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Agricultural microbial agent microcapsule based on cell membrane mimicking and preparation method thereof

A technology of agricultural microorganisms and cell membranes, applied in the field of microorganisms, can solve problems such as unfavorable industrialization and application of microcapsule products, poor uniformity of microcapsule size, and destruction of microbial agents, so as to enhance the antioxidant capacity of plants, alleviate damage, and prolong the lifespan of plants. The effect of validity

Active Publication Date: 2020-06-09
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The size uniformity of the microcapsules prepared by the emulsification method is poor, and vigorous mechanical stirring is required during the preparation process, which will greatly damage the activity of bacteria
The spray drying method requires short-term high-temperature drying, which will inevitably cause great damage to the bacteria, and has relatively high requirements for equipment.
To sum up, the existing microcapsule coating technology or its implementation process will have a great destructive effect on microbial agents, which is not conducive to the industrialization and application of microcapsule products.

Method used

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  • Agricultural microbial agent microcapsule based on cell membrane mimicking and preparation method thereof
  • Agricultural microbial agent microcapsule based on cell membrane mimicking and preparation method thereof
  • Agricultural microbial agent microcapsule based on cell membrane mimicking and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A, the preparation of the core material (microbial cells) and the wall material mixture, that is, the continuous phase: Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO.M209107, sterilized γ-polyglutamic acid, xanthan gum and Tris / HCl buffer solution (pH 7.4), mix thoroughly; the total number of bacterial colonies of Pseudomonas stutzeri in Tris / HCl buffer solution is 10 10 cfu / mL, the mass volume ratio of γ-polyglutamic acid and Tris / HCl buffer solution is 0.5g / 100mL; the mass volume ratio of xanthan gum and Tris / HCl buffer solution is 0.1g / 100mL;

[0041] B. Add a certain amount of dopamine into the above mixed solution (the mass volume ratio of dopamine to Tris / HCl buffer solution is 0.02g / 100mL), stir evenly, adjust the pH of the mixed solution to 8.5, and stir at 500rpm for 12h at room temperature. Dopamine is polymerized under alkaline conditions to obtain microspheres, which are dispersed in the continuous phase solution; the collected microspheres are washe...

Embodiment 2

[0045] A, the preparation of the core material (microbial cells) and wall material mixture, i.e. the continuous phase: Streptomyces lydicus (Streptomyces lydicus) CGMCC No.16840, sterilized γ-polyglutamic acid, seaweed polysaccharide and Tris / HCl buffer solution (pH 7.4), fully mixed; the total number of bacterial colonies of Streptomyces lydidi in Tris / HCl buffer solution is 9 × 10 9 cfu / mL, the mass volume ratio of γ-polyglutamic acid to Tris / HCl buffer solution is 1.0g / 100mL; the mass volume ratio of seaweed polysaccharide to Tris / HCl buffer solution is 0.5g / 100mL;

[0046] B. Add a certain amount of dopamine into the mixed solution (the mass volume ratio of dopamine to Tris / HCl buffer solution is 0.04g / 100mL), stir evenly, adjust the pH of the mixed solution to 8.5, and stir at 500rpm for 12h at room temperature. Dopamine is polymerized under alkaline conditions to obtain microspheres, which are dispersed in the continuous phase solution; the collected microspheres are was...

Embodiment 3

[0049] A, the preparation of the core material (microbial cell) and wall material mixture, that is, the continuous phase: Penicillium aspenicillium (P.asturianum) CGMCC No.17198, sterilized γ-polyglutamic acid, chitosan and Tris / HCl Buffer solution (pH 7.4), mix thoroughly; the total number of bacterial colonies of Penicillium aspenicillium in Tris / HCl buffer solution is 8×10 9 cfu / mL, the mass volume ratio of γ-polyglutamic acid and Tris / HCl buffer solution is 1.5g / 100mL; the mass volume ratio of chitosan and Tris / HCl buffer solution is 1g / 100mL;

[0050] B. Add a certain amount of dopamine into the mixed solution (the mass volume ratio of dopamine to Tris / HCl buffer solution is 0.05g / 100mL), stir evenly, adjust the pH of the mixed solution to 8.5, and stir at 500rpm for 12h at room temperature. Dopamine is polymerized under alkaline conditions to obtain microspheres, which are dispersed in the continuous phase solution; the collected microspheres are washed 5 times with Tris...

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Abstract

The invention discloses an agricultural microbial agent microcapsule based on cell membrane mimicking and a preparation method thereof. The effect of regulating the osmotic pressure of cells is realized by regulating the moisture content of microbial cells and the flux of oxygen; and the wall material of the microcapsule is selected from gamma-polyglutamic acid (gamma-PGA), a polysaccharide material and dopamine. The gamma-PGA can achieve a synergistic effect with a microbial agent so as to enhance the antioxidant ability of plants, alleviate damage to plants under abiotic stress and promote plant growth, and is a typical microorganism-derived biostimulant; and a compact film formed by auto-polymerization of the dopamine on the surfaces of bacteria can effectively relieve the influence ofexternal osmotic pressure on the bacteria in the microcapsule, and polymerization reaction conditions are mild and have small damage to the bacteria. The preparation method disclosed by the inventionis based on a cell surface polymerization technology, and can realize single-cell coating of the microbial agent and inhibit proliferation of the microbial agent.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a cell membrane biomimetic agricultural microbial agent microcapsule and a preparation method thereof. Background technique [0002] Soil beneficial microorganisms (agricultural microbial agents) can stimulate soil vitality through their own proliferation and secretion of active substances, increase soil aggregate structure, loosen soil, alleviate soil pollution and hardening caused by excessive chemical fertilizers, and can also be strongly decomposed and released Trace elements such as phosphorus, potassium, iron, and silicon deposited over the years increase soil nutrition. After entering the soil, the beneficial microorganisms in the soil form a mutual symbiotic multiplication relationship with the microorganisms in the soil, inhibit the growth of harmful bacteria and transform them into beneficial bacteria, interact and promote each other, and play a synergistic role ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N63/22A01N63/28A01N63/20A01N63/36A01N63/38C05F11/08C05G3/00C05G3/80A01P21/00C09K17/32C09K101/00
CPCA01N25/28C05F11/08C05G3/00C09K17/32C09K2101/00
Inventor 王瑞徐虹杨凯雷鹏詹伊婧冯小海李莎许宗奇高南
Owner NANJING UNIV OF TECH
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