155results about How to "Promote cloning" patented technology

A method and system of authenticating a computer resource such as an application or data on a mobile device uses a contactless token to provide multi-factor user authentication. User credentials are stored on the token in the form of private keys, and encrypted data and passwords are stored on the device. When an application user requires access to the resource an encrypted password is transmitted to and decrypted on the token using a stored key. An unencrypted data encryption key or password is then transmitted back to the device under the protection of a cryptographic session key which is generated as a result of strong mutual authentication between the device and the token.

Methods and compositions for introducing a nucleic acid into the genome of at least one cell of a multicellular organism are provided. In the subject methods, a Sleeping Beauty transposon that includes the nucleic acid is administered to the multicellular organism along with a source of a Sleeping Beauty transposase activity. Administration of the transposon and transposase results in integration of the transposon, as well as the nucleic acid present therein, into the genome of at least one cell of the multicellular organism The subject methods find use in a variety of different applications, including the in vivo transfer of genes for use in, among other applications, gene therapy applications.

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

A method and system of authenticating a computer resource such as an application or data on a mobile device uses a contactless token to provide multi-factor user authentication. User credentials are stored on the token in the form of private keys, and encrypted data and passwords are stored on the device. When application user requires access to the resource an encrypted password is transmitted to and decrypted on the token using a stored private key. An unencrypted data encryption key or password is then transmitted back to the device under the protection of a cryptographic session key which is generated as a result of strong mutual authentication between the device and the token.

The present invention provides modified primers for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified primers result in less template-independent non-specific product (primer dimer) compared to amplifications carried out using unmodified primers.

A method and system of conducting a cryptocurrency payment via a mobile device, using a contactless token to store and protect a user's secret key. A cryptocurrency wallet encrypted with the secret key is received by the mobile device from the token. A cryptocurrency payment instruction is received by the mobile device, prompting for a user credential to approve the instruction. In response the mobile device sends to the token a message comprising the encrypted wallet together with the payment instruction and the user credential. Using the secret key, the token then decrypts the cryptocurrency wallet from the encrypted wallet and creates a payment transaction by digitally signing the payment instruction, and transmitting the payment transaction to a cryptocurrency network or exchange. Confirmation of the transaction requires either a PIN, biometric or fingerprint on the mobile device, or authentication via button press, PIN or fingerprint on the token.

For the effective treatment of diseases such as cancer in which hIGF participates, there have been desired to be developed antibodies which strongly bind to both factors hIGF-I and hIGF-II and inhibit their functions and fragments of these antibodies. The present invention provides antibodies which have the ability to specifically bind to human IGF-I and IGF-II to thereby inhibit the functions of human IGF-I and IGF-II and have binding activity with a binding constant of 5×109 M−1 or more measured with a biosensor BIACORE. In addition, the present invention provides diagnostics, preventives and remedies for an hIGF-mediated disease and a disease showing pathological progressing due to abnormally promoted hIGF production, which use said antibodies.

The inventions is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the ‘hyper-translatable’ Cowpea Mosaic Virus (‘CPMV-HT’) based protein expression system.

For the effective treatment of diseases such as cancer in which hIGF participates, there have been desired to be developed antibodies which strongly bind to both factors hIGF-I and hIGF-II and inhibit their functions and fragments of these antibodies. The present invention provides antibodies which have the ability to specifically bind to human IGF-I and IGF-II to thereby inhibit the functions of human IGF-I and IGF-II and have binding activity with a binding constant of 5×109 M−1 or more measured with a biosensor BIACORE. In addition, the present invention provides diagnostics, preventives and remedies for an hIGF-mediated disease and a disease showing pathological progressing due to abnormally promoted hIGF production, which use said antibodies.

The present invention relates to novel muteins derived from tear lipocalin or a homologue thereof. In particular, the invention relates to a mutein of human tear lipocalin. The invention also refers to a corresponding nucleic acid molecule encoding such a mutein and to a method for its generation. The invention further refers to a method for producing such a mutein. Finally, the invention is directed to a pharmaceutical composition comprising such a lipocalin mutein as well as to various use of the mutein.

Methods and compositions for introducing a nucleic acid into the genome of at least one cell of a multicellular organism are provided. In the subject methods, a Sleeping Beauty transposon that includes the nucleic acid is administered to the multicellular organism along with a source of a Sleeping Beauty transposase activity. Administration of the transposon and transposase results in integration of the transposon, as well as the nucleic acid present therein, into the genome of at least one cell of the multicellular organism The subject methods find use in a variety of different applications, including the in vivo transfer of genes for use in, among other applications, gene therapy applications.

The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting re-combinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions and pharmaceutical formulations are provided that comprise replicon particles of the invention. Methods for generating an immune response or producing a pharmaceutical effect are also provided.

An embodiment of an apparatus for downloading and / or executing programs from a tool resident on a computer host is disclosed. The apparatus comprises an external flash memory storing a program, and a processor for validating the tool when detecting that the computer host connects to the apparatus. The processor permits the computer host to update the program of the external flash memory after determining that the tool has been successfully verified.

The invention provides a hair follicle stem cell separation culture method. The method utilizes an organ culture method, adopts the William's E complete medium to culture the hair follicle, separate and purify to get hair follicle stem cells. The method only uses very small skin materials, the rat whisker follicle is separated with a stereoscope through a micro separation method, then the whole whisker follicle is cultured, the hair follicle stem cells grow out from the projection part and form clone, so the cell clone forming efficiency is high, the purification is easy, long-term passage can be realized, the characteristics of the hair follicle stem cells are kept unchanged, and only one whisker follicle can obtain enough hair follicle stem cells for long-term use.

Temporary, user-authorized cloning of physical mobile phone functionality via a secure server can enable physical mobile phone features to be accessed and controlled by a user from a remote client. A secure server can include mobile phone registration information, enable secure access by users via a remote client, maintain communication and synchronization with the mobile phone, receive data associated with the physical mobile phone when is not in communication with at least one of a supporting telecommunication network and the secure server, and enable the physical mobile phone user to obtain secure communication with the secure server via a remote client, access and manage cloned mobile phone data and communicate with third parties. Physical mobile phone user access to the secure server and cloned mobile phone functionality with the remote client can be terminated once the physical mobile phone user logs off of the secure server from the remote client.

The invention provides a lymphocyte serum-free medium which comprises the following materials per liter: a basic culture medium, 10 mg of transferrin, 0.01 mg of sodium selenite, 110 mg of pyroracemic acid, 1.5 mg of ethanolamine, 10 mg of insulin, 10 to 30 mg of glutamine substitute, 300 mg of lipid-type bovine serum albumin, 0.001 mg of cupper sulfate, 0.0005 mg of ammonium meta-vanadate, 0.00005 mg of manganese chloride, 0.8 mg of zinc sulfate, 2 mg of Vitamin E, 100 mg of PHA, and 10 to 25 mg of an MHEPES / NaHCO3 buffering system. The invention further provides a preparation method and application to lymphocyte culture of the lymphocyte serum-free medium. The lymphocyte serum-free medium has the advantages that the repeatability of lymphocyte culture is high; poisoning and polluting of blood serum to lymphocyte are avoided; convenience is brought for differentiation of the lymphocyte, so as to obtain various mitosis phases.

The embodiment of the invention relates to a method for processing cloud resources and a physical node. The method comprises steps of determining to-be-processed cloud resources; acquiring depending cloud resources of the to-be-processed cloud resources and resource information groups of the to-be-processed cloud resources, wherein the resource information groups comprise resource information of the depending cloud resources; generating a resource topology template, wherein the resource topology template includes the resource information groups of the to-be-processed cloud resources, and depending relations of the to-be-processed cloud resources and the depending cloud resources; and according to the resource topology template, carrying out cloning or migrating processing on the to-be-processed cloud resources. According to the invention, cloning or migrating processing of multiple cloud resources can be finished automatically; it is ensured that attributes of the cloud resources willnot be changed; manual operation during the cloning or migrating processing of the single resource in the prior art is not required; the depending relations among the cloud resources can be copied; and copying efficiency is improved.

The present invention relates to recombinant fragments of C. difficile TcdA and TcdB that may be used in the development of vaccines against C. difficile associated disease. More particularly it relates to combinations comprising a ToxB-GT antigen and a TcdA antigen or a ToxA-GT antigen and a TcdB antigen.

The inventions is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the ‘hyper-translatable’ Cowpea Mosaic Virus (‘CPMV-HT’) based protein expression system.

The invention relates to application of a micromolecular heat shock protein gene improving stress resistance of oryza sativa. The gene improving various stress resistances of oryza sativa is Oryza sativa Heat Shock Protein18 (OsHSP18), with the nucleotide sequence being shown in SEQ ID NO:1 in a sequence table. The full-length coding sequence of the gene in SEQ ID NO:1 is inserted into a eukaryotic vector to obtain a eukaryotic recombinant plasmid over-expressed by the gene OsHSP18, and the eukaryotic recombinant plasmid is transformed into oryza sativa to obtain a transgenic plant over-expressed by the OsHSP18. Experiments show that the expression level of the gene OsHSP18 is obviously raised when oryza sativa is subjected to heat, drought, salt and low temperature stress, and the transgenic oryza sativa over-expressed by the OsHSP18 can enhance the resistance of the plant to various adversities (high temperature, drought, salt and cold damages) and does not have obvious impacts on agronomic traits such as plant height, tillering and the like. Thus, the gene can be applied to improvement of stress resistance of crops.

The invention discloses three SSR molecular markers cosegregated from a cucumber powdery mildew-resistant major QTL. The three SSR molecular markers are respectively named as SSR-N1, SSR-N2 and SSR-N3, wherein the nucleotide sequence of the SSR-N1 is shown in the SEQ ID No.1; the nucleotide sequence of the SSR-N2 is shown in the SEQ ID No.2; the nucleotide sequence of the SSR-N3 is shown in the SEQ ID No.3. The three SSR molecular markers disclosed by the invention all have high stability, can be simply and rapidly applied to auxiliary screening of powdery mildew-resistant individual plants at the cucumis sativus seedling stage, and lay a foundation for assistant breeding of powdery mildew-resistant molecular markers, so that the process of breeding cucumber powdery mildew-resistant molecules is accelerated greatly. Moreover, the cosegregated molecular markers lay a foundation for cloning of the cucumber powdery mildew-resistant major QTL.

A method for cloning and expressing a target gene by homologous recombination, and more particularly a method for cloning and expressing a target gene by homologous recombination, wherein a host cell transformed with a recombinant vector and a plasmid containing a recombinase system is introduced with a linear DNA fragment comprising a target gene and a sequence having homology to the recombinant vector. Because complicated genetic engineering steps, such as the restriction enzyme treatment and ligation of a vector and a target gene, are not required, the cloning of a gene can be performed without needing a high degree of skill, and enzyme cost can be reduced. The inventive method can be effectively used for the massive, high-speed cloning and protein expression of genes, and the disclosed pRMT-iTGR system can be used as an analytical means for improving high-efficiency recombinase.

The present invention relates to a therapeutic preventive agent that includes an angiogenic factor gene (such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and hypoxia inducible factor (HIF)) as its active ingredient, and the administration of such an agent into the targeted skin diseases-affected area.

The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting recombinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions and pharmaceutical formulations are provided that comprise replicon particles of the invention. Methods for generating an immune response or producing a pharmaceutical effect are also provided.

The invention belongs to the field of gene engineering, and relates to an improved promoter, a vector formed by the improved promoter and application of the improved promoter. The improved promoter isused for mutating nucleotide sequences from a -35 area to a -10 area in a promoter region into recognition sites of endonuclease. The improved promoter can be used for solving the problem that the cloning cannot be achieved, which is caused as a transcription or a translation product of an exogenous gene, which is promoted by a strong promoter existing in a vector adopting blue and white screening, possibly has toxicity to a host, can be used for avoiding the defect that the vector loses 1bp to 2bp at an enzyme digestion site to cause the frame shift mutation of a gene to generate false positive cloning, and can be used for eliminating the false negative phenomenon that a plate all has locus ceruleus, which is caused as an extraneous DNA (Deoxyribonucleic Acid) fragment is smaller and moreover a reading frame of the gene is not changed by the insertion of extraneous DNA.

The invention provides an application of 3'UTR of EBF1 (Early B-cell Factor 1) gene m RNA (Ribonucleic Acid) in the inhibition of gene expression, which comprises the following the steps that: after the nucleotide sequence with the number of EBF1 3'UTR and the reporter gene GFP (Green Fluorescent Protein) are recombined, are then integrated into a host genome through transgenosis, and are expressed along with the host genome; and compared with independent GFP, GFP with 3'UTR has remarkably-reduced protein products. The invention also provides the application of the 3'UTR of the EBF1 gene mRNA in the development adjustment of plants. The invention firstly finds that the 3'UTR of the EBFI gene has the function of negatively adjusting the gene expression of the plants.

The invention discloses a reference internal type dual-luciferase reporter vector and an application thereof. Firefly luciferase gene is fused between the renilla luciferase gene of the pRL-TK vector and the ampicillin resistance gene, the two luciferases are on the same vector, and the firefly luciferase gene is used as reference. The one-step binary bridging coupling long-distance polymerase chain reaction (PCR) and the Escherichia coli vivo homologous recombination method are utilized to place the firefly luciferase gene and the renilla luciferase gene on the same vector; and monoclonal sites are introduced in the 3' untranslated region of the renilla luciferase gene for conveniently cloning the target segment, thus the reference internal type dual-luciferase reporter vector can be constructed. The vector of the invention has the advantages of high repeatability, little multiple-pore variation, convenient operation and precise quantification. The vector of the invention is suitablefor the screening, identification and confirmation of the miRNA target molecule and also suitable for the quantitative analysis of the activity change of miRNA in cellular level.

The invention discloses two single nucleotide polymorphism molecular markers which can be used for high-throughput marker-assisted breeding of a powdery mildew resistance gene Pm5e and an applicationmethod of the markers in breeding. Pm5e is highly resistant or immune to current prevailing powdery mildew microspecies in China and has an important utilization value in breeding. These two detectionmarkers are KASP markers, can quickly and efficiently achieve high-throughput detection of the distribution of the wheat powdery mildew resistance gene Pm5e in wheat varieties and the distribution ofthe wheat powdery mildew resistance gene Pm5e in isolated populations, facilitate fine locating and cloning of Pm5e and can achieve marker-assisted selection of Pm5e in breeding populations. By meansof Pm5e, the powdery mildew resistance of wheat is improved, the impact on wheat yield is reduced, and the breeding efficiency of the wheat with powdery mildew resistance is improved.

PCT No. PCT / KR97 / 00057 Sec. 371 Date Dec. 2, 1997 Sec. 102(e) Date Dec. 2, 1997 PCT Filed Apr. 2, 1997 PCT Pub. No. WO97 / 37025 PCT Pub. Date Oct. 9, 1997The present invention relates to surface anchoring vectors, a method for preparation of foreign proteins onto a cell surface and use thereof, which uses outer cell membrane protein, ice nucleation protein (NIP) derived from Pseudomonas syringae, a gram-negative bacterium.

A method and system of authenticating a computer resource such as an application or data on a mobile device uses a contactless token to provide multi-factor user authentication. User credentials are stored on the token in the form of private keys, and encrypted data and passwords are stored on the device. When application user requires access to the resource an encrypted password is transmitted to and decrypted on the token using a stored private key. An unencrypted data encryption key or password is then transmitted back to the device under the protection of a cryptographic session key which is generated as a result of strong mutual authentication between the device and the token.