56results about How to "Increase alkaloid content" patented technology

The invention discloses a culture method for effectively improving the total alkaloid content of Fritillaria cirrhosa D. Don, which is characterized in that: in the method, precursor-feeding technology is adopted, and in the liquid culture process of young regenerated bulbs of Fritillaria cirrhosa D. Don, the total alkaloid content of Fritillaria cirrhosa D. Don cultures is improved by adding amino acid precursors. The experimental results show that the total alkaloid content of final obtained Fritillaria cirrhosa D. Don cultures can reach 0.212 percent after 25-day culturing, which is 52.5 percent higher than that of the control group without adding amino acid precursors. The invention not only effectively improves the total alkaloid content of Fritillaria cirrhosa D. Don in liquid culture, but also significantly increases the reproductive rate of biomass of Fritillaria cirrhosa D. Don, thereby achieving the purpose of rapidly producing the effective ingredients of unibract fritillary bulbs better.

The invention discloses a method for producing bulbus fritillariae cirrhosae total alkaloid by adopting cell mass suspension culture. In the method, a bulbus fritillariae cirrhosae cell mass obtainedfrom induction culture is cut into a size of 3-4 mm and then grafted into a liquid culture medium for suspension culture, and finally a culture product which has high alkaloid content (which is 2.04 times that of market bulbus fritillariae cirrhosae bulb) and short growing period (which lasts only 24-28 days) is obtained. The method better solves the problems of long time, low crude drug yield, low effective component content and the like which exist in conventional bulbus fritillariae cirrhosae producing methods. In addition, the method can realize the aim of large scale and fast production of effective components of bulbus fritillariae cirrhosae crude drug.

The invention relates to an extraction and enrichment method of fritillaria total alkaloids, comprising the following steps of: first, taking fritillaria, extracting with hydrochloric acid with the mass fraction of 0.5-2%, concentrating the extracting solution, centrifuging the concentrated solution, taking the supernatant; second, regulating the pH of the supernatant to 8-11, extracting with dichloromethane, collecting the dichloromethane, recovering the solvent until dried to obtain the total alkaloid crude extract; and third, dissolving the total alkaloid crude extract with hydrochloric acid with the mass fraction of 0.5-2%, regulating the pH to 8-11, taking the loading solution, enabling the loading solution to pass through HPD100 type or HPD722 type macroporous resin columns, washing with water until the eluent is colorless and represents negative shown by sugar detecting reaction, eluting with ethanol with the volume fraction of 90-95% until the eluent represents negative shown by alkaloid detecting reaction, collecting the ethanol eluent, and recovering the solvent until dried to obtain the total alkaloid extract. By the extraction and enrichment method of fritillaria total alkaloids provided by the invention, the purity of the obtained fritillaria total alkaloids is high.

The invention discloses a method for extracting maca alkaloids through microwave and ultrasonic wave combination. The process comprises the main steps: grinding raw materials, mixing and dissolving, controlling the parameters, stirring through microwave and ultrasonic wave combination, filtering, and extracting. The method comprises the following sub-steps: mixing and dissolving the maca powder according to a material-liquid ratio of 1:60, preliminarily stirring the raw materials and distilled water in a stirrer, transferring the mixture into a built-in ultrasonic extraction tank, operating at normal temperature, and selecting ultrasonic waves in the wave band at the frequency of 40KHz, wherein the power of the ultrasonic wave is 300 W, the selected ultrasonic wave frequency is 300MHz, the microwave power is 352 W, and the stirring time is 30 minutes; evaporating and concentrating the extracting solution subjected to filtration, concentration, pH regulation and extraction so as to obtain the extract of the maca alkaloids.

The invention discloses a davilpepper tryptophan decarboxylase protein coding sequence in the gene engineering domain, which is characterized by the following: coding the ribonucleotide sequence with 70% consanguinity with 154-1653rd in the SEQ ID NO.1; crossing 154-1653rd in the SEQ ID NO.1 under 45-55 deg.c.

The invention discloses a co-fermented Sichuan pepper beer. 0.1-0.6 part of humulus lupulus is added into 100-200 parts of saccharified wort in batches to prepare wort, then, precipitation separationis conducted, 5-10 parts of Sichuan pepper nano-powder is added, after first-time sterilization, 0.1-0.3 part of commercially available beer yeast is added to carry out twice fermentation, and then sterilization is conducted again to obtain the co-fermented Sichuan pepper beer. The co-fermented Sichuan pepper beer is prepared by co-fermenting Sichuan pepper and beer, the wort is used for producingalcohol under the action of microorganisms, increasing of alcohol concentration facilitates changing and dissolving out of internal substances of the Sichuan pepper, and the solubility of some flavorsubstances and active substances in the Sichuan pepper can be gradually improved along with increasing of the alcohol concentration, so that the relative content of the characteristic flavor substances in the Sichuan pepper is improved, and a flavor stereoscopic feeling of the Sichuan pepper is enhanced. Through reasonable design and blending, the flavor of the Sichuan pepper and the flavor of the beer are fused and harmonized, and thus the co-fermented Sichuan pepper beer gives people comfortable and cheerful feelings.

The invention relates to the production technology of traditional Chinese medicines, and discloses a processing method of dendrobium officinale. The processing method comprises the following steps: soaking with hot water, freezing at top speed, softening at constant temperature, freezing for the second time, drying and performing thermal treatment; the surface of dendrobium officinale is golden yellow in color, faintly scent in smell; and the active ingredients of dendrobium officinale can be kept to the maximum extent. According to the processing method, the dendrobium officinale does not need to be fried, more loss of active ingredients, free polysaccharide, bonded polysaccharide and alkaloid, of the dendrobium officinale due to local overheating can be effectively avoided, and after being treated by the processing technology, the dendrobium officinale is not needed to be boiled, and can be eaten after being soaked directly with boiling water, thus being simple and convenient.

The invention discloses a rapid test method for adulteration of Chinese date honey, and relates to the technical field of ultraviolet spectrophotometry detection. The rapid test method uses absorbance of total alkaloids in the Chinese date honey as a measurement index and comprises the following steps: (1) preparation of a Chinese date honey sample solution : 1) extracting the total alkaloids from the Chinese date honey sample, and 2) dissolving; (2) preparation of a reference solution; (3) determination of absorbance: using an ultraviolet spectrophotometer to determine the absorbance of the Chinese date honey sample solution and the reference solution at the maximum absorption wavelength place of the Chinese date honey sample solution; (4) drawing of working curves and computing of regression equations; (5) computing of the adulteration amount of Chinese date honey sample: according to the regression equations in the step (4) and the absorbance of the Chinese date honey sample solution in the step (3), computing the natural Chinese date honey content in the Chinese date honey sample to obtain the adulteration amount of the Chinese date honey sample. The method is wide in applicability, simple to operate, cheap in the equipment price, low in the determination cost, and suitable for popularization and application.

The invention discloses a metabonomics and transcriptomics correlation analysis based method for screening a key gene for the synthesis of fritillaria alkaloid. Specifically, the differential alkaloidand the content information of the differential alkaloid are obtained through the metabonomics analysis of three types of fritillaria, and meanwhile, the expression condition of the related gene in the synthetic route of the alkaloid is obtained through the functional annotation of the transcriptomics. The differential metabolin-gene dependence relation network is constructed through the correlation analysis, and the key gene for the synthetic route of the alkaloid, having a greater contribution to the content of the alkaloid in the fritillaria, is screened. The method provides important information for the further clarification of the synthesis mechanism of the alkaloid substance in the fritillaria, and provides an important evidence for increasing the content of fritillaria alkaloid bymeans of gene control and the like.

The invention relates to the technical field of cultivation of Chinese herbal medicines, in particular to a dendrobium officinale planting method. The dendrobium officinale planting method comprises the following steps: soil preparation, matrix preparation, field planting, fertilization and watering. By use of a tailor-made sterilized solution, a tailor-made nutrient solution and a tailer-made base fertilizer, the nutrition demands required for growth of dendrobium officinale are met, and dendrobium officinale is long and thick in stem and good in tiller; and very good prevention effects are achieved on anthracnose, black spot, soft rot endangering stems, southern blight and stem rot, which lead to yield decrease of dendrobium officinale in the dendrobium officinale growth process, so that the leaf yield of dendrobium officinale per mu reaches 2,109.3 kg / hm<2>, and the stem yield of dendrobium officinale per mu reaches 1,703.1 kg / hm<2>. While the yield of dendrobium officinale is increased, the quality of dendrobium officinale is improved, the polysaccharide content is up to 33.1%, and the alkaloid content is up to 0.053%.

The invention discloses a fishing bait for fishing grass carps. The fishing bait comprises the following ingredients in parts by weight: 45 to 52 parts of puffing soybean powder, 40 to 50 parts of puffing sweet potato powder, 15 to 20 parts of wheat berry, 5 to 8 parts of sesame powder, 20 to 35 parts of high protein flour, 5 to 10 parts of Chinese chive juice, 10 to 18 parts of tomato juice, 15 to 22 parts of green corn juice, 2 to 4 parts of cheese, 4 to 8 parts of coffee powder, 5 to 9 parts of cinnamon powder, 2 to 5 parts of stramonium powder, 8 to 12 parts of almond powder, 18 to 25 parts of rice wine, 20 to 22 parts of white sugar, 5 to 15 parts of bean worm powder, 2 to 6 parts of yellow mealworm powder, 4 to 9 parts of shrimp powder and 5 to 10 parts of duck liver powder. The fishing bait for fishing grass carps provided by the invention has the advantages that the cost is low, the storage time is long, the fishing bait can help fishermen to lower the difficulty for trapping and fishing the grass carps, and in addition, the fishing bait cannot easily expand or disperse in water.

The invention relates to the technical field of plant cultivation, in particular to a coptis teeta cultivation and fertilizing method. In the coptis teeta cultivation and fertilizing method, soil preparation, field planting, fertilizing and the like are adopted, nitrogenous fertilizer, phosphatic fertilizer and micro element fertilizer are applied in the growth period of coptis teeta, and plant growth and rhizome alkaloid generation are facilitated. The method has the advantages of being capable of obviously improving the yield and achieving great economic benefits. Local advantageous forestland resources can be fully utilized to develop the forest undergrowth industry, and obvious ecological benefits and economic benefits can be embodied on the premise that forest plants are less damaged. Soil nutrition conditions are changed through manual fertilizing, and sufficient nutrients are provided for growth of the coptis teeta. Microelements are additionally applied to promote synthesis of alkaloid, a high biological yield is guaranteed, a high alkaloid content is acquired at the same time, and quality of the coptis teeta is improved; meanwhile, the method is adopted based on field forest undergrowth cultivation, a growth environment the same as that of field coptis teeta is achieved, and product geoherbalism is guaranteed.

The present invention discloses one kind of soft capsule of tonkin sophora root total alkaloid. The soft capsule consists of two parts, inclusion and covering. The inclusion includes Tonkin sophora root total alkaloid, dispersing medium and stabilizer; and the covering includes gelatin, glycerin, water and preservative. The present invention also discloses the preparation process. The preparation process is simple, and the soft capsule of tonkin sophora root total alkaloid has high stability, controllable quality, easy taking and high antitumor effect.

The present invention discloses a compound preparation for increasing an alkaloid content in dendrobium huoshanense. The preparation is prepared from the following raw material components in percentage by weight: 0.02-0.04% of ethephon, 0.01-0.03% of methyl jasmonate, 0.002-0.005% of tryptophan, 0.001-0.003% of lysine, 0.001-0.003% of citric acid, 0.002-0.004% of succinyl benzoic acid, 0.005-0.008% of potassium dihydrogen phosphate, 0.1-0.3% of an organosilicon additive and the balance of water. Dendrobium huoshanense seedling plants are sprayed with the compound preparation avoiding the rain in 20-30 days before dendrobium harvest every year, and the spary amount is 20-40 mL / m2, the preparation is sprayed once every 5-7 days for totally 2-4 times, continuously. By adopting the compound preparation disclosed by the present invention, the total alkaloid content in the dendrobium huoshanense can be remarkably increased.

The invention discloses a method for increasing alkaloid content of radix sophorae tonkinensis tissue culture seedlings by utilizing selenium. The radix sophorae tonkinensis tissue culture seedlings are placed in a culture medium containing selenium for culture. The selenium element is added into the culture medium, so that the alkaloid content of radix sophorae tonkinensis is effectively increased, meanwhile the selenium absorption rate of plants is increased, and growth of radix sophorae tonkinensis tissue culture seedlings and accumulation of enzymes, hormones and main active ingredients inbodies can be adjusted.

The invention belongs to the field of traditional Chinese medicine processing, and provides a method for processing the ephedra decoction pieces. The method includes the steps of: (1) preparing a mainingredient; (2) preparing an auxiliary material; and (3) processing with honey. The content of alkaloid in the decoction pieces obtained by the existing ephedra processing method by ginger and licorice can be obviously decreased, and the additional ability of the ephedra processed with honey is not obvious. The method adopts ginger, licorice and pinellia as the auxiliary material, pre-microbial fermentation is carried out, then the materials and honey are fried with the ephedra decoction pieces, which not only increases the content of alkaloids in the ephedra decoction pieces, the total amount of alkaloids is increased by at least 31%, and the efficacy of ephedra to relieve cough and asthma is enhanced.

The invention discloses a preparation method and medical application of an extract of monkshood reduced total alkali, which comprises the following steps of: adding inorganic alkali in a monkshood water extraction process, and keeping the pH value of liquid medicine to be 9-12 alkalinity in a decoction reflux process, so that aconitine is converted into a new compound during extraction; a combined process of a macroporous resin refining process, impurity elution with a diluted alcohol solution, impurity elution with an acid water solution and component elution with an acidic diluted alcohol solution is adopted, diluted alcohol is an ethanol solution with the concentration of 20% or below, the process has the unexpected effects that the production safety is improved, the cost is reduced, and the prepared extract is high in alkaloid content and safer in production. The invention is composed of a process of converting an aconitine structure into a new compound through reflux extraction of a strong alkali solution and a process of macroporous resin refining and diluted alcohol elution, the prepared main components are the new compound, the ecognin and the 15-hydroxyl ecognin, the content of the ecognin and the 15-hydroxyl ecognin accounts for more than 50% of the total alkali, and the invention is low in toxicity and strong in drug effect and is used for preparing drugs for treating inflammation pain.

The invention provides a rhizoma gastrodiae decoction piece and a preparation method thereof, and relates to the technical fields of traditional Chinese medicinal materials and processing thereof. Therhizoma gastrodiae decoction piece is prepared from the following raw materials: rhizoma gastrodiae, frankincense, myrrh, ephedra, pinellia ternate, radix achyranthis bidentatae and eucommia ulmoides. The rhizoma gastrodiae decoction piece provided by the invention is prepared from the main raw material of rhizoma gastrodiae in cooperation with frankincense, myrrh, ephedra, pinellia ternate, radix achyranthis bidentatae and eucommia ulmoides, the property of the traditional Chinese medicinal materials is smooth and is not hot and dry, according to the principle of monarch, minister, assistantand guide, the prepared decoction piece is low in water content and bright in color and luster, the content of the active ingredients such as gastrodin is high, the immune function can be improved when the decoction piece is taken at proper time, the decoction piece has obvious inhibition effect for early inflammatory exudation and expansion, and the decoction piece also has the effects of relieving cough, eliminating phlegm, promoting biliary secretion and the like.

The invention relates to a Strobilanthes cusia BcTSA gene, protein encoded by the same and application of the Strobilanthes cusia BcTSA gene. The nucleotide sequence of the Strobilanthes cusia BcTSA gene is as shown in SEQ ID No. 1, and the nucleotide sequence of the protein encoded by the Strobilanthes cusia BcTSA gene is as shown in SEQ ID No. 2. The BcTSA gene is separated from Strobilanthes cusia, cloning and biological information analysis of the Strobilanthes cusia BcTSA gene is completed, and the understanding of indole alkaloid biological synthesis is perfected. The Strobilanthes cusiaBcTSA gene can control indole alkaloid synthesis, the protein encoded by the Strobilanthes cusia BcTSA gene is applicable to the indole alkaloid synthesis and used for increasing the yield of indolealkaloid, and a high-yield and stable plant material is provided for the large-scale production of the indole alkaloid.

The invention relates to a method for producing an aroma enhanced and bitter removed lotus leaf beverage through enzymolysis. The method comprises the following steps of: (1) weighing lotus leaf powder, adding a certain quantity of water, blending uniformly, extracting in a water bath, performing microwave extraction, and filtering to remove residue; (2) degassing under normal pressure, and adjusting the pH value; (3) adding lotus seed flavor enzyme, performing enzymolysis reaction in the water bath, and filtering with heat to remove residue; and (4) flavoring: adding vitamin C and sucrose into lotus leaf beverage, filtering, sterilizing, cooling, filling, and thus obtaining the aroma enhanced and bitter removed lotus leaf beverage. Through sensory evaluation and index testing on the obtained beverage, the lotus leaf beverage with strong aroma and gentle smell is obtained. According to the method, functional substances in lotus leaves are extracted, so that the quality of the beverage is greatly improved; and through further treatment by the flavor enzyme, the beverage has thick, pure and soft aroma and smell and has good market prospect and economic benefits.

The invention discloses a method for planting wild dendrobe on rocks. The method comprises the following steps of (1) conducting rock treatment, selecting the rocks, respectively using a flame sprayerand a sodium chloride solution for treating, punching surfaces of the rocks, and spraying a sterilizing solution; (2) growing seedlings, rooting, in March, selecting wild dendrobe seedlings with lengths of about 1cm, transplanting into a greenhouse nursery bed, and filling a sponge pad with a rooting solvent to grow the seedlings at a constant temperature; (3) laying, taking planting soil, addingrock powder and guar powder, mixing, laying on the surfaces of the treated rocks, and using a plastic mesh for covering; (4) planting, transplanting planting dendrobe seedlings to the planting rocks,fixing the seedlings with cloth strips, and spraying the planting soil on the upper parts of the roots of the dendrobe seedlings with a guar solution; (5) conducting daily management, laying a greenhouse on a planting area, setting a shade net, and controlling factors such as temperature, humidity, shading rate and the like in the greenhouse.

The invention discloses a method for establishing a transgenic system of a high-sugar variety beet. The method comprises the steps of: beet seed germination, beet seedling growth, agrobacterium infection, gene identification or resistance screening, etc. The method for transgenic beet provided by the invention provides a technical support for basic research depending on a transgenic technology related to improvement of the important alkaloid content of the beet and the ornamental quality and the economic value of the beet, and has important reference significance for establishment of transgenic methods of plants of beet genus.

The invention provides a method for increasing the content of alkaloid in lycoris radiata through an exogenous substance, and relates to the field of plant growth and secondary metabolite accumulation regulation. According to the method for increasing the content of the alkaloid in the lycoris radiata through the exogenous substance, 3-month-to-one-year-old tissue culture seedlings of the lycoris radiata are treated through the exogenous substance 4 '-O-methyl norlinaldine and / or jasmonic acid methyl ester, so that the content of three alkaloids, that is, galanthamine, lycorine and lycorine which have important medicinal values in the lycoris radiata are effectively increased.

The invention discloses a preparation method of tropane alkaloids in anisodus tanguticus, which comprises the following steps: preparing and crushing anisodus tanguticus, adding an acetic acid-methanol solution for extraction, filtering the extract by a ceramic membrane to remove impurities, separating the extract by an organic membrane to remove impurities, and drying the extract the water solution penetrating through the organic membrane to obtain the anisodus tanguticus alkaloids. The preparation method is high in extraction efficiency, convenient to operate and beneficial to industrial production, popularization and application.

The invention discloses an application of arabidopsis thaliana UGT74F2 in catalyzing phenyllactic acid to be subjected to glycosyl transfer reaction to synthesize phenyllactoyl glucose. Research finds that UGT74F2 from arabidopsis thaliana can catalyze phenyllactic acid to synthesize phenyllactoyl glucose, and the catalytic activity of the UGT74F2 is higher than that of AbUGT1 reported at present and used for tropine alkaloid synthesis. The UGT74F2 can be used for tropane alkaloid metabolic engineering to improve the alkaloid content, the application value of UGT74F2 is widened, and the UGT74F2 has very important significance on medicinal TA metabolic engineering and synthetic biology.

The invention discloses a production method of traditional Chinese medicine fresh herba leonuri. With the production method, a three-season off-season cultivation mode is established, a high-yield regulation model is established, and the characteristics of high alkaloid content, stability and good uniformity of the fresh herba leonuri are simultaneously established. The fresh herba leonuri plantedby the method has dwarfed plant shape, multiple tillers, multiple and thick blades; normal bolting, flowering and fruiting cannot occur; and the fresh herba leonuri is fresh herba leonuri capable ofproviding high yield and high alkaloid content in ten months of a year and having stability and good uniformity.

The present invention discloses a compound preparation for increasing an alkaloid content in dendrobium huoshanense. The preparation is prepared from the following raw material components in percentage by weight: 0.02-0.04% of ethephon, 0.01-0.03% of methyl jasmonate, 0.002-0.005% of tryptophan, 0.001-0.003% of lysine, 0.001-0.003% of citric acid, 0.002-0.004% of succinyl benzoic acid, 0.005-0.008% of potassium dihydrogen phosphate, 0.1-0.3% of an organosilicon additive and the balance of water. Dendrobium huoshanense seedling plants are sprayed with the compound preparation avoiding the rain in 20-30 days before dendrobium harvest every year, and the spary amount is 20-40 mL / m2, the preparation is sprayed once every 5-7 days for totally 2-4 times, continuously. By adopting the compound preparation disclosed by the present invention, the total alkaloid content in the dendrobium huoshanense can be remarkably increased.

The invention discloses a method for effectively increasing the alkaloid content of pinellia ternate through low-phosphorus treatment. In the pinellia ternate planting process, pinellia ternate tissue culture seedlings are treated through a low-phosphorus culture solution, and the method for rapidly and effectively increasing the alkaloid content of the pinellia ternate is established. The pinellia ternate tissue culture seedlings induce the expression of related genes (PtCNL, PtCHD, PtKAT, PtPDC, PtAO4 and PtAHAS) of a pinellia ternate alkaloid synthetic pathway under the premise of not influencing the growth and development of the pinellia ternate tissue culture seedlings subjected to short-term low-phosphorus treatment, so that the alkaloid content of the tuber of the pinellia ternate is increased. The method is easy to implement and remarkable in effect, and powerful reference is provided for increasing the alkaloid content of the pinellia ternate and improving the quality of the pinellia ternate.

The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method of high-content coptis chinensis powder. Coptis chinensis powder is prepared by sorting, drying, slicing and fibrous root removing of coptis chinensis, soaking with jujube water, pre-freezing and vacuum drying, and crushing. The rhizoma coptidis powder obtained by the preparation method provided by the invention overcomes the problems of high ash content, more scorched parts, low alkaloid content and the like of rhizoma coptidis powder obtained by a traditional process, and the obtained rhizoma coptidis powder is high in alkaloid content, high in yield and good in taste.

The invention provides Korean monkshood root diterpene total alkaloids and a preparation method thereof and specifically discloses a method for preparing diterpene total alkaloids from Korean monkshood. According to the method, a CO2 supercritical extraction method is used to extract Korean monkshood root, then acidification-alkalization-extraction treatment is carried out, and obtained extract is washed with alkaline water. Specifically speaking, the method comprises the following steps: step 1, taking the medicinal material--the Korean monkshood root and subjecting the Korean monkshood root to supercritical extraction with CO2 as a solvent; step 2, dissolving the extract obtained in the step 1 with acid water, subjecting an obtained acid water solution to alkalization and then carrying out extraction with an organic solvent; and step 3, washing the organic solvent extract obtained in the step 2 with alkaline water so as to recover the organic solvent. The content of the total alkaloids is as high as more than 50%, and the content of the poisonous component--Guanfu base H is greatly decreased.