290 results about "Reading frame" patented technology

A multigene expression vehicle (MGEV) consisting essentially of a polynucleotide comprising 2 to 8 domain segments, D, each domain encoding a functional protein, each domain being joined to the next in a linear sequence by a Linker (L) segment encoding a Linker peptide, the D and L segments all being in the same reading frame, and at least one of the domains is not a type two protease inhibitor.

A fully automated, user-independent method is described for computer-mediated interpretation of data derived by mass spectrometry of an experimental peptide to identify and characterize a corresponding peptide sequence in a peptide database. The method identifies the corresponding sequence if it is present in the database, without the need for a skilled observer to choose from amongst a list of possible matches. By using an automated back-read process, the present method can uniquely identify a corresponding peptide sequence in a database based on a single matching peptide sequence. The method also permits mapping of mass spectral data to sequences in peptide or nucleotide databases for unambiguous identification of exons; determining a correct reading frame; identifying artefacts and errors in sequences; identifying mutations and polymorphisms; identifying post-translational modifications; and identifying exon-intron boundaries.

The invention discloses a multi-functional automotive central information integration system for realizing filtration and forwarding between two CANs. Content displayed by an LCD comprises vehicle running state, vehicle body state display, navigation and position display and reversing backsight display. The method for realizing data filtration and forwarding between the two CANs comprises the following steps of: a, judging whether CAN data forwarding is idle and whether data exist in an FIFO buffer of CAN information, executing step e if .f., otherwise, sequentially executing the next step; b, reading frame data from the buffer, judging whether a data frame needs to be forwarded, executing step d if .f., otherwise, sequentially executing the next process; c, setting a forwarding busy flag bit, transferring the data frame to another CAN interface, clearing the forwarding busy flag bit after the data frame is sent, and returning to the step a; d, adjusting a data sending pointer, and processing the data frame; and e, executing the corresponding control display in a central information integration system according to frame definition (comprising parameter groups during data filtration and forwarding and corresponding parameter definition).

Methods for determining whether a molecule affects a disorder are provided. A cell from an organism is contacted with the molecule, or the molecule is expressed within the cell. A determination is made as to whether the RNA or protein expression in the cell of at least one open reading frame is changed relative to the expression of the reading frame in the absence of the molecule. Each such open reading frame is regulated by a promoter native to SEQ ID NOS: 5-9, 11-12, 14, 16, 18, 20-21, 23, 25, 27, 29, 31, 33 or homologs of the foregoing. A determination is made as to whether the molecule affects the disorder when the RNA or protein expression of the at least one reading frame is changed. Alternatively, a determination is made that the molecule does not affect the disorder when the RNA or protein expression of the at least one reading frame is unchanged.

The invention provides a production method for Fbxo40 gene knockout pigs. The production method comprises the steps that a CRISPR-Cas9 targeting vector and a PGK-Neo resistant gene are jointly transfected into a porcine embryonic fibroblast to obtain a G418-resistant positive monoclonal cell, an Fbxo40 gene in the positive monoclonal cell generates insertion or deletion mutation, a reading frame generates frame shift and stops in advance, then the obtained cell is cloned to serve as a donor cell for nuclear transfer, and an oocyte serves as a receptor oocyte for nuclear transfer; cloned embryos are obtained through a somatic cell nuclear transfer technique, the high-quality cloned embryos are transferred into the fallopian tube of an oestrous sow, and the Fbxo40 gene knockout pigs are obtained through whole-period development. According to the production method, the Fbxo40 knockout pigs are efficiently obtained through a CRISPR-Cas9 gene editing technique at the low cost, and animal models are supplied to research of muscle development and diseases related to the muscles.

Diagnostically relevant polypeptides and fusion proteins comprising an amino acid sequence which originates from cytomegalovirus and corresponds to a region of the major DNA-binding protein or of the C-terminal region of the tegument protein pp150 fused with at least one further fragment from another antigenic protein of cytomegalovirus are disclosed. The major DNA-binding protein is encoded by the reading frame UL57. The poly-peptides and fusion proteins according to the invention can be used in an advantageous manner in diagnostic tests and methods for the detection of IgM antibodies against cytomegalovirus.

The present invention relates to an artificially synthesized gene optiHA containing codons for chicken partial tropism. Its reading frame contains 1707 bp nucleotides and encodes a total of 568 amino acids. The gene is compatible with H5 subtype highly pathogenic avian influenza virus A / Goose / GuangDong / 1 / 96(H5N1)[GD / 1 / 96(H5N1)]hemagglutinin (HA) gene has a nucleotide homology rate of 70%, an amino acid homology rate of 100%, and encodes the H5 subtype Hemagglutinin (HA) protein of avian influenza virus GD / 1 / 96 (H5N1). The invention also relates to the application of the gene as an immunogenic gene of H5 subtype influenza DNA vaccine and other genetic engineering vaccines.

A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the reading frame to a sequence (2) located upstream from the first sequence. Sequence (2) codes for at least part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein, which contains the interaction site of Fha and heparin and the secretion domain. This Fha protein is under the control of a promoter recognized by the cell polymerases of B. pertussis and is inserted into a B. pertussis cell culture, is expressed in the culture and excreted into the cell culture medium. The invention uses both the abilities of Bordetella and particularly B. pertussis to secrete or surface expose the heterologous polypeptide fused to the Fha portion corresponding to sequence (2), which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from other Bordetella proteins.

The invention discloses three novel cotton ABF / AREB / ABI5 / DPBF type transcription factors GhABF2, GhABF3 and GhABI5, and coding genes and application thereof. Amino acid sequences of the GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 2, 5 and 8 respectively. Nucleotide sequences of the coding genes GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 1, 4 and 7 respectively. The invention also discloses the structural characteristics on genomic deoxyribonucleic acid (DNA) and a ribonucleic acid (RNA) editing law of reading frame regions of the coding genes GhABF2, GhABF3 and GhABI5. The invention also discloses the cotton expression characteristics and yeast expression characteristics of the three genes and the characteristic that the three genes can improve the drought resistance of arabidopsis thaliana. The genes provide gene resources for culturing a plant variety resistant to abiotic stress, and have great significance for improving the abiotic stress resistance of plants.

The present invention relates to a versatile method of identifying protein coding DNA sequences (genes) useful as drug targets in a genome using specially developed software GeneDecipher, said method comprising steps of generating peptide libraries from the known genomes with peptide of length ‘N’ computationally arranged in an alphabetical order, artificially translating the test genome to obtain a polypeptide corresponding to each reading frame, converting each polypeptide sequence into an alphanumeric sequence one corresponding to each reading frame on the basis of overlappings with the peptide libraries, training Artificial Neural Network (ANN) with sigmoidal learning function to the alphanumeric sequence, deciphering the protein coding regions in the test genome, thus, identifying longer streches of peptides mapping to large number of known genes and their corresponding proteins and lastly, a method of the management of the diseases caused by the pathogenic organisms comprising a step of evaluation of the proposed drug candidate by inhibiting the functioning of one or more proteins identified by the steps of the invention.

The invention relates to a computer vision detection technology-based method for detecting and identifying a QR (Quick Response) code. According to the method, a computer, a digital camera, a light supplementing device and an acousto-optical prompt device are mainly comprised, wherein a video signal of the digital camera is output to the computer; after receiving input of a video sensor, the computer controls the light supplementing device and the acousto-optical prompt device; a QR code intelligent detection module is installed in the computer and used for detecting, extracting and decoding a QR code graph through circularly reading frame data in a scanning video; and if successful, a user is prompted through a buzzer. According to location characteristic detection of the QR code, the computer vision detection technology-based method for detecting and identifying the QR code solves the problem of extracting and decoding of the QR code which is defected and blocked by a locator in a complex illumination background, has the characteristics of low cost, no complex mechanical device resulting in faults easily and capability of utilizing the traditional intelligent equipment effectively and expansion of the traditional functions with an algorithm module.

A fully automated, user-independent method is described for computer-mediated interpretation of data derived by mass spectrometry of an experimental peptide to identify and characterize a corresponding peptide sequence in a peptide database. The method identifies the corresponding sequence if it is present in the database, without the need for a skilled observer to choose from amongst a list of possible matches. By using an automated back-read process, the present method can uniquely identify a corresponding peptide sequence in a database based on a single matching peptide sequence. The method also permits mapping of mass spectral data to sequences in peptide or nucleotide databases for unambiguous identification of exons; determining a correct reading frame; identifying artefacts and errors in sequences; identifying mutations and polymorphisms; identifying post-translational modifications; and identifying exon-intron boundaries.

This invention relates to seeds of plant, plants themselves and cells of such plants which comprise a heterologous gene coding for a plant (such as nasturtium (Tropaeolum majus) or Crambe abyssinica) fatty acid elongase (FAE) gene or allelic variant thereof, or combinations of one or both of these FAE genes with an Arabidopsis fatty acid elongase 1 (FAE1) gene, in co-transformation, in reading frame alignment with a promoter capable of increasing expression of said gene(s), when said transformed plant cell is in a seed, said plant cell or seed being capable of producing an increase in proportion of a very long chain monounsaturated or saturated fatty acids when compared with the proportions of said fatty acids in a control plant cell or seed lacking said heterologous FAE gene or genes. The invention also relates to combinations of these fatty acid elongase genes by traditional crossing, sufficient to increase the proportion of very long chain monounsaturated or saturated fatty acids in the seed oil of the progeny compared to the proportion of said fatty acids in either of the parental lines.

An intronic, self-splicing riboswitch is configured for enzyme-product specificity by introducing an appropriate aptamer. This then provides a sensing-expression construct, whereby the presence of an enzyme product in the cell triggers self-splicing of the intron sequence to restore the reading frame of the reporter gene and as such to drive expression of the gene product. The sensing construct expresses a protein which marks the cell or permits its growth or survival in or on an otherwise selective media. In this way, introduction or the presence of such product sensing-reporter constructs in cells can be harnessed to provide a multi-parallel rapid screening of cells or libraries for desirable enzyme variants.

The invention relates to the biological technical field, and particularly discloses a replication-defective human adenovirus type 55 vector and a preparation method and an application thereof. The replication-defective human adenovirus type 55 vector is prepared by the following method: E1 and E3 genes of Ad55 are knocked out, an open reading frame 6 or open reading frames 2, 3, 4, 6 and 6 / 7 of an E4 gene in an Ad55 genome are changed into corresponding reading frames of an Ad5 genome, and in addition, an exogenous gene expression frame is integrated in an E1 gene region of the Ad55. The vector can be produced in large quantities in 293, PerC6 and other auxiliary cell lines, and can be concentrated and purified by density gradient centrifugation; normal human cells do not have the replication capacity, thereby having an attenuated phenotype; in addition, the vector can efficiently express an exogenous gene in target cells. The vector can be used as a vaccine or a gene therapy vector, and can also be applied in development of drugs and neutralizing antibodies and in a trace reporting system.

Duchenne muscular dystrophy (DMD) is an inherited X-linked disease caused by mutations in the gene encoding dystrophin, a protein required for muscle fiber integrity. The disclosure reports CRISPR / Cas9-mediated gene editing (Myo-editing) is effective at correcting the dystrophin gene mutation in the mdx mice, a model for DMD. Further, the disclosure reports optimization of germline editing of mdx mice by engineering the permanent skipping of mutant exon (exon 23) and extending exon skipping to also correct the disease by post-natal delivery of adeno-associate virus (AAV). AAV-mediated Myo-editing can efficiently rescue the reading frame of dystrophin in mdx mice in vivo. The disclosure reports means of Myo-editing-mediated exon skipping has been successfully advanced from somatic tissues in mice to human DMD patients-derived iPSCs (induced pluripotent stem cells). Custom Myo-editing was performed on iPSCs from patients with differing mutations and successfully restored dystrophin protein expression for all mutations in iPSCs-derived cardiomyocytes.

This invention open a cold tolerance of Serratia sand Habitat-chuen (Serratia fonticola) xjF1 CGMCC No.1971, through the strain characteristics, enzyme production conditions and the nature of enzyme research, proved a new phospholipase A1 gene consists of two open plA and plS reading frame, was certified plA gene coding phospholipase A1, plS gene coding phospholipase A1 auxiliary protein. This invention relates to the gene containing the recombinant plasmid and reorganization cell, the gene expression products phospholipase A1 in the show activity under low-temperature conditions, can be used in industries such as oil refining process can be simplified production process, lowering energy consumption, improve product quality , protecting the environment, reduce production costs, have important practical significance.

The present invention provides a cyclic RNA preferable for carrying out rotary protein translation in which translation domains other than that of the target protein are sufficiently short and translation efficiency is high, and a method for producing protein that uses this cyclic RNA as template. More specifically, the present invention provides a cyclic RNA that encodes a protein, has a full-length number of bases that is equal to or greater than 102 and is a multiple of 3, has at least one start codon, does not have a stop codon in the same reading frame as the start codon, and does not contain an internal ribosome entry site (IRES). In addition, the present invention provides a method for producing protein in a eukaryotic cell expression system that consists of using the aforementioned cyclic RNA as template and expressing a protein encoded by that cyclic RNA.

The invention relates to nucleic acid expression cassettes and vectors comprising these expression cassettes, where two or more transgenes can be expressed. In general, the expression cassettes orient at least two of the transgenes in opposite directions with respect to their reading frames. In one aspect, vectors comprising these expression cassettes advantageous can be used to provide relatively equal levels of expression of each of the two or more transgenes. In particular examples, the vectors can be used in methods to treat disease by allowing multiple therapeutic polypeptides to be expressed in the same cell.

The present invention is one kind of cryophilous protease gene mcp01 and its preparation process, and belongs to the field of biotechnology. The cryophilous proteinase gene mcp01 has one genome DNA segment of 2676 bp containing one opened reading frame with 2508 nucleotides. The opened reading frame is the gene encoding protease MCP-01, and the protease MCP-01 gene mcp01 expressed cryophilous protease has optimal enzyme activity temperature of about 35 deg.c and optimal enzyme pH of 9.0. The present invention may be applied in low temperature preservation of meat, dairy product processing, detergent production, etc.

A computer-based reading-fluency training tool for helping a reader increase her / his reading speed and comprehension. In some embodiments, the training tool includes a reading frame that displays multiple lines of multiline reading material. The displayed multiple lines are masked, and the training tool moves a guided window through the multiple lines in a controlled manner to temporarily unmask a portion of the multiple lines to guide the reader through the reading material. The multiline reading material can be text-based material for reading-comprehension sessions. The multiline material can be symbol-based material for visual-perception training

The invention provides a polygene coexpression system which comprises an expression vector of disulfide-bond target protein coding genes, and one or more than one expression vector of coding genes of an enzyme and / or chaperonin which has the effect of promoting the functionalization of disulfide-bond target protein; the coding genes of the enzyme and / or chaperonin are constructed in one reading frame of the expression vector in a form of fusion genes, or two or more than two of the coding genes of the enzyme and / or chaperonin are respectively constructed in different reading frames of the expression vector; the coding genes of the target protein are constructed in an expression vector which contains different resistant genes from the expression vector of the enzyme and / or chaperonin. The invention also provides a preparation method of the polygene coexpression system, and a method for preparing soluble functional target protein by using the preparation method. The polygene coexpression system of the invention enables the expressed disulfide bond-containing protein to form correct disulfide bonds, to fold into correct spatial conformation, and thus to dissolve in a cracking supernatant with maintained corresponding biological functions or activity.

The invention discloses a GII.P12 / GII.3 recombinant norovirus genome amplification primer and an amplification method. The amplification method comprises the following steps: by taking six pairs of amplification primers as forward and reverse primers of amplification primers respectively, and by taking RNA of GII.P12 / GII.3 recombinant norovirus as a template, performing RT-PCR amplification so as to obtain amplification products respectively, performing nucleotide sequencing on the amplification products by using the six amplification primers and two sequencing primers II.12-Seq1R and II.3-Seq6F, and then carrying out jointing and comparison, thereby obtaining full-length sequences of a GII.P12 / GII.3 recombinant norovirus genome. For the GII.P12 / GII.3 recombinant norovirus which occurs frequently in China, a sectional amplification strategy of '4+1+1'is designed according to three opening reading frames included in the genome, corresponding amplification primers are designed according to conserved regions, the amplification primers are applied to practical detection samples, and GII.P12 / GII.3 recombinant norovirus genome sequences can be acquired. The GII.P12 / GII.3 recombinant norovirus genome amplification primer and the amplification method can be widely applied to fields such as medical treatment and public health and inspection and quarantine with norovirus detection requirements, and related scientific fields.

Methods of modifying a dystrophin gene are disclosed, for restoring dystrophin expression within a cell having an endogenous frameshift mutation within the dystrophin gene. The methods comprising introducing a first cut within an exon of the dystrophin gene creating a first exon end, wherein said first cut is located upstream of the endogenous frameshift mutation; and introducing a second cut within an exon of the dystrophin gene creating a second exon end, wherein said second cut is located downstream of the frameshift mutation. Upon joining / ligation of said first and second exon ends dystrophin expression is restored, as the correct reading frame is restored. Reagents and uses of the method are also disclosed, for example to treat a subject suffering from muscular dystrophy.

The invention discloses an electric meter reading frame. The electric meter reading frame comprises a display screen, an operation frame, a lifting module and an instrument base; a clamping push plateand a spring piece are arranged in the instrument base; the operation frame comprises an operation plate and a hand holding handle; the lifting module comprises a guide rod, a guide sleeve, a rack, agear, an adjusting handle and a stop piece; the bottom of the guide rod is connected with the operation plate; the top of the guide rod is connected with the instrument base; the rack is connected with the guide rod through the guide sleeve, and can slide up and down in the length direction of the guide rod through the guide sleeve; the stop piece comprises an adjusting bar; and a locking bolt isinserted in a positioning hole, and can fixedly lock the adjusting handle and the adjusting bar. The electric meter reading frame is simple in structure and convenient to use, can effectively fix a meter reading instrument on the instrument base through the clamping push plate and a limiting pressure plate, can enable the meter reading instrument to reach a farther position through the adjustinghandle driving the rack to lift, has no need to enable workers to climb for meter reading, and improves the working efficiency and the use safety.

The invention discloses a replicative HBV (Hepatitis B Virus) vector carrying a foreign gene and a recombinant HBV generated after transfection and a corresponding preparation method and an application. The vector separates overlaying genes C and P on an HBV genome by a molecular cloning technique based on originally expressed HBV plasmid to respectively form an integral opened reading frame where a protein translation starting sequence or a protease enzyme cutting site is inserted to respectively guide expression of foreign gene and gene P. The replicative HBV vector carrying the foreign gene transiently transfecting hepatoma carcinoma cell secretes the recombinant HBV. The recombinant HBV prepared from the HBV vector transfection cells provided by the invention can express the foreign gene and maintain the replicative and infecting capacity. The invention is suitable for constructing an HBV chronic infection animal model, an HBV cell model with cccDNA stably and automatically replicated and a traceable HBV strain, researching a molecular mechanism of HBV infection, replication, packaging and the like, and screening anti-HBV novel medicines.

This invention provides nucleic acids containing sequences from a TCRγ transcript from prostate epithelial cells and many breast cancer cells and a T-cell receptor gamma Alternate Reading frame Protein (“TARP”) expressed from the translation of those sequences. Vaccines made from TARP are useful in raising immune responses to cells in which the protein is expressed, including prostate cancer cells and cells of many breast cancers. The invention also provides methods for diagnosing the presence of prostate cancer and TARP-expressing breast cancers, as well as methods of administering TARP and nucleic acids encoding TARP to subjects.

A novel vaccine that can induce sufficiently high cell-mediated immunity is disclosed. The vaccine of the present invention contains, as an effective component, a polypeptide comprising a tandem repeat structure in which an MHC class I epitope region derived from an antigen protein and a spacer sequence are linked to each other alternately and repeatedly at least three times, or a recombinant vector which comprises a polynucleotide encoding said polypeptide and is capable of expressing said polypeptide in vivo. The spacer sequence is, for example, a sequence generated as an amino acid sequence inevitably encoded by a single base sequence which is designed such that the MHC class I epitope region derived from the antigen protein, an MHC class II epitope region derived from the antigen protein, and at least one higher-order-structure-stabilizing region are encoded by different reading frames in said single base sequence.

The invention discloses a novel preparation technology of targeting antitumor fusion protein (lipid peroxidation). The novel preparation method comprises the steps of building a fusion expression protein which takes the glutathione S-transferase A (TrxA)-His-tag (His6-Tag)-SUMO protease recognition substrate as a protein soluble expression auxiliary fragment and takes LHRH (luteinizing hormone releasing hormone)-PEA (phenylethylamine) trans-cell penetrating peptides-ONC (LPO for short) as a target segment, connecting the two proteins with each other by a correct read frame and carrier positive sequence and converting to enter into expression bacteria, finally building fusion protein which is connected with six expression substances in series, crudely extracting, carrying out metal chelating medium purification in the presence of imidazole, and carrying out SUMO protease digestion. Compared with the LPO prepared by a conventional method, the LPO prepared by the novel preparation technology disclosed by the invention can obviously improve the inhibiting effect on the tumor cell lines such as colon cancer HT-29 cells, ovarian cancer OVCAR3 cells, cervical adenocarcinoma HeLa cells and liver cancer HepG-2 cells.