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Method for preparing general flavone and total alkaloid of sophora flavescens simultaneously

A technology of total flavonoids and total alkaloids of Sophora flavescens, applied in the field of medicine

Inactive Publication Date: 2007-12-12
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the method of simultaneously extracting and separating the total flavonoids extract and the total alkaloid extract of Sophora flavescens

Method used

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  • Method for preparing general flavone and total alkaloid of sophora flavescens simultaneously
  • Method for preparing general flavone and total alkaloid of sophora flavescens simultaneously
  • Method for preparing general flavone and total alkaloid of sophora flavescens simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0067] Example 2: Preparation process of Sophora flavescens total flavonoids extract and total alkaloids extract

[0068] Sophora flavescens dried root 1kg, 70% ethanol 12L, reflux extraction 3 times, extract 1.5 hours each time, recover solvent under reduced pressure, obtain extract, add water to disperse (solution concentration is 30mg / mL), centrifuge (3000rpm, 30min), obtain Aqueous solution and residue, add 20 times the amount of 98% ethanol to the residue for ultrasonic dissolution, centrifuge (3000rpm, 30min) to obtain the residue and ethanol solution, recover the solvent from the ethanol solution until it has no alcohol smell, add 12 times the amount of 0.7% HCl for ultrasonic dissolution, and centrifuge (3000rpm, 30min) to obtain the residue and acidic aqueous solution, wash the residue to neutrality, and dry under reduced pressure to obtain the total flavonoids extract of Sophora flavescens. Combine the acidic water and the aqueous solution, adjust to neutral with 2mo...

Embodiment 3

[0069] Example 3: Preparation process of Sophora flavescens total flavonoids extract and total alkaloids extract

[0070]Sophora flavescens dried root 1kg, 70% ethanol 14L, reflux extraction 3 times, extract 1 hour each time, recover solvent under reduced pressure, obtain extract, add water to disperse (solution concentration is 25mg / mL), centrifuge (3000rpm, 30min), obtain Aqueous solution and residue, add 15 times the amount of 99% ethanol to the residue and ultrasonically dissolve, centrifuge (3000rpm, 30min) to obtain the residue and ethanol solution, recover the solvent from the ethanol solution until it has no alcohol smell, add 9 times the amount of 0.5% HCl to dissolve it ultrasonically, centrifuge (3000rpm, 30min) to obtain the residue and acidic aqueous solution, wash the residue to neutrality, and dry under reduced pressure to obtain the total flavonoids extract of Sophora flavescens. Combine the acidic water and the aqueous solution, adjust neutral concentration wi...

Embodiment 5

[0073] Embodiment 5: preparation of Sophora flavescens total flavonoids extract tablet

[0074] Sophora flavescens total flavonoids extract 100g

[0075] Starch 100g

[0076] The above components are mixed evenly, and an appropriate amount of talcum powder is added, and pressed into 1000 tablets.

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Abstract

The invention discloses a method for simultaneously preparing total flavone extract and total alkaloid extract of Radix sophorae flavescentis. Total flavone extract mainly comprises flavanones (flavanonols) such as norkurarinone, kurarinone, and isokurarinon and other flaconoids such as trifolirhizin, xanthohumol, and kuraridin. Total alkaloid extract mainly comprises matrine n-oxide, N-oxysophocarpine, matrine, sophocarpine, sophoridine, and sophoramine. Total flavone extract and total alkaloid extract can be extracted through one or more of methods of solvent-extraction, solvent extraction, macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Flavonoid content in total flavone extract is 5-100wt%, wherein the content of norkurarinone, xanthohumol, kuraridin, and trifolirhizin occupies 1-100wt%.Alkaloid content in total alkaloid extract is 5-100wt%, wherein the content of matrine n-oxide, N-oxysophocarpine, matrine and sophocarpine occupies 5-100wt%.

Description

technical field [0001] The invention belongs to the technical field of medicine. In particular, it relates to a preparation method and a quality control method for simultaneously preparing the total flavonoid extract and the total alkaloid extract of Sophora flavescens and its application in the fields of pharmaceuticals, health care products and cosmetics. Background technique [0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a plant of the genus Sophora in the leguminous family. It has the effects of clearing away heat and dampness, killing insects and diuresis. Sophora flavescens contains a large amount of flavonoids and alkaloids, which have antibacterial, insecticidal, antiviral, and anti-inflammatory effects, and are the main material basis for the medicinal effects of flavescens. At present, there is no report on the method of simultaneously extracting and separating the total flavonoid extract and the total alkaloid extract of Sophora flavesc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61K8/97A61P17/00A61P9/06A61P29/00A61P31/04A61P31/12A61Q19/06C07D311/30C07D455/00A61K125/00
CPCY02P20/54
Inventor 刘斌石任兵朱丽君
Owner 石任兵
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