Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of total alkaloid of Chinese mahonia stem and applications of total alkaloid of Chinese mahonia stem in preparation of drugs for preventing and treating gastric ulcer

A technology of total alkaloids and kung fu wood, which is used in pharmaceutical combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc.

Inactive Publication Date: 2014-07-23
何欣
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, there are only 74 domestic researches on Gongluomu at present, mainly on the chemical components of Gongluomu, the extraction of a single component, alkaloids, and alkaloids (such as berberine hydrochloride, palmatine hydrochloride and jatrorrhizine hydrochloride). However, there are no related reports and patents on the treatment and prevention of gastric ulcer by the single gonglao wood, its chemical components and its effective parts.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of the total alkaloids of embodiment 1 Gongluo wood

[0026] Weigh 100g of hardwood powder, add 1200ml of 50% ethanol, reflux extraction at 90°C, filter, recover ethanol to 200ml, then reflux extraction with 2% sulfuric acid, filter, adjust PH8-9, stand still, filter to obtain the filtrate After adding 15% sodium chloride, adjust the pH to 1-2, filter, wash the precipitate with water, and dry at 80°C. Dissolve the precipitate in distilled water, then extract it with 2% sulfuric acid under reflux, adjust the filtrate to pH 8-9, let stand, filter, Obtain filtrate, after adding 15% sodium chloride, regulate PH1-2, filter, precipitation washing, 80 ℃ of dryings, obtain purity and be 52.12% (wherein the content of jatrorrhizine hydrochloride, palmatine hydrochloride, and argonine hydrochloride are respectively 21.89%, 2.72%, 27.51%) of the total alkaloids.

Embodiment 2

[0027] The preparation of the total alkaloids of embodiment 2 Gongluo wood

[0028] Weigh 100g of broad-leaved eucalyptus powder, add 1200ml of 50% ethanol, reflux extraction at 90°C, filter, recover ethanol to 200ml, then extract with 2% sulfuric acid, filter, adjust PH8-9, stand still, filter to obtain the filtrate, Add sodium chloride at 15%, stir to dissolve, add hydrochloric acid, adjust PH1-2, filter, wash the precipitate with water, dry at 80°C to obtain extract, dissolve this extract in distilled water, take equal parts, and put them on D113 and On the HPD100 resin column, distilled water and 10% ethanol remove impurities, and 50% acid alcohol elutes to obtain a purity of 67.94% respectively (wherein the contents of jatrorrhizine hydrochloride, palmatine hydrochloride, and jatrorrhizine hydrochloride are respectively 40.11%, 16.58% %, 11.26%) and 59.55% (wherein the contents of jatrorrhizine hydrochloride, palmatine hydrochloride, and quinine hydrochloride are 31.22%, ...

Embodiment 3

[0029] The preparation of the total alkaloids of embodiment 3 Gongluo wood

[0030] Weigh 150g of broad-leaved eucalyptus powder, add 1800ml of 50% ethanol, reflux extraction at 90°C, filter, recycle ethanol to 300ml, then extract with 2% sulfuric acid solution, filter, adjust pH to 8-9 with ammonia water, stand still, and filter to obtain The filtrate was adjusted to PH1-2, PH3-4, and PH5-6 with hydrochloric acid, respectively, and was respectively placed on the macroporous resin HPD100. First, the impurity was removed with distilled water, and then eluted with 30% ethanol. The recovered eluent had a purity of 44.79% respectively. % (wherein the contents of jatrorrhizine hydrochloride, palmatine hydrochloride, and quinine hydrochloride are respectively 22.14%, 11.91%, and 10.74%), 43.25% (wherein the contents of jatrorrhizine hydrochloride, palmatine hydrochloride, and quinine hydrochloride The total alkaloid content is 21.54%, 11.94%, 10.22%), 43.90% (wherein the contents of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting and purifying total alkaloid of Chinese mahonia stem; wherein the total alkaloid can be used to prevent and treat gastric ulcer, is mainly composed of berberine hydrochloride, jatrorrhizine hydrochloride, palmatine hydrochloride, and other alkaloids, and can be solely used or cooperatively used with pharmaceutically-acceptable carriers to prepare drugs for preventing and treating gastric ulcer.

Description

Technical field: [0001] The invention relates to the preparation of total alkaloids of gonglao wood and the application of the total alkaloids and its pharmaceutical composition in medicines for preventing and treating gastric ulcer. Background technique: [0002] Gastric ulcer is a common clinical disease and one of frequently-occurring diseases. It is a digestive system disease with many complications, slow healing and easy recurrence. The incidence rate is increasing year by year. In terms of epidemiology, peptic ulcer is a common disease, and its incidence rate varies greatly in different periods and regions, and the total incidence rate accounts for about 10-20% of the population. About 10% of patients with peptic ulcer disease in the United States, 12.3% in Germany; According to a survey in a few areas in my country, the incidence of digestive tract diseases is 11.43%, of which the incidence of peptic ulcer is 4.54%. The pathogenesis of gastric ulcer is complex and ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/29A61K31/4375A61P1/04
Inventor 何欣
Owner 何欣
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com