, 4 to 7 parts of protein amino acid, 2 to 5 parts of polyacrylic ester, 2 to 4 parts of hydroxymethyl cellulose, 4 to 7 parts of pentaerythritol stearate, 1.2 to 2.3 parts of essence, 6 to 12 parts of polyvinyl alcohol and 7 to 10 parts of acetic acid. The textile sizing agent has the beneficial effects of stable viscosity, complete gelatinization, prevention of environmental problem and simple production process.

The invention relates to textile a sizing agent and a preparation method thereof. The textile sizing agent comprises, by mass percentage, 82-88% of main materials and 12-18% of auxiliaries. The main materials comprise nano silicon dioxide modified starch, pea starch, phosphate starch, acrylic acid, butyl acrylate, acrylamide, sodium sulfamate, diphenylmethane and protein amino acid. The auxiliaries comprise methyl alcohol, 2-naphthol, monochloroacetic acid and NaOH. The preparation method of the textile sizing includes the steps that firstly, the main materials are prepared; secondly, the main materials and the auxiliaries are evenly mixed to obtain the textile sizing agent. When the textile sizing agent is used for sizing, textiles can be subjected to sizing in the temperature range of 25-28 DEG C; after sizing, yarn is stable in performance, and strength and wear resistance are enhanced.

The invention relates to a low-pollution textile sizing agent. The low-pollution textile sizing agent comprises following components in parts by mass: 64 parts of nano silicon dioxide modified starch, 18 parts of a protein sizing agent, 5 parts of polyvinyl alcohol, 11 parts of polyacrylate emulsion, 2 parts of a modified silicone oil de-foaming agent, 3 parts of protein amino acid, 4 parts of kaolin, 3 parts of sodium sulfamate and 1 part of polyacrylate. The sizing agent with the formula has good film-forming property and permeability; the formula contains less polyvinyl alcohol so that the pollution to the environment is low.

The present invention provides a nucleotide sequence encoding a protein having isatis indigotica fortune lariciresinol reductase activity, wherein the nucleotide sequence is characterized in that the nucleotide sequence is represented by SEQ ID NO.1. The present invention further provides an amino acid sequence of the protein having the isatis indigotica fortune lariciresinol reductase activity, wherein the amino acid sequence is characterized in that the amino acid sequence is represented by SEQ ID NO.2. The present invention further provides applications of the nucleotide sequence encoding the protein having the isatis indigotica fortune lariciresinol reductase activity in enhancement of the isatis indigotica fortune lariciresinol reductase expression of the plant. According to the present invention, the high expression of the isatis indigotica fortune lariciresinol reductase in the plant is easily achieved so as to increase the lariciresinol yield.

The invention relates to a textile size applicable to linen fabrics. The textile size comprises the following components in mass ratio: 75 parts of parts of protein size, 15 parts of nano silicon dioxide modified starch, 8 parts of glycerol, 2 parts of modified silicone oil defoaming agent, 6 parts of argine monohydrochloride, 3 part of sodium sulfamate and 1 part of polyacrylate. According to the textile size applicable to linen fabrics, the protein size is taken as a primary raw material, the nano silicon dioxide modified starch and glycerol are taken as secondary raw materials, the modified silicone oil is taken as a defoaming agent, and the four components are proportioned, so that the size has favorable film forming property and permeability, is particularly applicable to the sizing of the linen fabrics and is more environment-friendly as the formula does not contain polyvinyl alcohol.

The invention discloses a protein, the name of which is GhWRKY40, which is a protein shown in (a) or (b) or (c): (a) a protein with the amino acid sequence shown in sequence 2 in a sequence table; (b) a fusion protein obtained by connecting tags at the N end or / and the C end of the protein shown in the sequence 2 in the sequence table; and a protein with the function or activity of GhWRKY40 transcription factors which is obtained by deriving the protein shown in (a) or (b) through substitution, deletion or / and insertion of one or more amino acids. The gene has the advantages that the GhWRKY40 regulates transcription expression of specific genes, causes physiological and biochemical changes of plants, increases resistance of plants to pathogen invasion, so as to protect growth and development of plants, and has important significance in application of improving response of plants to pathogens.

The invention discloses an enteric cytopathogenic human orphan virus 6-type VP1 protein specific epitope, a fusion protein of the enteric cytopathogenic human orphan virus 6-type VP1 protein specific epitope and a preparation method and use of the fusion protein and relates to the field of a gene engineering technology, a vaccine and a diagnostic reagent. The preparation method comprises analyzing an enteric cytopathogenic human orphan virus 6-type surface protein VP1 amino acid sequence by a computer, and screening strong specific epitope-containing protein fragments comprising 75th-101th amino acids, 127th-141th amino acids, 204th-237th amino acids and 257th-289th amino acids, wherein the four protein fragments are connected by 2 glycines and 1 serine so that the epitope fusion protein is formed. A codon in favor with eucaryotes and prokaryotes is used, a brand new gene sequence of the epitope fusion protein is chemically synthesized, through a gene engineering technology, the enteric cytopathogenic human orphan virus 6-type VP1 protein epitope fusion protein is expressed and prepared and is used for development of an enteric cytopathogenic human orphan virus antibody detection reagent and preparation of an enteric cytopathogenic human orphan virus monoclonal antibody and polyclonal antibody.