The invention relates to a fruit and vegetable beef sauce and a preparation method thereof. The fruit and vegetable beef sauce is characterized in that the fruit and vegetable beef sauce is prepared from the following raw materials by weight: 80-85 parts of beef, 1.2-1.5 parts of pericarpium citri reticulatae, 1-2 parts of spinacia oleracea l, 0.8-1 part of fructus broussonetiae, 1-1.4 parts of loquat flower, 2-2.3 parts of apple flower, 0.8-1 part of sophora davidii (franch.) skeels, 1-1.2 parts of root of clammy hopseedbush, 4-5 parts of green vegetable, 3-4 parts of spinach, 5-6 parts of carambola, 4-5 parts of sparerib, 2-3 parts of condensed milk, 35-37 parts of chicken skin, 45-50 parts of wheat germ, 9-10 parts of red pepper, 7-8 parts of peanut oil, 400-450 parts of soybean sauce, and 7-8 parts of a nutritional additive. According to the present invention, the fruit and vegetable beef sauce has characteristics of delicate taste, spicy and delicious taste, and reasonable and scientific formula, the vitamin content is increased with the added vegetables, the added carambola and other fruits and vegetables, the wheat germ is rich in proteins, minerals, dietary fibers and the like so as to provide effects of intestine smoothing, constipation relieving, carcinogen discharge accelerating and intestinal cancer prevention, and the fruit and vegetable beef sauce contains a variety of Chinese herbal components so as to provide effects of qi regulating, ingredients, spleen strengthening, liver fire clearing and eyesight improving.