The invention discloses a method for effectively preventing California sea cucumbers from disgorging and damaging intestines during long-distance transportation. The method comprises the following steps of 1, adjusting the
water temperature before transportation, namely, decreasing the
seawater temperature within a week step by step, decreasing the
seawater temperature by 1-2 DEG C every day and from 18 DEG C to 10 DEG C, temporarily culturing sea cucumbers, and preparing for transportation 3 days after stabilization; 2, performing controlling during the long-distance transportation; 3, putting the transported sea cucumbers in a
recirculating aquaculture system, and adjusting various parameters of
seawater; 4, adding
penicillin with a concentration of 5 ppm into the seawater after transportation, performing sufficient stirring, and immersing the sea cucumbers for 3-5 days; and 5, feeding the sea cucumbers by using compound probiotics and a composite
nutrient diet. The method is capable of effectively preventing the California sea cucumbers from disgorging and damaging intestines, is capable of optimizing intestines of the sea cucumbers and promoting digestive absorption of the sea cucumbers by utilizing an EM
bacterial agent and a bacillus probiotics
fermentation liquid, and is capable of effectively improving the
immunity and facilitating restoration of
vitality of the sea cucumbers.