The invention discloses a
pseudorabies virus gene deleted strain, and a preparation method of an
inactivated vaccine for porcine
pseudorabies. The
gene deleted strain is a
pseudorabies virus gE
gene deleted strain, which is constructed through
reading frame frameshift mutation caused by deletion of a plurality of basic groups from a wild-type pseudorabies
virus (PRV) strain gE
gene sequence. The preparation method includes the following steps: constructing the pseudorabies virus gE gene deleted strain; domesticating a porcine testicular
cell and subjecting the same to
suspension culture; inoculating the porcine testicular
cell with the pseudorabies virus gE gene deleted strain, and harvesting a
cell culture when 80-90% of the cell has lesions to obtain cell
venom containing supernatant; taking the supernatant to measure the valence, and inactivating the qualified cell
venom in a
sterilization container; and mixing the inactivated cell
venom with an
adjuvant to obtain the
inactivated vaccine for the porcine pseudorabies. The prepared
inactivated vaccine for the porcine pseudorabies has high
immunogenicity, long immunization period, and no side effects after immunization, is safe andreliable, and can effectively prevent infections of pseudorabies virus epidemic strains.