Method for extracting purified total alkaloid of strychnos nux-vomica and application thereof in pharmacy
A technology of total alkaloids and extraction method, which is applied in the field of extraction and purification of total alkaloids of purifying St. Chin seeds, can solve problems such as high cost, difficulty in realizing large-scale industrial production, and large loss of alkaloid components, and achieves toxicity reduction, resistance Tumor pharmacological action, effect of easy industrial production
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Embodiment 1
[0029] Embodiment 1: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:
[0030] (1) take Nuxychia, pulverize, sieve, make coarse powder, take by weighing 200g coarse powder;
[0031] (2) Heat and reflux extraction with 70% ethanol, 1000 mL each time, extract for 1 hour, extract 3 times in total, filter, combine the filtrates three times, recover ethanol under reduced pressure, and obtain alcoholic extract;
[0032] (3) Sonicate with 400 mL of 1 mol / L hydrochloric acid, centrifuge at 4000 rpm for 10 min, and take out the supernatant;
[0033] (4) Regulate pH to 12 with sodium hydroxide, use 2000mL dichloromethane to extract and merge in 5 times, remove dichloromethane by vacuum drying, accurately weigh the weight to obtain the total alkaloid powder of St. The content of strychnine accounts for 52% of the total alkaloids of Nuxychnium;
[0034] (5) ultrasonically dissolving the total alkaloid powder of Nuxebra chinensis obtained above in 2000mL ...
Embodiment 2
[0037] Embodiment 2: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:
[0038] (1) take Nuxychia, pulverize, sieve, make coarse powder, take by weighing 200g coarse powder;
[0039] (2) Heat and reflux extraction with 90% ethanol, 2000 mL each time, extract for 2 hours, extract twice in total, filter, combine the filtrates, recover ethanol under reduced pressure, and obtain alcoholic extract;
[0040] (3) ultrasonically dissolve with 0.2mol / L hydrochloric acid 1000mL, and suction filter to obtain the filtrate;
[0041] (4) Use sodium hydroxide to adjust the pH to 14, use 1000mL of dichloromethane to extract and merge three times, recover under reduced pressure to remove the dichloromethane, accurately weigh the weight, and obtain the total alkaloids of Nuxica chinensis, which are determined by HPLC. Dinin content accounts for 46% of the total alkaloids of Strychnis;
[0042] (5) ultrasonically dissolving the total alkaloid powder of Nuxebra c...
Embodiment 3
[0045] Embodiment 3: the method for extracting and purifying the total alkaloids of Nuxebra chinensis:
[0046] (1) take Nuxynum, pulverize, sieve, make coarse powder, take by weighing 100g coarse powder;
[0047] (2) Heat and reflux extraction with 50% ethanol, 1000 mL each time, extract for 1 hour, extract 4 times in total, filter, combine filtrates, recover ethanol under reduced pressure, and obtain alcoholic extract;
[0048] (3) ultrasonically dissolve with 0.5mol / L hydrochloric acid 400mL, and suction filter to obtain the filtrate;
[0049] (4) Regulate the pH to 10 with sodium hydroxide, extract and combine in 4 times with 500mL dichloromethane, reclaim under reduced pressure to remove the dichloromethane, accurately weigh the weight, and obtain the total alkaloids of Nuxica chinensis, which are determined by HPLC The content of dinine accounts for 44% of the total alkaloids of Nuxebra chinensis;
[0050] (5) ultrasonically dissolving the total alkaloid powder of Nuxe...
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