Culture medium and culture method for colorectal cancer organoids
A culture medium and organoid technology, applied in the field of biomedicine, can solve the problems of less research and reports on colon cancer organoid culture methods, test procedures, operation steps, culture conditions, and medium formulations, etc. Achieve the effect of reducing the risk of microbial contamination, reducing cytotoxicity and inhibitors, and improving success and survival rates
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Embodiment 1
[0038] This embodiment provides a culture medium for intestinal cancer organoids, including basal medium Advanced DMEM / F12, specific additive factors and sterile water; wherein, the basal medium Advanced DMEM / F12 and the sterile water The mass ratio is: 99:1; the specific addition factors include: vitamin A-free B27, 1×; N-acetylcysteine, 1.5 μM; EGF, 50ng / ml; Noggin, 200ng / ml; R-spondin 1, 200ng / ml; Wnt3a, 100ng / ml; CHIR99021, 5μM; thiazovivin, 2μM; Gastrin I, 15ng / ml; penicillin and streptomycin mixture, 1.2×; The concentration is based on the concentration in the mixture of basal medium and sterile water.
Embodiment 2
[0040]This embodiment provides a culture medium for intestinal cancer organoids, including basal medium Advanced DMEM / F12, specific additive factors and sterile water; wherein, the basal medium Advanced DMEM / F12 and the sterile water The mass ratio is: 99:1; the specific addition factors include: B27 without vitamin A, 2×; N-acetylcysteine, 1 μM; EGF, 80ng / ml; Noggin, 100ng / ml; R-spondin 1, 400ng / ml; Wnt3a, 80ng / ml; CHIR99021, 2μM; thiazovivin, 4μM; Gastrin I, 10ng / ml; penicillin-streptomycin mixed solution, 1×; The concentration is based on the concentration in the mixture of basal medium and sterile water.
Embodiment 3
[0042] This embodiment provides a culture medium for intestinal cancer organoids, including basal medium Advanced DMEM / F12, specific additive factors and sterile water; wherein, the basal medium Advanced DMEM / F12 and the sterile water The mass ratio is: 99:1; the specific addition factors include: B27 without vitamin A, 2×; N-acetylcysteine, 2μM; EGF, 20ng / ml; Noggin, 400ng / ml; R-spondin 1, 600ng / ml; Wnt3a, 150ng / ml; CHIR99021, 5μM; thiazovivin, 2μM; Gastrin I, 20ng / ml; penicillin-streptomycin mixture, 1.2×; All are subject to its concentration in the mixture of basal medium and sterile water.
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