134 results about "Steroidal alkaloid" patented technology

The present invention relates to steroidal alkaloids useful in the treatment of hedgehog pathway related disorders, particularly cancer.

The present invention relates to the field of plant secondary metabolites. More particularly plant cell lines are established from the medicinal plant Veratrum californicum. Said plant cell lines can be used for the production of cyclopamine and related steroid alkaloids and their precursors.

An obesity control agent with health-restorative and health-promotional benefits to humans comprising the extract of Chlorophytum species, more particularly, Chlorophytum arundinacceum, is disclosed. The bioactive principles responsible for anti-obesity property have been determined to be mainly due to spirosta-steroidal saponins, spirosta-steroidal alkaloids and galacto-glucan oligosaccharides. Most effective as an obesity control agent is the spirosta-steroidal saponins. Pharmaceutical, nutritional and veterinary use of this inventive composition is also disclosed.

The present invention relates to steroidal alkaloids useful in the treatment of hedgehog pathway related disorders, particularly cancer.

The invention discloses a method for simultaneously preparing total flavone extract and total alkaloid extract of Radix sophorae flavescentis. Total flavone extract mainly comprises flavanones (flavanonols) such as norkurarinone, kurarinone, and isokurarinon and other flaconoids such as trifolirhizin, xanthohumol, and kuraridin. Total alkaloid extract mainly comprises matrine n-oxide, N-oxysophocarpine, matrine, sophocarpine, sophoridine, and sophoramine. Total flavone extract and total alkaloid extract can be extracted through one or more of methods of solvent-extraction, solvent extraction, macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Flavonoid content in total flavone extract is 5-100wt%, wherein the content of norkurarinone, xanthohumol, kuraridin, and trifolirhizin occupies 1-100wt%.Alkaloid content in total alkaloid extract is 5-100wt%, wherein the content of matrine n-oxide, N-oxysophocarpine, matrine and sophocarpine occupies 5-100wt%.

The invention provides a veratramine degradation product veratrum fluorene aldehyde 1 and derivatives thereof. The invention uses steroidal alkaloid veratramine (A) as raw material and prepares veratrum fluorene aldehyde by oxidative degradation of m-chloroperoxybenzoic acid to obtain 20 derivative compounds of the veratrum fluorene aldehyde 1 after further oxidation, reduction and condensation reaction. The 20 compounds of the invention have good inhibitory activity on cancer cells, wherein the compounds 1, 2, 5, 8, 13 and 17 have good inhibitory activity on cells of human pancreatic cancer cells BxPC-3 and SW1990, small-cell lung cancer cells NCI-H446, human colorectal cancer LOVO and the like, and the inhibitory activity of the compound veratrum fluorene aldehyde 1 is the most significant.

The invention provides a total alkaloid of common fibraurea stems, which contains palmatine hydrochloride with the weight percentage content over 60 percent and jatrorrhizine hydrochloride with the weight percentage content more than 10 percent. The invention also provides a method for preparing the total alkaloid of common fibraurea stems, fibriuretinin and jatrorrhizine. The method can be adopted to prepare the total alkaloid of common fibraurea stems and to extract separate fibriuretinin and jatrorrhizine, wherein if dry products are calculated on the basis of anhydrous palmatine hydrochloride (C21H22O4N.HCl), the fibriuretinin has the weight percentage content higher than 98.0 percent and is high in purity. The extraction-separation method enables the transfer rate of the palmatine hydrochloride to be up to over 26 percent and is 1.7 times higher than the prior conventional process (about 15 percent). The extraction process also maintains the jatrorrhizine while raising the transfer rate of the palmatine hydrochloride serving as a main component of the fibriuretinin, thereby greatly improving the resource utilization rate of medicinal materials. The process adopts a resin purification method, is simple and feasible in process flow, capable of regenerating and repeatedly utilizing resin and reducing environmental pollution caused by lye compared with the conventional salting-out method.

The invention provides Gegenqinlian extractive which is obtained by mixing four medicines of kudzuvine roots, baikal skullcap roots, goldthread roots, and licorice roots in the weight ratio of 8: 3: 3: 2, extracting effective parts and refining. The extractive has the main effective parts in the following weight percentage: total isoflavone of the kudzuvine roots is 13.5 to 21.0 percent, total flavone of the baikal skullcap roots is 20.5 to 32.5 percent, total alkaloid of the goldthread roots is 10.5 to 18.5 percent, and glycerrhizic acid is 2.0 to 5.0 percent. Through adding medicinal auxiliary materials into the extractive, and adopting a conventional method, various peroral preparation for treating bacillary dysentery, acute enteritis, and infantile virus diarrhea can be prepared.

The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.

The present invention discloses a method for extracting total alkaloids in semen strychni, and belongs to the technical field of drugs. The method comprises the steps of extracting semen strychni crude powder with acid, filling on a column, separating, and purifying to remove some of strychnine. The prepared semen strychni total alkaloid content is above 50%, wherein the content ratio of alkaloids and strychnine is about 2:1, thereby effectively reducing the toxicity and improving the safety of clinical medication. The extraction method can efficiently separated high purity semen strychni total alkaloids from the traditional Chinese medicine of semen strychni.

The invention discloses a total alkaloid of Stephania delavayi Diels, the content of which is measured by tetrahydropalmatine. Tetrahydropalmatine occupies 55-95 percent of the total alkaloid extract of Stephania delavayi Diels, wherein the weight percentage of tetrahydropalmatine contained is no less than 2 percent, while the weight percentage of palmatine hydrochloride contained is no less than 0.8 percent. The invention also discloses a preparation method of the total alkaloid of Stephania delavayi Diels, which relates to the steps as follows: adding the Stephania delavayi Diels meal into ethanol for diacolation or reflux to acquire alcohol extract, decompressing and recycling the ethanol and concentrating into a condensed ointment for dissolving and filtrating with acid to acquire a filtrate, passing the filtrate through a macroporous resin column or a strong acid cation exchange resin column for adsorption, washing with water to remove the impurities and extracting with a eluent, collecting the eluent and decompressing and recycling the extract and then concentrating and drying, thus acquiring the total alkaloid of Stephania delavayi Diels; the steps can also be decompressing a percolating liquid to recycle the ethanol, concentrating, dissolving with acid and then filtrating, adjusting the pH value of the filtrate with weak base to produce deposition, filtrating the deposition to acquire a filter residue, adding ethanol into the filter residue to resolve and decompress, recycle the ethanol and then drying, thus acquiring the total alkaloid. The results of a pharmacological study on the acquired total alkaloid show that the total alkaloid of Stephania delavayi Diels has good effect on spasmolysis, pain relieving, antiphlogosis and antibiosis, blood activating and so on, and can combine with the acceptable components in pharmacy to prepare various sustained release or controlled release formulation or other formulations.

The invention discloses a method for obtaining purified total alkaloid of strychnos nux-vomica by removing partial strychnine in the total alkaloid of the strychnos nux-vomica, which comprises the following steps: extracting with a certain concentration of ethanol, dissolving the extract with hydrochloric acid ; adjusting the pH value to be alkaline after dissolving; carrying out extracting and pressure reduction on dichloromethane to remove solvent to obtain the total alkaloid of the strychnos nux-vomica; ultrasound dissolving total alkaloid powder of strychnos nux-vomica with ethanol; carrying out pressure reduction and concentration to a certain volume; centrifuging the concentrated solution, taking supernate, refrigerating and standing to remove precipitate; and drying the supernate to obtain the purified total alkaloid of strychnos nux-vomica. The content of strychnine in the total alkaloid of strychnos nux-vomica extracted by the method is remarkably reduced; and in addition, the method has safe extraction process, low cost and is beneficial to industrial scale production. Proved by the evaluation result of a pharmacological test, the effects of analgesia, anti-inflammation, immunity and antitumor activity of the purified total alkaloid of strychnos nux-vomica are remarkably superior to the effects of total alkaloid of strychnos nux-vomica and the toxicity is remarkably reduced.

The invention discloses a method for separating total alkaloid from sophora flavescens ait by using ion exchange resin, which belongs to the field of Chinese medicament. The method comprises the following technical schemes: taking Chinese medicament extract, adjusting the pH value to 1-7, carrying out filtration, passing the extract through a cation exchange resin column, washing the extract by using deionized water until the pH value reaches 5-7, soaking the resin column in saline solution at appropriate concentration; eluting the extract by using saline solution containing ethanol until no alkaloid is detected, collecting the eluent, adding alkali to the eluent for neutralization, and obtaining Chinese medicament total alkaloid after desalination, concentration and drying. The method overcomes the disadvantages of low extraction efficiency, large consumption of organic solvent, excessively high acid and alkali concentration, complicated technique, unavailable mass production and thelike in the prior art. Moreover, the method can efficiently separate high-purity Chinese medicament total alkaloid from the Chinese medicament extract.

The invention provides a preparation method for climbing nightshade extract which comprises, steeping medicinal powders with edible ethanol, decompression reclaiming ethanol and compensating the soak, charging natural clarifying agent so as to precipitate the impurity, finally drying the filtered clean liquid into block-shaped solid extract, wherein the major component of the extract is steroidal alkaloid with anti-inflammation action, which can be prepared into electuary for treating eczema and fungus skin diseases.

The invention discloses a corydalis saxicola bunting alkaloid extract and a preparation method thereof, as well as an extraction method of dehydrocavidine on the basis the alkaloid extract. According to the method, in the raw material pretreatment, pectinase and cellulose perform enzyme hydrolysis to the medicament, so that the cellulose component and pectin component in plant tissues are destroyed, the solubility of effective components is improved, and the content of total alkaloids in the extract is greatly increased. The content of the total alkaloids extracted by using a twice chromatography method is over 95 percent, and only ethanol is used as a solvent; and the dehydrocavidine monomer is extracted from the total alkaloids by adopting a high-speed counter-current chromatography technology, and the purity is over 99 percent.

The invention relates to the technique for separating and purifying effective parts from Lotus Plumule, and specifically discloses a separation and enrichment method of total flavones and total alkaloids of Lotus Plumule. According to the separation and enrichment method, alkaloids and flavones of Lotus Plumule are separated in a same separation unit in a centralized way and then separately eluted and enriched with different eluents. The separation and enrichment method comprises the following specific steps of: carrying out ethanol / water extraction on Lotus Plumule used as the raw material and removing impurities with a clarifying agent; enriching and separating the purified liquid with macroporous weak-acid cation exchange resin, eluting with ethanol / water to obtain total flavones, concentrating the eluate to be dry and drying to obtain total flavones of Lotus Plumule; and then eluting with NaCl-containing ethanol / water to obtain the total alkaloids, concentrating the eluate to be dry, removing salts and drying to obtain the total alkaloids. The separation and enrichment method provided by the invention is simple, feasible and environmentally-friendly, and has the characteristics of low cost, high yield of total flavones and total alkaloids; and by the adoption of the method, Lotus Plumule resources can be saved greatly, and the pharmaceutical utilization rate of Lotus Plumule can be improved.

The invention relates to new medical application of a fructus evodiae total alkaloid, namely, the application of the fructus evodiae total alkaloid in the preparation of a medicament for preventing and treating hepatic fibrosis. The fructus evodiae total alkaloid is a general name of indole-quinoline and quinolone alkaloids extracted and separated from fructus evodiae, and the content of the total alkaloid is between 20 and 60 percent. The fructus evodiae total alkaloid shows a certain treating effect in a therapeutic action experiment on rat hepatic fibrosis induced by carbon tetrachloride and has an excellent clinical application prospect. The fructus evodiae total alkaloid has an exact anti-hepatic fibrosis function and can be combined with auxiliary materials for preparing an anti-hepatic fibrosis medicament.

The application and preparing process for the common alkaloids of coptis root, phellodendron bark and berberis root are disclosed. Said common alkaloid is prepared from its raw material through extracting and drying. They can be used to prepare the medicines for treating diabetes and hyperlipemia.

The invention discloses application of a transcription factor involved in regulating tomato steroid alkaloid synthesis. The amino acid sequence of the transcription factor SlbHLH114 is as shown in SEQID NO.4, and the nucleotide sequence is as shown in SEQ ID NO.3. Overexpression of the transcription factor has a positive regulating effect on the synthesis of tomato steroid alkaloids, so the steroid alkaloid content of transgenic lines can be improved by overexpressing the transcription factor SlbHLH114 in plants, and a theoretical basis is provided for tomato quality improving and excellent breeding.

The invention discloses a novel process for extracting and purifying alkaloids of Sophora alopecuroides, which is simple, feasible, reasonable and suitable for industrial production. The process comprises the following steps: taking Sophora alopecuroides seeds as raw materials, extracting the total alkaloids of the Sophora alopecuroides by the ethanol impregnation and ultrasonic method, extracting the total alkaloids with an organic solvent, and refining the total alkaloids by the silica gel chromatography. When the active ingredients are purified by utilizing the method, transfer rate of the active ingredients is high and the loss of the effective ingredients is less, thereby achieving the purposes of reducing the production cost and improving the economic benefit.

The invention discloses a solanum lyratum extract, and an anticancer medicine comprising the solanum lyratum extract. The solanum lyratum extract is prepared through steps that: a solanum lyratum medicinal material is extracted by using ethanol, wherein the solanum lyratum medicinal material is processed through ethanol refluxing extraction, the recovered solvent is evaporated until dried, such that an ethanol extract is obtained; the extract is refined, wherein the ethanol extract is dissolved in water to form a solution, the solution is absorbed by using a weak acidic cation exchange macroporous resin column and is orderly eluted by using ethanol and hydrochloric ethanol, the hydrochloric ethanol eluent is collected and is neutralized by using ammonia water, and the collected product isdesalted, such that the solanum lyratum extract is prepared. The solanum lyratum extract contains 1% to 60% of steroid alkaloid. According to the invention, weak acidic cation exchange macroporous resin is adopted, steroid alkaloid is specifically accumulated from various components of solanum lyratum. The component of accumulated steroid alkaloid is simple. As a result of experiments, the substance has a substantial anticancer potency, and the anticancer potency is greatly higher than that of solanum lyratum aqueous crude extracts and steroid saponins extracted by using n-butyl alcohol in prior arts.

The invention relates to a method for preparing total alkaloid from macleaya cordata and further separating the total alkaloid to obtain high content alkaloid, particularly a preparation method for high content sanguinarine and chelerythrine. The preparation method comprises: atomizing and extracting a macleaya cordata raw material by acid water, adsorbing and eluting an extraction liquid by weakly polar macroporous resin, and recovering a solvent from an eluant and drying the eluant to obtain the total alkaloid, the content of which is not less than 60%; dissolving the total alkaloid in low-degree ethanol, carrying out secondary adsorption by using polar macroporous resin, carrying out stepwise elution by using 60% and 80% ethanol, and recovering a solvent from an eluant and drying the eluant to separately obtain the chelerythrine and the sanguinarine, the contents of which are not less than 90%. The preparation method provided by the invention is simple in process step, short in production period, high in product yield and less in use level of solvent, the environment is slightly polluted, and the obtained product is high in content, stable in quality and low in cost.

The invention relates to a preparation method for traditional Chinese medicine alkaloid salt and application thereof, in particular to a preparation method for fumitory total alkaloids salt and application of the total alkaloids salt as a medicament for treating cardiovascular diseases. The method solves the problem of poor effect of treating the cardiovascular diseases due to poor water solubility and low bioavailability of the fumitory total alkaloids salt. The preparation method for the fumitory total alkaloids salt comprises the following steps: extracting and purifying fumitory coarse powder to obtain the fumitory total alkaloids, and then carrying out salt-forming reaction between the fumitory total alkaloids and acid to obtain the fumitory total alkaloids salt. The extraction ratio of the fumitory total alkaloids is more than 0.5 percent, and the purity of the obtained fumitory total alkaloids salt is more than 50 percent. The fumitory total alkaloids salt has the advantages that the fumitory total alkaloids salt has good water solubility, has a solubleness more than 60 percent, has high bioavailability, and is used as an effective component for treating the cardiovascular diseases, such as coronary heart disease, angina pectoris, arrhythmia and premature beat.

The invention discloses a method for preparing total alkaloids from bamboo reeds for controlling the growth of harmful algae, which belongs to the technical field of water pollution control. The method comprises the steps of: cleaning bamboo reed plants, performing drying treatment on the cleaned bamboo reed plants, crushing the dried bamboo reed plants, and then adding the crushed bamboo reed plants into acid water for soakage; fully soaking the crushed bamboo reed plants, and then obtaining an extracting solution after decoction and reflux extraction; filtering to remove residue in the extracting solution, and performing alkalization treatment on the extracting solution until a floccule or a precipitate is produced to obtain an alkalified solution A; then using CHCl3 to extract the alkalified solution A, and separating to obtain an aqueous phase B and an organic phase C; and removing a solvent in the organic phase C to obtain an extract D, and then dissolving the extract D into ethanol to obtain the total alkaloids E from bamboo reeds for algae control. The method has the characteristics of simple process, wide material sources, low cost, cheap and non-toxic reagents for the preparation and the like, and solves the problem of secondary pollution or high preparation cost existing in the prior chemical algicide or algae inhibitor. At the same time, the prepared total alkaloidsfrom bamboo reeds have the advantages of good algae-inhibiting effect, definite ingredients, controllable quality, good safety and higher purity.

The invention relates to a method for extracting and purifying total alkaloid of Chinese mahonia stem; wherein the total alkaloid can be used to prevent and treat gastric ulcer, is mainly composed of berberine hydrochloride, jatrorrhizine hydrochloride, palmatine hydrochloride, and other alkaloids, and can be solely used or cooperatively used with pharmaceutically-acceptable carriers to prepare drugs for preventing and treating gastric ulcer.

The invention discloses a method for preparing a total alkaloid from daphniphyllum calycinum. The method comprises the following steps of: smashing daphniphyllum calycinum serving as a raw material; alkalifying by using an appropriate amount of saturated lime water for 2-3 hours, putting into a supercritical extracting kettle, introducing liquid CO2 and an entrainer, extracting under the pressure of 15-35MPa and at the temperature of 40-50 DEG C for 1-3 hours; resolving an extract under the pressure of 5-10MPa and at the temperature of 40-60 DEG C; recovering a reagent from the extract; adding an appropriate amount of acid water for dissolving; filtering, and adding into macroporous resin for adsorbing; washing a resin column with water; collecting a water washing liquid and a lower column liquid; adjusting to neutral; concentrating into an extract under reduced pressure; dissolving with a 80 percent ethanol; filtering; concentrating to a small volume, and crystallizing; and filtering a crystal out, and drying to obtain the total alkaloid. The method has the advantages of high efficiency, short period, low pollution and high yield, and is suitable for industrial production.

The present invention includes novel compounds based on the tomatidine skeleton as well as composition comprising these compounds alone and in combination with known compounds, which exhibit antimicrobial activity against extracellular or intracellular electron transport-deficient microbes and / or increase the antimicrobial activity of aminoglycoside antibiotics against their targets, and which are useful as antibacterial agents for treatment or prophylaxis of monomicrobiotic or polymicrobic bacterial infections or for the reduction of antibiotic resistance development in animals or in humans, or for use as antiseptics or agents for sterilization or disinfection.

A Zuojin alkaloid used for preparing different medicines is prepared from coptis root and evodia fruit through breaking, mixing, adding methanol hydrochloride, thermal reflux, filtering to obtain theliquid extract, decocting the dregs, filtering, concentrating to obtain liquid, mixing two kinds of liquid together, deposition, pumping filter, recovering methanol, adding alumina, mixing, drying, thermal reflux extracting with alcohol, crystallizing, filtering and drying. Its advantages are high purity and high feasibility.