The invention discloses a
microwave-assisted aqueous two-phase / reversed micelles extraction method of
Sophora flavescens alkaloids. The method comprises the steps of extracting Radix Sophorae Flavescentis or Radix Sophorae Tonkinensis by use of
microwave-assisted
ethanol-
ammonium sulfate aqueous two-phase
system, in which
Sophora flavescens alkaloids are partitioned between the two aqueous phases and extracted into the upper phase to complete primary purification; selectively extracting
Sophora flavescens alkaloids by use of SDBS (
sodium dodecyl
benzene sulfonate) / isooctane / n-
octanol reversed micelles
system, to obtain
Sophora flavescens alkaloids crude product with low
impurity content; and performing separation and purification to obtain
matrine,
oxymatrine and sophoridine. The method combines reversed micelles extraction,
microwave-assisted extraction and aqueous two-phase extraction technologies, to realize
advantage complementation, simple method, high speed and efficiency, no
pollution, low requirements for facilities, easy
continuous production and scale-up. The extracted
Sophora flavescens alkaloids have purity above 90.92% and yield up to 55.38 mg / g. The separated and purified
matrine,
oxymatrine and sophoridine have high purity above 94% and little
organic solvent residue.