56results about How to "Increase alkaloid content" patented technology

The invention discloses a culture method for effectively improving the total alkaloid content of Fritillaria cirrhosa D. Don, which is characterized in that: in the method, precursor-feeding technology is adopted, and in the liquid culture process of young regenerated bulbs of Fritillaria cirrhosa D. Don, the total alkaloid content of Fritillaria cirrhosa D. Don cultures is improved by adding amino acid precursors. The experimental results show that the total alkaloid content of final obtained Fritillaria cirrhosa D. Don cultures can reach 0.212 percent after 25-day culturing, which is 52.5 percent higher than that of the control group without adding amino acid precursors. The invention not only effectively improves the total alkaloid content of Fritillaria cirrhosa D. Don in liquid culture, but also significantly increases the reproductive rate of biomass of Fritillaria cirrhosa D. Don, thereby achieving the purpose of rapidly producing the effective ingredients of unibract fritillary bulbs better.

The invention discloses a davilpepper tryptophan decarboxylase protein coding sequence in the gene engineering domain, which is characterized by the following: coding the ribonucleotide sequence with 70% consanguinity with 154-1653rd in the SEQ ID NO.1; crossing 154-1653rd in the SEQ ID NO.1 under 45-55 deg.c.

The invention discloses a metabonomics and transcriptomics correlation analysis based method for screening a key gene for the synthesis of fritillaria alkaloid. Specifically, the differential alkaloidand the content information of the differential alkaloid are obtained through the metabonomics analysis of three types of fritillaria, and meanwhile, the expression condition of the related gene in the synthetic route of the alkaloid is obtained through the functional annotation of the transcriptomics. The differential metabolin-gene dependence relation network is constructed through the correlation analysis, and the key gene for the synthetic route of the alkaloid, having a greater contribution to the content of the alkaloid in the fritillaria, is screened. The method provides important information for the further clarification of the synthesis mechanism of the alkaloid substance in the fritillaria, and provides an important evidence for increasing the content of fritillaria alkaloid bymeans of gene control and the like.

The invention relates to the technical field of plant cultivation, in particular to a coptis teeta cultivation and fertilizing method. In the coptis teeta cultivation and fertilizing method, soil preparation, field planting, fertilizing and the like are adopted, nitrogenous fertilizer, phosphatic fertilizer and micro element fertilizer are applied in the growth period of coptis teeta, and plant growth and rhizome alkaloid generation are facilitated. The method has the advantages of being capable of obviously improving the yield and achieving great economic benefits. Local advantageous forestland resources can be fully utilized to develop the forest undergrowth industry, and obvious ecological benefits and economic benefits can be embodied on the premise that forest plants are less damaged. Soil nutrition conditions are changed through manual fertilizing, and sufficient nutrients are provided for growth of the coptis teeta. Microelements are additionally applied to promote synthesis of alkaloid, a high biological yield is guaranteed, a high alkaloid content is acquired at the same time, and quality of the coptis teeta is improved; meanwhile, the method is adopted based on field forest undergrowth cultivation, a growth environment the same as that of field coptis teeta is achieved, and product geoherbalism is guaranteed.

The invention discloses a method for increasing alkaloid content of radix sophorae tonkinensis tissue culture seedlings by utilizing selenium. The radix sophorae tonkinensis tissue culture seedlings are placed in a culture medium containing selenium for culture. The selenium element is added into the culture medium, so that the alkaloid content of radix sophorae tonkinensis is effectively increased, meanwhile the selenium absorption rate of plants is increased, and growth of radix sophorae tonkinensis tissue culture seedlings and accumulation of enzymes, hormones and main active ingredients inbodies can be adjusted.

The invention provides a rhizoma gastrodiae decoction piece and a preparation method thereof, and relates to the technical fields of traditional Chinese medicinal materials and processing thereof. Therhizoma gastrodiae decoction piece is prepared from the following raw materials: rhizoma gastrodiae, frankincense, myrrh, ephedra, pinellia ternate, radix achyranthis bidentatae and eucommia ulmoides. The rhizoma gastrodiae decoction piece provided by the invention is prepared from the main raw material of rhizoma gastrodiae in cooperation with frankincense, myrrh, ephedra, pinellia ternate, radix achyranthis bidentatae and eucommia ulmoides, the property of the traditional Chinese medicinal materials is smooth and is not hot and dry, according to the principle of monarch, minister, assistantand guide, the prepared decoction piece is low in water content and bright in color and luster, the content of the active ingredients such as gastrodin is high, the immune function can be improved when the decoction piece is taken at proper time, the decoction piece has obvious inhibition effect for early inflammatory exudation and expansion, and the decoction piece also has the effects of relieving cough, eliminating phlegm, promoting biliary secretion and the like.

The invention discloses an application of arabidopsis thaliana UGT74F2 in catalyzing phenyllactic acid to be subjected to glycosyl transfer reaction to synthesize phenyllactoyl glucose. Research finds that UGT74F2 from arabidopsis thaliana can catalyze phenyllactic acid to synthesize phenyllactoyl glucose, and the catalytic activity of the UGT74F2 is higher than that of AbUGT1 reported at present and used for tropine alkaloid synthesis. The UGT74F2 can be used for tropane alkaloid metabolic engineering to improve the alkaloid content, the application value of UGT74F2 is widened, and the UGT74F2 has very important significance on medicinal TA metabolic engineering and synthetic biology.

The present invention discloses a compound preparation for increasing an alkaloid content in dendrobium huoshanense. The preparation is prepared from the following raw material components in percentage by weight: 0.02-0.04% of ethephon, 0.01-0.03% of methyl jasmonate, 0.002-0.005% of tryptophan, 0.001-0.003% of lysine, 0.001-0.003% of citric acid, 0.002-0.004% of succinyl benzoic acid, 0.005-0.008% of potassium dihydrogen phosphate, 0.1-0.3% of an organosilicon additive and the balance of water. Dendrobium huoshanense seedling plants are sprayed with the compound preparation avoiding the rain in 20-30 days before dendrobium harvest every year, and the spary amount is 20-40 mL / m2, the preparation is sprayed once every 5-7 days for totally 2-4 times, continuously. By adopting the compound preparation disclosed by the present invention, the total alkaloid content in the dendrobium huoshanense can be remarkably increased.