361 results about "Gastrodin" patented technology

The invention relates to a gastrodin synthesizing method, which can effectively solve the preparation problem of gastrodin to meet the requirements of the gastrodin in pharmaceuticals. The method comprises the steps of adding catalyst perchloric acid, acetylating anhydrous dextrose by using acetic anhydride to produce per-acetyl dextrose, feeding hydrogen bromide to bromizing hemiacetal hydroxyl of the per-acetyl dextrose to produce bromo-tetraacethyl glucose, further and dropwise adding a bromo-tetraacethyl glucose solution into chloroform and tetrabutyl ammonium bromide, carbonate and para hydroxybenzene in water to obtain 4-formyl benzene-2', 3', 4', 6'- tetraacetyl-beta-D-glucopyranose, performing re-crystallization with ethanol, adding raney nickel or palladium and carbon, feeding hydrogen and pressurizing to hydrogenate, performing filtering, adding sodium alcoholate or ammonia in to filtrate to perform protecting group removal until the reaction is finished completely, performing pressure reduction and concentration to obtain crude gastrodin, and re-crystallizing the crude gastrodin by using alcohol or an alcohol and ester solvent or an alcohol and ketone solvent to obtain the gastrodin. The gastrodin synthesizing method is abundant and cheap in raw materials, simple in process, recycled in solvent, small in pollution and high in quality.

The invention discloses a black green or green black gastrodia elata cultivating method. The black green or green black gastrodia elata cultivating method comprises the following steps: breeding first-generation selfing black gastrodia elata and first-generation selfing green gastrodia elata; controlling to achieve flower synchronization of the gastrodia elata, and hybridizing respectively to breed black green or green black gastrodia elata seeds; and performing bionic planting or field planting on the black green or green black gastrodia elata seeds to breed the required black green or green black gastrodia elata. The black green or green black gastrodia elata with high gastrodin content and high yield is bred by selfing and hybridizing the black gastrodia elata and the green gastrodia elata with relatively high gastrodin contents respectively.

The invention discloses a method for chemically synthesising gastrodin, comprising the following steps of: in the presence of a molecular sieve, under the catalysis of Lewis acid, and performing glycosylation reaction on pent-acetyl-b-D-glucose and p-cresol in an organic solvent with to generate 4-methylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside; then preparing 4-halomethylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside from 4-methylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside and N-halosuccinimide in the presence of an initiator, and then reacting 4-halomethylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside with the mixed solution of glacial acetic acid and tertiary amine to obtain 4-acetoxylmethylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside; and finally removing the acetyl protecting group from the 4-acetoxylmethylphenyl-2,3,4,6-O-tetra-acetyl-b-D-glucopyranoside in an alkaline condition to obtain gastrodin. Compared with the traditional method, the method disclosed by the invention has easily available raw materials, and is short in reaction time, capable of preparing the reaction product in each step via recrystallization, simple, and more suitable for industrialized production for gastrodin.

The invention discloses an artificial wild gastrodia elata cultivation technique. The technique includes the following steps: collecting local wild fresh armillaria mellea mycelia, preparing primary mother strains in a test tube, and switching secondary solid strains; selecting loose loam, sandy loam good in drainage performance or sandy soil, wherein pH value of the soil is 5-6; preparing firewood for preparing mushroom sticks and mushroom branches; preparing a filling material; culturing the mushroom sticks; cultivating gastrodia elata. A mushroom stick culturing method and a gastrodia elata cultivating method are more suitable for growing of gastrodia elata; especially, in the cultivating process of the gastrodia elata, sophora flavescens powder or tobacco stem powder is utilized, so that the mycelia can be prevented from being eaten by insects; penicillin liquid is sprayed, so that mushroom contaminating and rotting can be prevented; the artificial cultivation technique of the gastrodia elata is enabled to be more scientific and more reasonable, and yield of the gastrodia elata is stable; product detecting shows that gastrodin content is 0.38%, and product requirements are met completely.

The invention discloses a bionic organic cultivation method of rhizoma gastrodiae under a forest, belonging to an agricultural technology. The bionic organic cultivation method can be used for overcoming the defect that the medical quality of the rhizoma gastrodiae is reduced due to the fact that the rhizoma gastrodiae drug loses original wild characters caused by excessive effects of personal factors in an existing box cultivation method, a pit cultivation method, a half-pit cultivation method and a pile cultivation method for production of the rhizoma gastrodiae. The bionic organic cultivation method comprises the following steps: selecting a proper geographical position and an environment condition, making a seedbed, seeding, transferring and managing in the field and harvesting fruits. The bionic organic cultivation method has the beneficial effects that the produced fresh rhizoma gastrodiae is large and thick, intact and plump, yellow and white; the section is bright after the rhizoma gastrodiae is processed; the quality is hard, and the characters of the wild rhizoma gastrodiae are reflected. By efficient liquid-phase monitoring, the gastrodin content is 0.301mg / ml and higher than the standard that the content of the gastrodin in 'Pharmacopoeia of the People's Republic of China' (2005 edition) prepared according to the existing box cultivation method, pit cultivation method, half-pit cultivation method and pile cultivation method is 0.20mg / ml; the yield is higher than or equal to that of the existing cultivation method; forest land and waste resources of wood are fully utilized, and the bionic organic cultivation method plays a certain protection role on the ecological environment.

The invention relates to the field of medicines, and particularly relates to a gastrodin compound. The gastrodin compound provided by the invention is a crystal, and the X-ray powder diffraction obtained via a measurement adopting a Cu-K(alpha) ray, of the gastrodin compound, shows characteristic peaks at places having angles 2*theta of 9.4 degrees, 9.9 degrees, 15.1 degrees, 15.6 degrees, 18.4 degrees, 22.5 degrees, 25.6 degrees, 27.9 degrees, 28.6 degrees, 29.1 degrees, 30.5 degrees, 30.9 degrees, 31.5 degrees, 32.6 degrees, and 32.7 degrees+ / -0.2 degrees. The gastrodin compound is high in lattice energy and good in stability, thus greatly improving medication safety. Additionally, the invention further provides a preparation method for the gastrodin compound, and a medicine composition containing the gastrodin crystal. The gastrodin medicine composition provided by the invention is good in storage stability and higher in safe performance.

Relating to the medicine field, the invention discloses the use of acetagastrodine in preparing medicines to prevent and treat vascular dementia and Alzheimer disease. The invention also provides a medicinal composition for preventing and treating senile dementia and its capsule as well as sustained release agent. Animal tests show that: acetagastrodine can substantially enhance the learning and memory abilities of rats with vascular dementia, reduce the activity of AchE in brains, enhance the vitality of ChAT in brains, and remarkably reduce Glu content; acetagastrodine has significant protective effect on damaged PC12 cells caused by H2O2 by enhancing the vitality of SOD and total ATP enzyme in PC12 cells and reducing the content of MDA and LD; acetagastrodine can substantially enhance the learning and memorizing abilities of AD model rats, enhance the CAT content in brains of the model rats, reduce the MAO vitality and simultaneously prevent the decrease of neurotransmitters. Acetagastrodine is better than gastrodin in terms of the treatment effects on VD and AD, and enjoys good clinical application prospect.

The invention relates to a gastrodin ferulate compound and a preparation method and application of the gastrodin ferulate compound. The problem that gastrodin and ferulic acid are prepared into the gastrodin ferulate compound and the problem of preparation of medicine for improving and treating neurasthenia, neurasthenia syndrome and angioneurotic headache are effectively solved. The method comprises the following steps: carrying out acetylization on anhydrous dextrose by using acetic anhydride; carrying out hemiacetal hydroxyl bromination, and then condensing with p-hydroxy benzaldehyde; carrying out hydride reduction to obtain acegastrodine; carrying out hydroxyl acetylated protection on ferulic acid to obtain 3-(4'-acetyl-3'-methoxyphenyl)-2-acrylic acid, and then preparing cetylferuloyl chloride; dissolving acegastrodine into a drying solvent and adding a nucleophilic acylation catalyst and an acid-binding agent; dissolving cetylferuloyl chloride into the drying solvent; mixing the two sollutions to obtain an ester compound; and carrying out deacetylation under an alkaline condition, thus obtaining the gastrodin ferulate compound. The gastrodin ferulate compound disclosed by the invention has the efficacies of calming mind, mesmerizing, and improving blood circulation of the brain.

A process for preparing the whole powder of fresh gastrodia tuber includes such steps as fast squeezing the fresh gastrodia tuber to obtain its juice, solvent extraction of residual dregs, mixing them, and instanteneous spray-drying. Its advantages are full extraction of active components, short period, low cost, high solubility and high content of gastrodin.

The Chinese medicine composition is used in treating dizziness, nausea and vomiting, especially Meeniere's syndrome, and has the functions of eliminating phlegm, preventing dizziness, regulating the middle warmer and stopping vomiting. It is prepared with 12 kinds of Chinese medicinal materials, including gastrodia tuber, hooked uncaria, oriental water plantain, pinellia tuber, etc. and contains also beta-cyclodextrin as volatile oil including agent, beta-cyclodextrin as co-solvent for liposoluble component, aspartame as corrective and dextrin as excipient. The quality control method during the preparation process includes thin layer identification of hooked uncaria, white peony root, Chuanxiong rhizome and licorice, and measurement of gastrodin in gastrodia tuber as main material and paeoniflorin in white peony root.

The invention discloses glycosyl transferase synthesized by catalytic gastrodine as well as a gene of encoding enzyme and application. The glycosyl transferase synthesized by catalytic gastrodine is shown in SEQ ID No:1; another glycosyl transferase synthesized by catalytic gastrodine is shown in SEQ ID No:2. According to the glycosyl transferase synthesized by two catalytic gastrodine, p-hydroxybenzyl alcohol can be catalyzed and converted into gastrodine, so that the yield of gastrodine can be obviously improved.

The invention discloses glycosyl transferase for catalyzing synthesis of gastrodin or salidroside, a gene coding the glycosyl transferase and application of glycosyl transferase. The glycosyl transferase for catalyzing synthesis of gastrodin or salidroside is as shown in SEQ ID No.1. Experiments prove that the glycosyl transferase for catalyzing synthesis of gastrodin or salidroside can be used for respectively catalytically converting hydroxy-benzyl alcohol into gastrodin and converting tyrosol into salidroside, and the yield of gastrodin and salidroside can be remarkably improved.

The invention discloses a preparation process of a tall gastrodia tuber tablet rich in gastrodin. The preparation process comprises the following steps of: selecting fresh tall gastrodia tuber, cleaning and cutting up; steaming with saturated steam of which the temperature is 100-120 DEG C for 5-10 hours; and drying at the temperature of 50-80 DEG C to obtain the tall gastrodia tuber tablet rich in gastrodin. The tall gastrodia tuber tablet prepared with the method disclosed by the invention tastes bitter but slightly sweet, and has high gastrodin content; and compared with the conventionally-processed tall gastrodia tuber, the tall gastrodia tuber tablet has the advantages of higher medical health-care value and commercial value.

The invention relates to a purification method of gastrodin. The method comprises the following specific steps: (1) using an alcohol-containing solution to perform ultrasonic extraction on gastrodin medicinal herb powder, collecting extract, concentrating to ensure that the extract has no alcohol; and (2) purifying a concentrated solution with polyamide resins, adopting ethanol-water (0-60%) to perform gradient elution, collecting the target eluent, concentrating to reach a small volume, adopting the dry method to add the concentrated target eluent with a small volume into an octadecylsilyl (ODS) silica gel column, eluting with an ethanol-water mixed solvent or an acetonitrile-water mixed solvent, recycling the solvent from the eluent, and drying to obtain gastrodin. The method has the advantages of low energy consumption, short preparation period and high product purity.

The invention discloses recombinant escherichia coli for utilizing glucose to produce p-hydroxybenzyl alcohol or gastrodin and application. The recombinant escherichia coli for utilizing glucose to produce the gastrodin comprises two expression vectors of pCDFDuet-aroG*-ppsA-pgm-galU and pETDuet-ubiC-CAR-Sfp-ugt73b6FS; the aro-G* gene is represented by SEQ ID NO:1. According to the invention, by constructing a new p-hydroxybenzyl alcohol synthetic pathway and regulating and controlling metabolic flux from glucose to tyrosine, the escherichia coli with high yield of gastrodigenin and p-hydroxybenzyl alcohol is obtained; yield of p-hydroxybenzyl alcohol reaches 240mg / L; a high-efficiency UPD glucosyltransferase mutant is introduced; biosynthetic pathways of gastrodin and p-hydroxybenzyl alcohol are organically combined together; yield of gastrodin is obviously improved; the highest yield of gastrodin can reach 265mg / L.

The present invention discloses rhizoma gastrodiae noodles and a preparation method thereof. The rhizoma gastrodiae noodles consist of the following components in parts by weight: 95-105 parts of wheat flour, 2.95-3.05 parts of rhizoma gastrodiae powder, 36-38 parts of water, 0.98-1.02 parts of salt, and 0.2 part of edible base. The rhizoma gastrodiae noodles are prepared from each component under the recipe as follows: 95-105 of wheat flour, 2.95-3.05 of rhizoma gastrodiae powder, 36-38 of water, 0.98-1.02 of salt, and 0.2 of edible base. The sensory evaluation of the rhizoma gastrodiae noodles is up 89.6, so that the rhizoma gastrodiae noodles have good flavor and mouthfeel. The steaming of the rhizoma gastrodiae noodles in consumption can cover unpleasant flavor and stink odor of the rhizoma gastrodiae. Due to the fact that the fresh rhizoma gastrodiae is rich in active ingredients of gastrodin, vanillin, proteins, amino acids, trace elements, etc. the prepared rhizoma gastrodiae noodles from wheat flour and rhizoma gastrodiae powder at a ratio of 100 : 3 ratio has significant effects of preventing epilepsy, palpitation and rheumatism, calming mind, relieving muscular spasm and pains, supplementing deficiency, calming liver and endogenous wind, relieving convulsion, etc. The rhizoma gastrodiae noodles have medicinal value, are soft and refreshing in mouthfeel during drinking, effectively solve the problem that the rhizoma gastrodiae medicinal wine is stimulus in mouthfeel, and have health-care functions of rhizoma gastrodiae and good flavor of the noodles.

The invention discloses core-shell SiO2@rosin-based polymer microspheres and a preparation method thereof. A rosin-based crosslinking agent, an acrylic monomer and a radical initiator are uniformly mixed, the mixture is uniformly applied to the surface of silica gel, the silica gel is placed in an aqueous phase for radical polymerization, and the core-shell SiO2@rosin-based polymer microspheres are obtained. The core-shell SiO2@rosin-based polymer microspheres have particle size distribution centralized in the range of 2-900 mu m, the mean pore size of 0.5-100 nm and the specific surface areaof 50-350 m<2> / g and have the characteristics of excellent degree of sphericity, uniform particle size, rich pore structure, high mechanical strength and high solvent tolerant degree, and a chromatographic column filled with the core-shell SiO2@rosin-based polymer microspheres as chromatographic packing especially has good separation effect on alkaloids, gastrodin and derivatives thereof.

The invention relates to a preparation method of nanometer silicon gel surface gastrodin molecular engram polymers, which belongs to the technical field of biochemical engineering. The preparation method of the invention comprises the following steps: dissolving template molecules of gastrodin and functional monomers of alpha-methacrylic acid into hole forming agents of methanol; sufficiently mixing the materials under the conditions of constant temperature and light shielding to obtain mixed liquid; adding silanization nanometer silicon gel particles, cross linking agents and catalytic amount triggering agents into the mixed liquid; carrying out repeated ultrasonic treatment after sufficient and uniform mixing; introducing nitrogen gas for deoxygenation; sealing the materials; carrying out reaction for 6h in water bath of 50 DEG C; then, carrying out reaction for 24h at 60 DEG C to obtain solid polymers; adopting a Sohxlet extraction method for extracting the solid polymers for more than 48h; carrying out elution for removing template molecules; drying the polymers till the constant weight after the extraction is completed; and obtaining the silicon gel surface gastrodin molecular engram polymers. The molecular engram polymers prepared by the invention have specific identification capability and selective absorption performance on the gastrodin. Compared with ordinary absorping agents, the molecular engram polymers prepared by the invention have the advantages of accuracy, high speed and sensitivity.

The invention provides a processing method for gastrodia elata decoction pieces. The processing method comprises the following steps: cutting a gastrodia elata raw material into slices, drying the slices with a first microwave tunnel, then drying with a drying box, and finally drying with a second microwave tunnel to obtain the gastrodia elata decoction pieces. The processing method belongs to the technical field of processing of traditional Chinese medicines. The processing method provided by the invention can realize rapid curing and drying of gastrodia elata, the processing cycle is greatly shortened, the production efficiency is improved, the processing method is easy to control, and the production energy consumption is reduced; the obtained gastrodia elata decoction pieces are low in water content, bright in luster, high in content of active ingredients, such as gastrodin, free of a cavitation phenomenon, few in surface wrinkles and unlikely to break.

The invention provides a method for extracting gastrodin by an enzyme method. The method is characterized by comprising the following steps: dissolving rhizoma gastrodiae powder in acetic acid-sodium acetate buffer solution and mixing the two substances evenly; adding complex enzyme into the solution for stirring evenly and enzymolysis; adding alcohol into the mixture to for sufficiently stirring, filtering and deslagging; concentrating extracting solution into extract by a rotary steaming method; and drying the extract to obtain the gastrodin extract. The method has the advantages that as high efficiency and specificity of biological enzyme and mild acting condition, compared with an ultrasonic extracting process, the extracting condition is more convenient, and the equipment is more simplified, so that energy consumption is reduced, and the cost is saved; compared with a hot alcohol refluence extracting process, the extracting condition is simple, the temperature of an extracting system is reduced, so energy consumption of heating is reduced, the pollution to the environment is reduced, a micromolecular drug effect ingredient with thermal volatilization can be remained, and the drug effect of the obtained gastrodin is more complete; and compared with the prior art, the extracting rate is improved by 0.05 to 0.13 percent.

The invention discloses a pharmaceutical composition for improving the safety of a compound gastrodin injection. The pharmaceutical composition is mainly prepared from gastrodin extract, extract of chuanxiong rhizome, safflower extract and Solutol HS-15 for injections. According to the pharmaceutical composition for improving the safety of the compound gastrodin injection, cosolvent of polysorbate 80 which can cause potential safety hazards and affect product quality is replaced with cosolvent with better safety and a more obvious hydrotropy effect in the compound gastrodin injection, the safety of the Solutol HS-15 is higher than that of the polysorbate 80, the dosage of the Solutol HS-15 is lower than that of the polysorbate 80, the occurrence probability of untoward effects and risks of pharmaceuticals are reduced, and the safety of clinical medication is improved.

The invention relates to a quality control method for a rhizoma gastrodiae capsule and belongs to the technical field of biomedicine. The quality control method comprises the steps of raw material control, preparation method control, an identification method and a content measurement method. Modern drug production management is integrated in quality control. The quality of drugs and solvents used in production is examined from the source. Product quality control in the production process is established, perfect product quality standards are established, the thin-layer chromatographic detection ratio is higher than national requirements, the gastrodin content is greatly increased, the increase degree reaches 28.3%, and the quality and the therapeutic effect of the drug product are ensured.

The invention discloses a rhizoma gastrodiae glucosyltransferase gene. The nucleotide sequence of the rhizoma gastrodiae glucosyltransferase gene is shown as SEQID NO:1, and an amino acid sequence asshown in SEQID NO:2 is coded. A glucosyltransferase gene sequence is screened out according to a CDS sequence provided by a rhizoma gastrodiae transcriptome, a nest type PCR primer is designed, and through amplification and splicing, an overall length sequence of the glucosyltransferase gene is obtained; a prokaryotic expression vector pGEX4T-UGT is constructed, proteins are obtained through expression and purification, a eukaryotic expression vector is constructed, subcellular localization is performed on expression of a UGT gene, glucosyltransferase is transferred into monokaryon honey mushroom mycelia by an agrobacterium tumefaciens infection method for the constructed eukaryotic expression vector pH2GW7.0 35S-UGT, transgene honey mushrooms are obtained, biosynthetic gastrodin is used,the rhizoma gastrodiae is artificially cultured by the transgene honey mushrooms, the content of gastrodin in the rhizoma gastrodiae is increased, and besides, the gastrodin can also be produced through culturing genetic engineering honey mushrooms.

The invention relates to a quality control method of rhizoma gastrodiae, a decoction piece thereof and rhizoma gastrodiae extract. Based on high-performance liquid chromatography, by the aid of a one-standard multi-measurement analysis method, the contents of gastrodin components of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C and parishin E in the rhizoma gastrodiae arecalculated by relative correction factors, the low-cost gastrodin with stable property serves as an internal standard substance, and six effective components in the rhizoma gastrodiae are simultaneously measured. The multi-index quality control method is high in detection sensitivity, rapid in analysis and good in stability, can objectively, comprehensively and accurately evaluate the quality of the rhizoma gastrodiae, the decoction piece thereof and the rhizoma gastrodiae extract, is of great significance for ensuring the curative effect of the rhizoma gastrodiae and can solve the problems that parishin component reference substances are unstable, high in price and the like, and the quality of the rhizoma gastrodiae, the decoction piece thereof and the rhizoma gastrodiae extract cannot becomprehensively controlled.

The invention discloses an application of gastrodin to preparing medicaments for preventing and treating Alzheimer's disease. Alzheimer's disease (AD) is a neurodegenerative disease, and pathological characteristics comprise neuro fibrillary tangles formed in cells, hyperplasia of reactive microglia and glial cell, and the like, wherein the extracellular deposition of amyloid A beta is the main pathogenetic reason of AD. An AD small mouse taking a gastrodin-containing fodder is substantially improved in spatial-memory learning ability, substantially reduced in A beta level in brain and serum, and substantially reduced in amyloid plaques and microglia in brain tissue, and thus it is indicated that gastrodin is capable of preventing A beta deposition and decreasing active components causing AD mouse brain gastrodin.

The invention discloses a method for extracting gastrodin. The method comprises the following steps: percolating gastrodia elata powder by a water-alcohol mixed solvent under the ultrasonic effect; loading percolation liquid to a neutral alumina chromatographic column, eluting by a methanol solution, and collecting gastrodin-section elution liquid; loading the percolation liquid to a macroporous resin chromatographic column, eluting by an alcohol-ester mixed solvent, and collecting gastrodin-section elution liquid; steaming, concentrating, and drying to obtain gastrodin extract. The gastrodin extract obtained by the method is high in purity, and the gastrodin extraction rate is high.

The invention discloses a gastrodin injection and a preparation method thereof. The gastrodin injection comprises gastrodin which is an active constituent, an accessory and water for injection. A prescription process is simplified through a large number of experiments, and the accessory only consists of buffer salt which has the effects of pH regulation and stabilization. The gastrodin injection does not contain organic solvents, auxiliary solvents, antioxidants and metal ion complexing agents, thereby improving the safety of clinical medicines and reducing the cost. Proved by pharmacological experiments, the gastrodin injection does not have anaphylaxis, irritant reactions and hemolytic reactions and can guarantee the medicine safety. The gastrodin injection has the advantage of solving the problems of reduced content and unstable pH value of the gastrodin injection in the process of storage, has simple prescription, good stability, low production cost, guarantees of medicine quality and safety and can be stored for two years at normal temperature.

The present invention relates to a gastrodine powder injection preparation. It is made up by using gastrodine as main component, its content is 30-99.9%, and water soluble medicinal auxiliary or water or their mixture. Said powder injection is good in stability, less in toxic side effect and extensive application range, and possesses the function of sedation, analgesia, hypnotic action, resisting vertigo, resisting convulsion, resisting epilepsy, improving cardio-carebral vascular function and resisting senility, etc.

The invention discloses a stable gastrodin crystal with high bioavailability for oral administration as well as a preparation method, a preparation and an application thereof. X-ray powder diffraction analysis, DSC and TG-DTA analysis and HPLC analysis show that the gastrodin crystal is a new crystal form. The preparation method comprises the steps that gastrodin crude products are dissolved with solvents for performing filtering separation, and ethyl acetate is added into the filtering liquid and is crystallized and separated to obtain a target. The preparation is a tablet or a capsule prepared by adding pharmaceutically acceptable carriers and auxiliaries into the gastrodin crystal. The application of the gastrodin crystal is an application of the stable gastrodin crystal with high bioavailability for oral administration in the preparation of medicines for preventing and / or treating cardiovascular and cerebrovascular diseases. The gastrodin crystal is high in purity, stable against light, moisture, heat and the like, high in stability in alkali solutions and high in in-vivo bioavailability; the preparation method is easy to carry out; compared with the existing crystallizing method, the used crystallized solvent has the advantages of less using amount of crystallized solvents, low production cost and easiness for industrial production; moreover, the content is more than 99 percent.

The invention discloses an enzyme-fermented rhizoma gastrodiae healthcare drink capable of improving sleep. The healthcare drink is characterized in that the healthcare drink is prepared through fermentation with an orientated enzyme fermentation process with fresh rhizoma gastrodiae serving as the main raw materials in cooperation with the auxiliary materials of fresh roses, coix seed, wheat, spina date seeds, mulberries and Chinese wolfberries, a traditional brewing process and the modern enzyme fermentation process are combined, the types of distiller's yeast and enzymes are screened in an orientated mode with the content of the functional component gastrodin as the index, functional components in the raw materials are extracted under the effect of microorganisms and enzymes, filtration is carried out with an aseptic filtration membrane, and then the product is obtained; in the formula, the raw materials include 30-50 parts of fresh rhizoma gastrodiae, 10-15 parts of spina date seeds, 10-20 parts of coix seed, 10-20 parts of wheat, 5-10 parts of fresh roses, 5-10 parts of mulberries and 5-10 parts of Chinese wolfberries. Functional verification experiments show that the healthcare drink has a significant sleep improving effect; meanwhile, the healthcare drink is unique in flavor, has the effects of improving sleep, tranquilizing the mind, strengthening the brain, maintaining beauty and prolonging the life, and is a good healthcare product for relieving the sub-health status of the human body and regulating the health balance of the body. The production process of the product is mature, large-scale production is convenient, and thus the product will have broad market application prospects.