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Process for extracting and purifying total alkaloids of Sophora alopecuroides

A technology of total alkaloids and Sophora fragrans, applied in digestive system, drug combination, plant raw materials and other directions, can solve the problems of cumbersome process, complicated process and high production cost

Inactive Publication Date: 2010-07-07
BEIJING HOPE HUGE PHARM SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the cumbersome process of these methods, the extraction time is long, which affects the yield of active ingredients
In addition, there are many types of alkaloids in Sophora sophora, and their structures and properties are close, which brings certain difficulties to separation and purification.
At present, the practical technology for systematically extracting, separating and purifying alkaloids from Sophora alopecuroides is still immature, especially the production of alkaloid monomers, which has high production costs and complicated processes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Extraction process of Sophora sophorae total alkaloids

[0018] Accurately weigh 1Kg of bitter bean seeds, crush them and divide them into 10 parts, use 55%-85% ethanol, the pH of the extraction solvent is 0.3-1.1, the reflux time is 1-5h, the ultrasonic treatment time is 5-30s, the ultrasonic frequency Extract at 28kHz and 45kHz respectively, filter the extract and evaporate the filtrate to dryness, make a 5.00ml solution of the residue with absolute ethanol, measure it with bromothymol blue colorimetry, calculate the matrine content and extract the matrine Rate.

[0019] Accurately take 10 parts of bitter bean powder, each part of 1.00g, use ethanol as the extraction agent, based on the results obtained from the above-mentioned single factor experiment, determine the four factors of ultrasonic intensity, extraction agent concentration, ultrasonic time, and pH of the solution. The levels and value ranges of each factor are carried out by orthogonal desig...

Embodiment 2

[0021] Embodiment 2: Extraction process of Sophora sophorae total biological alkaloids

[0022] The pH is 10-11, and 6 solvents of different polarities, petroleum ether, diethyl ether, chloroform, ethyl acetate, acetone, and n-butanol, are used for 3-8 extraction experiments on the decolorized alkaloid extract, and the The extract phase was taken out and checked by thin-layer chromatography. Determine the extraction process. The best process is: pH is 10.5, using chloroform as solvent, after 6 times of extraction, the extraction rate of alkaloid has reached and tended to be stable.

Embodiment 3

[0023] Embodiment 3: Purification process of Sophora sophora total biological alkaloids

[0024] The column separation and elution condition of the total alkaloids of Sophora flavescens is to continuously increase the proportion of methanol in chloroform for elution. Use V (chloroform): V (methanol) = 9: 1 gradient elution method to elute the silica gel column; then use V (chloroform): V (methanol) = 8.5: 1.5 for elution; then use V (chloroform) : V (methanol) = 8:2 elution. Crystallization with acetone, the detection results were matrine, oxymatrine. And have reached 98% purity.

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Abstract

The invention discloses a novel process for extracting and purifying alkaloids of Sophora alopecuroides, which is simple, feasible, reasonable and suitable for industrial production. The process comprises the following steps: taking Sophora alopecuroides seeds as raw materials, extracting the total alkaloids of the Sophora alopecuroides by the ethanol impregnation and ultrasonic method, extracting the total alkaloids with an organic solvent, and refining the total alkaloids by the silica gel chromatography. When the active ingredients are purified by utilizing the method, transfer rate of the active ingredients is high and the loss of the effective ingredients is less, thereby achieving the purposes of reducing the production cost and improving the economic benefit.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, and discloses an extraction and purification process of total biological alkaloids of Sophora sophorae, in particular the extraction and separation of two monomers, matrine and sophorine, in the total alkaloids. Background technique [0002] Bitter beans, belonging to the genus Sophora in the family Fabaceae, are widely distributed in Northwest China. Its active ingredient - alkaloids - has important pharmacological activities and application prospects in anti-tumor, anti-bacterial, anti-arrhythmia, anti-hepatitis B, immunity, etc. The pharmacological effects of alopecia areata alkaloids mainly include: [0003] (1) Anti-tumor effect Matrine in the alkaloid can significantly inhibit the human erythroleukemia cell line K562 cells from entering the S phase, inhibit its DNA replication, and inhibit its proliferation ability; it has an inhibitory effect on the growth and proliferation of h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61P1/16A61P9/00A61P11/06A61P29/00A61P31/04A61P31/12A61P35/00A61P37/08
Inventor 王翰斌
Owner BEIJING HOPE HUGE PHARM SCI
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